This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.95 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 99,940 (3.9%) Reads that were too short: 16,898 (0.7%) Reads written (passing filters): 83,042 (3.3%) Total basepairs processed: 766,381,518 bp Total written (filtered): 20,596,023 bp (2.7%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 99940 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 364 621.6 0 364 7 537 621.6 0 537 8 22295 621.6 0 22295 9 24283 621.6 0 24283 10 89 621.6 0 89 11 107 621.6 0 107 12 108 621.6 0 108 13 94 621.6 0 94 14 122 621.6 0 122 15 73 621.6 0 73 16 96 621.6 0 96 17 96 621.6 0 96 18 75 621.6 0 75 19 69 621.6 0 69 20 85 621.6 0 85 21 89 621.6 0 89 22 86 621.6 0 86 23 85 621.6 0 85 24 99 621.6 0 99 25 84 621.6 0 84 26 100 621.6 0 100 27 96 621.6 0 96 28 95 621.6 0 95 29 109 621.6 0 109 30 72 621.6 0 72 31 97 621.6 0 97 32 105 621.6 0 105 33 65 621.6 0 65 34 96 621.6 0 96 35 121 621.6 0 121 36 83 621.6 0 83 37 86 621.6 0 86 38 159 621.6 0 159 39 94 621.6 0 94 40 85 621.6 0 85 41 112 621.6 0 112 42 85 621.6 0 85 43 89 621.6 0 89 44 104 621.6 0 104 45 150 621.6 0 150 46 104 621.6 0 104 47 132 621.6 0 132 48 90 621.6 0 90 49 111 621.6 0 111 50 88 621.6 0 88 51 91 621.6 0 91 52 79 621.6 0 79 53 70 621.6 0 70 54 83 621.6 0 83 55 88 621.6 0 88 56 109 621.6 0 109 57 108 621.6 0 108 58 74 621.6 0 74 59 93 621.6 0 93 60 114 621.6 0 114 61 85 621.6 0 85 62 117 621.6 0 117 63 102 621.6 0 102 64 100 621.6 0 100 65 127 621.6 0 127 66 157 621.6 0 157 67 126 621.6 0 126 68 148 621.6 0 148 69 148 621.6 0 148 70 134 621.6 0 134 71 127 621.6 0 127 72 122 621.6 0 122 73 110 621.6 0 110 74 110 621.6 0 110 75 146 621.6 0 146 76 161 621.6 0 161 77 170 621.6 0 170 78 208 621.6 0 208 79 1237 621.6 0 1237 80 1415 621.6 0 1415 81 3762 621.6 0 3762 82 208 621.6 0 208 83 206 621.6 0 206 84 235 621.6 0 235 85 157 621.6 0 157 86 135 621.6 0 135 87 168 621.6 0 168 88 190 621.6 0 190 89 159 621.6 0 159 90 131 621.6 0 131 91 162 621.6 0 162 92 170 621.6 0 170 93 135 621.6 0 135 94 109 621.6 0 109 95 120 621.6 0 120 96 176 621.6 0 176 97 148 621.6 0 148 98 172 621.6 0 172 99 162 621.6 0 162 100 154 621.6 0 154 101 151 621.6 0 151 102 168 621.6 0 168 103 157 621.6 0 157 104 157 621.6 0 157 105 163 621.6 0 163 106 152 621.6 0 152 107 170 621.6 0 170 108 198 621.6 0 198 109 189 621.6 0 189 110 143 621.6 0 143 111 175 621.6 0 175 112 172 621.6 0 172 113 145 621.6 0 145 114 142 621.6 0 142 115 164 621.6 0 164 116 191 621.6 0 191 117 150 621.6 0 150 118 138 621.6 0 138 119 160 621.6 0 160 120 150 621.6 0 150 121 148 621.6 0 148 122 137 621.6 0 137 123 129 621.6 0 129 124 208 621.6 0 208 125 282 621.6 0 282 126 256 621.6 0 256 127 310 621.6 0 310 128 242 621.6 0 242 129 196 621.6 0 196 130 262 621.6 0 262 131 141 621.6 0 141 132 149 621.6 0 149 133 128 621.6 0 128 134 138 621.6 0 138 135 161 621.6 0 161 136 188 621.6 0 188 137 172 621.6 0 172 138 178 621.6 0 178 139 320 621.6 0 320 140 208 621.6 0 208 141 208 621.6 0 208 142 183 621.6 0 183 143 256 621.6 0 256 144 175 621.6 0 175 145 129 621.6 0 129 146 192 621.6 0 192 147 141 621.6 0 141 148 109 621.6 0 109 149 115 621.6 0 115 150 253 621.6 0 253 151 219 621.6 0 219 152 218 621.6 0 218 153 124 621.6 0 124 154 154 621.6 0 154 155 132 621.6 0 132 156 150 621.6 0 150 157 134 621.6 0 134 158 198 621.6 0 198 159 235 621.6 0 235 160 249 621.6 0 249 161 141 621.6 0 141 162 151 621.6 0 151 163 145 621.6 0 145 164 295 621.6 0 295 165 145 621.6 0 145 166 187 621.6 0 187 167 154 621.6 0 154 168 121 621.6 0 121 169 142 621.6 0 142 170 162 621.6 0 162 171 143 621.6 0 143 172 128 621.6 0 128 173 129 621.6 0 129 174 133 621.6 0 133 175 146 621.6 0 146 176 144 621.6 0 144 177 185 621.6 0 185 178 177 621.6 0 177 179 144 621.6 0 144 180 139 621.6 0 139 181 198 621.6 0 198 182 744 621.6 0 744 183 517 621.6 0 517 184 1053 621.6 0 1053 185 151 621.6 0 151 186 98 621.6 0 98 187 139 621.6 0 139 188 122 621.6 0 122 189 152 621.6 0 152 190 121 621.6 0 121 191 145 621.6 0 145 192 147 621.6 0 147 193 124 621.6 0 124 194 135 621.6 0 135 195 140 621.6 0 140 196 131 621.6 0 131 197 154 621.6 0 154 198 137 621.6 0 137 199 136 621.6 0 136 200 196 621.6 0 196 201 147 621.6 0 147 202 114 621.6 0 114 203 160 621.6 0 160 204 142 621.6 0 142 205 160 621.6 0 160 206 151 621.6 0 151 207 162 621.6 0 162 208 243 621.6 0 243 209 183 621.6 0 183 210 161 621.6 0 161 211 143 621.6 0 143 212 171 621.6 0 171 213 152 621.6 0 152 214 130 621.6 0 130 215 176 621.6 0 176 216 142 621.6 0 142 217 137 621.6 0 137 218 187 621.6 0 187 219 159 621.6 0 159 220 183 621.6 0 183 221 140 621.6 0 140 222 179 621.6 0 179 223 151 621.6 0 151 224 155 621.6 0 155 225 221 621.6 0 221 226 399 621.6 0 399 227 457 621.6 0 457 228 257 621.6 0 257 229 148 621.6 0 148 230 122 621.6 0 122 231 248 621.6 0 248 232 441 621.6 0 441 233 223 621.6 0 223 234 101 621.6 0 101 235 183 621.6 0 183 236 144 621.6 0 144 237 141 621.6 0 141 238 165 621.6 0 165 239 148 621.6 0 148 240 133 621.6 0 133 241 172 621.6 0 172 242 141 621.6 0 141 243 144 621.6 0 144 244 107 621.6 0 107 245 130 621.6 0 130 246 157 621.6 0 157 247 156 621.6 0 156 248 178 621.6 0 178 249 158 621.6 0 158 250 143 621.6 0 143 251 131 621.6 0 131 252 127 621.6 0 127 253 253 621.6 0 253 254 450 621.6 0 450 255 749 621.6 0 749 256 340 621.6 0 340 257 183 621.6 0 183 258 123 621.6 0 123 259 139 621.6 0 139 260 150 621.6 0 150 261 153 621.6 0 153 262 142 621.6 0 142 263 153 621.6 0 153 264 257 621.6 0 257 265 192 621.6 0 192 266 188 621.6 0 188 267 116 621.6 0 116 268 137 621.6 0 137 269 133 621.6 0 133 270 132 621.6 0 132 271 123 621.6 0 123 272 132 621.6 0 132 273 125 621.6 0 125 274 128 621.6 0 128 275 154 621.6 0 154 276 136 621.6 0 136 277 156 621.6 0 156 278 133 621.6 0 133 279 104 621.6 0 104 280 145 621.6 0 145 281 132 621.6 0 132 282 121 621.6 0 121 283 112 621.6 0 112 284 166 621.6 0 166 285 130 621.6 0 130 286 127 621.6 0 127 287 121 621.6 0 121 288 113 621.6 0 113 289 121 621.6 0 121 290 125 621.6 0 125 291 87 621.6 0 87 292 105 621.6 0 105 293 116 621.6 0 116 294 127 621.6 0 127 295 129 621.6 0 129 296 138 621.6 0 138 297 137 621.6 0 137 298 92 621.6 0 92 299 141 621.6 0 141 300 126 621.6 0 126 301 150 621.6 0 150 Finished in 3.70 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 83,042 Reads with adapters: 21,843 (26.3%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 21,821 (26.3%) Total basepairs processed: 20,596,023 bp Total written (filtered): 6,015,822 bp (29.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 21843 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 2 0.0 3 0 0 2 16 121 0.0 3 0 117 4 17 21572 0.0 3 21190 371 10 1 18 48 0.0 3 6 42 19 20 0.0 3 9 0 11 20 7 0.0 3 1 0 0 6 21 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 28 5 0.0 3 0 0 0 5 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 7 0.0 3 0 0 0 7 44 6 0.0 3 0 0 0 6 49 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 78 1 0.0 3 1 90 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 112 16 0.0 3 0 0 0 16 115 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 172 3 0.0 3 0 0 0 3 204 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 214 3 0.0 3 0 0 0 3 278 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 291 1 0.0 3 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.76 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 21,821 Reads with adapters: 40 (0.2%) Reads written (passing filters): 21,821 (100.0%) Total basepairs processed: 6,015,822 bp Total written (filtered): 6,015,662 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 40 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 15.0% C: 0.0% G: 75.0% T: 10.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 341.0 0 4 4 33 85.2 0 33 5 2 21.3 0 2 6 1 5.3 0 1