This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.36 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 30,682 (1.2%) Reads that were too short: 5,463 (0.2%) Reads written (passing filters): 25,219 (1.0%) Total basepairs processed: 766,381,518 bp Total written (filtered): 5,694,407 bp (0.7%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 30682 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 335 621.6 0 335 7 5415 621.6 0 5415 8 123 621.6 0 123 9 4019 621.6 0 4019 10 1 621.6 0 1 11 1 621.6 0 1 12 3 621.6 0 3 14 1 621.6 0 1 15 1 621.6 0 1 16 1 621.6 0 1 17 1 621.6 0 1 18 1 621.6 0 1 19 1 621.6 0 1 21 5 621.6 0 5 22 1 621.6 0 1 23 1 621.6 0 1 24 1 621.6 0 1 25 1 621.6 0 1 27 1 621.6 0 1 28 3 621.6 0 3 29 2 621.6 0 2 30 3 621.6 0 3 32 1 621.6 0 1 33 1 621.6 0 1 34 2 621.6 0 2 35 5 621.6 0 5 36 1 621.6 0 1 37 4 621.6 0 4 38 1 621.6 0 1 39 4 621.6 0 4 40 11 621.6 0 11 41 15 621.6 0 15 42 4 621.6 0 4 43 3 621.6 0 3 44 1 621.6 0 1 45 2 621.6 0 2 46 2 621.6 0 2 47 3 621.6 0 3 48 1 621.6 0 1 49 4 621.6 0 4 50 8 621.6 0 8 51 3 621.6 0 3 52 3 621.6 0 3 53 2 621.6 0 2 54 2 621.6 0 2 56 2 621.6 0 2 57 1 621.6 0 1 58 3 621.6 0 3 60 1 621.6 0 1 61 2 621.6 0 2 62 6 621.6 0 6 64 3 621.6 0 3 65 1 621.6 0 1 66 2 621.6 0 2 67 1 621.6 0 1 68 10 621.6 0 10 69 4 621.6 0 4 70 3 621.6 0 3 71 3 621.6 0 3 72 4 621.6 0 4 73 4 621.6 0 4 74 4 621.6 0 4 75 6 621.6 0 6 76 2 621.6 0 2 77 6 621.6 0 6 78 12 621.6 0 12 79 8 621.6 0 8 80 1 621.6 0 1 81 13 621.6 0 13 82 10 621.6 0 10 83 7 621.6 0 7 84 8 621.6 0 8 85 7 621.6 0 7 86 20 621.6 0 20 87 20 621.6 0 20 88 16 621.6 0 16 89 9 621.6 0 9 90 15 621.6 0 15 91 14 621.6 0 14 92 21 621.6 0 21 93 23 621.6 0 23 94 25 621.6 0 25 95 17 621.6 0 17 96 34 621.6 0 34 97 46 621.6 0 46 98 70 621.6 0 70 99 457 621.6 0 457 100 226 621.6 0 226 101 230 621.6 0 230 102 48 621.6 0 48 103 29 621.6 0 29 104 48 621.6 0 48 105 34 621.6 0 34 106 50 621.6 0 50 107 46 621.6 0 46 108 57 621.6 0 57 109 69 621.6 0 69 110 125 621.6 0 125 111 1690 621.6 0 1690 112 1231 621.6 0 1231 113 3885 621.6 0 3885 114 379 621.6 0 379 115 2756 621.6 0 2756 116 13 621.6 0 13 117 6 621.6 0 6 118 11 621.6 0 11 119 17 621.6 0 17 120 9 621.6 0 9 121 15 621.6 0 15 122 6 621.6 0 6 123 7 621.6 0 7 124 7 621.6 0 7 125 14 621.6 0 14 126 9 621.6 0 9 127 11 621.6 0 11 128 14 621.6 0 14 129 43 621.6 0 43 130 44 621.6 0 44 131 85 621.6 0 85 132 39 621.6 0 39 133 32 621.6 0 32 134 16 621.6 0 16 135 81 621.6 0 81 136 61 621.6 0 61 137 225 621.6 0 225 138 16 621.6 0 16 139 25 621.6 0 25 140 27 621.6 0 27 141 23 621.6 0 23 142 106 621.6 0 106 143 474 621.6 0 474 144 483 621.6 0 483 145 593 621.6 0 593 146 17 621.6 0 17 147 27 621.6 0 27 148 9 621.6 0 9 149 16 621.6 0 16 150 24 621.6 0 24 151 13 621.6 0 13 152 12 621.6 0 12 153 15 621.6 0 15 154 17 621.6 0 17 155 10 621.6 0 10 156 11 621.6 0 11 157 17 621.6 0 17 158 14 621.6 0 14 159 18 621.6 0 18 160 16 621.6 0 16 161 15 621.6 0 15 162 22 621.6 0 22 163 19 621.6 0 19 164 14 621.6 0 14 165 15 621.6 0 15 166 13 621.6 0 13 167 17 621.6 0 17 168 16 621.6 0 16 169 13 621.6 0 13 170 12 621.6 0 12 171 12 621.6 0 12 172 13 621.6 0 13 173 11 621.6 0 11 174 15 621.6 0 15 175 9 621.6 0 9 176 11 621.6 0 11 177 22 621.6 0 22 178 26 621.6 0 26 179 17 621.6 0 17 180 24 621.6 0 24 181 17 621.6 0 17 182 11 621.6 0 11 183 6 621.6 0 6 184 14 621.6 0 14 185 20 621.6 0 20 186 23 621.6 0 23 187 16 621.6 0 16 188 11 621.6 0 11 189 27 621.6 0 27 190 15 621.6 0 15 191 15 621.6 0 15 192 10 621.6 0 10 193 19 621.6 0 19 194 18 621.6 0 18 195 11 621.6 0 11 196 17 621.6 0 17 197 22 621.6 0 22 198 13 621.6 0 13 199 15 621.6 0 15 200 10 621.6 0 10 201 17 621.6 0 17 202 22 621.6 0 22 203 23 621.6 0 23 204 13 621.6 0 13 205 15 621.6 0 15 206 20 621.6 0 20 207 9 621.6 0 9 208 27 621.6 0 27 209 17 621.6 0 17 210 19 621.6 0 19 211 24 621.6 0 24 212 18 621.6 0 18 213 16 621.6 0 16 214 19 621.6 0 19 215 18 621.6 0 18 216 21 621.6 0 21 217 28 621.6 0 28 218 18 621.6 0 18 219 22 621.6 0 22 220 20 621.6 0 20 221 27 621.6 0 27 222 40 621.6 0 40 223 36 621.6 0 36 224 40 621.6 0 40 225 31 621.6 0 31 226 93 621.6 0 93 227 12 621.6 0 12 228 11 621.6 0 11 229 22 621.6 0 22 230 25 621.6 0 25 231 18 621.6 0 18 232 23 621.6 0 23 233 24 621.6 0 24 234 39 621.6 0 39 235 21 621.6 0 21 236 22 621.6 0 22 237 19 621.6 0 19 238 27 621.6 0 27 239 31 621.6 0 31 240 19 621.6 0 19 241 49 621.6 0 49 242 60 621.6 0 60 243 51 621.6 0 51 244 36 621.6 0 36 245 29 621.6 0 29 246 24 621.6 0 24 247 22 621.6 0 22 248 25 621.6 0 25 249 23 621.6 0 23 250 24 621.6 0 24 251 22 621.6 0 22 252 42 621.6 0 42 253 29 621.6 0 29 254 35 621.6 0 35 255 35 621.6 0 35 256 39 621.6 0 39 257 46 621.6 0 46 258 34 621.6 0 34 259 41 621.6 0 41 260 35 621.6 0 35 261 34 621.6 0 34 262 76 621.6 0 76 263 59 621.6 0 59 264 73 621.6 0 73 265 42 621.6 0 42 266 22 621.6 0 22 267 23 621.6 0 23 268 37 621.6 0 37 269 34 621.6 0 34 270 28 621.6 0 28 271 25 621.6 0 25 272 32 621.6 0 32 273 40 621.6 0 40 274 22 621.6 0 22 275 57 621.6 0 57 276 43 621.6 0 43 277 118 621.6 0 118 278 56 621.6 0 56 279 33 621.6 0 33 280 29 621.6 0 29 281 54 621.6 0 54 282 149 621.6 0 149 283 266 621.6 0 266 284 296 621.6 0 296 285 183 621.6 0 183 286 31 621.6 0 31 287 41 621.6 0 41 288 39 621.6 0 39 289 60 621.6 0 60 290 125 621.6 0 125 291 182 621.6 0 182 292 276 621.6 0 276 293 147 621.6 0 147 294 393 621.6 0 393 295 257 621.6 0 257 296 176 621.6 0 176 297 74 621.6 0 74 298 47 621.6 0 47 299 58 621.6 0 58 300 40 621.6 0 40 301 36 621.6 0 36 Finished in 1.08 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 25,219 Reads with adapters: 4,229 (16.8%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 4,219 (16.7%) Total basepairs processed: 5,694,407 bp Total written (filtered): 1,155,856 bp (20.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4229 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 33 0.0 3 0 33 17 4137 0.0 3 4058 77 2 18 8 0.0 3 5 3 19 4 0.0 3 0 0 2 2 20 3 0.0 3 0 0 0 3 26 2 0.0 3 0 0 0 2 29 10 0.0 3 0 0 0 10 32 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 72 3 0.0 3 0 0 0 3 73 3 0.0 3 0 0 0 3 74 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 92 4 0.0 3 0 0 0 4 97 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 139 1 0.0 3 1 141 1 0.0 3 0 0 0 1 144 3 0.0 3 0 0 0 3 184 1 0.0 3 0 0 0 1 193 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 4,219 Reads with adapters: 80 (1.9%) Reads written (passing filters): 4,219 (100.0%) Total basepairs processed: 1,155,856 bp Total written (filtered): 1,155,609 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 80 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 78.8% C: 7.5% G: 11.2% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 73 65.9 0 73 4 7 16.5 0 7