This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAGAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.49 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 391,591 (15.4%) Reads that were too short: 8,515 (0.3%) Reads written (passing filters): 383,076 (15.0%) Total basepairs processed: 766,381,518 bp Total written (filtered): 93,585,323 bp (12.2%) === Adapter 1 === Sequence: AGAGAC; Type: regular 5'; Length: 6; Trimmed: 391591 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 961 621.6 0 961 7 65858 621.6 0 65858 8 61014 621.6 0 61014 9 54 621.6 0 54 10 33 621.6 0 33 11 15 621.6 0 15 12 23 621.6 0 23 13 14 621.6 0 14 14 5 621.6 0 5 15 2 621.6 0 2 16 2 621.6 0 2 17 6 621.6 0 6 18 8 621.6 0 8 19 23 621.6 0 23 20 13 621.6 0 13 21 27 621.6 0 27 22 31 621.6 0 31 23 17 621.6 0 17 24 10 621.6 0 10 25 11 621.6 0 11 26 14 621.6 0 14 27 22 621.6 0 22 28 9 621.6 0 9 29 22 621.6 0 22 30 26 621.6 0 26 31 9 621.6 0 9 32 18 621.6 0 18 33 16 621.6 0 16 34 17 621.6 0 17 35 42 621.6 0 42 36 29 621.6 0 29 37 9 621.6 0 9 38 5 621.6 0 5 39 34 621.6 0 34 40 41 621.6 0 41 41 22 621.6 0 22 42 98 621.6 0 98 43 69 621.6 0 69 44 59 621.6 0 59 45 96 621.6 0 96 46 81 621.6 0 81 47 52 621.6 0 52 48 35 621.6 0 35 49 157 621.6 0 157 50 42 621.6 0 42 51 67 621.6 0 67 52 718 621.6 0 718 53 59 621.6 0 59 54 29 621.6 0 29 55 80 621.6 0 80 56 29 621.6 0 29 57 19 621.6 0 19 58 20 621.6 0 20 59 99 621.6 0 99 60 74 621.6 0 74 61 318 621.6 0 318 62 899 621.6 0 899 63 120 621.6 0 120 64 187 621.6 0 187 65 240 621.6 0 240 66 344 621.6 0 344 67 1136 621.6 0 1136 68 346 621.6 0 346 69 386 621.6 0 386 70 111 621.6 0 111 71 182 621.6 0 182 72 347 621.6 0 347 73 464 621.6 0 464 74 735 621.6 0 735 75 1276 621.6 0 1276 76 1337 621.6 0 1337 77 3475 621.6 0 3475 78 8879 621.6 0 8879 79 98665 621.6 0 98665 80 41626 621.6 0 41626 81 82032 621.6 0 82032 82 455 621.6 0 455 83 128 621.6 0 128 84 29 621.6 0 29 85 29 621.6 0 29 86 19 621.6 0 19 87 24 621.6 0 24 88 18 621.6 0 18 89 17 621.6 0 17 90 12 621.6 0 12 91 19 621.6 0 19 92 11 621.6 0 11 93 14 621.6 0 14 94 24 621.6 0 24 95 12 621.6 0 12 96 14 621.6 0 14 97 15 621.6 0 15 98 13 621.6 0 13 99 14 621.6 0 14 100 29 621.6 0 29 101 16 621.6 0 16 102 21 621.6 0 21 103 13 621.6 0 13 104 16 621.6 0 16 105 23 621.6 0 23 106 22 621.6 0 22 107 17 621.6 0 17 108 23 621.6 0 23 109 18 621.6 0 18 110 33 621.6 0 33 111 13 621.6 0 13 112 22 621.6 0 22 113 30 621.6 0 30 114 22 621.6 0 22 115 24 621.6 0 24 116 112 621.6 0 112 117 77 621.6 0 77 118 151 621.6 0 151 119 61 621.6 0 61 120 39 621.6 0 39 121 84 621.6 0 84 122 76 621.6 0 76 123 695 621.6 0 695 124 664 621.6 0 664 125 547 621.6 0 547 126 39 621.6 0 39 127 31 621.6 0 31 128 40 621.6 0 40 129 33 621.6 0 33 130 135 621.6 0 135 131 134 621.6 0 134 132 218 621.6 0 218 133 36 621.6 0 36 134 28 621.6 0 28 135 30 621.6 0 30 136 38 621.6 0 38 137 45 621.6 0 45 138 28 621.6 0 28 139 34 621.6 0 34 140 59 621.6 0 59 141 85 621.6 0 85 142 211 621.6 0 211 143 43 621.6 0 43 144 42 621.6 0 42 145 48 621.6 0 48 146 39 621.6 0 39 147 97 621.6 0 97 148 52 621.6 0 52 149 53 621.6 0 53 150 506 621.6 0 506 151 483 621.6 0 483 152 1582 621.6 0 1582 153 37 621.6 0 37 154 24 621.6 0 24 155 41 621.6 0 41 156 32 621.6 0 32 157 28 621.6 0 28 158 53 621.6 0 53 159 29 621.6 0 29 160 30 621.6 0 30 161 26 621.6 0 26 162 26 621.6 0 26 163 30 621.6 0 30 164 25 621.6 0 25 165 23 621.6 0 23 166 26 621.6 0 26 167 59 621.6 0 59 168 35 621.6 0 35 169 33 621.6 0 33 170 69 621.6 0 69 171 59 621.6 0 59 172 72 621.6 0 72 173 46 621.6 0 46 174 106 621.6 0 106 175 26 621.6 0 26 176 26 621.6 0 26 177 26 621.6 0 26 178 23 621.6 0 23 179 23 621.6 0 23 180 28 621.6 0 28 181 27 621.6 0 27 182 28 621.6 0 28 183 16 621.6 0 16 184 34 621.6 0 34 185 27 621.6 0 27 186 78 621.6 0 78 187 40 621.6 0 40 188 29 621.6 0 29 189 39 621.6 0 39 190 25 621.6 0 25 191 28 621.6 0 28 192 26 621.6 0 26 193 39 621.6 0 39 194 18 621.6 0 18 195 25 621.6 0 25 196 29 621.6 0 29 197 37 621.6 0 37 198 37 621.6 0 37 199 44 621.6 0 44 200 29 621.6 0 29 201 26 621.6 0 26 202 29 621.6 0 29 203 34 621.6 0 34 204 23 621.6 0 23 205 33 621.6 0 33 206 39 621.6 0 39 207 34 621.6 0 34 208 35 621.6 0 35 209 35 621.6 0 35 210 84 621.6 0 84 211 40 621.6 0 40 212 49 621.6 0 49 213 59 621.6 0 59 214 54 621.6 0 54 215 70 621.6 0 70 216 50 621.6 0 50 217 190 621.6 0 190 218 309 621.6 0 309 219 2998 621.6 0 2998 220 115 621.6 0 115 221 38 621.6 0 38 222 55 621.6 0 55 223 65 621.6 0 65 224 77 621.6 0 77 225 55 621.6 0 55 226 225 621.6 0 225 227 261 621.6 0 261 228 256 621.6 0 256 229 44 621.6 0 44 230 42 621.6 0 42 231 34 621.6 0 34 232 38 621.6 0 38 233 32 621.6 0 32 234 42 621.6 0 42 235 22 621.6 0 22 236 26 621.6 0 26 237 35 621.6 0 35 238 40 621.6 0 40 239 33 621.6 0 33 240 30 621.6 0 30 241 42 621.6 0 42 242 38 621.6 0 38 243 34 621.6 0 34 244 28 621.6 0 28 245 27 621.6 0 27 246 50 621.6 0 50 247 63 621.6 0 63 248 60 621.6 0 60 249 36 621.6 0 36 250 50 621.6 0 50 251 37 621.6 0 37 252 44 621.6 0 44 253 42 621.6 0 42 254 32 621.6 0 32 255 36 621.6 0 36 256 28 621.6 0 28 257 33 621.6 0 33 258 28 621.6 0 28 259 34 621.6 0 34 260 34 621.6 0 34 261 38 621.6 0 38 262 43 621.6 0 43 263 50 621.6 0 50 264 42 621.6 0 42 265 31 621.6 0 31 266 49 621.6 0 49 267 34 621.6 0 34 268 35 621.6 0 35 269 27 621.6 0 27 270 25 621.6 0 25 271 35 621.6 0 35 272 49 621.6 0 49 273 51 621.6 0 51 274 58 621.6 0 58 275 54 621.6 0 54 276 61 621.6 0 61 277 82 621.6 0 82 278 45 621.6 0 45 279 32 621.6 0 32 280 48 621.6 0 48 281 57 621.6 0 57 282 33 621.6 0 33 283 48 621.6 0 48 284 34 621.6 0 34 285 45 621.6 0 45 286 25 621.6 0 25 287 58 621.6 0 58 288 51 621.6 0 51 289 40 621.6 0 40 290 69 621.6 0 69 291 52 621.6 0 52 292 80 621.6 0 80 293 56 621.6 0 56 294 53 621.6 0 53 295 35 621.6 0 35 296 47 621.6 0 47 297 50 621.6 0 50 298 73 621.6 0 73 299 66 621.6 0 66 300 92 621.6 0 92 301 56 621.6 0 56 Finished in 15.46 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 383,076 Reads with adapters: 53,324 (13.9%) Reads that were too short: 67 (0.0%) Reads written (passing filters): 53,257 (13.9%) Total basepairs processed: 93,585,323 bp Total written (filtered): 14,740,684 bp (15.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 53324 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 16 0.0 3 0 0 16 16 363 0.0 3 0 354 9 17 52656 0.0 3 51531 1095 28 2 18 114 0.0 3 7 104 3 19 22 0.0 3 3 0 18 1 20 9 0.0 3 0 0 0 9 21 2 0.0 3 2 23 4 0.0 3 1 0 0 3 28 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 41 20 0.0 3 0 0 0 20 44 6 0.0 3 0 0 0 6 50 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 1 1 75 2 0.0 3 0 0 0 2 76 4 0.0 3 0 0 0 4 77 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 13 0.0 3 0 0 0 13 109 2 0.0 3 0 0 0 2 112 3 0.0 3 0 0 0 3 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 133 3 0.0 3 0 0 0 3 134 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 138 1 0.0 3 0 1 141 2 0.0 3 0 0 0 2 142 2 0.0 3 0 0 0 2 146 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 155 1 0.0 3 1 159 1 0.0 3 0 0 1 165 3 0.0 3 0 0 0 3 170 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 1 178 1 0.0 3 0 0 0 1 207 8 0.0 3 0 0 0 8 208 4 0.0 3 0 0 0 4 209 13 0.0 3 0 0 1 12 210 9 0.0 3 0 0 0 9 218 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.86 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 53,257 Reads with adapters: 65 (0.1%) Reads written (passing filters): 53,257 (100.0%) Total basepairs processed: 14,740,684 bp Total written (filtered): 14,740,477 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 65 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.5% C: 49.2% G: 29.2% T: 3.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 54 832.1 0 54 4 10 208.0 0 10 5 1 52.0 0 1