This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.43 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 240,689 (9.5%) Reads that were too short: 16,835 (0.7%) Reads written (passing filters): 223,854 (8.8%) Total basepairs processed: 766,381,518 bp Total written (filtered): 62,861,093 bp (8.2%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 240689 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6480 621.6 0 6480 7 50431 621.6 0 50431 8 5670 621.6 0 5670 9 135762 621.6 0 135762 10 186 621.6 0 186 11 104 621.6 0 104 12 102 621.6 0 102 13 126 621.6 0 126 14 116 621.6 0 116 15 88 621.6 0 88 16 76 621.6 0 76 17 101 621.6 0 101 18 115 621.6 0 115 19 94 621.6 0 94 20 86 621.6 0 86 21 104 621.6 0 104 22 95 621.6 0 95 23 83 621.6 0 83 24 99 621.6 0 99 25 75 621.6 0 75 26 84 621.6 0 84 27 86 621.6 0 86 28 81 621.6 0 81 29 94 621.6 0 94 30 83 621.6 0 83 31 71 621.6 0 71 32 75 621.6 0 75 33 96 621.6 0 96 34 114 621.6 0 114 35 88 621.6 0 88 36 101 621.6 0 101 37 112 621.6 0 112 38 95 621.6 0 95 39 86 621.6 0 86 40 90 621.6 0 90 41 88 621.6 0 88 42 109 621.6 0 109 43 75 621.6 0 75 44 100 621.6 0 100 45 97 621.6 0 97 46 126 621.6 0 126 47 154 621.6 0 154 48 163 621.6 0 163 49 176 621.6 0 176 50 131 621.6 0 131 51 88 621.6 0 88 52 111 621.6 0 111 53 101 621.6 0 101 54 145 621.6 0 145 55 122 621.6 0 122 56 100 621.6 0 100 57 94 621.6 0 94 58 113 621.6 0 113 59 146 621.6 0 146 60 120 621.6 0 120 61 158 621.6 0 158 62 109 621.6 0 109 63 99 621.6 0 99 64 67 621.6 0 67 65 78 621.6 0 78 66 98 621.6 0 98 67 80 621.6 0 80 68 105 621.6 0 105 69 124 621.6 0 124 70 91 621.6 0 91 71 98 621.6 0 98 72 112 621.6 0 112 73 102 621.6 0 102 74 82 621.6 0 82 75 93 621.6 0 93 76 114 621.6 0 114 77 158 621.6 0 158 78 134 621.6 0 134 79 114 621.6 0 114 80 86 621.6 0 86 81 104 621.6 0 104 82 90 621.6 0 90 83 113 621.6 0 113 84 259 621.6 0 259 85 179 621.6 0 179 86 243 621.6 0 243 87 76 621.6 0 76 88 79 621.6 0 79 89 79 621.6 0 79 90 78 621.6 0 78 91 105 621.6 0 105 92 87 621.6 0 87 93 93 621.6 0 93 94 114 621.6 0 114 95 93 621.6 0 93 96 88 621.6 0 88 97 60 621.6 0 60 98 72 621.6 0 72 99 86 621.6 0 86 100 73 621.6 0 73 101 76 621.6 0 76 102 99 621.6 0 99 103 79 621.6 0 79 104 60 621.6 0 60 105 81 621.6 0 81 106 108 621.6 0 108 107 93 621.6 0 93 108 103 621.6 0 103 109 91 621.6 0 91 110 109 621.6 0 109 111 75 621.6 0 75 112 272 621.6 0 272 113 315 621.6 0 315 114 292 621.6 0 292 115 165 621.6 0 165 116 89 621.6 0 89 117 107 621.6 0 107 118 105 621.6 0 105 119 79 621.6 0 79 120 96 621.6 0 96 121 89 621.6 0 89 122 79 621.6 0 79 123 94 621.6 0 94 124 81 621.6 0 81 125 112 621.6 0 112 126 96 621.6 0 96 127 144 621.6 0 144 128 161 621.6 0 161 129 143 621.6 0 143 130 163 621.6 0 163 131 130 621.6 0 130 132 212 621.6 0 212 133 224 621.6 0 224 134 301 621.6 0 301 135 222 621.6 0 222 136 179 621.6 0 179 137 150 621.6 0 150 138 104 621.6 0 104 139 117 621.6 0 117 140 182 621.6 0 182 141 2209 621.6 0 2209 142 414 621.6 0 414 143 1682 621.6 0 1682 144 85 621.6 0 85 145 79 621.6 0 79 146 82 621.6 0 82 147 103 621.6 0 103 148 103 621.6 0 103 149 101 621.6 0 101 150 84 621.6 0 84 151 122 621.6 0 122 152 190 621.6 0 190 153 254 621.6 0 254 154 259 621.6 0 259 155 142 621.6 0 142 156 99 621.6 0 99 157 100 621.6 0 100 158 91 621.6 0 91 159 100 621.6 0 100 160 90 621.6 0 90 161 92 621.6 0 92 162 100 621.6 0 100 163 90 621.6 0 90 164 104 621.6 0 104 165 118 621.6 0 118 166 110 621.6 0 110 167 85 621.6 0 85 168 81 621.6 0 81 169 103 621.6 0 103 170 83 621.6 0 83 171 87 621.6 0 87 172 89 621.6 0 89 173 126 621.6 0 126 174 87 621.6 0 87 175 105 621.6 0 105 176 84 621.6 0 84 177 55 621.6 0 55 178 91 621.6 0 91 179 82 621.6 0 82 180 87 621.6 0 87 181 81 621.6 0 81 182 87 621.6 0 87 183 88 621.6 0 88 184 145 621.6 0 145 185 95 621.6 0 95 186 146 621.6 0 146 187 167 621.6 0 167 188 86 621.6 0 86 189 82 621.6 0 82 190 104 621.6 0 104 191 105 621.6 0 105 192 96 621.6 0 96 193 113 621.6 0 113 194 68 621.6 0 68 195 97 621.6 0 97 196 109 621.6 0 109 197 76 621.6 0 76 198 76 621.6 0 76 199 106 621.6 0 106 200 83 621.6 0 83 201 138 621.6 0 138 202 221 621.6 0 221 203 204 621.6 0 204 204 84 621.6 0 84 205 90 621.6 0 90 206 86 621.6 0 86 207 88 621.6 0 88 208 53 621.6 0 53 209 78 621.6 0 78 210 66 621.6 0 66 211 90 621.6 0 90 212 98 621.6 0 98 213 133 621.6 0 133 214 126 621.6 0 126 215 99 621.6 0 99 216 86 621.6 0 86 217 105 621.6 0 105 218 89 621.6 0 89 219 91 621.6 0 91 220 83 621.6 0 83 221 74 621.6 0 74 222 55 621.6 0 55 223 63 621.6 0 63 224 74 621.6 0 74 225 94 621.6 0 94 226 131 621.6 0 131 227 92 621.6 0 92 228 183 621.6 0 183 229 91 621.6 0 91 230 116 621.6 0 116 231 96 621.6 0 96 232 107 621.6 0 107 233 82 621.6 0 82 234 85 621.6 0 85 235 301 621.6 0 301 236 221 621.6 0 221 237 363 621.6 0 363 238 144 621.6 0 144 239 140 621.6 0 140 240 80 621.6 0 80 241 67 621.6 0 67 242 68 621.6 0 68 243 91 621.6 0 91 244 74 621.6 0 74 245 105 621.6 0 105 246 116 621.6 0 116 247 99 621.6 0 99 248 115 621.6 0 115 249 69 621.6 0 69 250 88 621.6 0 88 251 68 621.6 0 68 252 178 621.6 0 178 253 111 621.6 0 111 254 96 621.6 0 96 255 188 621.6 0 188 256 356 621.6 0 356 257 325 621.6 0 325 258 172 621.6 0 172 259 57 621.6 0 57 260 134 621.6 0 134 261 195 621.6 0 195 262 147 621.6 0 147 263 79 621.6 0 79 264 80 621.6 0 80 265 75 621.6 0 75 266 63 621.6 0 63 267 76 621.6 0 76 268 84 621.6 0 84 269 63 621.6 0 63 270 93 621.6 0 93 271 72 621.6 0 72 272 125 621.6 0 125 273 462 621.6 0 462 274 306 621.6 0 306 275 427 621.6 0 427 276 102 621.6 0 102 277 42 621.6 0 42 278 73 621.6 0 73 279 80 621.6 0 80 280 88 621.6 0 88 281 69 621.6 0 69 282 115 621.6 0 115 283 124 621.6 0 124 284 131 621.6 0 131 285 175 621.6 0 175 286 145 621.6 0 145 287 225 621.6 0 225 288 161 621.6 0 161 289 108 621.6 0 108 290 97 621.6 0 97 291 109 621.6 0 109 292 124 621.6 0 124 293 126 621.6 0 126 294 132 621.6 0 132 295 135 621.6 0 135 296 153 621.6 0 153 297 221 621.6 0 221 298 1188 621.6 0 1188 299 1275 621.6 0 1275 300 1897 621.6 0 1897 301 254 621.6 0 254 Finished in 10.18 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 223,854 Reads with adapters: 48,114 (21.5%) Reads that were too short: 80 (0.0%) Reads written (passing filters): 48,034 (21.5%) Total basepairs processed: 62,861,093 bp Total written (filtered): 13,198,409 bp (21.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 48114 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 10 0.0 3 0 0 9 1 16 260 0.0 3 0 252 8 17 46528 0.0 3 45585 904 37 2 18 81 0.0 3 9 71 1 19 1043 0.0 3 980 31 25 7 20 10 0.0 3 0 4 0 6 21 1 0.0 3 1 28 1 0.0 3 1 39 8 0.0 3 0 0 0 8 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 50 38 0.0 3 0 0 1 37 51 3 0.0 3 0 0 0 3 53 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 79 4 0.0 3 0 0 0 4 82 2 0.0 3 0 0 0 2 85 1 0.0 3 0 1 88 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 101 59 0.0 3 0 0 0 59 102 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 143 8 0.0 3 0 0 0 8 150 1 0.0 3 0 0 1 151 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 1 0.0 3 1 179 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 192 2 0.0 3 0 0 0 2 199 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 1 276 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 4 0.0 3 0 0 0 4 281 5 0.0 3 0 0 1 4 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.68 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 48,034 Reads with adapters: 302 (0.6%) Reads written (passing filters): 48,034 (100.0%) Total basepairs processed: 13,198,409 bp Total written (filtered): 13,197,447 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 302 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 44.7% C: 6.0% G: 47.7% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 247 750.5 0 247 4 54 187.6 0 54 5 1 46.9 0 1