This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.97 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 376,562 (14.8%) Reads that were too short: 57,155 (2.2%) Reads written (passing filters): 319,407 (12.5%) Total basepairs processed: 766,381,518 bp Total written (filtered): 91,892,326 bp (12.0%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 376562 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2154 621.6 0 2154 7 141067 621.6 0 141067 8 2263 621.6 0 2263 9 161448 621.6 0 161448 10 162 621.6 0 162 11 33 621.6 0 33 12 26 621.6 0 26 13 20 621.6 0 20 14 12 621.6 0 12 15 12 621.6 0 12 16 25 621.6 0 25 17 15 621.6 0 15 18 14 621.6 0 14 19 14 621.6 0 14 20 17 621.6 0 17 21 16 621.6 0 16 22 11 621.6 0 11 23 13 621.6 0 13 24 9 621.6 0 9 25 16 621.6 0 16 26 18 621.6 0 18 27 12 621.6 0 12 28 22 621.6 0 22 29 21 621.6 0 21 30 13 621.6 0 13 31 10 621.6 0 10 32 14 621.6 0 14 33 22 621.6 0 22 34 16 621.6 0 16 35 10 621.6 0 10 36 31 621.6 0 31 37 36 621.6 0 36 38 16 621.6 0 16 39 16 621.6 0 16 40 15 621.6 0 15 41 14 621.6 0 14 42 17 621.6 0 17 43 21 621.6 0 21 44 14 621.6 0 14 45 16 621.6 0 16 46 17 621.6 0 17 47 20 621.6 0 20 48 15 621.6 0 15 49 15 621.6 0 15 50 12 621.6 0 12 51 15 621.6 0 15 52 23 621.6 0 23 53 23 621.6 0 23 54 17 621.6 0 17 55 21 621.6 0 21 56 22 621.6 0 22 57 26 621.6 0 26 58 16 621.6 0 16 59 27 621.6 0 27 60 15 621.6 0 15 61 29 621.6 0 29 62 25 621.6 0 25 63 28 621.6 0 28 64 24 621.6 0 24 65 21 621.6 0 21 66 18 621.6 0 18 67 31 621.6 0 31 68 23 621.6 0 23 69 37 621.6 0 37 70 152 621.6 0 152 71 23 621.6 0 23 72 40 621.6 0 40 73 31 621.6 0 31 74 110 621.6 0 110 75 61 621.6 0 61 76 94 621.6 0 94 77 22 621.6 0 22 78 22 621.6 0 22 79 33 621.6 0 33 80 35 621.6 0 35 81 64 621.6 0 64 82 214 621.6 0 214 83 112 621.6 0 112 84 279 621.6 0 279 85 36 621.6 0 36 86 42 621.6 0 42 87 27 621.6 0 27 88 26 621.6 0 26 89 29 621.6 0 29 90 24 621.6 0 24 91 25 621.6 0 25 92 39 621.6 0 39 93 23 621.6 0 23 94 24 621.6 0 24 95 31 621.6 0 31 96 30 621.6 0 30 97 24 621.6 0 24 98 28 621.6 0 28 99 24 621.6 0 24 100 37 621.6 0 37 101 23 621.6 0 23 102 33 621.6 0 33 103 42 621.6 0 42 104 38 621.6 0 38 105 40 621.6 0 40 106 28 621.6 0 28 107 25 621.6 0 25 108 27 621.6 0 27 109 35 621.6 0 35 110 28 621.6 0 28 111 30 621.6 0 30 112 40 621.6 0 40 113 41 621.6 0 41 114 28 621.6 0 28 115 33 621.6 0 33 116 28 621.6 0 28 117 36 621.6 0 36 118 26 621.6 0 26 119 34 621.6 0 34 120 29 621.6 0 29 121 36 621.6 0 36 122 42 621.6 0 42 123 141 621.6 0 141 124 27 621.6 0 27 125 46 621.6 0 46 126 45 621.6 0 45 127 49 621.6 0 49 128 43 621.6 0 43 129 53 621.6 0 53 130 32 621.6 0 32 131 38 621.6 0 38 132 26 621.6 0 26 133 28 621.6 0 28 134 48 621.6 0 48 135 36 621.6 0 36 136 68 621.6 0 68 137 53 621.6 0 53 138 37 621.6 0 37 139 74 621.6 0 74 140 55 621.6 0 55 141 36 621.6 0 36 142 48 621.6 0 48 143 50 621.6 0 50 144 41 621.6 0 41 145 74 621.6 0 74 146 64 621.6 0 64 147 47 621.6 0 47 148 54 621.6 0 54 149 64 621.6 0 64 150 52 621.6 0 52 151 64 621.6 0 64 152 69 621.6 0 69 153 57 621.6 0 57 154 62 621.6 0 62 155 59 621.6 0 59 156 49 621.6 0 49 157 56 621.6 0 56 158 62 621.6 0 62 159 50 621.6 0 50 160 82 621.6 0 82 161 62 621.6 0 62 162 74 621.6 0 74 163 85 621.6 0 85 164 84 621.6 0 84 165 99 621.6 0 99 166 119 621.6 0 119 167 541 621.6 0 541 168 431 621.6 0 431 169 519 621.6 0 519 170 98 621.6 0 98 171 231 621.6 0 231 172 140 621.6 0 140 173 114 621.6 0 114 174 88 621.6 0 88 175 93 621.6 0 93 176 86 621.6 0 86 177 86 621.6 0 86 178 87 621.6 0 87 179 87 621.6 0 87 180 113 621.6 0 113 181 94 621.6 0 94 182 113 621.6 0 113 183 136 621.6 0 136 184 161 621.6 0 161 185 107 621.6 0 107 186 81 621.6 0 81 187 94 621.6 0 94 188 125 621.6 0 125 189 132 621.6 0 132 190 121 621.6 0 121 191 153 621.6 0 153 192 109 621.6 0 109 193 107 621.6 0 107 194 112 621.6 0 112 195 127 621.6 0 127 196 104 621.6 0 104 197 136 621.6 0 136 198 123 621.6 0 123 199 156 621.6 0 156 200 426 621.6 0 426 201 710 621.6 0 710 202 666 621.6 0 666 203 307 621.6 0 307 204 106 621.6 0 106 205 141 621.6 0 141 206 121 621.6 0 121 207 109 621.6 0 109 208 145 621.6 0 145 209 124 621.6 0 124 210 147 621.6 0 147 211 249 621.6 0 249 212 133 621.6 0 133 213 138 621.6 0 138 214 163 621.6 0 163 215 152 621.6 0 152 216 162 621.6 0 162 217 141 621.6 0 141 218 143 621.6 0 143 219 147 621.6 0 147 220 156 621.6 0 156 221 227 621.6 0 227 222 163 621.6 0 163 223 275 621.6 0 275 224 467 621.6 0 467 225 381 621.6 0 381 226 586 621.6 0 586 227 1386 621.6 0 1386 228 8080 621.6 0 8080 229 2751 621.6 0 2751 230 24857 621.6 0 24857 231 296 621.6 0 296 232 143 621.6 0 143 233 123 621.6 0 123 234 144 621.6 0 144 235 103 621.6 0 103 236 154 621.6 0 154 237 135 621.6 0 135 238 118 621.6 0 118 239 144 621.6 0 144 240 130 621.6 0 130 241 141 621.6 0 141 242 135 621.6 0 135 243 129 621.6 0 129 244 141 621.6 0 141 245 180 621.6 0 180 246 170 621.6 0 170 247 166 621.6 0 166 248 173 621.6 0 173 249 176 621.6 0 176 250 153 621.6 0 153 251 138 621.6 0 138 252 155 621.6 0 155 253 136 621.6 0 136 254 171 621.6 0 171 255 173 621.6 0 173 256 291 621.6 0 291 257 215 621.6 0 215 258 240 621.6 0 240 259 186 621.6 0 186 260 164 621.6 0 164 261 478 621.6 0 478 262 518 621.6 0 518 263 765 621.6 0 765 264 298 621.6 0 298 265 342 621.6 0 342 266 274 621.6 0 274 267 176 621.6 0 176 268 566 621.6 0 566 269 537 621.6 0 537 270 490 621.6 0 490 271 169 621.6 0 169 272 147 621.6 0 147 273 163 621.6 0 163 274 144 621.6 0 144 275 146 621.6 0 146 276 141 621.6 0 141 277 141 621.6 0 141 278 155 621.6 0 155 279 152 621.6 0 152 280 172 621.6 0 172 281 172 621.6 0 172 282 236 621.6 0 236 283 178 621.6 0 178 284 200 621.6 0 200 285 152 621.6 0 152 286 154 621.6 0 154 287 171 621.6 0 171 288 192 621.6 0 192 289 161 621.6 0 161 290 137 621.6 0 137 291 132 621.6 0 132 292 177 621.6 0 177 293 180 621.6 0 180 294 197 621.6 0 197 295 146 621.6 0 146 296 162 621.6 0 162 297 155 621.6 0 155 298 136 621.6 0 136 299 148 621.6 0 148 300 192 621.6 0 192 301 257 621.6 0 257 Finished in 13.83 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 319,407 Reads with adapters: 140,953 (44.1%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 140,933 (44.1%) Total basepairs processed: 91,892,326 bp Total written (filtered): 39,036,587 bp (42.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 140953 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 29 0.0 3 0 0 29 16 954 0.0 3 0 929 24 1 17 139579 0.0 3 136774 2744 56 5 18 273 0.0 3 34 234 4 1 19 40 0.0 3 0 0 37 3 20 28 0.0 3 0 0 0 28 21 1 0.0 3 1 23 1 0.0 3 1 24 1 0.0 3 1 25 2 0.0 3 1 0 0 1 26 1 0.0 3 1 27 2 0.0 3 1 0 0 1 31 2 0.0 3 0 0 0 2 33 1 0.0 3 1 44 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 1 101 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 153 1 0.0 3 1 158 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 6 0.0 3 0 0 0 6 175 1 0.0 3 1 190 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.97 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 140,933 Reads with adapters: 218 (0.2%) Reads written (passing filters): 140,933 (100.0%) Total basepairs processed: 39,036,587 bp Total written (filtered): 39,035,733 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 218 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 23.9% C: 38.5% G: 33.9% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 184 2202.1 0 184 4 10 550.5 0 10 5 2 137.6 0 2 6 2 34.4 0 2 12 20 0.0 1 0 20