This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.77 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 269,418 (10.6%) Reads that were too short: 32,988 (1.3%) Reads written (passing filters): 236,430 (9.3%) Total basepairs processed: 766,381,518 bp Total written (filtered): 51,678,217 bp (6.7%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 269418 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 199 621.6 0 199 7 513 621.6 0 513 8 4779 621.6 0 4779 9 9192 621.6 0 9192 10 35667 621.6 0 35667 11 45 621.6 0 45 12 42 621.6 0 42 13 22 621.6 0 22 14 17 621.6 0 17 15 17 621.6 0 17 16 26 621.6 0 26 17 27 621.6 0 27 18 19 621.6 0 19 19 47 621.6 0 47 20 31 621.6 0 31 21 50 621.6 0 50 22 42 621.6 0 42 23 36 621.6 0 36 24 55 621.6 0 55 25 46 621.6 0 46 26 58 621.6 0 58 27 56 621.6 0 56 28 41 621.6 0 41 29 34 621.6 0 34 30 43 621.6 0 43 31 39 621.6 0 39 32 37 621.6 0 37 33 41 621.6 0 41 34 23 621.6 0 23 35 41 621.6 0 41 36 40 621.6 0 40 37 24 621.6 0 24 38 51 621.6 0 51 39 51 621.6 0 51 40 86 621.6 0 86 41 108 621.6 0 108 42 49 621.6 0 49 43 44 621.6 0 44 44 19 621.6 0 19 45 28 621.6 0 28 46 27 621.6 0 27 47 39 621.6 0 39 48 85 621.6 0 85 49 58 621.6 0 58 50 61 621.6 0 61 51 53 621.6 0 53 52 116 621.6 0 116 53 85 621.6 0 85 54 103 621.6 0 103 55 30 621.6 0 30 56 37 621.6 0 37 57 49 621.6 0 49 58 47 621.6 0 47 59 42 621.6 0 42 60 70 621.6 0 70 61 49 621.6 0 49 62 44 621.6 0 44 63 41 621.6 0 41 64 35 621.6 0 35 65 54 621.6 0 54 66 90 621.6 0 90 67 55 621.6 0 55 68 138 621.6 0 138 69 99 621.6 0 99 70 157 621.6 0 157 71 363 621.6 0 363 72 596 621.6 0 596 73 278 621.6 0 278 74 101 621.6 0 101 75 109 621.6 0 109 76 157 621.6 0 157 77 212 621.6 0 212 78 414 621.6 0 414 79 872 621.6 0 872 80 232 621.6 0 232 81 309 621.6 0 309 82 547 621.6 0 547 83 262 621.6 0 262 84 292 621.6 0 292 85 652 621.6 0 652 86 561 621.6 0 561 87 670 621.6 0 670 88 599 621.6 0 599 89 905 621.6 0 905 90 1524 621.6 0 1524 91 2010 621.6 0 2010 92 46998 621.6 0 46998 93 37860 621.6 0 37860 94 65978 621.6 0 65978 95 804 621.6 0 804 96 34 621.6 0 34 97 25 621.6 0 25 98 39 621.6 0 39 99 21 621.6 0 21 100 27 621.6 0 27 101 23 621.6 0 23 102 22 621.6 0 22 103 28 621.6 0 28 104 26 621.6 0 26 105 24 621.6 0 24 106 15 621.6 0 15 107 23 621.6 0 23 108 67 621.6 0 67 109 73 621.6 0 73 110 24 621.6 0 24 111 20 621.6 0 20 112 46 621.6 0 46 113 23 621.6 0 23 114 37 621.6 0 37 115 26 621.6 0 26 116 22 621.6 0 22 117 30 621.6 0 30 118 23 621.6 0 23 119 11 621.6 0 11 120 20 621.6 0 20 121 11 621.6 0 11 122 21 621.6 0 21 123 21 621.6 0 21 124 20 621.6 0 20 125 35 621.6 0 35 126 33 621.6 0 33 127 19 621.6 0 19 128 45 621.6 0 45 129 30 621.6 0 30 130 20 621.6 0 20 131 20 621.6 0 20 132 26 621.6 0 26 133 23 621.6 0 23 134 16 621.6 0 16 135 41 621.6 0 41 136 33 621.6 0 33 137 43 621.6 0 43 138 59 621.6 0 59 139 43 621.6 0 43 140 35 621.6 0 35 141 27 621.6 0 27 142 27 621.6 0 27 143 41 621.6 0 41 144 34 621.6 0 34 145 31 621.6 0 31 146 21 621.6 0 21 147 30 621.6 0 30 148 42 621.6 0 42 149 39 621.6 0 39 150 31 621.6 0 31 151 43 621.6 0 43 152 22 621.6 0 22 153 22 621.6 0 22 154 27 621.6 0 27 155 24 621.6 0 24 156 33 621.6 0 33 157 34 621.6 0 34 158 22 621.6 0 22 159 45 621.6 0 45 160 26 621.6 0 26 161 28 621.6 0 28 162 22 621.6 0 22 163 28 621.6 0 28 164 20 621.6 0 20 165 42 621.6 0 42 166 21 621.6 0 21 167 29 621.6 0 29 168 23 621.6 0 23 169 26 621.6 0 26 170 17 621.6 0 17 171 39 621.6 0 39 172 24 621.6 0 24 173 33 621.6 0 33 174 26 621.6 0 26 175 43 621.6 0 43 176 47 621.6 0 47 177 141 621.6 0 141 178 298 621.6 0 298 179 153 621.6 0 153 180 142 621.6 0 142 181 37 621.6 0 37 182 39 621.6 0 39 183 72 621.6 0 72 184 58 621.6 0 58 185 62 621.6 0 62 186 87 621.6 0 87 187 147 621.6 0 147 188 293 621.6 0 293 189 99 621.6 0 99 190 153 621.6 0 153 191 296 621.6 0 296 192 225 621.6 0 225 193 89 621.6 0 89 194 167 621.6 0 167 195 167 621.6 0 167 196 190 621.6 0 190 197 138 621.6 0 138 198 237 621.6 0 237 199 367 621.6 0 367 200 632 621.6 0 632 201 12975 621.6 0 12975 202 6317 621.6 0 6317 203 13913 621.6 0 13913 204 225 621.6 0 225 205 494 621.6 0 494 206 640 621.6 0 640 207 664 621.6 0 664 208 227 621.6 0 227 209 110 621.6 0 110 210 89 621.6 0 89 211 164 621.6 0 164 212 62 621.6 0 62 213 67 621.6 0 67 214 31 621.6 0 31 215 66 621.6 0 66 216 57 621.6 0 57 217 155 621.6 0 155 218 67 621.6 0 67 219 25 621.6 0 25 220 71 621.6 0 71 221 168 621.6 0 168 222 56 621.6 0 56 223 222 621.6 0 222 224 63 621.6 0 63 225 284 621.6 0 284 226 890 621.6 0 890 227 798 621.6 0 798 228 490 621.6 0 490 229 220 621.6 0 220 230 21 621.6 0 21 231 19 621.6 0 19 232 24 621.6 0 24 233 25 621.6 0 25 234 21 621.6 0 21 235 34 621.6 0 34 236 45 621.6 0 45 237 29 621.6 0 29 238 79 621.6 0 79 239 35 621.6 0 35 240 21 621.6 0 21 241 25 621.6 0 25 242 25 621.6 0 25 243 39 621.6 0 39 244 19 621.6 0 19 245 27 621.6 0 27 246 48 621.6 0 48 247 42 621.6 0 42 248 32 621.6 0 32 249 60 621.6 0 60 250 34 621.6 0 34 251 28 621.6 0 28 252 20 621.6 0 20 253 39 621.6 0 39 254 31 621.6 0 31 255 25 621.6 0 25 256 30 621.6 0 30 257 37 621.6 0 37 258 68 621.6 0 68 259 407 621.6 0 407 260 618 621.6 0 618 261 782 621.6 0 782 262 375 621.6 0 375 263 114 621.6 0 114 264 27 621.6 0 27 265 35 621.6 0 35 266 32 621.6 0 32 267 26 621.6 0 26 268 26 621.6 0 26 269 38 621.6 0 38 270 73 621.6 0 73 271 123 621.6 0 123 272 120 621.6 0 120 273 52 621.6 0 52 274 36 621.6 0 36 275 76 621.6 0 76 276 30 621.6 0 30 277 54 621.6 0 54 278 42 621.6 0 42 279 41 621.6 0 41 280 107 621.6 0 107 281 99 621.6 0 99 282 88 621.6 0 88 283 190 621.6 0 190 284 107 621.6 0 107 285 139 621.6 0 139 286 151 621.6 0 151 287 304 621.6 0 304 288 30 621.6 0 30 289 32 621.6 0 32 290 27 621.6 0 27 291 24 621.6 0 24 292 21 621.6 0 21 293 47 621.6 0 47 294 36 621.6 0 36 295 39 621.6 0 39 296 81 621.6 0 81 297 361 621.6 0 361 298 149 621.6 0 149 299 166 621.6 0 166 300 24 621.6 0 24 301 22 621.6 0 22 Finished in 8.64 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 236,430 Reads with adapters: 4,694 (2.0%) Reads that were too short: 89 (0.0%) Reads written (passing filters): 4,605 (1.9%) Total basepairs processed: 51,678,217 bp Total written (filtered): 1,088,492 bp (2.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4694 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 28 0.0 3 0 26 2 17 3458 0.0 3 3399 57 2 18 8 0.0 3 0 7 1 20 6 0.0 3 3 0 0 3 25 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 68 1 0.0 3 1 72 1 0.0 3 0 0 0 1 74 12 0.0 3 0 0 0 12 75 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 77 6 0.0 3 1 0 0 5 79 29 0.0 3 0 0 0 29 81 1 0.0 3 0 0 0 1 82 5 0.0 3 0 0 0 5 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 98 8 0.0 3 0 0 0 8 99 677 0.0 3 0 0 2 675 100 255 0.0 3 0 0 0 255 101 3 0.0 3 1 0 0 2 102 46 0.0 3 0 0 2 44 103 37 0.0 3 0 0 0 37 104 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 1 125 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 135 3 0.0 3 0 0 1 2 137 2 0.0 3 0 0 0 2 141 1 0.0 3 1 144 5 0.0 3 5 145 2 0.0 3 2 146 1 0.0 3 0 0 1 149 1 0.0 3 0 0 0 1 150 6 0.0 3 0 0 0 6 151 5 0.0 3 0 0 0 5 153 3 0.0 3 0 0 0 3 158 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 160 14 0.0 3 0 0 0 14 161 2 0.0 3 0 0 0 2 175 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 200 24 0.0 3 0 0 0 24 201 3 0.0 3 0 0 0 3 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 4,605 Reads with adapters: 17 (0.4%) Reads written (passing filters): 4,605 (100.0%) Total basepairs processed: 1,088,492 bp Total written (filtered): 1,088,435 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 17 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.5% C: 0.0% G: 23.5% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 11 72.0 0 11 4 6 18.0 0 6