This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.42 s (27 us/read; 2.20 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 1,195,267 (46.9%) Reads that were too short: 38,371 (1.5%) Reads written (passing filters): 1,156,896 (45.4%) Total basepairs processed: 766,381,518 bp Total written (filtered): 312,829,130 bp (40.8%) === Adapter 1 === Sequence: AATCCT; Type: regular 5'; Length: 6; Trimmed: 1195267 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 343 621.6 0 343 7 14174 621.6 0 14174 8 677 621.6 0 677 9 11107 621.6 0 11107 10 518 621.6 0 518 11 752 621.6 0 752 12 11608 621.6 0 11608 13 3532 621.6 0 3532 14 504 621.6 0 504 15 1045 621.6 0 1045 16 578 621.6 0 578 17 816 621.6 0 816 18 2943 621.6 0 2943 19 1444 621.6 0 1444 20 1423 621.6 0 1423 21 414 621.6 0 414 22 591 621.6 0 591 23 1391 621.6 0 1391 24 1771 621.6 0 1771 25 3307 621.6 0 3307 26 5691 621.6 0 5691 27 5801 621.6 0 5801 28 15064 621.6 0 15064 29 34266 621.6 0 34266 30 399086 621.6 0 399086 31 245647 621.6 0 245647 32 381521 621.6 0 381521 33 1395 621.6 0 1395 34 162 621.6 0 162 35 181 621.6 0 181 36 135 621.6 0 135 37 211 621.6 0 211 38 27 621.6 0 27 39 27 621.6 0 27 40 27 621.6 0 27 41 29 621.6 0 29 42 25 621.6 0 25 43 35 621.6 0 35 44 44 621.6 0 44 45 56 621.6 0 56 46 34 621.6 0 34 47 39 621.6 0 39 48 25 621.6 0 25 49 24 621.6 0 24 50 31 621.6 0 31 51 29 621.6 0 29 52 26 621.6 0 26 53 32 621.6 0 32 54 153 621.6 0 153 55 106 621.6 0 106 56 157 621.6 0 157 57 20 621.6 0 20 58 28 621.6 0 28 59 23 621.6 0 23 60 23 621.6 0 23 61 37 621.6 0 37 62 33 621.6 0 33 63 18 621.6 0 18 64 23 621.6 0 23 65 43 621.6 0 43 66 24 621.6 0 24 67 28 621.6 0 28 68 33 621.6 0 33 69 27 621.6 0 27 70 30 621.6 0 30 71 42 621.6 0 42 72 26 621.6 0 26 73 25 621.6 0 25 74 31 621.6 0 31 75 27 621.6 0 27 76 29 621.6 0 29 77 28 621.6 0 28 78 25 621.6 0 25 79 35 621.6 0 35 80 37 621.6 0 37 81 28 621.6 0 28 82 57 621.6 0 57 83 67 621.6 0 67 84 46 621.6 0 46 85 55 621.6 0 55 86 63 621.6 0 63 87 102 621.6 0 102 88 56 621.6 0 56 89 92 621.6 0 92 90 41 621.6 0 41 91 45 621.6 0 45 92 43 621.6 0 43 93 33 621.6 0 33 94 47 621.6 0 47 95 42 621.6 0 42 96 29 621.6 0 29 97 36 621.6 0 36 98 34 621.6 0 34 99 34 621.6 0 34 100 42 621.6 0 42 101 41 621.6 0 41 102 115 621.6 0 115 103 121 621.6 0 121 104 300 621.6 0 300 105 253 621.6 0 253 106 148 621.6 0 148 107 37 621.6 0 37 108 36 621.6 0 36 109 32 621.6 0 32 110 31 621.6 0 31 111 49 621.6 0 49 112 37 621.6 0 37 113 74 621.6 0 74 114 85 621.6 0 85 115 46 621.6 0 46 116 41 621.6 0 41 117 77 621.6 0 77 118 48 621.6 0 48 119 48 621.6 0 48 120 50 621.6 0 50 121 46 621.6 0 46 122 42 621.6 0 42 123 44 621.6 0 44 124 229 621.6 0 229 125 97 621.6 0 97 126 176 621.6 0 176 127 53 621.6 0 53 128 46 621.6 0 46 129 47 621.6 0 47 130 33 621.6 0 33 131 35 621.6 0 35 132 34 621.6 0 34 133 50 621.6 0 50 134 36 621.6 0 36 135 37 621.6 0 37 136 46 621.6 0 46 137 133 621.6 0 133 138 75 621.6 0 75 139 144 621.6 0 144 140 39 621.6 0 39 141 71 621.6 0 71 142 67 621.6 0 67 143 120 621.6 0 120 144 44 621.6 0 44 145 22 621.6 0 22 146 56 621.6 0 56 147 39 621.6 0 39 148 39 621.6 0 39 149 84 621.6 0 84 150 50 621.6 0 50 151 72 621.6 0 72 152 33 621.6 0 33 153 27 621.6 0 27 154 42 621.6 0 42 155 39 621.6 0 39 156 45 621.6 0 45 157 53 621.6 0 53 158 37 621.6 0 37 159 92 621.6 0 92 160 98 621.6 0 98 161 189 621.6 0 189 162 34 621.6 0 34 163 48 621.6 0 48 164 33 621.6 0 33 165 32 621.6 0 32 166 42 621.6 0 42 167 39 621.6 0 39 168 65 621.6 0 65 169 55 621.6 0 55 170 45 621.6 0 45 171 38 621.6 0 38 172 37 621.6 0 37 173 38 621.6 0 38 174 31 621.6 0 31 175 45 621.6 0 45 176 38 621.6 0 38 177 61 621.6 0 61 178 38 621.6 0 38 179 34 621.6 0 34 180 32 621.6 0 32 181 51 621.6 0 51 182 57 621.6 0 57 183 64 621.6 0 64 184 41 621.6 0 41 185 41 621.6 0 41 186 42 621.6 0 42 187 29 621.6 0 29 188 46 621.6 0 46 189 53 621.6 0 53 190 52 621.6 0 52 191 44 621.6 0 44 192 50 621.6 0 50 193 45 621.6 0 45 194 43 621.6 0 43 195 40 621.6 0 40 196 35 621.6 0 35 197 36 621.6 0 36 198 49 621.6 0 49 199 52 621.6 0 52 200 46 621.6 0 46 201 54 621.6 0 54 202 57 621.6 0 57 203 35 621.6 0 35 204 49 621.6 0 49 205 106 621.6 0 106 206 120 621.6 0 120 207 115 621.6 0 115 208 42 621.6 0 42 209 32 621.6 0 32 210 48 621.6 0 48 211 45 621.6 0 45 212 53 621.6 0 53 213 53 621.6 0 53 214 52 621.6 0 52 215 43 621.6 0 43 216 52 621.6 0 52 217 52 621.6 0 52 218 43 621.6 0 43 219 51 621.6 0 51 220 74 621.6 0 74 221 48 621.6 0 48 222 47 621.6 0 47 223 53 621.6 0 53 224 33 621.6 0 33 225 49 621.6 0 49 226 96 621.6 0 96 227 61 621.6 0 61 228 109 621.6 0 109 229 55 621.6 0 55 230 52 621.6 0 52 231 47 621.6 0 47 232 72 621.6 0 72 233 74 621.6 0 74 234 32 621.6 0 32 235 43 621.6 0 43 236 60 621.6 0 60 237 45 621.6 0 45 238 62 621.6 0 62 239 68 621.6 0 68 240 58 621.6 0 58 241 94 621.6 0 94 242 73 621.6 0 73 243 82 621.6 0 82 244 68 621.6 0 68 245 90 621.6 0 90 246 146 621.6 0 146 247 204 621.6 0 204 248 99 621.6 0 99 249 111 621.6 0 111 250 174 621.6 0 174 251 84 621.6 0 84 252 120 621.6 0 120 253 190 621.6 0 190 254 1455 621.6 0 1455 255 1664 621.6 0 1664 256 2897 621.6 0 2897 257 424 621.6 0 424 258 394 621.6 0 394 259 335 621.6 0 335 260 5440 621.6 0 5440 261 5248 621.6 0 5248 262 9592 621.6 0 9592 263 113 621.6 0 113 264 54 621.6 0 54 265 85 621.6 0 85 266 59 621.6 0 59 267 81 621.6 0 81 268 76 621.6 0 76 269 84 621.6 0 84 270 85 621.6 0 85 271 73 621.6 0 73 272 167 621.6 0 167 273 102 621.6 0 102 274 156 621.6 0 156 275 312 621.6 0 312 276 644 621.6 0 644 277 896 621.6 0 896 278 1037 621.6 0 1037 279 262 621.6 0 262 280 614 621.6 0 614 281 546 621.6 0 546 282 643 621.6 0 643 283 169 621.6 0 169 284 51 621.6 0 51 285 36 621.6 0 36 286 46 621.6 0 46 287 50 621.6 0 50 288 51 621.6 0 51 289 66 621.6 0 66 290 35 621.6 0 35 291 53 621.6 0 53 292 47 621.6 0 47 293 61 621.6 0 61 294 69 621.6 0 69 295 50 621.6 0 50 296 47 621.6 0 47 297 21 621.6 0 21 298 31 621.6 0 31 299 37 621.6 0 37 300 37 621.6 0 37 301 55 621.6 0 55 Finished in 47.12 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 1,156,896 Reads with adapters: 11,915 (1.0%) Reads that were too short: 135 (0.0%) Reads written (passing filters): 11,780 (1.0%) Total basepairs processed: 312,829,130 bp Total written (filtered): 3,219,837 bp (1.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11915 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 6 0.0 3 0 0 6 16 87 0.0 3 0 86 1 17 11499 0.0 3 11288 206 5 18 34 0.0 3 2 31 0 1 19 13 0.0 3 3 0 8 2 20 3 0.0 3 0 0 0 3 24 1 0.0 3 0 0 0 1 25 2 0.0 3 0 0 0 2 31 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 55 1 0.0 3 1 61 3 0.0 3 0 0 0 3 62 2 0.0 3 0 1 0 1 67 2 0.0 3 0 0 0 2 68 3 0.0 3 0 0 0 3 69 2 0.0 3 0 0 1 1 70 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 1 109 1 0.0 3 1 112 1 0.0 3 1 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 3 0.0 3 0 0 0 3 130 2 0.0 3 1 0 1 131 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 141 11 0.0 3 0 0 0 11 142 2 0.0 3 0 0 0 2 146 1 0.0 3 1 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 152 1 0.0 3 1 154 1 0.0 3 0 0 0 1 156 4 0.0 3 0 0 1 3 159 1 0.0 3 0 0 0 1 161 10 0.0 3 0 0 0 10 162 3 0.0 3 0 0 0 3 164 4 0.0 3 0 0 0 4 167 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 169 11 0.0 3 0 0 0 11 171 4 0.0 3 0 0 0 4 177 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 16 0.0 3 0 0 0 16 182 33 0.0 3 0 0 0 33 183 3 0.0 3 0 0 0 3 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 187 2 0.0 3 0 1 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 195 3 0.0 3 0 0 1 2 198 4 0.0 3 0 0 0 4 199 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 1 210 1 0.0 3 1 214 1 0.0 3 0 0 0 1 221 1 0.0 3 1 225 1 0.0 3 0 0 0 1 232 1 0.0 3 0 1 246 1 0.0 3 0 0 0 1 247 3 0.0 3 0 0 0 3 248 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 256 15 0.0 3 0 0 1 14 257 7 0.0 3 0 0 0 7 258 29 0.0 3 0 0 1 28 259 25 0.0 3 0 0 0 25 260 2 0.0 3 0 0 0 2 264 3 0.0 3 0 0 0 3 265 1 0.0 3 0 0 0 1 266 2 0.0 3 0 0 0 2 268 1 0.0 3 1 269 1 0.0 3 0 0 0 1 275 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.41 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 11,780 Reads with adapters: 15 (0.1%) Reads written (passing filters): 11,780 (100.0%) Total basepairs processed: 3,219,837 bp Total written (filtered): 3,219,785 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 15 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.3% C: 20.0% G: 33.3% T: 13.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 9 184.1 0 9 4 5 46.0 0 5 5 1 11.5 0 1