This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAGAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.96 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 327,082 (12.8%) Reads that were too short: 14,371 (0.6%) Reads written (passing filters): 312,711 (12.3%) Total basepairs processed: 766,381,518 bp Total written (filtered): 77,222,561 bp (10.1%) === Adapter 1 === Sequence: AGAGAC; Type: regular 5'; Length: 6; Trimmed: 327082 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1774 621.6 0 1774 7 69015 621.6 0 69015 8 50304 621.6 0 50304 9 94 621.6 0 94 10 133 621.6 0 133 11 23 621.6 0 23 12 19 621.6 0 19 13 46 621.6 0 46 14 7 621.6 0 7 15 11 621.6 0 11 16 5 621.6 0 5 17 9 621.6 0 9 18 6 621.6 0 6 19 14 621.6 0 14 20 13 621.6 0 13 21 37 621.6 0 37 22 17 621.6 0 17 23 10 621.6 0 10 24 13 621.6 0 13 25 15 621.6 0 15 26 7 621.6 0 7 27 19 621.6 0 19 28 7 621.6 0 7 29 13 621.6 0 13 30 23 621.6 0 23 31 6 621.6 0 6 32 7 621.6 0 7 33 12 621.6 0 12 34 15 621.6 0 15 35 25 621.6 0 25 36 16 621.6 0 16 37 9 621.6 0 9 38 8 621.6 0 8 39 20 621.6 0 20 40 22 621.6 0 22 41 29 621.6 0 29 42 84 621.6 0 84 43 48 621.6 0 48 44 46 621.6 0 46 45 64 621.6 0 64 46 48 621.6 0 48 47 40 621.6 0 40 48 20 621.6 0 20 49 87 621.6 0 87 50 39 621.6 0 39 51 63 621.6 0 63 52 456 621.6 0 456 53 76 621.6 0 76 54 39 621.6 0 39 55 102 621.6 0 102 56 24 621.6 0 24 57 18 621.6 0 18 58 19 621.6 0 19 59 72 621.6 0 72 60 61 621.6 0 61 61 263 621.6 0 263 62 681 621.6 0 681 63 135 621.6 0 135 64 258 621.6 0 258 65 586 621.6 0 586 66 598 621.6 0 598 67 2261 621.6 0 2261 68 261 621.6 0 261 69 211 621.6 0 211 70 69 621.6 0 69 71 140 621.6 0 140 72 348 621.6 0 348 73 377 621.6 0 377 74 694 621.6 0 694 75 1014 621.6 0 1014 76 1027 621.6 0 1027 77 2683 621.6 0 2683 78 6842 621.6 0 6842 79 68790 621.6 0 68790 80 30118 621.6 0 30118 81 57240 621.6 0 57240 82 419 621.6 0 419 83 236 621.6 0 236 84 43 621.6 0 43 85 26 621.6 0 26 86 45 621.6 0 45 87 46 621.6 0 46 88 26 621.6 0 26 89 37 621.6 0 37 90 52 621.6 0 52 91 11 621.6 0 11 92 22 621.6 0 22 93 21 621.6 0 21 94 16 621.6 0 16 95 28 621.6 0 28 96 27 621.6 0 27 97 11 621.6 0 11 98 13 621.6 0 13 99 20 621.6 0 20 100 14 621.6 0 14 101 27 621.6 0 27 102 17 621.6 0 17 103 33 621.6 0 33 104 23 621.6 0 23 105 13 621.6 0 13 106 23 621.6 0 23 107 10 621.6 0 10 108 22 621.6 0 22 109 26 621.6 0 26 110 40 621.6 0 40 111 30 621.6 0 30 112 32 621.6 0 32 113 41 621.6 0 41 114 46 621.6 0 46 115 50 621.6 0 50 116 292 621.6 0 292 117 170 621.6 0 170 118 528 621.6 0 528 119 70 621.6 0 70 120 81 621.6 0 81 121 222 621.6 0 222 122 147 621.6 0 147 123 842 621.6 0 842 124 742 621.6 0 742 125 601 621.6 0 601 126 146 621.6 0 146 127 63 621.6 0 63 128 53 621.6 0 53 129 84 621.6 0 84 130 470 621.6 0 470 131 400 621.6 0 400 132 593 621.6 0 593 133 60 621.6 0 60 134 32 621.6 0 32 135 54 621.6 0 54 136 49 621.6 0 49 137 73 621.6 0 73 138 50 621.6 0 50 139 62 621.6 0 62 140 104 621.6 0 104 141 122 621.6 0 122 142 192 621.6 0 192 143 73 621.6 0 73 144 54 621.6 0 54 145 113 621.6 0 113 146 92 621.6 0 92 147 162 621.6 0 162 148 107 621.6 0 107 149 117 621.6 0 117 150 649 621.6 0 649 151 642 621.6 0 642 152 1952 621.6 0 1952 153 84 621.6 0 84 154 23 621.6 0 23 155 48 621.6 0 48 156 41 621.6 0 41 157 48 621.6 0 48 158 70 621.6 0 70 159 34 621.6 0 34 160 40 621.6 0 40 161 31 621.6 0 31 162 49 621.6 0 49 163 50 621.6 0 50 164 38 621.6 0 38 165 72 621.6 0 72 166 87 621.6 0 87 167 125 621.6 0 125 168 96 621.6 0 96 169 90 621.6 0 90 170 99 621.6 0 99 171 86 621.6 0 86 172 123 621.6 0 123 173 106 621.6 0 106 174 182 621.6 0 182 175 33 621.6 0 33 176 34 621.6 0 34 177 36 621.6 0 36 178 44 621.6 0 44 179 37 621.6 0 37 180 45 621.6 0 45 181 38 621.6 0 38 182 66 621.6 0 66 183 51 621.6 0 51 184 50 621.6 0 50 185 44 621.6 0 44 186 123 621.6 0 123 187 80 621.6 0 80 188 72 621.6 0 72 189 41 621.6 0 41 190 41 621.6 0 41 191 41 621.6 0 41 192 58 621.6 0 58 193 71 621.6 0 71 194 56 621.6 0 56 195 48 621.6 0 48 196 47 621.6 0 47 197 60 621.6 0 60 198 82 621.6 0 82 199 55 621.6 0 55 200 78 621.6 0 78 201 44 621.6 0 44 202 36 621.6 0 36 203 49 621.6 0 49 204 37 621.6 0 37 205 61 621.6 0 61 206 44 621.6 0 44 207 49 621.6 0 49 208 55 621.6 0 55 209 55 621.6 0 55 210 103 621.6 0 103 211 65 621.6 0 65 212 74 621.6 0 74 213 77 621.6 0 77 214 79 621.6 0 79 215 66 621.6 0 66 216 86 621.6 0 86 217 171 621.6 0 171 218 299 621.6 0 299 219 2088 621.6 0 2088 220 132 621.6 0 132 221 108 621.6 0 108 222 112 621.6 0 112 223 126 621.6 0 126 224 135 621.6 0 135 225 140 621.6 0 140 226 710 621.6 0 710 227 601 621.6 0 601 228 973 621.6 0 973 229 150 621.6 0 150 230 113 621.6 0 113 231 82 621.6 0 82 232 88 621.6 0 88 233 89 621.6 0 89 234 70 621.6 0 70 235 37 621.6 0 37 236 55 621.6 0 55 237 69 621.6 0 69 238 59 621.6 0 59 239 69 621.6 0 69 240 77 621.6 0 77 241 74 621.6 0 74 242 64 621.6 0 64 243 71 621.6 0 71 244 54 621.6 0 54 245 88 621.6 0 88 246 174 621.6 0 174 247 187 621.6 0 187 248 180 621.6 0 180 249 100 621.6 0 100 250 147 621.6 0 147 251 120 621.6 0 120 252 105 621.6 0 105 253 119 621.6 0 119 254 60 621.6 0 60 255 101 621.6 0 101 256 99 621.6 0 99 257 69 621.6 0 69 258 57 621.6 0 57 259 108 621.6 0 108 260 103 621.6 0 103 261 131 621.6 0 131 262 86 621.6 0 86 263 89 621.6 0 89 264 93 621.6 0 93 265 92 621.6 0 92 266 160 621.6 0 160 267 99 621.6 0 99 268 101 621.6 0 101 269 74 621.6 0 74 270 65 621.6 0 65 271 62 621.6 0 62 272 83 621.6 0 83 273 92 621.6 0 92 274 96 621.6 0 96 275 100 621.6 0 100 276 121 621.6 0 121 277 178 621.6 0 178 278 104 621.6 0 104 279 159 621.6 0 159 280 164 621.6 0 164 281 193 621.6 0 193 282 80 621.6 0 80 283 62 621.6 0 62 284 77 621.6 0 77 285 85 621.6 0 85 286 106 621.6 0 106 287 132 621.6 0 132 288 110 621.6 0 110 289 85 621.6 0 85 290 124 621.6 0 124 291 135 621.6 0 135 292 188 621.6 0 188 293 127 621.6 0 127 294 114 621.6 0 114 295 88 621.6 0 88 296 85 621.6 0 85 297 125 621.6 0 125 298 164 621.6 0 164 299 202 621.6 0 202 300 242 621.6 0 242 301 129 621.6 0 129 Finished in 14.41 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 312,711 Reads with adapters: 62,676 (20.0%) Reads that were too short: 60 (0.0%) Reads written (passing filters): 62,616 (20.0%) Total basepairs processed: 77,222,561 bp Total written (filtered): 17,071,082 bp (22.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 62676 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 11 0.0 3 0 0 0 4 7 18 31 0.0 3 0 0 15 11 5 19 665 0.0 3 0 535 61 54 15 20 50641 0.0 4 47017 2873 539 171 41 21 261 0.0 4 27 197 18 14 5 22 10590 0.0 4 9877 547 118 36 12 23 243 0.0 4 3 22 0 158 60 24 7 0.0 4 0 0 4 1 2 25 31 0.0 4 0 0 0 18 13 26 1 0.0 4 0 0 0 1 27 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 33 1 0.0 4 1 36 1 0.0 4 1 37 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 84 1 0.0 4 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 95 2 0.0 4 0 0 0 0 2 110 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 1 117 2 0.0 4 0 0 0 0 2 120 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 111 0.0 4 0 0 0 0 111 129 4 0.0 4 0 0 0 0 4 140 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 1 200 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 3 0.0 4 0 0 0 0 3 237 1 0.0 4 0 0 0 0 1 242 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 0 2 259 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 282 9 0.0 4 0 0 0 0 9 283 3 0.0 4 0 0 0 0 3 284 1 0.0 4 0 0 0 0 1 285 2 0.0 4 0 0 0 0 2 288 2 0.0 4 0 0 0 0 2 289 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.27 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 62,616 Reads with adapters: 14,610 (23.3%) Reads written (passing filters): 62,616 (100.0%) Total basepairs processed: 17,071,082 bp Total written (filtered): 16,911,609 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 14610 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.9% C: 8.0% G: 88.2% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 60 978.4 0 60 4 35 244.6 0 35 5 3 61.1 0 3 6 5 15.3 0 5 7 2 3.8 0 2 8 7 1.0 0 7 9 852 0.2 0 54 798 10 7443 0.1 1 430 7013 11 6054 0.0 1 218 5836 12 61 0.0 1 1 60 13 36 0.0 1 0 36 14 2 0.0 1 0 2 15 1 0.0 1 0 1 16 1 0.0 1 0 1 22 1 0.0 1 1 40 1 0.0 1 1 150 4 0.0 1 3 1 151 12 0.0 1 4 8 178 2 0.0 1 0 2 190 2 0.0 1 0 2 207 1 0.0 1 1 208 2 0.0 1 2 209 4 0.0 1 2 2 210 1 0.0 1 1 224 4 0.0 1 4 227 9 0.0 1 9 228 2 0.0 1 2 270 1 0.0 1 0 1 271 1 0.0 1 0 1 272 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.