This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.94 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 36,060 (1.4%) Reads that were too short: 7,863 (0.3%) Reads written (passing filters): 28,197 (1.1%) Total basepairs processed: 766,381,518 bp Total written (filtered): 6,248,305 bp (0.8%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 36060 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 332 621.6 0 332 7 4501 621.6 0 4501 8 149 621.6 0 149 9 4405 621.6 0 4405 10 11 621.6 0 11 11 9 621.6 0 9 12 6 621.6 0 6 13 10 621.6 0 10 14 3 621.6 0 3 15 11 621.6 0 11 16 2 621.6 0 2 17 1 621.6 0 1 18 2 621.6 0 2 19 4 621.6 0 4 20 1 621.6 0 1 21 4 621.6 0 4 22 10 621.6 0 10 23 19 621.6 0 19 24 16 621.6 0 16 25 92 621.6 0 92 26 25 621.6 0 25 27 24 621.6 0 24 28 9 621.6 0 9 29 5 621.6 0 5 30 6 621.6 0 6 31 3 621.6 0 3 32 12 621.6 0 12 33 29 621.6 0 29 34 85 621.6 0 85 35 176 621.6 0 176 36 4 621.6 0 4 37 6 621.6 0 6 38 4 621.6 0 4 39 5 621.6 0 5 40 12 621.6 0 12 41 3 621.6 0 3 42 4 621.6 0 4 43 4 621.6 0 4 44 4 621.6 0 4 45 1 621.6 0 1 46 9 621.6 0 9 47 6 621.6 0 6 48 4 621.6 0 4 49 7 621.6 0 7 50 3 621.6 0 3 51 5 621.6 0 5 52 6 621.6 0 6 53 6 621.6 0 6 54 1 621.6 0 1 55 3 621.6 0 3 56 5 621.6 0 5 57 7 621.6 0 7 58 6 621.6 0 6 59 5 621.6 0 5 60 11 621.6 0 11 61 3 621.6 0 3 62 8 621.6 0 8 63 6 621.6 0 6 64 10 621.6 0 10 65 7 621.6 0 7 66 11 621.6 0 11 67 3 621.6 0 3 68 8 621.6 0 8 69 2 621.6 0 2 71 3 621.6 0 3 72 1 621.6 0 1 73 10 621.6 0 10 74 9 621.6 0 9 75 19 621.6 0 19 76 66 621.6 0 66 77 31 621.6 0 31 78 42 621.6 0 42 79 62 621.6 0 62 80 22 621.6 0 22 81 43 621.6 0 43 82 29 621.6 0 29 83 26 621.6 0 26 84 7 621.6 0 7 85 18 621.6 0 18 86 33 621.6 0 33 87 25 621.6 0 25 88 30 621.6 0 30 89 56 621.6 0 56 90 19 621.6 0 19 91 9 621.6 0 9 92 16 621.6 0 16 93 21 621.6 0 21 94 24 621.6 0 24 95 62 621.6 0 62 96 37 621.6 0 37 97 106 621.6 0 106 98 145 621.6 0 145 99 1227 621.6 0 1227 100 472 621.6 0 472 101 868 621.6 0 868 102 83 621.6 0 83 103 35 621.6 0 35 104 35 621.6 0 35 105 34 621.6 0 34 106 60 621.6 0 60 107 42 621.6 0 42 108 56 621.6 0 56 109 96 621.6 0 96 110 127 621.6 0 127 111 1615 621.6 0 1615 112 1251 621.6 0 1251 113 3719 621.6 0 3719 114 371 621.6 0 371 115 2698 621.6 0 2698 116 19 621.6 0 19 117 8 621.6 0 8 118 13 621.6 0 13 119 21 621.6 0 21 120 20 621.6 0 20 121 21 621.6 0 21 122 10 621.6 0 10 123 11 621.6 0 11 124 10 621.6 0 10 125 17 621.6 0 17 126 16 621.6 0 16 127 34 621.6 0 34 128 18 621.6 0 18 129 55 621.6 0 55 130 82 621.6 0 82 131 128 621.6 0 128 132 81 621.6 0 81 133 63 621.6 0 63 134 50 621.6 0 50 135 120 621.6 0 120 136 82 621.6 0 82 137 231 621.6 0 231 138 26 621.6 0 26 139 28 621.6 0 28 140 25 621.6 0 25 141 30 621.6 0 30 142 116 621.6 0 116 143 406 621.6 0 406 144 455 621.6 0 455 145 603 621.6 0 603 146 53 621.6 0 53 147 61 621.6 0 61 148 24 621.6 0 24 149 32 621.6 0 32 150 31 621.6 0 31 151 33 621.6 0 33 152 17 621.6 0 17 153 22 621.6 0 22 154 18 621.6 0 18 155 15 621.6 0 15 156 21 621.6 0 21 157 19 621.6 0 19 158 31 621.6 0 31 159 29 621.6 0 29 160 44 621.6 0 44 161 23 621.6 0 23 162 48 621.6 0 48 163 38 621.6 0 38 164 31 621.6 0 31 165 33 621.6 0 33 166 43 621.6 0 43 167 27 621.6 0 27 168 25 621.6 0 25 169 22 621.6 0 22 170 17 621.6 0 17 171 21 621.6 0 21 172 21 621.6 0 21 173 15 621.6 0 15 174 17 621.6 0 17 175 31 621.6 0 31 176 24 621.6 0 24 177 41 621.6 0 41 178 40 621.6 0 40 179 23 621.6 0 23 180 27 621.6 0 27 181 24 621.6 0 24 182 24 621.6 0 24 183 14 621.6 0 14 184 28 621.6 0 28 185 21 621.6 0 21 186 19 621.6 0 19 187 27 621.6 0 27 188 24 621.6 0 24 189 20 621.6 0 20 190 23 621.6 0 23 191 14 621.6 0 14 192 15 621.6 0 15 193 32 621.6 0 32 194 20 621.6 0 20 195 22 621.6 0 22 196 24 621.6 0 24 197 19 621.6 0 19 198 20 621.6 0 20 199 32 621.6 0 32 200 25 621.6 0 25 201 23 621.6 0 23 202 49 621.6 0 49 203 46 621.6 0 46 204 40 621.6 0 40 205 21 621.6 0 21 206 24 621.6 0 24 207 27 621.6 0 27 208 28 621.6 0 28 209 40 621.6 0 40 210 38 621.6 0 38 211 28 621.6 0 28 212 26 621.6 0 26 213 27 621.6 0 27 214 28 621.6 0 28 215 39 621.6 0 39 216 42 621.6 0 42 217 37 621.6 0 37 218 35 621.6 0 35 219 38 621.6 0 38 220 39 621.6 0 39 221 41 621.6 0 41 222 53 621.6 0 53 223 54 621.6 0 54 224 66 621.6 0 66 225 42 621.6 0 42 226 97 621.6 0 97 227 38 621.6 0 38 228 27 621.6 0 27 229 31 621.6 0 31 230 40 621.6 0 40 231 25 621.6 0 25 232 36 621.6 0 36 233 45 621.6 0 45 234 60 621.6 0 60 235 43 621.6 0 43 236 45 621.6 0 45 237 33 621.6 0 33 238 41 621.6 0 41 239 71 621.6 0 71 240 45 621.6 0 45 241 85 621.6 0 85 242 98 621.6 0 98 243 94 621.6 0 94 244 53 621.6 0 53 245 43 621.6 0 43 246 31 621.6 0 31 247 28 621.6 0 28 248 39 621.6 0 39 249 30 621.6 0 30 250 40 621.6 0 40 251 29 621.6 0 29 252 92 621.6 0 92 253 42 621.6 0 42 254 63 621.6 0 63 255 49 621.6 0 49 256 75 621.6 0 75 257 79 621.6 0 79 258 63 621.6 0 63 259 39 621.6 0 39 260 53 621.6 0 53 261 54 621.6 0 54 262 130 621.6 0 130 263 152 621.6 0 152 264 177 621.6 0 177 265 92 621.6 0 92 266 41 621.6 0 41 267 35 621.6 0 35 268 39 621.6 0 39 269 43 621.6 0 43 270 57 621.6 0 57 271 66 621.6 0 66 272 60 621.6 0 60 273 76 621.6 0 76 274 38 621.6 0 38 275 103 621.6 0 103 276 118 621.6 0 118 277 167 621.6 0 167 278 111 621.6 0 111 279 53 621.6 0 53 280 54 621.6 0 54 281 71 621.6 0 71 282 199 621.6 0 199 283 389 621.6 0 389 284 678 621.6 0 678 285 363 621.6 0 363 286 47 621.6 0 47 287 50 621.6 0 50 288 49 621.6 0 49 289 55 621.6 0 55 290 85 621.6 0 85 291 156 621.6 0 156 292 200 621.6 0 200 293 182 621.6 0 182 294 296 621.6 0 296 295 225 621.6 0 225 296 135 621.6 0 135 297 62 621.6 0 62 298 75 621.6 0 75 299 58 621.6 0 58 300 64 621.6 0 64 301 48 621.6 0 48 Finished in 1.32 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 28,197 Reads with adapters: 4,745 (16.8%) Reads that were too short: 33 (0.1%) Reads written (passing filters): 4,712 (16.7%) Total basepairs processed: 6,248,305 bp Total written (filtered): 1,288,955 bp (20.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4745 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 3 0.0 3 0 0 3 19 49 0.0 3 0 36 6 6 1 20 4563 0.0 4 4218 271 54 14 6 21 46 0.0 4 32 10 3 1 22 1 0.0 4 0 0 1 23 27 0.0 4 4 3 1 13 6 35 19 0.0 4 0 0 0 0 19 41 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 100 2 0.0 4 0 0 0 0 2 115 1 0.0 4 0 0 0 0 1 122 6 0.0 4 0 0 0 0 6 123 6 0.0 4 0 0 0 1 5 124 3 0.0 4 0 0 0 0 3 127 2 0.0 4 0 0 0 0 2 128 3 0.0 4 0 0 0 0 3 134 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 1 171 2 0.0 4 0 0 0 0 2 190 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.16 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 4,712 Reads with adapters: 313 (6.6%) Reads written (passing filters): 4,712 (100.0%) Total basepairs processed: 1,288,955 bp Total written (filtered): 1,284,581 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 313 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.7% C: 11.2% G: 78.9% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 33 73.6 0 33 4 4 18.4 0 4 9 1 0.0 0 1 10 8 0.0 1 0 8 11 178 0.0 1 5 173 12 80 0.0 1 2 78 13 2 0.0 1 0 2 72 1 0.0 1 0 1 152 2 0.0 1 0 2 153 1 0.0 1 0 1 210 1 0.0 1 1 225 1 0.0 1 1 262 1 0.0 1 1