This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.94 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 84,513 (3.3%) Reads that were too short: 21,267 (0.8%) Reads written (passing filters): 63,246 (2.5%) Total basepairs processed: 766,381,518 bp Total written (filtered): 17,270,185 bp (2.3%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 84513 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2821 621.6 0 2821 7 727 621.6 0 727 8 26078 621.6 0 26078 9 20601 621.6 0 20601 10 49 621.6 0 49 11 2 621.6 0 2 12 5 621.6 0 5 13 5 621.6 0 5 14 4 621.6 0 4 15 7 621.6 0 7 16 4 621.6 0 4 17 15 621.6 0 15 18 12 621.6 0 12 19 13 621.6 0 13 20 6 621.6 0 6 21 4 621.6 0 4 22 4 621.6 0 4 23 4 621.6 0 4 24 11 621.6 0 11 25 9 621.6 0 9 26 34 621.6 0 34 27 48 621.6 0 48 28 38 621.6 0 38 29 15 621.6 0 15 30 5 621.6 0 5 31 7 621.6 0 7 32 4 621.6 0 4 33 17 621.6 0 17 34 7 621.6 0 7 35 5 621.6 0 5 36 1 621.6 0 1 37 6 621.6 0 6 38 7 621.6 0 7 39 10 621.6 0 10 40 13 621.6 0 13 41 9 621.6 0 9 42 28 621.6 0 28 43 24 621.6 0 24 44 8 621.6 0 8 45 13 621.6 0 13 46 11 621.6 0 11 47 6 621.6 0 6 48 9 621.6 0 9 49 9 621.6 0 9 50 14 621.6 0 14 51 10 621.6 0 10 52 14 621.6 0 14 53 24 621.6 0 24 54 22 621.6 0 22 55 15 621.6 0 15 56 16 621.6 0 16 57 23 621.6 0 23 58 81 621.6 0 81 59 245 621.6 0 245 60 1600 621.6 0 1600 61 1081 621.6 0 1081 62 1222 621.6 0 1222 63 27 621.6 0 27 64 21 621.6 0 21 65 50 621.6 0 50 66 243 621.6 0 243 67 100 621.6 0 100 68 74 621.6 0 74 69 5 621.6 0 5 70 4 621.6 0 4 71 16 621.6 0 16 72 24 621.6 0 24 73 8 621.6 0 8 74 6 621.6 0 6 75 15 621.6 0 15 76 35 621.6 0 35 77 326 621.6 0 326 78 124 621.6 0 124 79 187 621.6 0 187 80 33 621.6 0 33 81 70 621.6 0 70 82 60 621.6 0 60 83 25 621.6 0 25 84 74 621.6 0 74 85 36 621.6 0 36 86 28 621.6 0 28 87 9 621.6 0 9 88 15 621.6 0 15 89 6 621.6 0 6 90 17 621.6 0 17 91 24 621.6 0 24 92 20 621.6 0 20 93 28 621.6 0 28 94 31 621.6 0 31 95 20 621.6 0 20 96 8 621.6 0 8 97 17 621.6 0 17 98 21 621.6 0 21 99 22 621.6 0 22 100 16 621.6 0 16 101 19 621.6 0 19 102 9 621.6 0 9 103 20 621.6 0 20 104 24 621.6 0 24 105 10 621.6 0 10 106 10 621.6 0 10 107 17 621.6 0 17 108 22 621.6 0 22 109 32 621.6 0 32 110 37 621.6 0 37 111 159 621.6 0 159 112 371 621.6 0 371 113 141 621.6 0 141 114 288 621.6 0 288 115 325 621.6 0 325 116 825 621.6 0 825 117 42 621.6 0 42 118 17 621.6 0 17 119 21 621.6 0 21 120 13 621.6 0 13 121 19 621.6 0 19 122 20 621.6 0 20 123 53 621.6 0 53 124 24 621.6 0 24 125 37 621.6 0 37 126 23 621.6 0 23 127 20 621.6 0 20 128 25 621.6 0 25 129 32 621.6 0 32 130 32 621.6 0 32 131 27 621.6 0 27 132 26 621.6 0 26 133 39 621.6 0 39 134 22 621.6 0 22 135 47 621.6 0 47 136 28 621.6 0 28 137 51 621.6 0 51 138 79 621.6 0 79 139 88 621.6 0 88 140 34 621.6 0 34 141 28 621.6 0 28 142 17 621.6 0 17 143 53 621.6 0 53 144 38 621.6 0 38 145 79 621.6 0 79 146 37 621.6 0 37 147 46 621.6 0 46 148 23 621.6 0 23 149 14 621.6 0 14 150 20 621.6 0 20 151 26 621.6 0 26 152 19 621.6 0 19 153 27 621.6 0 27 154 25 621.6 0 25 155 13 621.6 0 13 156 23 621.6 0 23 157 17 621.6 0 17 158 29 621.6 0 29 159 28 621.6 0 28 160 93 621.6 0 93 161 29 621.6 0 29 162 37 621.6 0 37 163 53 621.6 0 53 164 75 621.6 0 75 165 117 621.6 0 117 166 93 621.6 0 93 167 91 621.6 0 91 168 37 621.6 0 37 169 25 621.6 0 25 170 29 621.6 0 29 171 32 621.6 0 32 172 25 621.6 0 25 173 21 621.6 0 21 174 25 621.6 0 25 175 26 621.6 0 26 176 24 621.6 0 24 177 27 621.6 0 27 178 41 621.6 0 41 179 74 621.6 0 74 180 57 621.6 0 57 181 141 621.6 0 141 182 47 621.6 0 47 183 33 621.6 0 33 184 114 621.6 0 114 185 136 621.6 0 136 186 83 621.6 0 83 187 33 621.6 0 33 188 24 621.6 0 24 189 57 621.6 0 57 190 185 621.6 0 185 191 221 621.6 0 221 192 144 621.6 0 144 193 85 621.6 0 85 194 21 621.6 0 21 195 41 621.6 0 41 196 37 621.6 0 37 197 49 621.6 0 49 198 53 621.6 0 53 199 32 621.6 0 32 200 33 621.6 0 33 201 29 621.6 0 29 202 34 621.6 0 34 203 201 621.6 0 201 204 61 621.6 0 61 205 93 621.6 0 93 206 57 621.6 0 57 207 241 621.6 0 241 208 201 621.6 0 201 209 164 621.6 0 164 210 130 621.6 0 130 211 48 621.6 0 48 212 91 621.6 0 91 213 42 621.6 0 42 214 55 621.6 0 55 215 88 621.6 0 88 216 89 621.6 0 89 217 185 621.6 0 185 218 78 621.6 0 78 219 51 621.6 0 51 220 28 621.6 0 28 221 36 621.6 0 36 222 39 621.6 0 39 223 53 621.6 0 53 224 173 621.6 0 173 225 150 621.6 0 150 226 119 621.6 0 119 227 83 621.6 0 83 228 71 621.6 0 71 229 63 621.6 0 63 230 51 621.6 0 51 231 94 621.6 0 94 232 123 621.6 0 123 233 84 621.6 0 84 234 100 621.6 0 100 235 183 621.6 0 183 236 433 621.6 0 433 237 416 621.6 0 416 238 380 621.6 0 380 239 149 621.6 0 149 240 76 621.6 0 76 241 66 621.6 0 66 242 79 621.6 0 79 243 139 621.6 0 139 244 265 621.6 0 265 245 3736 621.6 0 3736 246 1655 621.6 0 1655 247 3217 621.6 0 3217 248 53 621.6 0 53 249 44 621.6 0 44 250 38 621.6 0 38 251 47 621.6 0 47 252 54 621.6 0 54 253 44 621.6 0 44 254 93 621.6 0 93 255 88 621.6 0 88 256 67 621.6 0 67 257 43 621.6 0 43 258 49 621.6 0 49 259 92 621.6 0 92 260 219 621.6 0 219 261 207 621.6 0 207 262 279 621.6 0 279 263 181 621.6 0 181 264 186 621.6 0 186 265 1879 621.6 0 1879 266 262 621.6 0 262 267 219 621.6 0 219 268 70 621.6 0 70 269 36 621.6 0 36 270 196 621.6 0 196 271 219 621.6 0 219 272 290 621.6 0 290 273 78 621.6 0 78 274 43 621.6 0 43 275 35 621.6 0 35 276 86 621.6 0 86 277 85 621.6 0 85 278 163 621.6 0 163 279 77 621.6 0 77 280 86 621.6 0 86 281 77 621.6 0 77 282 82 621.6 0 82 283 80 621.6 0 80 284 144 621.6 0 144 285 112 621.6 0 112 286 94 621.6 0 94 287 90 621.6 0 90 288 51 621.6 0 51 289 47 621.6 0 47 290 46 621.6 0 46 291 67 621.6 0 67 292 76 621.6 0 76 293 83 621.6 0 83 294 61 621.6 0 61 295 70 621.6 0 70 296 73 621.6 0 73 297 69 621.6 0 69 298 115 621.6 0 115 299 90 621.6 0 90 300 81 621.6 0 81 301 81 621.6 0 81 Finished in 3.41 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 63,246 Reads with adapters: 24,099 (38.1%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 24,082 (38.1%) Total basepairs processed: 17,270,185 bp Total written (filtered): 6,550,619 bp (37.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 24099 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 18 0.0 3 0 0 12 3 3 19 330 0.0 3 0 291 19 8 12 20 21148 0.0 4 19654 1177 230 64 23 21 76 0.0 4 8 60 4 1 3 22 2395 0.0 4 2217 138 22 15 3 23 70 0.0 4 4 3 0 35 28 24 1 0.0 4 0 0 0 0 1 25 13 0.0 4 0 0 0 8 5 56 1 0.0 4 0 0 0 0 1 57 3 0.0 4 0 0 0 0 3 65 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 1 92 2 0.0 4 0 0 0 0 2 99 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 103 2 0.0 4 0 0 0 0 2 113 1 0.0 4 0 0 0 0 1 119 2 0.0 4 0 0 0 0 2 120 1 0.0 4 0 0 0 0 1 127 15 0.0 4 0 0 0 0 15 128 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 176 1 0.0 4 1 189 1 0.0 4 0 1 216 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 266 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 269 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.89 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 24,082 Reads with adapters: 6,564 (27.3%) Reads written (passing filters): 24,082 (100.0%) Total basepairs processed: 6,550,619 bp Total written (filtered): 6,460,755 bp (98.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6564 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.4% C: 8.9% G: 85.5% T: 3.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 12 376.3 0 12 4 8 94.1 0 8 7 2 1.5 0 2 8 1 0.4 0 1 9 2492 0.1 0 265 2227 10 2986 0.0 1 207 2779 11 904 0.0 1 30 874 12 24 0.0 1 0 24 13 7 0.0 1 0 7 14 1 0.0 1 0 1 150 8 0.0 1 5 3 153 2 0.0 1 1 1 169 1 0.0 1 1 208 24 0.0 1 19 5 209 2 0.0 1 2 210 1 0.0 1 1 223 85 0.0 1 80 5 224 3 0.0 1 2 1 227 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.