This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.56 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 211,632 (8.3%) Reads that were too short: 7,504 (0.3%) Reads written (passing filters): 204,128 (8.0%) Total basepairs processed: 766,381,518 bp Total written (filtered): 58,791,692 bp (7.7%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 211632 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1837 621.6 0 1837 7 92615 621.6 0 92615 8 99804 621.6 0 99804 9 73 621.6 0 73 10 50 621.6 0 50 11 30 621.6 0 30 12 28 621.6 0 28 13 44 621.6 0 44 14 23 621.6 0 23 15 28 621.6 0 28 16 43 621.6 0 43 17 42 621.6 0 42 18 37 621.6 0 37 19 26 621.6 0 26 20 26 621.6 0 26 21 22 621.6 0 22 22 28 621.6 0 28 23 27 621.6 0 27 24 27 621.6 0 27 25 26 621.6 0 26 26 21 621.6 0 21 27 26 621.6 0 26 28 26 621.6 0 26 29 24 621.6 0 24 30 22 621.6 0 22 31 33 621.6 0 33 32 22 621.6 0 22 33 11 621.6 0 11 34 27 621.6 0 27 35 30 621.6 0 30 36 28 621.6 0 28 37 30 621.6 0 30 38 29 621.6 0 29 39 35 621.6 0 35 40 32 621.6 0 32 41 31 621.6 0 31 42 86 621.6 0 86 43 21 621.6 0 21 44 24 621.6 0 24 45 34 621.6 0 34 46 26 621.6 0 26 47 22 621.6 0 22 48 21 621.6 0 21 49 27 621.6 0 27 50 28 621.6 0 28 51 29 621.6 0 29 52 15 621.6 0 15 53 17 621.6 0 17 54 20 621.6 0 20 55 25 621.6 0 25 56 32 621.6 0 32 57 27 621.6 0 27 58 27 621.6 0 27 59 35 621.6 0 35 60 25 621.6 0 25 61 23 621.6 0 23 62 29 621.6 0 29 63 49 621.6 0 49 64 25 621.6 0 25 65 19 621.6 0 19 66 26 621.6 0 26 67 29 621.6 0 29 68 23 621.6 0 23 69 30 621.6 0 30 70 23 621.6 0 23 71 20 621.6 0 20 72 24 621.6 0 24 73 33 621.6 0 33 74 19 621.6 0 19 75 45 621.6 0 45 76 28 621.6 0 28 77 42 621.6 0 42 78 24 621.6 0 24 79 18 621.6 0 18 80 38 621.6 0 38 81 38 621.6 0 38 82 36 621.6 0 36 83 54 621.6 0 54 84 32 621.6 0 32 85 37 621.6 0 37 86 43 621.6 0 43 87 60 621.6 0 60 88 20 621.6 0 20 89 43 621.6 0 43 90 49 621.6 0 49 91 60 621.6 0 60 92 51 621.6 0 51 93 40 621.6 0 40 94 42 621.6 0 42 95 42 621.6 0 42 96 55 621.6 0 55 97 56 621.6 0 56 98 60 621.6 0 60 99 66 621.6 0 66 100 55 621.6 0 55 101 50 621.6 0 50 102 51 621.6 0 51 103 42 621.6 0 42 104 45 621.6 0 45 105 49 621.6 0 49 106 82 621.6 0 82 107 68 621.6 0 68 108 152 621.6 0 152 109 139 621.6 0 139 110 204 621.6 0 204 111 68 621.6 0 68 112 69 621.6 0 69 113 70 621.6 0 70 114 91 621.6 0 91 115 93 621.6 0 93 116 126 621.6 0 126 117 70 621.6 0 70 118 46 621.6 0 46 119 51 621.6 0 51 120 55 621.6 0 55 121 42 621.6 0 42 122 59 621.6 0 59 123 67 621.6 0 67 124 55 621.6 0 55 125 44 621.6 0 44 126 49 621.6 0 49 127 47 621.6 0 47 128 64 621.6 0 64 129 74 621.6 0 74 130 64 621.6 0 64 131 79 621.6 0 79 132 65 621.6 0 65 133 53 621.6 0 53 134 64 621.6 0 64 135 71 621.6 0 71 136 87 621.6 0 87 137 65 621.6 0 65 138 72 621.6 0 72 139 63 621.6 0 63 140 60 621.6 0 60 141 80 621.6 0 80 142 83 621.6 0 83 143 59 621.6 0 59 144 69 621.6 0 69 145 75 621.6 0 75 146 51 621.6 0 51 147 48 621.6 0 48 148 51 621.6 0 51 149 69 621.6 0 69 150 56 621.6 0 56 151 60 621.6 0 60 152 47 621.6 0 47 153 62 621.6 0 62 154 52 621.6 0 52 155 94 621.6 0 94 156 63 621.6 0 63 157 111 621.6 0 111 158 70 621.6 0 70 159 82 621.6 0 82 160 51 621.6 0 51 161 44 621.6 0 44 162 82 621.6 0 82 163 72 621.6 0 72 164 100 621.6 0 100 165 87 621.6 0 87 166 67 621.6 0 67 167 74 621.6 0 74 168 49 621.6 0 49 169 43 621.6 0 43 170 78 621.6 0 78 171 52 621.6 0 52 172 57 621.6 0 57 173 49 621.6 0 49 174 51 621.6 0 51 175 57 621.6 0 57 176 71 621.6 0 71 177 50 621.6 0 50 178 104 621.6 0 104 179 63 621.6 0 63 180 70 621.6 0 70 181 61 621.6 0 61 182 77 621.6 0 77 183 72 621.6 0 72 184 66 621.6 0 66 185 50 621.6 0 50 186 52 621.6 0 52 187 60 621.6 0 60 188 66 621.6 0 66 189 57 621.6 0 57 190 87 621.6 0 87 191 62 621.6 0 62 192 55 621.6 0 55 193 50 621.6 0 50 194 55 621.6 0 55 195 68 621.6 0 68 196 43 621.6 0 43 197 51 621.6 0 51 198 55 621.6 0 55 199 52 621.6 0 52 200 72 621.6 0 72 201 89 621.6 0 89 202 95 621.6 0 95 203 59 621.6 0 59 204 65 621.6 0 65 205 52 621.6 0 52 206 53 621.6 0 53 207 63 621.6 0 63 208 87 621.6 0 87 209 92 621.6 0 92 210 81 621.6 0 81 211 104 621.6 0 104 212 67 621.6 0 67 213 60 621.6 0 60 214 51 621.6 0 51 215 48 621.6 0 48 216 68 621.6 0 68 217 53 621.6 0 53 218 60 621.6 0 60 219 62 621.6 0 62 220 57 621.6 0 57 221 63 621.6 0 63 222 77 621.6 0 77 223 79 621.6 0 79 224 90 621.6 0 90 225 51 621.6 0 51 226 62 621.6 0 62 227 51 621.6 0 51 228 42 621.6 0 42 229 60 621.6 0 60 230 45 621.6 0 45 231 47 621.6 0 47 232 54 621.6 0 54 233 51 621.6 0 51 234 40 621.6 0 40 235 53 621.6 0 53 236 61 621.6 0 61 237 72 621.6 0 72 238 57 621.6 0 57 239 47 621.6 0 47 240 54 621.6 0 54 241 53 621.6 0 53 242 66 621.6 0 66 243 143 621.6 0 143 244 125 621.6 0 125 245 85 621.6 0 85 246 111 621.6 0 111 247 56 621.6 0 56 248 56 621.6 0 56 249 49 621.6 0 49 250 52 621.6 0 52 251 68 621.6 0 68 252 112 621.6 0 112 253 224 621.6 0 224 254 183 621.6 0 183 255 309 621.6 0 309 256 50 621.6 0 50 257 42 621.6 0 42 258 48 621.6 0 48 259 76 621.6 0 76 260 78 621.6 0 78 261 59 621.6 0 59 262 75 621.6 0 75 263 146 621.6 0 146 264 117 621.6 0 117 265 131 621.6 0 131 266 54 621.6 0 54 267 46 621.6 0 46 268 34 621.6 0 34 269 58 621.6 0 58 270 60 621.6 0 60 271 132 621.6 0 132 272 77 621.6 0 77 273 97 621.6 0 97 274 56 621.6 0 56 275 53 621.6 0 53 276 67 621.6 0 67 277 70 621.6 0 70 278 46 621.6 0 46 279 44 621.6 0 44 280 60 621.6 0 60 281 77 621.6 0 77 282 71 621.6 0 71 283 84 621.6 0 84 284 90 621.6 0 90 285 97 621.6 0 97 286 53 621.6 0 53 287 63 621.6 0 63 288 51 621.6 0 51 289 77 621.6 0 77 290 79 621.6 0 79 291 132 621.6 0 132 292 143 621.6 0 143 293 46 621.6 0 46 294 60 621.6 0 60 295 54 621.6 0 54 296 73 621.6 0 73 297 66 621.6 0 66 298 82 621.6 0 82 299 60 621.6 0 60 300 78 621.6 0 78 301 67 621.6 0 67 Finished in 9.40 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 204,128 Reads with adapters: 91,968 (45.1%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 91,948 (45.0%) Total basepairs processed: 58,791,692 bp Total written (filtered): 25,467,260 bp (43.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 91968 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 25 0.0 3 0 0 25 16 440 0.0 3 0 429 10 1 17 91223 0.0 3 89281 1890 45 7 18 177 0.0 3 20 152 4 1 19 34 0.0 3 0 0 33 1 20 24 0.0 3 0 0 0 24 23 1 0.0 3 0 0 0 1 25 2 0.0 3 0 0 0 2 26 2 0.0 3 2 27 2 0.0 3 1 0 0 1 29 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 60 6 0.0 3 0 0 0 6 63 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 217 5 0.0 3 0 0 0 5 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.21 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 91,948 Reads with adapters: 127 (0.1%) Reads written (passing filters): 91,948 (100.0%) Total basepairs processed: 25,467,260 bp Total written (filtered): 25,466,866 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 127 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.3% C: 51.2% G: 23.6% T: 3.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 119 1436.7 0 119 4 4 359.2 0 4 5 3 89.8 0 3 6 1 22.4 0 1