This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.77 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 101,413 (4.0%) Reads that were too short: 40,587 (1.6%) Reads written (passing filters): 60,826 (2.4%) Total basepairs processed: 766,381,518 bp Total written (filtered): 14,398,061 bp (1.9%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 101413 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 289 621.6 0 289 7 11342 621.6 0 11342 8 14313 621.6 0 14313 9 154 621.6 0 154 10 198 621.6 0 198 11 5084 621.6 0 5084 12 85 621.6 0 85 13 89 621.6 0 89 14 95 621.6 0 95 15 83 621.6 0 83 16 87 621.6 0 87 17 107 621.6 0 107 18 83 621.6 0 83 19 76 621.6 0 76 20 85 621.6 0 85 21 69 621.6 0 69 22 82 621.6 0 82 23 97 621.6 0 97 24 95 621.6 0 95 25 99 621.6 0 99 26 79 621.6 0 79 27 79 621.6 0 79 28 68 621.6 0 68 29 77 621.6 0 77 30 84 621.6 0 84 31 104 621.6 0 104 32 92 621.6 0 92 33 74 621.6 0 74 34 93 621.6 0 93 35 115 621.6 0 115 36 101 621.6 0 101 37 89 621.6 0 89 38 108 621.6 0 108 39 91 621.6 0 91 40 71 621.6 0 71 41 97 621.6 0 97 42 64 621.6 0 64 43 62 621.6 0 62 44 91 621.6 0 91 45 104 621.6 0 104 46 79 621.6 0 79 47 84 621.6 0 84 48 83 621.6 0 83 49 75 621.6 0 75 50 90 621.6 0 90 51 85 621.6 0 85 52 156 621.6 0 156 53 103 621.6 0 103 54 119 621.6 0 119 55 67 621.6 0 67 56 99 621.6 0 99 57 79 621.6 0 79 58 77 621.6 0 77 59 102 621.6 0 102 60 179 621.6 0 179 61 110 621.6 0 110 62 68 621.6 0 68 63 92 621.6 0 92 64 119 621.6 0 119 65 104 621.6 0 104 66 130 621.6 0 130 67 76 621.6 0 76 68 119 621.6 0 119 69 122 621.6 0 122 70 117 621.6 0 117 71 111 621.6 0 111 72 140 621.6 0 140 73 124 621.6 0 124 74 132 621.6 0 132 75 104 621.6 0 104 76 95 621.6 0 95 77 105 621.6 0 105 78 121 621.6 0 121 79 109 621.6 0 109 80 159 621.6 0 159 81 290 621.6 0 290 82 156 621.6 0 156 83 148 621.6 0 148 84 179 621.6 0 179 85 161 621.6 0 161 86 168 621.6 0 168 87 137 621.6 0 137 88 157 621.6 0 157 89 99 621.6 0 99 90 100 621.6 0 100 91 131 621.6 0 131 92 121 621.6 0 121 93 121 621.6 0 121 94 97 621.6 0 97 95 104 621.6 0 104 96 129 621.6 0 129 97 93 621.6 0 93 98 168 621.6 0 168 99 186 621.6 0 186 100 130 621.6 0 130 101 157 621.6 0 157 102 140 621.6 0 140 103 187 621.6 0 187 104 124 621.6 0 124 105 128 621.6 0 128 106 122 621.6 0 122 107 164 621.6 0 164 108 112 621.6 0 112 109 136 621.6 0 136 110 151 621.6 0 151 111 128 621.6 0 128 112 122 621.6 0 122 113 118 621.6 0 118 114 118 621.6 0 118 115 117 621.6 0 117 116 158 621.6 0 158 117 126 621.6 0 126 118 118 621.6 0 118 119 118 621.6 0 118 120 111 621.6 0 111 121 129 621.6 0 129 122 143 621.6 0 143 123 136 621.6 0 136 124 211 621.6 0 211 125 195 621.6 0 195 126 179 621.6 0 179 127 128 621.6 0 128 128 125 621.6 0 125 129 117 621.6 0 117 130 136 621.6 0 136 131 127 621.6 0 127 132 174 621.6 0 174 133 147 621.6 0 147 134 246 621.6 0 246 135 136 621.6 0 136 136 149 621.6 0 149 137 141 621.6 0 141 138 122 621.6 0 122 139 162 621.6 0 162 140 132 621.6 0 132 141 111 621.6 0 111 142 109 621.6 0 109 143 130 621.6 0 130 144 116 621.6 0 116 145 143 621.6 0 143 146 170 621.6 0 170 147 339 621.6 0 339 148 183 621.6 0 183 149 285 621.6 0 285 150 136 621.6 0 136 151 121 621.6 0 121 152 125 621.6 0 125 153 163 621.6 0 163 154 175 621.6 0 175 155 153 621.6 0 153 156 176 621.6 0 176 157 219 621.6 0 219 158 3032 621.6 0 3032 159 162 621.6 0 162 160 136 621.6 0 136 161 128 621.6 0 128 162 159 621.6 0 159 163 126 621.6 0 126 164 138 621.6 0 138 165 135 621.6 0 135 166 155 621.6 0 155 167 152 621.6 0 152 168 151 621.6 0 151 169 363 621.6 0 363 170 588 621.6 0 588 171 834 621.6 0 834 172 451 621.6 0 451 173 147 621.6 0 147 174 156 621.6 0 156 175 144 621.6 0 144 176 127 621.6 0 127 177 117 621.6 0 117 178 145 621.6 0 145 179 168 621.6 0 168 180 157 621.6 0 157 181 157 621.6 0 157 182 151 621.6 0 151 183 163 621.6 0 163 184 173 621.6 0 173 185 171 621.6 0 171 186 114 621.6 0 114 187 196 621.6 0 196 188 227 621.6 0 227 189 175 621.6 0 175 190 158 621.6 0 158 191 131 621.6 0 131 192 196 621.6 0 196 193 178 621.6 0 178 194 194 621.6 0 194 195 169 621.6 0 169 196 144 621.6 0 144 197 140 621.6 0 140 198 163 621.6 0 163 199 158 621.6 0 158 200 151 621.6 0 151 201 138 621.6 0 138 202 123 621.6 0 123 203 199 621.6 0 199 204 178 621.6 0 178 205 191 621.6 0 191 206 141 621.6 0 141 207 133 621.6 0 133 208 131 621.6 0 131 209 134 621.6 0 134 210 126 621.6 0 126 211 137 621.6 0 137 212 147 621.6 0 147 213 164 621.6 0 164 214 327 621.6 0 327 215 484 621.6 0 484 216 405 621.6 0 405 217 262 621.6 0 262 218 203 621.6 0 203 219 181 621.6 0 181 220 136 621.6 0 136 221 139 621.6 0 139 222 160 621.6 0 160 223 204 621.6 0 204 224 235 621.6 0 235 225 460 621.6 0 460 226 1917 621.6 0 1917 227 5127 621.6 0 5127 228 3870 621.6 0 3870 229 4017 621.6 0 4017 230 684 621.6 0 684 231 902 621.6 0 902 232 679 621.6 0 679 233 467 621.6 0 467 234 124 621.6 0 124 235 113 621.6 0 113 236 132 621.6 0 132 237 168 621.6 0 168 238 216 621.6 0 216 239 213 621.6 0 213 240 188 621.6 0 188 241 113 621.6 0 113 242 114 621.6 0 114 243 96 621.6 0 96 244 136 621.6 0 136 245 134 621.6 0 134 246 154 621.6 0 154 247 146 621.6 0 146 248 194 621.6 0 194 249 193 621.6 0 193 250 158 621.6 0 158 251 135 621.6 0 135 252 143 621.6 0 143 253 124 621.6 0 124 254 131 621.6 0 131 255 127 621.6 0 127 256 146 621.6 0 146 257 165 621.6 0 165 258 152 621.6 0 152 259 164 621.6 0 164 260 178 621.6 0 178 261 238 621.6 0 238 262 207 621.6 0 207 263 242 621.6 0 242 264 789 621.6 0 789 265 1078 621.6 0 1078 266 1598 621.6 0 1598 267 915 621.6 0 915 268 867 621.6 0 867 269 257 621.6 0 257 270 253 621.6 0 253 271 212 621.6 0 212 272 166 621.6 0 166 273 221 621.6 0 221 274 273 621.6 0 273 275 318 621.6 0 318 276 322 621.6 0 322 277 164 621.6 0 164 278 183 621.6 0 183 279 226 621.6 0 226 280 257 621.6 0 257 281 245 621.6 0 245 282 219 621.6 0 219 283 164 621.6 0 164 284 206 621.6 0 206 285 158 621.6 0 158 286 185 621.6 0 185 287 221 621.6 0 221 288 183 621.6 0 183 289 184 621.6 0 184 290 187 621.6 0 187 291 678 621.6 0 678 292 606 621.6 0 606 293 641 621.6 0 641 294 214 621.6 0 214 295 111 621.6 0 111 296 127 621.6 0 127 297 128 621.6 0 128 298 128 621.6 0 128 299 143 621.6 0 143 300 150 621.6 0 150 301 203 621.6 0 203 Finished in 2.65 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 60,826 Reads with adapters: 11,094 (18.2%) Reads that were too short: 32 (0.1%) Reads written (passing filters): 11,062 (18.2%) Total basepairs processed: 14,398,061 bp Total written (filtered): 3,056,482 bp (21.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11094 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 2 0.0 3 0 0 2 16 78 0.0 3 0 78 17 10879 0.0 3 10651 218 9 1 18 30 0.0 3 0 30 19 5 0.0 3 0 0 4 1 20 21 0.0 3 1 0 0 20 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 26 1 0.0 3 1 32 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 67 3 0.0 3 0 0 0 3 75 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 92 1 0.0 3 0 1 102 1 0.0 3 1 105 1 0.0 3 1 106 1 0.0 3 0 0 0 1 116 2 0.0 3 0 1 0 1 118 1 0.0 3 0 1 119 2 0.0 3 1 1 131 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 157 4 0.0 3 0 0 0 4 160 1 0.0 3 0 0 0 1 162 4 0.0 3 0 0 0 4 165 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 168 3 0.0 3 0 0 0 3 170 1 0.0 3 1 179 1 0.0 3 0 0 0 1 182 12 0.0 3 0 0 0 12 183 6 0.0 3 0 0 0 6 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.39 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 11,062 Reads with adapters: 5 (0.0%) Reads written (passing filters): 11,062 (100.0%) Total basepairs processed: 3,056,482 bp Total written (filtered): 3,056,466 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.0% C: 20.0% G: 40.0% T: 20.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 172.8 0 4 4 1 43.2 0 1