This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.05 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 53,135 (2.1%) Reads that were too short: 17,563 (0.7%) Reads written (passing filters): 35,572 (1.4%) Total basepairs processed: 766,381,518 bp Total written (filtered): 7,315,271 bp (1.0%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 53135 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1038 621.6 0 1038 7 3511 621.6 0 3511 8 161 621.6 0 161 9 3228 621.6 0 3228 10 46 621.6 0 46 11 44 621.6 0 44 12 65 621.6 0 65 13 83 621.6 0 83 14 62 621.6 0 62 15 63 621.6 0 63 16 62 621.6 0 62 17 69 621.6 0 69 18 75 621.6 0 75 19 39 621.6 0 39 20 36 621.6 0 36 21 37 621.6 0 37 22 51 621.6 0 51 23 44 621.6 0 44 24 38 621.6 0 38 25 48 621.6 0 48 26 47 621.6 0 47 27 62 621.6 0 62 28 77 621.6 0 77 29 84 621.6 0 84 30 58 621.6 0 58 31 64 621.6 0 64 32 57 621.6 0 57 33 128 621.6 0 128 34 48 621.6 0 48 35 49 621.6 0 49 36 76 621.6 0 76 37 43 621.6 0 43 38 83 621.6 0 83 39 79 621.6 0 79 40 68 621.6 0 68 41 81 621.6 0 81 42 55 621.6 0 55 43 94 621.6 0 94 44 190 621.6 0 190 45 166 621.6 0 166 46 261 621.6 0 261 47 48 621.6 0 48 48 51 621.6 0 51 49 48 621.6 0 48 50 68 621.6 0 68 51 84 621.6 0 84 52 61 621.6 0 61 53 84 621.6 0 84 54 59 621.6 0 59 55 41 621.6 0 41 56 65 621.6 0 65 57 98 621.6 0 98 58 602 621.6 0 602 59 343 621.6 0 343 60 313 621.6 0 313 61 59 621.6 0 59 62 52 621.6 0 52 63 41 621.6 0 41 64 57 621.6 0 57 65 49 621.6 0 49 66 65 621.6 0 65 67 67 621.6 0 67 68 62 621.6 0 62 69 56 621.6 0 56 70 64 621.6 0 64 71 72 621.6 0 72 72 72 621.6 0 72 73 121 621.6 0 121 74 58 621.6 0 58 75 87 621.6 0 87 76 50 621.6 0 50 77 82 621.6 0 82 78 52 621.6 0 52 79 54 621.6 0 54 80 58 621.6 0 58 81 63 621.6 0 63 82 63 621.6 0 63 83 86 621.6 0 86 84 71 621.6 0 71 85 75 621.6 0 75 86 69 621.6 0 69 87 61 621.6 0 61 88 54 621.6 0 54 89 64 621.6 0 64 90 74 621.6 0 74 91 107 621.6 0 107 92 101 621.6 0 101 93 117 621.6 0 117 94 97 621.6 0 97 95 97 621.6 0 97 96 67 621.6 0 67 97 100 621.6 0 100 98 101 621.6 0 101 99 88 621.6 0 88 100 54 621.6 0 54 101 67 621.6 0 67 102 67 621.6 0 67 103 61 621.6 0 61 104 69 621.6 0 69 105 106 621.6 0 106 106 456 621.6 0 456 107 269 621.6 0 269 108 419 621.6 0 419 109 104 621.6 0 104 110 91 621.6 0 91 111 69 621.6 0 69 112 74 621.6 0 74 113 80 621.6 0 80 114 203 621.6 0 203 115 145 621.6 0 145 116 141 621.6 0 141 117 71 621.6 0 71 118 78 621.6 0 78 119 85 621.6 0 85 120 54 621.6 0 54 121 83 621.6 0 83 122 60 621.6 0 60 123 101 621.6 0 101 124 68 621.6 0 68 125 112 621.6 0 112 126 109 621.6 0 109 127 161 621.6 0 161 128 1787 621.6 0 1787 129 884 621.6 0 884 130 990 621.6 0 990 131 98 621.6 0 98 132 92 621.6 0 92 133 104 621.6 0 104 134 192 621.6 0 192 135 181 621.6 0 181 136 321 621.6 0 321 137 216 621.6 0 216 138 266 621.6 0 266 139 76 621.6 0 76 140 73 621.6 0 73 141 266 621.6 0 266 142 126 621.6 0 126 143 219 621.6 0 219 144 153 621.6 0 153 145 89 621.6 0 89 146 88 621.6 0 88 147 162 621.6 0 162 148 1783 621.6 0 1783 149 1175 621.6 0 1175 150 1300 621.6 0 1300 151 67 621.6 0 67 152 52 621.6 0 52 153 107 621.6 0 107 154 79 621.6 0 79 155 370 621.6 0 370 156 192 621.6 0 192 157 280 621.6 0 280 158 218 621.6 0 218 159 58 621.6 0 58 160 44 621.6 0 44 161 121 621.6 0 121 162 144 621.6 0 144 163 559 621.6 0 559 164 314 621.6 0 314 165 366 621.6 0 366 166 64 621.6 0 64 167 50 621.6 0 50 168 87 621.6 0 87 169 64 621.6 0 64 170 93 621.6 0 93 171 31 621.6 0 31 172 42 621.6 0 42 173 50 621.6 0 50 174 57 621.6 0 57 175 50 621.6 0 50 176 43 621.6 0 43 177 67 621.6 0 67 178 67 621.6 0 67 179 102 621.6 0 102 180 324 621.6 0 324 181 236 621.6 0 236 182 267 621.6 0 267 183 194 621.6 0 194 184 58 621.6 0 58 185 95 621.6 0 95 186 61 621.6 0 61 187 48 621.6 0 48 188 44 621.6 0 44 189 69 621.6 0 69 190 63 621.6 0 63 191 75 621.6 0 75 192 79 621.6 0 79 193 93 621.6 0 93 194 63 621.6 0 63 195 49 621.6 0 49 196 63 621.6 0 63 197 61 621.6 0 61 198 58 621.6 0 58 199 54 621.6 0 54 200 55 621.6 0 55 201 59 621.6 0 59 202 132 621.6 0 132 203 89 621.6 0 89 204 154 621.6 0 154 205 45 621.6 0 45 206 38 621.6 0 38 207 43 621.6 0 43 208 75 621.6 0 75 209 89 621.6 0 89 210 71 621.6 0 71 211 57 621.6 0 57 212 74 621.6 0 74 213 109 621.6 0 109 214 212 621.6 0 212 215 269 621.6 0 269 216 256 621.6 0 256 217 100 621.6 0 100 218 56 621.6 0 56 219 44 621.6 0 44 220 53 621.6 0 53 221 71 621.6 0 71 222 92 621.6 0 92 223 90 621.6 0 90 224 112 621.6 0 112 225 87 621.6 0 87 226 80 621.6 0 80 227 77 621.6 0 77 228 74 621.6 0 74 229 70 621.6 0 70 230 61 621.6 0 61 231 69 621.6 0 69 232 96 621.6 0 96 233 151 621.6 0 151 234 194 621.6 0 194 235 149 621.6 0 149 236 85 621.6 0 85 237 73 621.6 0 73 238 77 621.6 0 77 239 82 621.6 0 82 240 56 621.6 0 56 241 73 621.6 0 73 242 96 621.6 0 96 243 91 621.6 0 91 244 112 621.6 0 112 245 73 621.6 0 73 246 69 621.6 0 69 247 127 621.6 0 127 248 122 621.6 0 122 249 81 621.6 0 81 250 56 621.6 0 56 251 79 621.6 0 79 252 90 621.6 0 90 253 170 621.6 0 170 254 116 621.6 0 116 255 144 621.6 0 144 256 99 621.6 0 99 257 113 621.6 0 113 258 122 621.6 0 122 259 94 621.6 0 94 260 124 621.6 0 124 261 1095 621.6 0 1095 262 519 621.6 0 519 263 749 621.6 0 749 264 112 621.6 0 112 265 82 621.6 0 82 266 94 621.6 0 94 267 93 621.6 0 93 268 74 621.6 0 74 269 122 621.6 0 122 270 163 621.6 0 163 271 836 621.6 0 836 272 376 621.6 0 376 273 553 621.6 0 553 274 107 621.6 0 107 275 70 621.6 0 70 276 70 621.6 0 70 277 77 621.6 0 77 278 118 621.6 0 118 279 184 621.6 0 184 280 165 621.6 0 165 281 131 621.6 0 131 282 104 621.6 0 104 283 129 621.6 0 129 284 136 621.6 0 136 285 128 621.6 0 128 286 80 621.6 0 80 287 95 621.6 0 95 288 116 621.6 0 116 289 77 621.6 0 77 290 165 621.6 0 165 291 121 621.6 0 121 292 266 621.6 0 266 293 186 621.6 0 186 294 389 621.6 0 389 295 1132 621.6 0 1132 296 1039 621.6 0 1039 297 1190 621.6 0 1190 298 340 621.6 0 340 299 65 621.6 0 65 300 88 621.6 0 88 301 64 621.6 0 64 Finished in 1.56 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 35,572 Reads with adapters: 3,366 (9.5%) Reads that were too short: 23 (0.1%) Reads written (passing filters): 3,343 (9.4%) Total basepairs processed: 7,315,271 bp Total written (filtered): 908,296 bp (12.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3366 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 0 2 19 46 0.0 3 0 40 2 4 20 3268 0.0 4 3040 180 32 9 7 21 5 0.0 4 1 3 1 22 2 0.0 4 0 0 1 0 1 23 10 0.0 4 1 0 0 6 3 25 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 73 2 0.0 4 0 0 0 0 2 83 1 0.0 4 0 0 1 93 2 0.0 4 0 0 0 0 2 98 1 0.0 4 0 0 1 105 1 0.0 4 0 0 0 1 109 1 0.0 4 0 0 0 0 1 113 3 0.0 4 0 0 0 1 2 119 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 1 130 7 0.0 4 0 0 0 0 7 132 1 0.0 4 0 0 0 0 1 141 2 0.0 4 0 0 0 0 2 154 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.13 s (39 us/read; 1.54 M reads/minute). === Summary === Total reads processed: 3,343 Reads with adapters: 931 (27.8%) Reads written (passing filters): 3,343 (100.0%) Total basepairs processed: 908,296 bp Total written (filtered): 898,496 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 931 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.9% C: 10.5% G: 84.9% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 13.1 0 1 6 1 0.8 0 1 9 363 0.0 0 36 327 10 508 0.0 1 35 473 11 52 0.0 1 1 51 12 2 0.0 1 0 2 208 3 0.0 1 3 223 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.