This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.99 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 353,515 (13.9%) Reads that were too short: 48,648 (1.9%) Reads written (passing filters): 304,867 (12.0%) Total basepairs processed: 766,381,518 bp Total written (filtered): 87,633,857 bp (11.4%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 353515 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4313 621.6 0 4313 7 152005 621.6 0 152005 8 2980 621.6 0 2980 9 131808 621.6 0 131808 10 268 621.6 0 268 11 57 621.6 0 57 12 51 621.6 0 51 13 27 621.6 0 27 14 25 621.6 0 25 15 31 621.6 0 31 16 40 621.6 0 40 17 18 621.6 0 18 18 20 621.6 0 20 19 25 621.6 0 25 20 19 621.6 0 19 21 29 621.6 0 29 22 20 621.6 0 20 23 25 621.6 0 25 24 32 621.6 0 32 25 27 621.6 0 27 26 27 621.6 0 27 27 23 621.6 0 23 28 21 621.6 0 21 29 21 621.6 0 21 30 33 621.6 0 33 31 24 621.6 0 24 32 35 621.6 0 35 33 29 621.6 0 29 34 17 621.6 0 17 35 18 621.6 0 18 36 16 621.6 0 16 37 22 621.6 0 22 38 17 621.6 0 17 39 19 621.6 0 19 40 25 621.6 0 25 41 33 621.6 0 33 42 36 621.6 0 36 43 23 621.6 0 23 44 27 621.6 0 27 45 25 621.6 0 25 46 22 621.6 0 22 47 16 621.6 0 16 48 20 621.6 0 20 49 17 621.6 0 17 50 25 621.6 0 25 51 9 621.6 0 9 52 23 621.6 0 23 53 36 621.6 0 36 54 34 621.6 0 34 55 79 621.6 0 79 56 56 621.6 0 56 57 44 621.6 0 44 58 23 621.6 0 23 59 36 621.6 0 36 60 24 621.6 0 24 61 50 621.6 0 50 62 38 621.6 0 38 63 30 621.6 0 30 64 30 621.6 0 30 65 26 621.6 0 26 66 20 621.6 0 20 67 45 621.6 0 45 68 30 621.6 0 30 69 48 621.6 0 48 70 163 621.6 0 163 71 44 621.6 0 44 72 48 621.6 0 48 73 55 621.6 0 55 74 109 621.6 0 109 75 78 621.6 0 78 76 108 621.6 0 108 77 33 621.6 0 33 78 31 621.6 0 31 79 54 621.6 0 54 80 53 621.6 0 53 81 146 621.6 0 146 82 249 621.6 0 249 83 209 621.6 0 209 84 363 621.6 0 363 85 96 621.6 0 96 86 99 621.6 0 99 87 42 621.6 0 42 88 40 621.6 0 40 89 50 621.6 0 50 90 35 621.6 0 35 91 45 621.6 0 45 92 44 621.6 0 44 93 31 621.6 0 31 94 34 621.6 0 34 95 30 621.6 0 30 96 28 621.6 0 28 97 29 621.6 0 29 98 32 621.6 0 32 99 20 621.6 0 20 100 33 621.6 0 33 101 27 621.6 0 27 102 28 621.6 0 28 103 34 621.6 0 34 104 51 621.6 0 51 105 37 621.6 0 37 106 41 621.6 0 41 107 35 621.6 0 35 108 32 621.6 0 32 109 33 621.6 0 33 110 41 621.6 0 41 111 33 621.6 0 33 112 57 621.6 0 57 113 54 621.6 0 54 114 38 621.6 0 38 115 37 621.6 0 37 116 45 621.6 0 45 117 26 621.6 0 26 118 26 621.6 0 26 119 49 621.6 0 49 120 31 621.6 0 31 121 68 621.6 0 68 122 113 621.6 0 113 123 345 621.6 0 345 124 56 621.6 0 56 125 83 621.6 0 83 126 45 621.6 0 45 127 56 621.6 0 56 128 45 621.6 0 45 129 35 621.6 0 35 130 30 621.6 0 30 131 26 621.6 0 26 132 41 621.6 0 41 133 72 621.6 0 72 134 42 621.6 0 42 135 45 621.6 0 45 136 67 621.6 0 67 137 43 621.6 0 43 138 29 621.6 0 29 139 60 621.6 0 60 140 56 621.6 0 56 141 36 621.6 0 36 142 31 621.6 0 31 143 37 621.6 0 37 144 27 621.6 0 27 145 47 621.6 0 47 146 41 621.6 0 41 147 50 621.6 0 50 148 54 621.6 0 54 149 33 621.6 0 33 150 45 621.6 0 45 151 40 621.6 0 40 152 60 621.6 0 60 153 54 621.6 0 54 154 61 621.6 0 61 155 36 621.6 0 36 156 33 621.6 0 33 157 52 621.6 0 52 158 27 621.6 0 27 159 39 621.6 0 39 160 34 621.6 0 34 161 35 621.6 0 35 162 43 621.6 0 43 163 53 621.6 0 53 164 45 621.6 0 45 165 55 621.6 0 55 166 86 621.6 0 86 167 608 621.6 0 608 168 378 621.6 0 378 169 581 621.6 0 581 170 87 621.6 0 87 171 274 621.6 0 274 172 159 621.6 0 159 173 142 621.6 0 142 174 38 621.6 0 38 175 47 621.6 0 47 176 38 621.6 0 38 177 30 621.6 0 30 178 29 621.6 0 29 179 34 621.6 0 34 180 96 621.6 0 96 181 64 621.6 0 64 182 104 621.6 0 104 183 163 621.6 0 163 184 237 621.6 0 237 185 64 621.6 0 64 186 67 621.6 0 67 187 53 621.6 0 53 188 105 621.6 0 105 189 123 621.6 0 123 190 79 621.6 0 79 191 77 621.6 0 77 192 41 621.6 0 41 193 50 621.6 0 50 194 72 621.6 0 72 195 67 621.6 0 67 196 68 621.6 0 68 197 81 621.6 0 81 198 109 621.6 0 109 199 175 621.6 0 175 200 717 621.6 0 717 201 1021 621.6 0 1021 202 1100 621.6 0 1100 203 389 621.6 0 389 204 43 621.6 0 43 205 105 621.6 0 105 206 107 621.6 0 107 207 93 621.6 0 93 208 45 621.6 0 45 209 45 621.6 0 45 210 79 621.6 0 79 211 163 621.6 0 163 212 71 621.6 0 71 213 68 621.6 0 68 214 132 621.6 0 132 215 131 621.6 0 131 216 147 621.6 0 147 217 123 621.6 0 123 218 122 621.6 0 122 219 154 621.6 0 154 220 122 621.6 0 122 221 164 621.6 0 164 222 102 621.6 0 102 223 209 621.6 0 209 224 358 621.6 0 358 225 332 621.6 0 332 226 562 621.6 0 562 227 1037 621.6 0 1037 228 6349 621.6 0 6349 229 2474 621.6 0 2474 230 20156 621.6 0 20156 231 266 621.6 0 266 232 79 621.6 0 79 233 74 621.6 0 74 234 77 621.6 0 77 235 74 621.6 0 74 236 81 621.6 0 81 237 84 621.6 0 84 238 99 621.6 0 99 239 92 621.6 0 92 240 87 621.6 0 87 241 76 621.6 0 76 242 65 621.6 0 65 243 76 621.6 0 76 244 97 621.6 0 97 245 145 621.6 0 145 246 207 621.6 0 207 247 243 621.6 0 243 248 251 621.6 0 251 249 189 621.6 0 189 250 108 621.6 0 108 251 89 621.6 0 89 252 69 621.6 0 69 253 63 621.6 0 63 254 61 621.6 0 61 255 133 621.6 0 133 256 293 621.6 0 293 257 227 621.6 0 227 258 289 621.6 0 289 259 128 621.6 0 128 260 151 621.6 0 151 261 653 621.6 0 653 262 669 621.6 0 669 263 965 621.6 0 965 264 278 621.6 0 278 265 361 621.6 0 361 266 228 621.6 0 228 267 183 621.6 0 183 268 626 621.6 0 626 269 619 621.6 0 619 270 503 621.6 0 503 271 133 621.6 0 133 272 94 621.6 0 94 273 83 621.6 0 83 274 118 621.6 0 118 275 100 621.6 0 100 276 117 621.6 0 117 277 84 621.6 0 84 278 82 621.6 0 82 279 69 621.6 0 69 280 109 621.6 0 109 281 173 621.6 0 173 282 184 621.6 0 184 283 210 621.6 0 210 284 102 621.6 0 102 285 62 621.6 0 62 286 91 621.6 0 91 287 126 621.6 0 126 288 145 621.6 0 145 289 109 621.6 0 109 290 66 621.6 0 66 291 86 621.6 0 86 292 249 621.6 0 249 293 269 621.6 0 269 294 367 621.6 0 367 295 111 621.6 0 111 296 78 621.6 0 78 297 69 621.6 0 69 298 80 621.6 0 80 299 161 621.6 0 161 300 381 621.6 0 381 301 500 621.6 0 500 Finished in 14.99 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 304,867 Reads with adapters: 138,885 (45.6%) Reads that were too short: 118 (0.0%) Reads written (passing filters): 138,767 (45.5%) Total basepairs processed: 87,633,857 bp Total written (filtered): 37,547,972 bp (42.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 138885 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 21 0.0 3 0 0 0 12 9 18 69 0.0 3 0 0 46 20 3 19 1377 0.0 3 0 1142 118 86 31 20 134563 0.0 4 125520 7076 1392 454 121 21 337 0.0 4 62 249 19 3 4 22 43 0.0 4 5 4 20 8 6 23 397 0.0 4 1 0 0 247 149 24 5 0.0 4 2 0 0 2 1 25 1 0.0 4 1 26 1 0.0 4 0 0 0 1 27 1 0.0 4 0 0 0 1 28 2 0.0 4 0 0 0 0 2 30 1 0.0 4 1 35 1 0.0 4 1 39 1 0.0 4 1 42 1 0.0 4 1 45 1 0.0 4 1 47 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 1 55 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 92 3 0.0 4 0 0 0 0 3 93 2 0.0 4 0 0 0 0 2 94 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 1 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 40 0.0 4 0 0 0 0 40 127 1874 0.0 4 0 0 0 0 1874 128 4 0.0 4 0 0 0 0 4 130 8 0.0 4 0 0 0 1 7 132 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 1 165 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 188 1 0.0 4 1 195 1 0.0 4 0 0 0 1 204 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 2 0.0 4 0 0 0 0 2 209 3 0.0 4 0 0 0 0 3 213 2 0.0 4 0 0 0 0 2 214 2 0.0 4 0 0 0 0 2 215 1 0.0 4 0 0 0 0 1 221 4 0.0 4 0 0 0 0 4 223 1 0.0 4 0 0 0 0 1 225 4 0.0 4 0 0 0 0 4 226 3 0.0 4 0 0 0 0 3 228 28 0.0 4 0 0 0 1 27 229 7 0.0 4 0 0 1 2 4 230 2 0.0 4 0 0 0 0 2 232 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 0 2 234 4 0.0 4 0 0 0 0 4 235 6 0.0 4 0 0 0 0 6 236 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 250 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 265 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 282 6 0.0 4 0 0 0 0 6 283 3 0.0 4 0 0 0 0 3 284 1 0.0 4 0 0 0 0 1 285 5 0.0 4 0 0 0 0 5 288 4 0.0 4 0 0 0 0 4 290 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.08 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 138,767 Reads with adapters: 35,802 (25.8%) Reads written (passing filters): 138,767 (100.0%) Total basepairs processed: 37,547,972 bp Total written (filtered): 37,160,054 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 35802 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.2% C: 7.5% G: 88.0% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 138 2168.2 0 138 4 42 542.1 0 42 5 4 135.5 0 4 6 25 33.9 0 25 7 8 8.5 0 8 8 4 2.1 0 4 9 9500 0.5 0 699 8801 10 18700 0.1 1 1203 17497 11 7036 0.0 1 229 6807 12 107 0.0 1 1 106 13 51 0.0 1 0 51 14 2 0.0 1 0 2 17 1 0.0 1 0 1 22 4 0.0 1 4 40 3 0.0 1 2 1 41 1 0.0 1 1 135 2 0.0 1 1 1 137 2 0.0 1 1 1 138 2 0.0 1 2 147 2 0.0 1 1 1 148 1 0.0 1 1 150 23 0.0 1 14 9 151 1 0.0 1 1 152 1 0.0 1 1 153 1 0.0 1 1 154 1 0.0 1 1 158 1 0.0 1 0 1 160 1 0.0 1 1 164 1 0.0 1 1 167 1 0.0 1 1 169 12 0.0 1 9 3 170 1 0.0 1 1 177 2 0.0 1 0 2 190 1 0.0 1 0 1 207 1 0.0 1 1 208 78 0.0 1 72 6 209 2 0.0 1 2 210 3 0.0 1 3 220 1 0.0 1 0 1 226 8 0.0 1 7 1 227 2 0.0 1 1 1 228 7 0.0 1 7 229 1 0.0 1 1 230 1 0.0 1 1 246 1 0.0 1 0 1 257 6 0.0 1 5 1 258 1 0.0 1 1 270 2 0.0 1 2 271 6 0.0 1 4 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.