This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGACGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.08 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 86,534 (3.4%) Reads that were too short: 35,050 (1.4%) Reads written (passing filters): 51,484 (2.0%) Total basepairs processed: 766,381,518 bp Total written (filtered): 12,251,960 bp (1.6%) === Adapter 1 === Sequence: CGACGT; Type: regular 5'; Length: 6; Trimmed: 86534 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1025 621.6 0 1025 7 12878 621.6 0 12878 8 977 621.6 0 977 9 14464 621.6 0 14464 10 56 621.6 0 56 11 29 621.6 0 29 12 37 621.6 0 37 13 35 621.6 0 35 14 45 621.6 0 45 15 33 621.6 0 33 16 125 621.6 0 125 17 56 621.6 0 56 18 45 621.6 0 45 19 41 621.6 0 41 20 19 621.6 0 19 21 40 621.6 0 40 22 38 621.6 0 38 23 33 621.6 0 33 24 27 621.6 0 27 25 64 621.6 0 64 26 45 621.6 0 45 27 37 621.6 0 37 28 30 621.6 0 30 29 39 621.6 0 39 30 39 621.6 0 39 31 40 621.6 0 40 32 46 621.6 0 46 33 37 621.6 0 37 34 42 621.6 0 42 35 38 621.6 0 38 36 40 621.6 0 40 37 33 621.6 0 33 38 34 621.6 0 34 39 26 621.6 0 26 40 29 621.6 0 29 41 41 621.6 0 41 42 41 621.6 0 41 43 38 621.6 0 38 44 44 621.6 0 44 45 30 621.6 0 30 46 33 621.6 0 33 47 46 621.6 0 46 48 53 621.6 0 53 49 43 621.6 0 43 50 63 621.6 0 63 51 49 621.6 0 49 52 34 621.6 0 34 53 46 621.6 0 46 54 44 621.6 0 44 55 36 621.6 0 36 56 34 621.6 0 34 57 46 621.6 0 46 58 30 621.6 0 30 59 49 621.6 0 49 60 40 621.6 0 40 61 109 621.6 0 109 62 52 621.6 0 52 63 54 621.6 0 54 64 37 621.6 0 37 65 53 621.6 0 53 66 92 621.6 0 92 67 1238 621.6 0 1238 68 229 621.6 0 229 69 245 621.6 0 245 70 35 621.6 0 35 71 28 621.6 0 28 72 47 621.6 0 47 73 39 621.6 0 39 74 43 621.6 0 43 75 40 621.6 0 40 76 48 621.6 0 48 77 34 621.6 0 34 78 34 621.6 0 34 79 33 621.6 0 33 80 20 621.6 0 20 81 60 621.6 0 60 82 33 621.6 0 33 83 34 621.6 0 34 84 38 621.6 0 38 85 87 621.6 0 87 86 154 621.6 0 154 87 51 621.6 0 51 88 32 621.6 0 32 89 54 621.6 0 54 90 33 621.6 0 33 91 24 621.6 0 24 92 111 621.6 0 111 93 58 621.6 0 58 94 81 621.6 0 81 95 40 621.6 0 40 96 50 621.6 0 50 97 43 621.6 0 43 98 55 621.6 0 55 99 29 621.6 0 29 100 52 621.6 0 52 101 16 621.6 0 16 102 41 621.6 0 41 103 34 621.6 0 34 104 29 621.6 0 29 105 44 621.6 0 44 106 45 621.6 0 45 107 33 621.6 0 33 108 28 621.6 0 28 109 37 621.6 0 37 110 28 621.6 0 28 111 38 621.6 0 38 112 34 621.6 0 34 113 44 621.6 0 44 114 28 621.6 0 28 115 35 621.6 0 35 116 38 621.6 0 38 117 32 621.6 0 32 118 64 621.6 0 64 119 76 621.6 0 76 120 105 621.6 0 105 121 52 621.6 0 52 122 56 621.6 0 56 123 67 621.6 0 67 124 35 621.6 0 35 125 36 621.6 0 36 126 61 621.6 0 61 127 64 621.6 0 64 128 85 621.6 0 85 129 74 621.6 0 74 130 56 621.6 0 56 131 89 621.6 0 89 132 151 621.6 0 151 133 162 621.6 0 162 134 322 621.6 0 322 135 44 621.6 0 44 136 34 621.6 0 34 137 49 621.6 0 49 138 65 621.6 0 65 139 74 621.6 0 74 140 41 621.6 0 41 141 48 621.6 0 48 142 43 621.6 0 43 143 39 621.6 0 39 144 57 621.6 0 57 145 83 621.6 0 83 146 109 621.6 0 109 147 86 621.6 0 86 148 77 621.6 0 77 149 75 621.6 0 75 150 103 621.6 0 103 151 127 621.6 0 127 152 1851 621.6 0 1851 153 1339 621.6 0 1339 154 1398 621.6 0 1398 155 205 621.6 0 205 156 43 621.6 0 43 157 39 621.6 0 39 158 151 621.6 0 151 159 61 621.6 0 61 160 81 621.6 0 81 161 78 621.6 0 78 162 69 621.6 0 69 163 402 621.6 0 402 164 328 621.6 0 328 165 510 621.6 0 510 166 204 621.6 0 204 167 133 621.6 0 133 168 80 621.6 0 80 169 54 621.6 0 54 170 65 621.6 0 65 171 62 621.6 0 62 172 70 621.6 0 70 173 99 621.6 0 99 174 101 621.6 0 101 175 84 621.6 0 84 176 54 621.6 0 54 177 85 621.6 0 85 178 110 621.6 0 110 179 95 621.6 0 95 180 159 621.6 0 159 181 139 621.6 0 139 182 103 621.6 0 103 183 528 621.6 0 528 184 590 621.6 0 590 185 702 621.6 0 702 186 427 621.6 0 427 187 121 621.6 0 121 188 56 621.6 0 56 189 68 621.6 0 68 190 75 621.6 0 75 191 97 621.6 0 97 192 290 621.6 0 290 193 291 621.6 0 291 194 438 621.6 0 438 195 198 621.6 0 198 196 72 621.6 0 72 197 66 621.6 0 66 198 210 621.6 0 210 199 268 621.6 0 268 200 225 621.6 0 225 201 126 621.6 0 126 202 131 621.6 0 131 203 102 621.6 0 102 204 114 621.6 0 114 205 185 621.6 0 185 206 220 621.6 0 220 207 128 621.6 0 128 208 113 621.6 0 113 209 95 621.6 0 95 210 93 621.6 0 93 211 45 621.6 0 45 212 99 621.6 0 99 213 81 621.6 0 81 214 123 621.6 0 123 215 196 621.6 0 196 216 171 621.6 0 171 217 418 621.6 0 418 218 755 621.6 0 755 219 9455 621.6 0 9455 220 4352 621.6 0 4352 221 5012 621.6 0 5012 222 173 621.6 0 173 223 91 621.6 0 91 224 140 621.6 0 140 225 61 621.6 0 61 226 62 621.6 0 62 227 67 621.6 0 67 228 64 621.6 0 64 229 85 621.6 0 85 230 94 621.6 0 94 231 94 621.6 0 94 232 88 621.6 0 88 233 69 621.6 0 69 234 123 621.6 0 123 235 117 621.6 0 117 236 111 621.6 0 111 237 133 621.6 0 133 238 131 621.6 0 131 239 170 621.6 0 170 240 154 621.6 0 154 241 96 621.6 0 96 242 81 621.6 0 81 243 60 621.6 0 60 244 80 621.6 0 80 245 72 621.6 0 72 246 86 621.6 0 86 247 111 621.6 0 111 248 253 621.6 0 253 249 391 621.6 0 391 250 505 621.6 0 505 251 252 621.6 0 252 252 212 621.6 0 212 253 249 621.6 0 249 254 305 621.6 0 305 255 137 621.6 0 137 256 123 621.6 0 123 257 96 621.6 0 96 258 68 621.6 0 68 259 98 621.6 0 98 260 67 621.6 0 67 261 78 621.6 0 78 262 81 621.6 0 81 263 75 621.6 0 75 264 74 621.6 0 74 265 54 621.6 0 54 266 110 621.6 0 110 267 143 621.6 0 143 268 121 621.6 0 121 269 104 621.6 0 104 270 99 621.6 0 99 271 104 621.6 0 104 272 208 621.6 0 208 273 256 621.6 0 256 274 239 621.6 0 239 275 124 621.6 0 124 276 75 621.6 0 75 277 103 621.6 0 103 278 230 621.6 0 230 279 638 621.6 0 638 280 725 621.6 0 725 281 729 621.6 0 729 282 206 621.6 0 206 283 151 621.6 0 151 284 152 621.6 0 152 285 116 621.6 0 116 286 116 621.6 0 116 287 201 621.6 0 201 288 122 621.6 0 122 289 154 621.6 0 154 290 116 621.6 0 116 291 106 621.6 0 106 292 218 621.6 0 218 293 173 621.6 0 173 294 253 621.6 0 253 295 152 621.6 0 152 296 125 621.6 0 125 297 136 621.6 0 136 298 125 621.6 0 125 299 204 621.6 0 204 300 334 621.6 0 334 301 263 621.6 0 263 Finished in 2.52 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 51,484 Reads with adapters: 13,650 (26.5%) Reads that were too short: 28 (0.1%) Reads written (passing filters): 13,622 (26.5%) Total basepairs processed: 12,251,960 bp Total written (filtered): 3,732,903 bp (30.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 13650 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 12 0.0 3 0 0 6 3 3 19 219 0.0 3 0 184 22 9 4 20 13324 0.0 4 12425 724 135 29 11 21 31 0.0 4 3 24 3 0 1 22 4 0.0 4 2 0 1 1 23 24 0.0 4 0 0 0 14 10 24 1 0.0 4 0 0 0 1 25 1 0.0 4 1 27 1 0.0 4 0 0 1 28 1 0.0 4 0 0 0 0 1 29 1 0.0 4 1 42 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 83 1 0.0 4 1 89 4 0.0 4 0 0 0 0 4 90 3 0.0 4 0 0 0 0 3 103 3 0.0 4 0 0 0 0 3 104 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 137 2 0.0 4 0 0 0 0 2 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.47 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 13,622 Reads with adapters: 1,023 (7.5%) Reads written (passing filters): 13,622 (100.0%) Total basepairs processed: 3,732,903 bp Total written (filtered): 3,720,597 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1023 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.1% C: 13.2% G: 76.7% T: 2.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 74 212.8 0 74 4 19 53.2 0 19 5 2 13.3 0 2 9 3 0.1 0 0 3 10 21 0.0 1 1 20 11 590 0.0 1 28 562 12 296 0.0 1 3 293 13 8 0.0 1 0 8 14 1 0.0 1 0 1 152 4 0.0 1 3 1 153 1 0.0 1 1 210 4 0.0 1 4