This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.56 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 140,928 (5.5%) Reads that were too short: 75,956 (3.0%) Reads written (passing filters): 64,972 (2.6%) Total basepairs processed: 766,381,518 bp Total written (filtered): 14,843,299 bp (1.9%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 140928 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 365 621.6 0 365 7 13837 621.6 0 13837 8 10681 621.6 0 10681 9 279 621.6 0 279 10 235 621.6 0 235 11 5232 621.6 0 5232 12 83 621.6 0 83 13 79 621.6 0 79 14 98 621.6 0 98 15 113 621.6 0 113 16 109 621.6 0 109 17 99 621.6 0 99 18 64 621.6 0 64 19 61 621.6 0 61 20 78 621.6 0 78 21 68 621.6 0 68 22 56 621.6 0 56 23 80 621.6 0 80 24 78 621.6 0 78 25 72 621.6 0 72 26 68 621.6 0 68 27 77 621.6 0 77 28 62 621.6 0 62 29 71 621.6 0 71 30 82 621.6 0 82 31 79 621.6 0 79 32 85 621.6 0 85 33 75 621.6 0 75 34 85 621.6 0 85 35 97 621.6 0 97 36 80 621.6 0 80 37 93 621.6 0 93 38 104 621.6 0 104 39 80 621.6 0 80 40 70 621.6 0 70 41 96 621.6 0 96 42 66 621.6 0 66 43 111 621.6 0 111 44 76 621.6 0 76 45 101 621.6 0 101 46 83 621.6 0 83 47 68 621.6 0 68 48 94 621.6 0 94 49 71 621.6 0 71 50 83 621.6 0 83 51 81 621.6 0 81 52 200 621.6 0 200 53 182 621.6 0 182 54 281 621.6 0 281 55 93 621.6 0 93 56 80 621.6 0 80 57 113 621.6 0 113 58 79 621.6 0 79 59 96 621.6 0 96 60 191 621.6 0 191 61 92 621.6 0 92 62 85 621.6 0 85 63 104 621.6 0 104 64 122 621.6 0 122 65 100 621.6 0 100 66 105 621.6 0 105 67 91 621.6 0 91 68 120 621.6 0 120 69 99 621.6 0 99 70 103 621.6 0 103 71 134 621.6 0 134 72 297 621.6 0 297 73 188 621.6 0 188 74 207 621.6 0 207 75 93 621.6 0 93 76 73 621.6 0 73 77 73 621.6 0 73 78 99 621.6 0 99 79 132 621.6 0 132 80 112 621.6 0 112 81 241 621.6 0 241 82 123 621.6 0 123 83 99 621.6 0 99 84 127 621.6 0 127 85 111 621.6 0 111 86 116 621.6 0 116 87 97 621.6 0 97 88 113 621.6 0 113 89 91 621.6 0 91 90 82 621.6 0 82 91 97 621.6 0 97 92 91 621.6 0 91 93 112 621.6 0 112 94 95 621.6 0 95 95 93 621.6 0 93 96 106 621.6 0 106 97 101 621.6 0 101 98 133 621.6 0 133 99 135 621.6 0 135 100 119 621.6 0 119 101 141 621.6 0 141 102 141 621.6 0 141 103 161 621.6 0 161 104 178 621.6 0 178 105 158 621.6 0 158 106 144 621.6 0 144 107 150 621.6 0 150 108 127 621.6 0 127 109 123 621.6 0 123 110 148 621.6 0 148 111 192 621.6 0 192 112 121 621.6 0 121 113 132 621.6 0 132 114 140 621.6 0 140 115 122 621.6 0 122 116 128 621.6 0 128 117 126 621.6 0 126 118 128 621.6 0 128 119 124 621.6 0 124 120 124 621.6 0 124 121 152 621.6 0 152 122 180 621.6 0 180 123 155 621.6 0 155 124 329 621.6 0 329 125 359 621.6 0 359 126 340 621.6 0 340 127 112 621.6 0 112 128 123 621.6 0 123 129 110 621.6 0 110 130 152 621.6 0 152 131 137 621.6 0 137 132 189 621.6 0 189 133 305 621.6 0 305 134 317 621.6 0 317 135 176 621.6 0 176 136 141 621.6 0 141 137 189 621.6 0 189 138 175 621.6 0 175 139 197 621.6 0 197 140 139 621.6 0 139 141 140 621.6 0 140 142 129 621.6 0 129 143 133 621.6 0 133 144 130 621.6 0 130 145 180 621.6 0 180 146 251 621.6 0 251 147 415 621.6 0 415 148 292 621.6 0 292 149 411 621.6 0 411 150 230 621.6 0 230 151 180 621.6 0 180 152 183 621.6 0 183 153 180 621.6 0 180 154 173 621.6 0 173 155 147 621.6 0 147 156 251 621.6 0 251 157 265 621.6 0 265 158 2209 621.6 0 2209 159 280 621.6 0 280 160 266 621.6 0 266 161 229 621.6 0 229 162 225 621.6 0 225 163 199 621.6 0 199 164 181 621.6 0 181 165 161 621.6 0 161 166 252 621.6 0 252 167 259 621.6 0 259 168 354 621.6 0 354 169 711 621.6 0 711 170 1024 621.6 0 1024 171 1455 621.6 0 1455 172 786 621.6 0 786 173 275 621.6 0 275 174 190 621.6 0 190 175 180 621.6 0 180 176 152 621.6 0 152 177 145 621.6 0 145 178 161 621.6 0 161 179 200 621.6 0 200 180 267 621.6 0 267 181 290 621.6 0 290 182 295 621.6 0 295 183 241 621.6 0 241 184 229 621.6 0 229 185 234 621.6 0 234 186 166 621.6 0 166 187 380 621.6 0 380 188 217 621.6 0 217 189 306 621.6 0 306 190 146 621.6 0 146 191 140 621.6 0 140 192 172 621.6 0 172 193 215 621.6 0 215 194 214 621.6 0 214 195 190 621.6 0 190 196 159 621.6 0 159 197 137 621.6 0 137 198 159 621.6 0 159 199 180 621.6 0 180 200 178 621.6 0 178 201 157 621.6 0 157 202 171 621.6 0 171 203 294 621.6 0 294 204 239 621.6 0 239 205 256 621.6 0 256 206 159 621.6 0 159 207 138 621.6 0 138 208 143 621.6 0 143 209 204 621.6 0 204 210 168 621.6 0 168 211 184 621.6 0 184 212 160 621.6 0 160 213 228 621.6 0 228 214 711 621.6 0 711 215 879 621.6 0 879 216 987 621.6 0 987 217 595 621.6 0 595 218 388 621.6 0 388 219 405 621.6 0 405 220 219 621.6 0 219 221 201 621.6 0 201 222 239 621.6 0 239 223 308 621.6 0 308 224 405 621.6 0 405 225 885 621.6 0 885 226 3956 621.6 0 3956 227 8237 621.6 0 8237 228 6957 621.6 0 6957 229 5832 621.6 0 5832 230 1092 621.6 0 1092 231 1137 621.6 0 1137 232 900 621.6 0 900 233 479 621.6 0 479 234 160 621.6 0 160 235 145 621.6 0 145 236 182 621.6 0 182 237 303 621.6 0 303 238 498 621.6 0 498 239 530 621.6 0 530 240 333 621.6 0 333 241 149 621.6 0 149 242 132 621.6 0 132 243 155 621.6 0 155 244 178 621.6 0 178 245 179 621.6 0 179 246 180 621.6 0 180 247 181 621.6 0 181 248 225 621.6 0 225 249 276 621.6 0 276 250 234 621.6 0 234 251 159 621.6 0 159 252 152 621.6 0 152 253 155 621.6 0 155 254 196 621.6 0 196 255 234 621.6 0 234 256 302 621.6 0 302 257 369 621.6 0 369 258 265 621.6 0 265 259 379 621.6 0 379 260 274 621.6 0 274 261 359 621.6 0 359 262 346 621.6 0 346 263 477 621.6 0 477 264 2230 621.6 0 2230 265 3338 621.6 0 3338 266 4676 621.6 0 4676 267 2681 621.6 0 2681 268 2089 621.6 0 2089 269 590 621.6 0 590 270 483 621.6 0 483 271 368 621.6 0 368 272 334 621.6 0 334 273 523 621.6 0 523 274 597 621.6 0 597 275 604 621.6 0 604 276 538 621.6 0 538 277 304 621.6 0 304 278 312 621.6 0 312 279 496 621.6 0 496 280 517 621.6 0 517 281 500 621.6 0 500 282 245 621.6 0 245 283 222 621.6 0 222 284 259 621.6 0 259 285 313 621.6 0 313 286 433 621.6 0 433 287 465 621.6 0 465 288 287 621.6 0 287 289 314 621.6 0 314 290 313 621.6 0 313 291 1707 621.6 0 1707 292 1570 621.6 0 1570 293 2039 621.6 0 2039 294 358 621.6 0 358 295 187 621.6 0 187 296 170 621.6 0 170 297 135 621.6 0 135 298 151 621.6 0 151 299 162 621.6 0 162 300 178 621.6 0 178 301 405 621.6 0 405 Finished in 3.13 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 64,972 Reads with adapters: 10,955 (16.9%) Reads that were too short: 29 (0.0%) Reads written (passing filters): 10,926 (16.8%) Total basepairs processed: 14,843,299 bp Total written (filtered): 2,978,262 bp (20.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10955 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 8 0.0 3 0 0 4 2 2 19 173 0.0 3 0 143 13 11 6 20 10605 0.0 4 9755 648 144 41 17 21 36 0.0 4 6 24 3 3 22 6 0.0 4 1 1 1 2 1 23 53 0.0 4 0 0 0 39 14 30 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 1 50 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 1 82 3 0.0 4 0 0 0 0 3 94 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 1 125 31 0.0 4 0 0 0 0 31 150 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 157 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 1 200 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 226 2 0.0 4 0 0 0 0 2 233 2 0.0 4 0 0 0 0 2 234 3 0.0 4 0 0 0 1 2 237 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 0 2 275 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.40 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 10,926 Reads with adapters: 2,136 (19.5%) Reads written (passing filters): 10,926 (100.0%) Total basepairs processed: 2,978,262 bp Total written (filtered): 2,952,844 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2136 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.4% C: 9.3% G: 84.6% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 15 170.7 0 15 4 4 42.7 0 4 8 1 0.2 0 1 9 20 0.0 0 0 20 10 527 0.0 1 34 493 11 1541 0.0 1 52 1489 12 11 0.0 1 0 11 14 2 0.0 1 0 2 19 1 0.0 1 0 1 73 1 0.0 1 1 151 1 0.0 1 0 1 209 11 0.0 1 7 4 246 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.