This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.91 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 333,162 (13.1%) Reads that were too short: 6,989 (0.3%) Reads written (passing filters): 326,173 (12.8%) Total basepairs processed: 766,381,518 bp Total written (filtered): 88,242,358 bp (11.5%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 333162 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 10494 621.6 0 10494 7 76909 621.6 0 76909 8 4016 621.6 0 4016 9 91848 621.6 0 91848 10 116 621.6 0 116 11 42 621.6 0 42 12 25 621.6 0 25 13 34 621.6 0 34 14 34 621.6 0 34 15 35 621.6 0 35 16 19 621.6 0 19 17 57 621.6 0 57 18 34 621.6 0 34 19 70 621.6 0 70 20 78 621.6 0 78 21 66 621.6 0 66 22 56 621.6 0 56 23 94 621.6 0 94 24 216 621.6 0 216 25 62 621.6 0 62 26 67 621.6 0 67 27 585 621.6 0 585 28 188 621.6 0 188 29 103 621.6 0 103 30 434 621.6 0 434 31 26 621.6 0 26 32 30 621.6 0 30 33 106 621.6 0 106 34 149 621.6 0 149 35 252 621.6 0 252 36 252 621.6 0 252 37 826 621.6 0 826 38 102 621.6 0 102 39 259 621.6 0 259 40 127 621.6 0 127 41 186 621.6 0 186 42 735 621.6 0 735 43 448 621.6 0 448 44 353 621.6 0 353 45 90 621.6 0 90 46 104 621.6 0 104 47 290 621.6 0 290 48 329 621.6 0 329 49 578 621.6 0 578 50 910 621.6 0 910 51 812 621.6 0 812 52 2010 621.6 0 2010 53 2750 621.6 0 2750 54 16985 621.6 0 16985 55 59964 621.6 0 59964 56 41816 621.6 0 41816 57 192 621.6 0 192 58 45 621.6 0 45 59 32 621.6 0 32 60 34 621.6 0 34 61 19 621.6 0 19 62 19 621.6 0 19 63 20 621.6 0 20 64 16 621.6 0 16 65 11 621.6 0 11 66 15 621.6 0 15 67 17 621.6 0 17 68 36 621.6 0 36 69 30 621.6 0 30 70 21 621.6 0 21 71 22 621.6 0 22 72 20 621.6 0 20 73 26 621.6 0 26 74 19 621.6 0 19 75 20 621.6 0 20 76 14 621.6 0 14 77 25 621.6 0 25 78 18 621.6 0 18 79 25 621.6 0 25 80 18 621.6 0 18 81 52 621.6 0 52 82 33 621.6 0 33 83 25 621.6 0 25 84 33 621.6 0 33 85 26 621.6 0 26 86 11 621.6 0 11 87 26 621.6 0 26 88 22 621.6 0 22 89 23 621.6 0 23 90 26 621.6 0 26 91 26 621.6 0 26 92 35 621.6 0 35 93 43 621.6 0 43 94 269 621.6 0 269 95 464 621.6 0 464 96 712 621.6 0 712 97 12 621.6 0 12 98 21 621.6 0 21 99 18 621.6 0 18 100 19 621.6 0 19 101 25 621.6 0 25 102 24 621.6 0 24 103 17 621.6 0 17 104 28 621.6 0 28 105 33 621.6 0 33 106 7 621.6 0 7 107 25 621.6 0 25 108 35 621.6 0 35 109 297 621.6 0 297 110 286 621.6 0 286 111 474 621.6 0 474 112 15 621.6 0 15 113 13 621.6 0 13 114 11 621.6 0 11 115 22 621.6 0 22 116 26 621.6 0 26 117 37 621.6 0 37 118 45 621.6 0 45 119 102 621.6 0 102 120 128 621.6 0 128 121 424 621.6 0 424 122 148 621.6 0 148 123 140 621.6 0 140 124 18 621.6 0 18 125 25 621.6 0 25 126 31 621.6 0 31 127 26 621.6 0 26 128 19 621.6 0 19 129 27 621.6 0 27 130 45 621.6 0 45 131 25 621.6 0 25 132 21 621.6 0 21 133 38 621.6 0 38 134 29 621.6 0 29 135 94 621.6 0 94 136 286 621.6 0 286 137 90 621.6 0 90 138 76 621.6 0 76 139 72 621.6 0 72 140 84 621.6 0 84 141 210 621.6 0 210 142 36 621.6 0 36 143 27 621.6 0 27 144 32 621.6 0 32 145 61 621.6 0 61 146 31 621.6 0 31 147 63 621.6 0 63 148 114 621.6 0 114 149 73 621.6 0 73 150 61 621.6 0 61 151 36 621.6 0 36 152 35 621.6 0 35 153 36 621.6 0 36 154 28 621.6 0 28 155 30 621.6 0 30 156 42 621.6 0 42 157 47 621.6 0 47 158 32 621.6 0 32 159 43 621.6 0 43 160 101 621.6 0 101 161 132 621.6 0 132 162 197 621.6 0 197 163 106 621.6 0 106 164 34 621.6 0 34 165 236 621.6 0 236 166 222 621.6 0 222 167 170 621.6 0 170 168 71 621.6 0 71 169 35 621.6 0 35 170 26 621.6 0 26 171 30 621.6 0 30 172 32 621.6 0 32 173 42 621.6 0 42 174 45 621.6 0 45 175 126 621.6 0 126 176 133 621.6 0 133 177 100 621.6 0 100 178 85 621.6 0 85 179 44 621.6 0 44 180 117 621.6 0 117 181 88 621.6 0 88 182 225 621.6 0 225 183 36 621.6 0 36 184 22 621.6 0 22 185 49 621.6 0 49 186 32 621.6 0 32 187 29 621.6 0 29 188 55 621.6 0 55 189 19 621.6 0 19 190 18 621.6 0 18 191 36 621.6 0 36 192 35 621.6 0 35 193 34 621.6 0 34 194 39 621.6 0 39 195 25 621.6 0 25 196 26 621.6 0 26 197 25 621.6 0 25 198 29 621.6 0 29 199 33 621.6 0 33 200 28 621.6 0 28 201 20 621.6 0 20 202 33 621.6 0 33 203 55 621.6 0 55 204 61 621.6 0 61 205 63 621.6 0 63 206 38 621.6 0 38 207 39 621.6 0 39 208 44 621.6 0 44 209 50 621.6 0 50 210 49 621.6 0 49 211 43 621.6 0 43 212 30 621.6 0 30 213 43 621.6 0 43 214 40 621.6 0 40 215 44 621.6 0 44 216 33 621.6 0 33 217 34 621.6 0 34 218 38 621.6 0 38 219 44 621.6 0 44 220 43 621.6 0 43 221 40 621.6 0 40 222 49 621.6 0 49 223 101 621.6 0 101 224 87 621.6 0 87 225 70 621.6 0 70 226 50 621.6 0 50 227 43 621.6 0 43 228 59 621.6 0 59 229 88 621.6 0 88 230 85 621.6 0 85 231 50 621.6 0 50 232 41 621.6 0 41 233 27 621.6 0 27 234 20 621.6 0 20 235 25 621.6 0 25 236 35 621.6 0 35 237 40 621.6 0 40 238 33 621.6 0 33 239 45 621.6 0 45 240 43 621.6 0 43 241 27 621.6 0 27 242 39 621.6 0 39 243 31 621.6 0 31 244 30 621.6 0 30 245 37 621.6 0 37 246 44 621.6 0 44 247 52 621.6 0 52 248 56 621.6 0 56 249 43 621.6 0 43 250 30 621.6 0 30 251 37 621.6 0 37 252 53 621.6 0 53 253 72 621.6 0 72 254 83 621.6 0 83 255 87 621.6 0 87 256 149 621.6 0 149 257 166 621.6 0 166 258 48 621.6 0 48 259 78 621.6 0 78 260 65 621.6 0 65 261 63 621.6 0 63 262 190 621.6 0 190 263 161 621.6 0 161 264 272 621.6 0 272 265 45 621.6 0 45 266 39 621.6 0 39 267 51 621.6 0 51 268 36 621.6 0 36 269 38 621.6 0 38 270 41 621.6 0 41 271 56 621.6 0 56 272 53 621.6 0 53 273 71 621.6 0 71 274 57 621.6 0 57 275 84 621.6 0 84 276 48 621.6 0 48 277 51 621.6 0 51 278 246 621.6 0 246 279 185 621.6 0 185 280 182 621.6 0 182 281 59 621.6 0 59 282 130 621.6 0 130 283 139 621.6 0 139 284 161 621.6 0 161 285 59 621.6 0 59 286 42 621.6 0 42 287 44 621.6 0 44 288 40 621.6 0 40 289 64 621.6 0 64 290 50 621.6 0 50 291 53 621.6 0 53 292 108 621.6 0 108 293 55 621.6 0 55 294 74 621.6 0 74 295 56 621.6 0 56 296 44 621.6 0 44 297 44 621.6 0 44 298 41 621.6 0 41 299 84 621.6 0 84 300 408 621.6 0 408 301 183 621.6 0 183 Finished in 15.61 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 326,173 Reads with adapters: 85,663 (26.3%) Reads that were too short: 72 (0.0%) Reads written (passing filters): 85,591 (26.2%) Total basepairs processed: 88,242,358 bp Total written (filtered): 23,431,974 bp (26.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 85663 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 9 0.0 3 0 0 0 5 4 18 47 0.0 3 0 0 33 12 2 19 1024 0.0 3 0 861 81 53 29 20 83784 0.0 4 78158 4548 810 204 64 21 206 0.0 4 20 167 12 4 3 22 28 0.0 4 1 1 17 4 5 23 224 0.0 4 0 1 1 160 62 24 3 0.0 4 0 0 0 0 3 25 1 0.0 4 1 26 1 0.0 4 1 28 2 0.0 4 0 0 0 0 2 35 2 0.0 4 1 1 45 1 0.0 4 1 50 2 0.0 4 0 0 0 0 2 51 1 0.0 4 1 52 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 1 101 2 0.0 4 0 0 0 0 2 125 2 0.0 4 0 0 0 0 2 126 26 0.0 4 0 0 0 0 26 127 215 0.0 4 0 0 0 0 215 129 6 0.0 4 0 0 0 0 6 130 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 169 9 0.0 4 0 0 0 0 9 173 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 208 2 0.0 4 0 0 0 0 2 213 1 0.0 4 0 0 0 1 214 3 0.0 4 0 0 0 0 3 215 2 0.0 4 0 0 0 0 2 224 2 0.0 4 0 0 0 1 1 228 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 3 0.0 4 0 0 0 0 3 234 8 0.0 4 0 0 0 2 6 235 3 0.0 4 0 0 0 0 3 237 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 265 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 10 0.0 4 0 0 0 0 10 283 2 0.0 4 0 0 0 1 1 284 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 288 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.08 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 85,591 Reads with adapters: 13,969 (16.3%) Reads written (passing filters): 85,591 (100.0%) Total basepairs processed: 23,431,974 bp Total written (filtered): 23,266,002 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 13969 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.0% C: 6.7% G: 88.4% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 349 1337.4 0 349 4 60 334.3 0 60 5 4 83.6 0 4 6 2 20.9 0 2 8 2 1.3 0 2 9 38 0.3 0 0 38 10 89 0.1 1 4 85 11 12167 0.0 1 248 11919 12 1120 0.0 1 25 1095 13 36 0.0 1 0 36 14 8 0.0 1 0 8 15 6 0.0 1 0 6 16 2 0.0 1 0 2 39 1 0.0 1 0 1 74 1 0.0 1 1 149 1 0.0 1 1 150 2 0.0 1 2 152 40 0.0 1 23 17 153 2 0.0 1 0 2 162 1 0.0 1 1 171 3 0.0 1 3 172 1 0.0 1 1 177 1 0.0 1 1 210 5 0.0 1 2 3 211 2 0.0 1 2 223 2 0.0 1 0 2 228 19 0.0 1 17 2 229 2 0.0 1 2 230 1 0.0 1 1 257 1 0.0 1 1 259 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.