This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.33 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 212,279 (8.3%) Reads that were too short: 11,306 (0.4%) Reads written (passing filters): 200,973 (7.9%) Total basepairs processed: 766,381,518 bp Total written (filtered): 57,280,340 bp (7.5%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 212279 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3204 621.6 0 3204 7 96952 621.6 0 96952 8 84829 621.6 0 84829 9 129 621.6 0 129 10 53 621.6 0 53 11 64 621.6 0 64 12 55 621.6 0 55 13 69 621.6 0 69 14 67 621.6 0 67 15 51 621.6 0 51 16 92 621.6 0 92 17 54 621.6 0 54 18 94 621.6 0 94 19 51 621.6 0 51 20 44 621.6 0 44 21 44 621.6 0 44 22 41 621.6 0 41 23 55 621.6 0 55 24 64 621.6 0 64 25 58 621.6 0 58 26 171 621.6 0 171 27 97 621.6 0 97 28 168 621.6 0 168 29 36 621.6 0 36 30 50 621.6 0 50 31 64 621.6 0 64 32 68 621.6 0 68 33 48 621.6 0 48 34 44 621.6 0 44 35 45 621.6 0 45 36 51 621.6 0 51 37 58 621.6 0 58 38 39 621.6 0 39 39 55 621.6 0 55 40 56 621.6 0 56 41 55 621.6 0 55 42 74 621.6 0 74 43 49 621.6 0 49 44 44 621.6 0 44 45 36 621.6 0 36 46 50 621.6 0 50 47 40 621.6 0 40 48 42 621.6 0 42 49 45 621.6 0 45 50 44 621.6 0 44 51 49 621.6 0 49 52 49 621.6 0 49 53 43 621.6 0 43 54 41 621.6 0 41 55 26 621.6 0 26 56 32 621.6 0 32 57 57 621.6 0 57 58 48 621.6 0 48 59 59 621.6 0 59 60 38 621.6 0 38 61 36 621.6 0 36 62 43 621.6 0 43 63 63 621.6 0 63 64 51 621.6 0 51 65 54 621.6 0 54 66 40 621.6 0 40 67 39 621.6 0 39 68 39 621.6 0 39 69 56 621.6 0 56 70 36 621.6 0 36 71 44 621.6 0 44 72 46 621.6 0 46 73 48 621.6 0 48 74 45 621.6 0 45 75 93 621.6 0 93 76 74 621.6 0 74 77 89 621.6 0 89 78 61 621.6 0 61 79 53 621.6 0 53 80 61 621.6 0 61 81 62 621.6 0 62 82 47 621.6 0 47 83 54 621.6 0 54 84 56 621.6 0 56 85 43 621.6 0 43 86 41 621.6 0 41 87 68 621.6 0 68 88 45 621.6 0 45 89 58 621.6 0 58 90 46 621.6 0 46 91 62 621.6 0 62 92 64 621.6 0 64 93 45 621.6 0 45 94 47 621.6 0 47 95 56 621.6 0 56 96 93 621.6 0 93 97 62 621.6 0 62 98 59 621.6 0 59 99 85 621.6 0 85 100 161 621.6 0 161 101 91 621.6 0 91 102 99 621.6 0 99 103 108 621.6 0 108 104 71 621.6 0 71 105 85 621.6 0 85 106 120 621.6 0 120 107 115 621.6 0 115 108 509 621.6 0 509 109 268 621.6 0 268 110 562 621.6 0 562 111 89 621.6 0 89 112 82 621.6 0 82 113 106 621.6 0 106 114 147 621.6 0 147 115 117 621.6 0 117 116 147 621.6 0 147 117 83 621.6 0 83 118 80 621.6 0 80 119 86 621.6 0 86 120 66 621.6 0 66 121 78 621.6 0 78 122 97 621.6 0 97 123 112 621.6 0 112 124 69 621.6 0 69 125 69 621.6 0 69 126 66 621.6 0 66 127 59 621.6 0 59 128 107 621.6 0 107 129 102 621.6 0 102 130 99 621.6 0 99 131 123 621.6 0 123 132 89 621.6 0 89 133 96 621.6 0 96 134 97 621.6 0 97 135 101 621.6 0 101 136 145 621.6 0 145 137 93 621.6 0 93 138 103 621.6 0 103 139 86 621.6 0 86 140 86 621.6 0 86 141 110 621.6 0 110 142 105 621.6 0 105 143 121 621.6 0 121 144 110 621.6 0 110 145 104 621.6 0 104 146 98 621.6 0 98 147 75 621.6 0 75 148 79 621.6 0 79 149 78 621.6 0 78 150 75 621.6 0 75 151 62 621.6 0 62 152 62 621.6 0 62 153 79 621.6 0 79 154 86 621.6 0 86 155 139 621.6 0 139 156 70 621.6 0 70 157 177 621.6 0 177 158 92 621.6 0 92 159 120 621.6 0 120 160 75 621.6 0 75 161 88 621.6 0 88 162 105 621.6 0 105 163 121 621.6 0 121 164 135 621.6 0 135 165 154 621.6 0 154 166 140 621.6 0 140 167 117 621.6 0 117 168 74 621.6 0 74 169 104 621.6 0 104 170 85 621.6 0 85 171 59 621.6 0 59 172 90 621.6 0 90 173 96 621.6 0 96 174 59 621.6 0 59 175 89 621.6 0 89 176 104 621.6 0 104 177 72 621.6 0 72 178 107 621.6 0 107 179 110 621.6 0 110 180 88 621.6 0 88 181 87 621.6 0 87 182 105 621.6 0 105 183 97 621.6 0 97 184 97 621.6 0 97 185 87 621.6 0 87 186 85 621.6 0 85 187 62 621.6 0 62 188 89 621.6 0 89 189 72 621.6 0 72 190 109 621.6 0 109 191 82 621.6 0 82 192 79 621.6 0 79 193 65 621.6 0 65 194 73 621.6 0 73 195 70 621.6 0 70 196 73 621.6 0 73 197 81 621.6 0 81 198 78 621.6 0 78 199 82 621.6 0 82 200 107 621.6 0 107 201 105 621.6 0 105 202 123 621.6 0 123 203 116 621.6 0 116 204 94 621.6 0 94 205 98 621.6 0 98 206 93 621.6 0 93 207 91 621.6 0 91 208 173 621.6 0 173 209 133 621.6 0 133 210 107 621.6 0 107 211 105 621.6 0 105 212 81 621.6 0 81 213 91 621.6 0 91 214 84 621.6 0 84 215 79 621.6 0 79 216 114 621.6 0 114 217 96 621.6 0 96 218 80 621.6 0 80 219 81 621.6 0 81 220 88 621.6 0 88 221 102 621.6 0 102 222 151 621.6 0 151 223 197 621.6 0 197 224 192 621.6 0 192 225 105 621.6 0 105 226 100 621.6 0 100 227 79 621.6 0 79 228 68 621.6 0 68 229 74 621.6 0 74 230 61 621.6 0 61 231 70 621.6 0 70 232 96 621.6 0 96 233 48 621.6 0 48 234 69 621.6 0 69 235 83 621.6 0 83 236 68 621.6 0 68 237 90 621.6 0 90 238 81 621.6 0 81 239 80 621.6 0 80 240 94 621.6 0 94 241 80 621.6 0 80 242 90 621.6 0 90 243 138 621.6 0 138 244 186 621.6 0 186 245 125 621.6 0 125 246 160 621.6 0 160 247 86 621.6 0 86 248 64 621.6 0 64 249 87 621.6 0 87 250 70 621.6 0 70 251 80 621.6 0 80 252 140 621.6 0 140 253 334 621.6 0 334 254 257 621.6 0 257 255 555 621.6 0 555 256 82 621.6 0 82 257 73 621.6 0 73 258 99 621.6 0 99 259 102 621.6 0 102 260 121 621.6 0 121 261 79 621.6 0 79 262 122 621.6 0 122 263 244 621.6 0 244 264 217 621.6 0 217 265 234 621.6 0 234 266 89 621.6 0 89 267 62 621.6 0 62 268 53 621.6 0 53 269 86 621.6 0 86 270 82 621.6 0 82 271 185 621.6 0 185 272 104 621.6 0 104 273 136 621.6 0 136 274 76 621.6 0 76 275 80 621.6 0 80 276 124 621.6 0 124 277 79 621.6 0 79 278 70 621.6 0 70 279 70 621.6 0 70 280 88 621.6 0 88 281 107 621.6 0 107 282 97 621.6 0 97 283 120 621.6 0 120 284 145 621.6 0 145 285 167 621.6 0 167 286 101 621.6 0 101 287 89 621.6 0 89 288 65 621.6 0 65 289 94 621.6 0 94 290 132 621.6 0 132 291 180 621.6 0 180 292 187 621.6 0 187 293 84 621.6 0 84 294 93 621.6 0 93 295 63 621.6 0 63 296 86 621.6 0 86 297 82 621.6 0 82 298 91 621.6 0 91 299 99 621.6 0 99 300 93 621.6 0 93 301 87 621.6 0 87 Finished in 10.37 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 200,973 Reads with adapters: 87,145 (43.4%) Reads that were too short: 71 (0.0%) Reads written (passing filters): 87,074 (43.3%) Total basepairs processed: 57,280,340 bp Total written (filtered): 23,754,708 bp (41.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 87145 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 10 0.0 3 0 0 0 5 5 18 40 0.0 3 0 0 23 11 6 19 842 0.0 3 0 608 96 101 37 20 85328 0.0 4 78874 5029 1019 305 101 21 245 0.0 4 34 183 18 4 6 22 41 0.0 4 11 4 20 3 3 23 377 0.0 4 1 0 1 247 128 24 6 0.0 4 1 0 0 0 5 25 4 0.0 4 1 0 0 1 2 26 11 0.0 4 3 0 0 0 8 27 3 0.0 4 2 0 0 0 1 29 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 94 1 0.0 4 1 107 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 148 0.0 4 0 0 0 0 148 129 4 0.0 4 0 0 0 0 4 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 156 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 1 205 2 0.0 4 1 0 0 0 1 208 3 0.0 4 0 0 0 0 3 213 1 0.0 4 0 0 0 1 215 1 0.0 4 0 0 0 1 225 2 0.0 4 0 0 0 0 2 228 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 0 2 234 8 0.0 4 0 0 0 0 8 235 5 0.0 4 0 0 0 1 4 236 1 0.0 4 0 0 0 0 1 237 3 0.0 4 0 0 0 0 3 253 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 1 1 259 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 282 11 0.0 4 0 0 0 0 11 283 3 0.0 4 0 0 0 1 2 284 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 288 4 0.0 4 0 0 0 0 4 291 2 0.0 4 0 0 0 1 1 293 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.15 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 87,074 Reads with adapters: 19,755 (22.7%) Reads written (passing filters): 87,074 (100.0%) Total basepairs processed: 23,754,708 bp Total written (filtered): 23,531,348 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 19755 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.1% C: 8.1% G: 87.6% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 115 1360.5 0 115 4 26 340.1 0 26 5 3 85.0 0 3 6 2 21.3 0 2 7 7 5.3 0 7 8 4 1.3 0 4 9 127 0.3 0 8 119 10 8735 0.1 1 502 8233 11 10516 0.0 1 341 10175 12 80 0.0 1 0 80 13 34 0.0 1 0 34 14 10 0.0 1 0 10 15 4 0.0 1 0 4 112 1 0.0 1 0 1 123 1 0.0 1 1 148 2 0.0 1 2 151 37 0.0 1 24 13 152 2 0.0 1 1 1 170 2 0.0 1 2 183 1 0.0 1 1 190 1 0.0 1 0 1 204 1 0.0 1 1 209 25 0.0 1 21 4 221 1 0.0 1 0 1 222 1 0.0 1 1 227 5 0.0 1 5 229 6 0.0 1 6 243 1 0.0 1 1 252 2 0.0 1 2 253 1 0.0 1 1 258 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.