This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.52 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 357,950 (14.1%) Reads that were too short: 4,766 (0.2%) Reads written (passing filters): 353,184 (13.9%) Total basepairs processed: 766,381,518 bp Total written (filtered): 95,617,555 bp (12.5%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 357950 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 9012 621.6 0 9012 7 97267 621.6 0 97267 8 3150 621.6 0 3150 9 83778 621.6 0 83778 10 86 621.6 0 86 11 38 621.6 0 38 12 25 621.6 0 25 13 26 621.6 0 26 14 42 621.6 0 42 15 49 621.6 0 49 16 26 621.6 0 26 17 53 621.6 0 53 18 53 621.6 0 53 19 77 621.6 0 77 20 98 621.6 0 98 21 72 621.6 0 72 22 55 621.6 0 55 23 111 621.6 0 111 24 272 621.6 0 272 25 86 621.6 0 86 26 68 621.6 0 68 27 723 621.6 0 723 28 61 621.6 0 61 29 54 621.6 0 54 30 65 621.6 0 65 31 45 621.6 0 45 32 31 621.6 0 31 33 59 621.6 0 59 34 133 621.6 0 133 35 167 621.6 0 167 36 326 621.6 0 326 37 924 621.6 0 924 38 127 621.6 0 127 39 286 621.6 0 286 40 168 621.6 0 168 41 196 621.6 0 196 42 935 621.6 0 935 43 558 621.6 0 558 44 464 621.6 0 464 45 111 621.6 0 111 46 128 621.6 0 128 47 264 621.6 0 264 48 331 621.6 0 331 49 535 621.6 0 535 50 827 621.6 0 827 51 770 621.6 0 770 52 2007 621.6 0 2007 53 2565 621.6 0 2565 54 19148 621.6 0 19148 55 69179 621.6 0 69179 56 50243 621.6 0 50243 57 225 621.6 0 225 58 54 621.6 0 54 59 29 621.6 0 29 60 38 621.6 0 38 61 30 621.6 0 30 62 25 621.6 0 25 63 28 621.6 0 28 64 18 621.6 0 18 65 21 621.6 0 21 66 27 621.6 0 27 67 18 621.6 0 18 68 20 621.6 0 20 69 43 621.6 0 43 70 32 621.6 0 32 71 26 621.6 0 26 72 33 621.6 0 33 73 30 621.6 0 30 74 32 621.6 0 32 75 30 621.6 0 30 76 24 621.6 0 24 77 24 621.6 0 24 78 22 621.6 0 22 79 9 621.6 0 9 80 34 621.6 0 34 81 46 621.6 0 46 82 34 621.6 0 34 83 31 621.6 0 31 84 51 621.6 0 51 85 36 621.6 0 36 86 35 621.6 0 35 87 34 621.6 0 34 88 34 621.6 0 34 89 36 621.6 0 36 90 18 621.6 0 18 91 30 621.6 0 30 92 46 621.6 0 46 93 31 621.6 0 31 94 198 621.6 0 198 95 370 621.6 0 370 96 695 621.6 0 695 97 25 621.6 0 25 98 36 621.6 0 36 99 29 621.6 0 29 100 37 621.6 0 37 101 32 621.6 0 32 102 28 621.6 0 28 103 29 621.6 0 29 104 25 621.6 0 25 105 30 621.6 0 30 106 22 621.6 0 22 107 30 621.6 0 30 108 36 621.6 0 36 109 91 621.6 0 91 110 149 621.6 0 149 111 150 621.6 0 150 112 21 621.6 0 21 113 29 621.6 0 29 114 25 621.6 0 25 115 26 621.6 0 26 116 32 621.6 0 32 117 41 621.6 0 41 118 49 621.6 0 49 119 52 621.6 0 52 120 81 621.6 0 81 121 189 621.6 0 189 122 111 621.6 0 111 123 67 621.6 0 67 124 26 621.6 0 26 125 45 621.6 0 45 126 36 621.6 0 36 127 16 621.6 0 16 128 23 621.6 0 23 129 33 621.6 0 33 130 34 621.6 0 34 131 28 621.6 0 28 132 25 621.6 0 25 133 31 621.6 0 31 134 33 621.6 0 33 135 51 621.6 0 51 136 72 621.6 0 72 137 44 621.6 0 44 138 34 621.6 0 34 139 27 621.6 0 27 140 52 621.6 0 52 141 94 621.6 0 94 142 40 621.6 0 40 143 36 621.6 0 36 144 35 621.6 0 35 145 59 621.6 0 59 146 29 621.6 0 29 147 49 621.6 0 49 148 43 621.6 0 43 149 43 621.6 0 43 150 28 621.6 0 28 151 31 621.6 0 31 152 35 621.6 0 35 153 47 621.6 0 47 154 44 621.6 0 44 155 35 621.6 0 35 156 36 621.6 0 36 157 23 621.6 0 23 158 30 621.6 0 30 159 42 621.6 0 42 160 48 621.6 0 48 161 54 621.6 0 54 162 61 621.6 0 61 163 36 621.6 0 36 164 35 621.6 0 35 165 51 621.6 0 51 166 55 621.6 0 55 167 60 621.6 0 60 168 35 621.6 0 35 169 32 621.6 0 32 170 23 621.6 0 23 171 33 621.6 0 33 172 27 621.6 0 27 173 39 621.6 0 39 174 45 621.6 0 45 175 63 621.6 0 63 176 65 621.6 0 65 177 69 621.6 0 69 178 54 621.6 0 54 179 27 621.6 0 27 180 59 621.6 0 59 181 56 621.6 0 56 182 88 621.6 0 88 183 37 621.6 0 37 184 30 621.6 0 30 185 52 621.6 0 52 186 31 621.6 0 31 187 28 621.6 0 28 188 72 621.6 0 72 189 35 621.6 0 35 190 30 621.6 0 30 191 27 621.6 0 27 192 51 621.6 0 51 193 41 621.6 0 41 194 49 621.6 0 49 195 36 621.6 0 36 196 41 621.6 0 41 197 41 621.6 0 41 198 35 621.6 0 35 199 46 621.6 0 46 200 29 621.6 0 29 201 26 621.6 0 26 202 23 621.6 0 23 203 57 621.6 0 57 204 49 621.6 0 49 205 52 621.6 0 52 206 41 621.6 0 41 207 37 621.6 0 37 208 40 621.6 0 40 209 26 621.6 0 26 210 20 621.6 0 20 211 35 621.6 0 35 212 43 621.6 0 43 213 32 621.6 0 32 214 25 621.6 0 25 215 28 621.6 0 28 216 32 621.6 0 32 217 38 621.6 0 38 218 31 621.6 0 31 219 37 621.6 0 37 220 33 621.6 0 33 221 32 621.6 0 32 222 44 621.6 0 44 223 53 621.6 0 53 224 37 621.6 0 37 225 57 621.6 0 57 226 34 621.6 0 34 227 38 621.6 0 38 228 61 621.6 0 61 229 43 621.6 0 43 230 39 621.6 0 39 231 34 621.6 0 34 232 29 621.6 0 29 233 35 621.6 0 35 234 26 621.6 0 26 235 29 621.6 0 29 236 37 621.6 0 37 237 38 621.6 0 38 238 27 621.6 0 27 239 29 621.6 0 29 240 36 621.6 0 36 241 36 621.6 0 36 242 33 621.6 0 33 243 26 621.6 0 26 244 31 621.6 0 31 245 16 621.6 0 16 246 25 621.6 0 25 247 43 621.6 0 43 248 36 621.6 0 36 249 35 621.6 0 35 250 34 621.6 0 34 251 15 621.6 0 15 252 43 621.6 0 43 253 33 621.6 0 33 254 50 621.6 0 50 255 41 621.6 0 41 256 55 621.6 0 55 257 129 621.6 0 129 258 40 621.6 0 40 259 56 621.6 0 56 260 42 621.6 0 42 261 37 621.6 0 37 262 71 621.6 0 71 263 55 621.6 0 55 264 71 621.6 0 71 265 41 621.6 0 41 266 39 621.6 0 39 267 35 621.6 0 35 268 24 621.6 0 24 269 45 621.6 0 45 270 26 621.6 0 26 271 53 621.6 0 53 272 54 621.6 0 54 273 62 621.6 0 62 274 39 621.6 0 39 275 87 621.6 0 87 276 47 621.6 0 47 277 31 621.6 0 31 278 110 621.6 0 110 279 85 621.6 0 85 280 76 621.6 0 76 281 36 621.6 0 36 282 45 621.6 0 45 283 45 621.6 0 45 284 72 621.6 0 72 285 34 621.6 0 34 286 24 621.6 0 24 287 38 621.6 0 38 288 30 621.6 0 30 289 35 621.6 0 35 290 34 621.6 0 34 291 55 621.6 0 55 292 41 621.6 0 41 293 55 621.6 0 55 294 47 621.6 0 47 295 49 621.6 0 49 296 53 621.6 0 53 297 35 621.6 0 35 298 44 621.6 0 44 299 75 621.6 0 75 300 437 621.6 0 437 301 138 621.6 0 138 Finished in 14.97 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 353,184 Reads with adapters: 88,087 (24.9%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 88,074 (24.9%) Total basepairs processed: 95,617,555 bp Total written (filtered): 24,212,569 bp (25.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 88087 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 20 0.0 3 0 0 19 1 16 580 0.0 3 0 567 13 17 86492 0.0 3 84767 1672 51 2 18 210 0.0 3 9 198 2 1 19 663 0.0 3 622 10 24 7 20 22 0.0 3 1 1 1 19 22 1 0.0 3 1 23 2 0.0 3 0 0 0 2 25 1 0.0 3 0 0 0 1 27 1 0.0 3 1 29 1 0.0 3 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 1 34 1 0.0 3 1 37 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 72 3 0.0 3 0 0 0 3 75 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 60 0.0 3 0 0 0 60 118 2 0.0 3 0 0 0 2 132 1 0.0 3 1 136 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 1 222 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 244 1 0.0 3 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.10 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 88,074 Reads with adapters: 941 (1.1%) Reads written (passing filters): 88,074 (100.0%) Total basepairs processed: 24,212,569 bp Total written (filtered): 24,209,643 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 941 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 46.0% C: 6.5% G: 46.5% T: 1.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 872 1376.2 0 872 4 62 344.0 0 62 7 1 5.4 0 1 9 5 0.3 0 0 5 10 1 0.1 1 0 1