This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.24 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 76,937 (3.0%) Reads that were too short: 17,816 (0.7%) Reads written (passing filters): 59,121 (2.3%) Total basepairs processed: 766,381,518 bp Total written (filtered): 16,722,105 bp (2.2%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 76937 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3433 621.6 0 3433 7 558 621.6 0 558 8 23482 621.6 0 23482 9 25937 621.6 0 25937 10 22 621.6 0 22 11 8 621.6 0 8 12 6 621.6 0 6 13 1 621.6 0 1 14 4 621.6 0 4 15 3 621.6 0 3 17 1 621.6 0 1 18 3 621.6 0 3 19 2 621.6 0 2 20 2 621.6 0 2 22 1 621.6 0 1 23 6 621.6 0 6 24 3 621.6 0 3 25 1 621.6 0 1 26 3 621.6 0 3 27 6 621.6 0 6 28 4 621.6 0 4 29 4 621.6 0 4 30 3 621.6 0 3 31 1 621.6 0 1 32 9 621.6 0 9 33 8 621.6 0 8 34 3 621.6 0 3 35 2 621.6 0 2 36 2 621.6 0 2 37 6 621.6 0 6 38 3 621.6 0 3 39 6 621.6 0 6 40 5 621.6 0 5 41 6 621.6 0 6 42 4 621.6 0 4 43 12 621.6 0 12 44 4 621.6 0 4 45 8 621.6 0 8 46 8 621.6 0 8 47 2 621.6 0 2 48 6 621.6 0 6 49 6 621.6 0 6 50 6 621.6 0 6 51 4 621.6 0 4 52 8 621.6 0 8 53 5 621.6 0 5 54 7 621.6 0 7 55 6 621.6 0 6 56 9 621.6 0 9 57 8 621.6 0 8 58 32 621.6 0 32 59 119 621.6 0 119 60 536 621.6 0 536 61 293 621.6 0 293 62 452 621.6 0 452 63 4 621.6 0 4 64 14 621.6 0 14 65 14 621.6 0 14 66 157 621.6 0 157 67 78 621.6 0 78 68 22 621.6 0 22 69 6 621.6 0 6 70 5 621.6 0 5 71 8 621.6 0 8 72 12 621.6 0 12 73 6 621.6 0 6 74 5 621.6 0 5 75 11 621.6 0 11 76 9 621.6 0 9 77 150 621.6 0 150 78 83 621.6 0 83 79 113 621.6 0 113 80 18 621.6 0 18 81 89 621.6 0 89 82 16 621.6 0 16 83 14 621.6 0 14 84 7 621.6 0 7 85 22 621.6 0 22 86 40 621.6 0 40 87 7 621.6 0 7 88 4 621.6 0 4 89 5 621.6 0 5 90 17 621.6 0 17 91 11 621.6 0 11 92 12 621.6 0 12 93 15 621.6 0 15 94 9 621.6 0 9 95 11 621.6 0 11 96 14 621.6 0 14 97 21 621.6 0 21 98 19 621.6 0 19 99 15 621.6 0 15 100 11 621.6 0 11 101 11 621.6 0 11 102 15 621.6 0 15 103 13 621.6 0 13 104 26 621.6 0 26 105 11 621.6 0 11 106 15 621.6 0 15 107 14 621.6 0 14 108 15 621.6 0 15 109 16 621.6 0 16 110 13 621.6 0 13 111 59 621.6 0 59 112 146 621.6 0 146 113 44 621.6 0 44 114 95 621.6 0 95 115 127 621.6 0 127 116 199 621.6 0 199 117 45 621.6 0 45 118 11 621.6 0 11 119 11 621.6 0 11 120 10 621.6 0 10 121 9 621.6 0 9 122 7 621.6 0 7 123 43 621.6 0 43 124 16 621.6 0 16 125 14 621.6 0 14 126 9 621.6 0 9 127 14 621.6 0 14 128 6 621.6 0 6 129 10 621.6 0 10 130 12 621.6 0 12 131 15 621.6 0 15 132 15 621.6 0 15 133 25 621.6 0 25 134 9 621.6 0 9 135 44 621.6 0 44 136 13 621.6 0 13 137 25 621.6 0 25 138 32 621.6 0 32 139 34 621.6 0 34 140 28 621.6 0 28 141 17 621.6 0 17 142 22 621.6 0 22 143 20 621.6 0 20 144 22 621.6 0 22 145 22 621.6 0 22 146 10 621.6 0 10 147 9 621.6 0 9 148 7 621.6 0 7 149 13 621.6 0 13 150 11 621.6 0 11 151 22 621.6 0 22 152 16 621.6 0 16 153 21 621.6 0 21 154 12 621.6 0 12 155 16 621.6 0 16 156 18 621.6 0 18 157 11 621.6 0 11 158 10 621.6 0 10 159 24 621.6 0 24 160 91 621.6 0 91 161 16 621.6 0 16 162 19 621.6 0 19 163 18 621.6 0 18 164 20 621.6 0 20 165 72 621.6 0 72 166 41 621.6 0 41 167 56 621.6 0 56 168 18 621.6 0 18 169 11 621.6 0 11 170 13 621.6 0 13 171 15 621.6 0 15 172 15 621.6 0 15 173 14 621.6 0 14 174 13 621.6 0 13 175 8 621.6 0 8 176 12 621.6 0 12 177 18 621.6 0 18 178 21 621.6 0 21 179 40 621.6 0 40 180 35 621.6 0 35 181 88 621.6 0 88 182 11 621.6 0 11 183 11 621.6 0 11 184 31 621.6 0 31 185 50 621.6 0 50 186 30 621.6 0 30 187 19 621.6 0 19 188 17 621.6 0 17 189 44 621.6 0 44 190 98 621.6 0 98 191 111 621.6 0 111 192 89 621.6 0 89 193 67 621.6 0 67 194 17 621.6 0 17 195 19 621.6 0 19 196 19 621.6 0 19 197 30 621.6 0 30 198 21 621.6 0 21 199 15 621.6 0 15 200 16 621.6 0 16 201 20 621.6 0 20 202 21 621.6 0 21 203 171 621.6 0 171 204 27 621.6 0 27 205 79 621.6 0 79 206 28 621.6 0 28 207 140 621.6 0 140 208 101 621.6 0 101 209 73 621.6 0 73 210 59 621.6 0 59 211 34 621.6 0 34 212 101 621.6 0 101 213 17 621.6 0 17 214 48 621.6 0 48 215 29 621.6 0 29 216 43 621.6 0 43 217 57 621.6 0 57 218 40 621.6 0 40 219 32 621.6 0 32 220 16 621.6 0 16 221 30 621.6 0 30 222 22 621.6 0 22 223 23 621.6 0 23 224 50 621.6 0 50 225 58 621.6 0 58 226 38 621.6 0 38 227 23 621.6 0 23 228 33 621.6 0 33 229 24 621.6 0 24 230 31 621.6 0 31 231 52 621.6 0 52 232 91 621.6 0 91 233 47 621.6 0 47 234 53 621.6 0 53 235 138 621.6 0 138 236 195 621.6 0 195 237 194 621.6 0 194 238 178 621.6 0 178 239 83 621.6 0 83 240 52 621.6 0 52 241 66 621.6 0 66 242 81 621.6 0 81 243 126 621.6 0 126 244 286 621.6 0 286 245 4339 621.6 0 4339 246 1966 621.6 0 1966 247 3696 621.6 0 3696 248 37 621.6 0 37 249 24 621.6 0 24 250 33 621.6 0 33 251 18 621.6 0 18 252 36 621.6 0 36 253 35 621.6 0 35 254 17 621.6 0 17 255 26 621.6 0 26 256 22 621.6 0 22 257 19 621.6 0 19 258 26 621.6 0 26 259 49 621.6 0 49 260 126 621.6 0 126 261 104 621.6 0 104 262 140 621.6 0 140 263 100 621.6 0 100 264 113 621.6 0 113 265 2063 621.6 0 2063 266 204 621.6 0 204 267 132 621.6 0 132 268 32 621.6 0 32 269 19 621.6 0 19 270 109 621.6 0 109 271 94 621.6 0 94 272 86 621.6 0 86 273 23 621.6 0 23 274 25 621.6 0 25 275 15 621.6 0 15 276 60 621.6 0 60 277 49 621.6 0 49 278 58 621.6 0 58 279 37 621.6 0 37 280 46 621.6 0 46 281 29 621.6 0 29 282 30 621.6 0 30 283 31 621.6 0 31 284 39 621.6 0 39 285 43 621.6 0 43 286 51 621.6 0 51 287 55 621.6 0 55 288 33 621.6 0 33 289 25 621.6 0 25 290 21 621.6 0 21 291 28 621.6 0 28 292 23 621.6 0 23 293 37 621.6 0 37 294 30 621.6 0 30 295 48 621.6 0 48 296 24 621.6 0 24 297 28 621.6 0 28 298 52 621.6 0 52 299 39 621.6 0 39 300 54 621.6 0 54 301 28 621.6 0 28 Finished in 2.94 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 59,121 Reads with adapters: 23,059 (39.0%) Reads that were too short: 48 (0.1%) Reads written (passing filters): 23,011 (38.9%) Total basepairs processed: 16,722,105 bp Total written (filtered): 6,349,793 bp (38.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 23059 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 161 0.0 3 0 160 1 17 22739 0.0 3 22379 354 5 1 18 67 0.0 3 8 56 3 19 8 0.0 3 0 0 6 2 20 23 0.0 3 0 0 0 23 24 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 29 1 0.0 3 1 51 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 1 81 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 192 2 0.0 3 1 0 0 1 217 28 0.0 3 0 0 0 28 218 2 0.0 3 0 0 0 2 281 3 0.0 3 0 0 0 3 282 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.80 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 23,011 Reads with adapters: 296 (1.3%) Reads written (passing filters): 23,011 (100.0%) Total basepairs processed: 6,349,793 bp Total written (filtered): 6,348,814 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 296 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 62.8% C: 6.1% G: 28.4% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 214 359.5 0 214 4 80 89.9 0 80 6 1 5.6 0 1 11 1 0.0 1 0 1