This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.57 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 262,563 (10.3%) Reads that were too short: 28,768 (1.1%) Reads written (passing filters): 233,795 (9.2%) Total basepairs processed: 766,381,518 bp Total written (filtered): 63,144,302 bp (8.2%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 262563 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1251 621.6 0 1251 7 2086 621.6 0 2086 8 89643 621.6 0 89643 9 82728 621.6 0 82728 10 143 621.6 0 143 11 49 621.6 0 49 12 58 621.6 0 58 13 54 621.6 0 54 14 41 621.6 0 41 15 60 621.6 0 60 16 49 621.6 0 49 17 55 621.6 0 55 18 78 621.6 0 78 19 44 621.6 0 44 20 49 621.6 0 49 21 41 621.6 0 41 22 47 621.6 0 47 23 67 621.6 0 67 24 58 621.6 0 58 25 73 621.6 0 73 26 64 621.6 0 64 27 46 621.6 0 46 28 46 621.6 0 46 29 51 621.6 0 51 30 50 621.6 0 50 31 52 621.6 0 52 32 54 621.6 0 54 33 60 621.6 0 60 34 35 621.6 0 35 35 68 621.6 0 68 36 51 621.6 0 51 37 52 621.6 0 52 38 40 621.6 0 40 39 78 621.6 0 78 40 36 621.6 0 36 41 64 621.6 0 64 42 102 621.6 0 102 43 58 621.6 0 58 44 51 621.6 0 51 45 62 621.6 0 62 46 46 621.6 0 46 47 58 621.6 0 58 48 41 621.6 0 41 49 56 621.6 0 56 50 54 621.6 0 54 51 64 621.6 0 64 52 110 621.6 0 110 53 85 621.6 0 85 54 697 621.6 0 697 55 107 621.6 0 107 56 55 621.6 0 55 57 95 621.6 0 95 58 63 621.6 0 63 59 69 621.6 0 69 60 54 621.6 0 54 61 80 621.6 0 80 62 86 621.6 0 86 63 126 621.6 0 126 64 129 621.6 0 129 65 193 621.6 0 193 66 248 621.6 0 248 67 611 621.6 0 611 68 1461 621.6 0 1461 69 18640 621.6 0 18640 70 3646 621.6 0 3646 71 5369 621.6 0 5369 72 65 621.6 0 65 73 45 621.6 0 45 74 54 621.6 0 54 75 62 621.6 0 62 76 51 621.6 0 51 77 117 621.6 0 117 78 63 621.6 0 63 79 74 621.6 0 74 80 234 621.6 0 234 81 112 621.6 0 112 82 154 621.6 0 154 83 67 621.6 0 67 84 43 621.6 0 43 85 47 621.6 0 47 86 75 621.6 0 75 87 109 621.6 0 109 88 93 621.6 0 93 89 85 621.6 0 85 90 108 621.6 0 108 91 84 621.6 0 84 92 73 621.6 0 73 93 80 621.6 0 80 94 67 621.6 0 67 95 63 621.6 0 63 96 184 621.6 0 184 97 126 621.6 0 126 98 96 621.6 0 96 99 99 621.6 0 99 100 556 621.6 0 556 101 837 621.6 0 837 102 1632 621.6 0 1632 103 99 621.6 0 99 104 61 621.6 0 61 105 70 621.6 0 70 106 61 621.6 0 61 107 59 621.6 0 59 108 60 621.6 0 60 109 49 621.6 0 49 110 78 621.6 0 78 111 73 621.6 0 73 112 75 621.6 0 75 113 103 621.6 0 103 114 84 621.6 0 84 115 86 621.6 0 86 116 86 621.6 0 86 117 81 621.6 0 81 118 88 621.6 0 88 119 97 621.6 0 97 120 105 621.6 0 105 121 72 621.6 0 72 122 104 621.6 0 104 123 111 621.6 0 111 124 101 621.6 0 101 125 89 621.6 0 89 126 79 621.6 0 79 127 92 621.6 0 92 128 64 621.6 0 64 129 71 621.6 0 71 130 111 621.6 0 111 131 123 621.6 0 123 132 395 621.6 0 395 133 242 621.6 0 242 134 494 621.6 0 494 135 170 621.6 0 170 136 263 621.6 0 263 137 353 621.6 0 353 138 406 621.6 0 406 139 115 621.6 0 115 140 99 621.6 0 99 141 66 621.6 0 66 142 73 621.6 0 73 143 93 621.6 0 93 144 104 621.6 0 104 145 76 621.6 0 76 146 101 621.6 0 101 147 102 621.6 0 102 148 107 621.6 0 107 149 82 621.6 0 82 150 95 621.6 0 95 151 112 621.6 0 112 152 91 621.6 0 91 153 102 621.6 0 102 154 133 621.6 0 133 155 73 621.6 0 73 156 139 621.6 0 139 157 207 621.6 0 207 158 215 621.6 0 215 159 469 621.6 0 469 160 147 621.6 0 147 161 130 621.6 0 130 162 112 621.6 0 112 163 125 621.6 0 125 164 113 621.6 0 113 165 108 621.6 0 108 166 87 621.6 0 87 167 236 621.6 0 236 168 192 621.6 0 192 169 158 621.6 0 158 170 158 621.6 0 158 171 127 621.6 0 127 172 150 621.6 0 150 173 106 621.6 0 106 174 117 621.6 0 117 175 113 621.6 0 113 176 302 621.6 0 302 177 693 621.6 0 693 178 708 621.6 0 708 179 922 621.6 0 922 180 347 621.6 0 347 181 353 621.6 0 353 182 918 621.6 0 918 183 421 621.6 0 421 184 1090 621.6 0 1090 185 395 621.6 0 395 186 139 621.6 0 139 187 183 621.6 0 183 188 149 621.6 0 149 189 215 621.6 0 215 190 176 621.6 0 176 191 134 621.6 0 134 192 129 621.6 0 129 193 107 621.6 0 107 194 116 621.6 0 116 195 106 621.6 0 106 196 101 621.6 0 101 197 200 621.6 0 200 198 183 621.6 0 183 199 185 621.6 0 185 200 188 621.6 0 188 201 155 621.6 0 155 202 1110 621.6 0 1110 203 953 621.6 0 953 204 1194 621.6 0 1194 205 516 621.6 0 516 206 110 621.6 0 110 207 80 621.6 0 80 208 94 621.6 0 94 209 127 621.6 0 127 210 131 621.6 0 131 211 209 621.6 0 209 212 182 621.6 0 182 213 157 621.6 0 157 214 127 621.6 0 127 215 120 621.6 0 120 216 123 621.6 0 123 217 151 621.6 0 151 218 157 621.6 0 157 219 152 621.6 0 152 220 138 621.6 0 138 221 144 621.6 0 144 222 393 621.6 0 393 223 496 621.6 0 496 224 600 621.6 0 600 225 363 621.6 0 363 226 184 621.6 0 184 227 502 621.6 0 502 228 230 621.6 0 230 229 340 621.6 0 340 230 100 621.6 0 100 231 99 621.6 0 99 232 102 621.6 0 102 233 94 621.6 0 94 234 94 621.6 0 94 235 104 621.6 0 104 236 76 621.6 0 76 237 107 621.6 0 107 238 137 621.6 0 137 239 102 621.6 0 102 240 136 621.6 0 136 241 128 621.6 0 128 242 476 621.6 0 476 243 502 621.6 0 502 244 492 621.6 0 492 245 228 621.6 0 228 246 116 621.6 0 116 247 107 621.6 0 107 248 78 621.6 0 78 249 81 621.6 0 81 250 112 621.6 0 112 251 192 621.6 0 192 252 181 621.6 0 181 253 271 621.6 0 271 254 130 621.6 0 130 255 318 621.6 0 318 256 294 621.6 0 294 257 325 621.6 0 325 258 234 621.6 0 234 259 157 621.6 0 157 260 137 621.6 0 137 261 140 621.6 0 140 262 165 621.6 0 165 263 176 621.6 0 176 264 244 621.6 0 244 265 449 621.6 0 449 266 331 621.6 0 331 267 352 621.6 0 352 268 271 621.6 0 271 269 2241 621.6 0 2241 270 677 621.6 0 677 271 1997 621.6 0 1997 272 285 621.6 0 285 273 419 621.6 0 419 274 201 621.6 0 201 275 158 621.6 0 158 276 330 621.6 0 330 277 411 621.6 0 411 278 398 621.6 0 398 279 302 621.6 0 302 280 123 621.6 0 123 281 111 621.6 0 111 282 97 621.6 0 97 283 162 621.6 0 162 284 182 621.6 0 182 285 210 621.6 0 210 286 195 621.6 0 195 287 133 621.6 0 133 288 113 621.6 0 113 289 130 621.6 0 130 290 153 621.6 0 153 291 614 621.6 0 614 292 465 621.6 0 465 293 429 621.6 0 429 294 286 621.6 0 286 295 93 621.6 0 93 296 89 621.6 0 89 297 110 621.6 0 110 298 132 621.6 0 132 299 217 621.6 0 217 300 212 621.6 0 212 301 172 621.6 0 172 Finished in 11.45 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 233,795 Reads with adapters: 86,621 (37.0%) Reads that were too short: 88 (0.0%) Reads written (passing filters): 86,533 (37.0%) Total basepairs processed: 63,144,302 bp Total written (filtered): 23,487,850 bp (37.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 86621 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 9 0.0 3 0 0 0 6 3 18 59 0.0 3 0 0 34 16 9 19 1491 0.0 3 0 1302 88 73 28 20 83933 0.0 4 77936 4630 993 261 113 21 182 0.0 4 27 142 6 3 4 22 22 0.0 4 2 2 9 1 8 23 319 0.0 4 1 1 1 229 87 24 5 0.0 4 1 0 0 0 4 29 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 34 3 0.0 4 1 0 0 0 2 35 1 0.0 4 0 1 36 1 0.0 4 0 0 0 0 1 46 2 0.0 4 2 54 1 0.0 4 0 0 0 0 1 60 2 0.0 4 0 0 0 0 2 61 1 0.0 4 0 0 0 0 1 75 2 0.0 4 0 0 0 0 2 78 1 0.0 4 0 0 0 0 1 87 2 0.0 4 0 0 0 0 2 88 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 6 0.0 4 0 0 0 0 6 104 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 0 2 109 1 0.0 4 0 0 0 0 1 110 1 0.0 4 1 111 2 0.0 4 0 0 0 0 2 121 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 5 0.0 4 0 0 0 0 5 127 466 0.0 4 0 0 0 0 466 128 2 0.0 4 0 0 0 0 2 129 3 0.0 4 0 0 0 0 3 130 3 0.0 4 0 0 0 0 3 132 1 0.0 4 0 0 0 0 1 134 3 0.0 4 0 0 0 0 3 135 6 0.0 4 0 0 0 0 6 136 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 144 2 0.0 4 0 0 1 0 1 150 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 168 1 0.0 4 0 0 0 0 1 184 2 0.0 4 0 0 0 0 2 189 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 0 2 208 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 3 0.0 4 0 0 0 0 3 220 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 4 0.0 4 0 0 0 0 4 232 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 0 2 234 6 0.0 4 0 0 0 0 6 235 2 0.0 4 0 0 0 0 2 238 2 0.0 4 0 0 0 0 2 245 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 273 2 0.0 4 0 0 0 0 2 281 1 0.0 4 0 0 0 0 1 282 11 0.0 4 0 0 0 0 11 283 3 0.0 4 0 0 0 0 3 284 1 0.0 4 0 0 0 0 1 285 2 0.0 4 0 0 0 0 2 288 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.23 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 86,533 Reads with adapters: 24,132 (27.9%) Reads written (passing filters): 86,533 (100.0%) Total basepairs processed: 23,487,850 bp Total written (filtered): 23,242,265 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 24132 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.9% C: 7.8% G: 88.0% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 44 1352.1 0 44 4 19 338.0 0 19 5 2 84.5 0 2 6 5 21.1 0 5 7 7 5.3 0 7 8 6 1.3 0 6 9 8609 0.3 0 631 7978 10 14101 0.1 1 1034 13067 11 1146 0.0 1 33 1113 12 69 0.0 1 1 68 13 52 0.0 1 1 51 39 1 0.0 1 1 40 1 0.0 1 0 1 58 1 0.0 1 1 60 1 0.0 1 1 109 1 0.0 1 1 150 32 0.0 1 21 11 151 2 0.0 1 2 153 1 0.0 1 1 155 1 0.0 1 1 169 2 0.0 1 2 172 1 0.0 1 0 1 189 1 0.0 1 0 1 191 1 0.0 1 0 1 192 1 0.0 1 0 1 208 9 0.0 1 8 1 209 1 0.0 1 1 223 1 0.0 1 1 225 1 0.0 1 1 226 1 0.0 1 1 257 11 0.0 1 10 1 259 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.