This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.92 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 254,075 (10.0%) Reads that were too short: 15,790 (0.6%) Reads written (passing filters): 238,285 (9.4%) Total basepairs processed: 766,381,518 bp Total written (filtered): 65,414,197 bp (8.5%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 254075 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1073 621.6 0 1073 7 1649 621.6 0 1649 8 88172 621.6 0 88172 9 93176 621.6 0 93176 10 162 621.6 0 162 11 64 621.6 0 64 12 68 621.6 0 68 13 61 621.6 0 61 14 62 621.6 0 62 15 60 621.6 0 60 16 49 621.6 0 49 17 48 621.6 0 48 18 91 621.6 0 91 19 89 621.6 0 89 20 52 621.6 0 52 21 42 621.6 0 42 22 65 621.6 0 65 23 50 621.6 0 50 24 54 621.6 0 54 25 63 621.6 0 63 26 46 621.6 0 46 27 54 621.6 0 54 28 49 621.6 0 49 29 54 621.6 0 54 30 60 621.6 0 60 31 67 621.6 0 67 32 78 621.6 0 78 33 459 621.6 0 459 34 89 621.6 0 89 35 82 621.6 0 82 36 84 621.6 0 84 37 47 621.6 0 47 38 51 621.6 0 51 39 67 621.6 0 67 40 53 621.6 0 53 41 57 621.6 0 57 42 120 621.6 0 120 43 102 621.6 0 102 44 69 621.6 0 69 45 70 621.6 0 70 46 78 621.6 0 78 47 55 621.6 0 55 48 62 621.6 0 62 49 55 621.6 0 55 50 57 621.6 0 57 51 82 621.6 0 82 52 126 621.6 0 126 53 78 621.6 0 78 54 85 621.6 0 85 55 60 621.6 0 60 56 69 621.6 0 69 57 140 621.6 0 140 58 92 621.6 0 92 59 84 621.6 0 84 60 66 621.6 0 66 61 93 621.6 0 93 62 82 621.6 0 82 63 104 621.6 0 104 64 132 621.6 0 132 65 212 621.6 0 212 66 237 621.6 0 237 67 623 621.6 0 623 68 1525 621.6 0 1525 69 21673 621.6 0 21673 70 4176 621.6 0 4176 71 6219 621.6 0 6219 72 75 621.6 0 75 73 53 621.6 0 53 74 56 621.6 0 56 75 59 621.6 0 59 76 60 621.6 0 60 77 58 621.6 0 58 78 68 621.6 0 68 79 59 621.6 0 59 80 122 621.6 0 122 81 78 621.6 0 78 82 109 621.6 0 109 83 84 621.6 0 84 84 68 621.6 0 68 85 65 621.6 0 65 86 79 621.6 0 79 87 97 621.6 0 97 88 103 621.6 0 103 89 100 621.6 0 100 90 83 621.6 0 83 91 83 621.6 0 83 92 83 621.6 0 83 93 90 621.6 0 90 94 102 621.6 0 102 95 94 621.6 0 94 96 67 621.6 0 67 97 95 621.6 0 95 98 85 621.6 0 85 99 69 621.6 0 69 100 179 621.6 0 179 101 248 621.6 0 248 102 358 621.6 0 358 103 77 621.6 0 77 104 66 621.6 0 66 105 74 621.6 0 74 106 72 621.6 0 72 107 79 621.6 0 79 108 75 621.6 0 75 109 86 621.6 0 86 110 80 621.6 0 80 111 86 621.6 0 86 112 61 621.6 0 61 113 81 621.6 0 81 114 77 621.6 0 77 115 80 621.6 0 80 116 92 621.6 0 92 117 86 621.6 0 86 118 96 621.6 0 96 119 115 621.6 0 115 120 97 621.6 0 97 121 70 621.6 0 70 122 71 621.6 0 71 123 62 621.6 0 62 124 89 621.6 0 89 125 76 621.6 0 76 126 74 621.6 0 74 127 86 621.6 0 86 128 67 621.6 0 67 129 73 621.6 0 73 130 104 621.6 0 104 131 98 621.6 0 98 132 233 621.6 0 233 133 222 621.6 0 222 134 294 621.6 0 294 135 144 621.6 0 144 136 182 621.6 0 182 137 195 621.6 0 195 138 178 621.6 0 178 139 110 621.6 0 110 140 75 621.6 0 75 141 55 621.6 0 55 142 76 621.6 0 76 143 70 621.6 0 70 144 78 621.6 0 78 145 92 621.6 0 92 146 71 621.6 0 71 147 92 621.6 0 92 148 89 621.6 0 89 149 69 621.6 0 69 150 100 621.6 0 100 151 105 621.6 0 105 152 95 621.6 0 95 153 87 621.6 0 87 154 108 621.6 0 108 155 68 621.6 0 68 156 92 621.6 0 92 157 115 621.6 0 115 158 116 621.6 0 116 159 399 621.6 0 399 160 120 621.6 0 120 161 121 621.6 0 121 162 86 621.6 0 86 163 119 621.6 0 119 164 82 621.6 0 82 165 67 621.6 0 67 166 102 621.6 0 102 167 197 621.6 0 197 168 134 621.6 0 134 169 112 621.6 0 112 170 99 621.6 0 99 171 104 621.6 0 104 172 88 621.6 0 88 173 103 621.6 0 103 174 77 621.6 0 77 175 100 621.6 0 100 176 169 621.6 0 169 177 294 621.6 0 294 178 326 621.6 0 326 179 422 621.6 0 422 180 214 621.6 0 214 181 273 621.6 0 273 182 242 621.6 0 242 183 174 621.6 0 174 184 334 621.6 0 334 185 340 621.6 0 340 186 102 621.6 0 102 187 113 621.6 0 113 188 110 621.6 0 110 189 124 621.6 0 124 190 120 621.6 0 120 191 118 621.6 0 118 192 76 621.6 0 76 193 102 621.6 0 102 194 83 621.6 0 83 195 79 621.6 0 79 196 88 621.6 0 88 197 99 621.6 0 99 198 101 621.6 0 101 199 116 621.6 0 116 200 109 621.6 0 109 201 84 621.6 0 84 202 352 621.6 0 352 203 339 621.6 0 339 204 305 621.6 0 305 205 180 621.6 0 180 206 86 621.6 0 86 207 82 621.6 0 82 208 88 621.6 0 88 209 86 621.6 0 86 210 110 621.6 0 110 211 119 621.6 0 119 212 100 621.6 0 100 213 146 621.6 0 146 214 84 621.6 0 84 215 84 621.6 0 84 216 93 621.6 0 93 217 98 621.6 0 98 218 75 621.6 0 75 219 80 621.6 0 80 220 95 621.6 0 95 221 97 621.6 0 97 222 135 621.6 0 135 223 188 621.6 0 188 224 193 621.6 0 193 225 151 621.6 0 151 226 118 621.6 0 118 227 347 621.6 0 347 228 176 621.6 0 176 229 229 621.6 0 229 230 84 621.6 0 84 231 91 621.6 0 91 232 84 621.6 0 84 233 89 621.6 0 89 234 82 621.6 0 82 235 94 621.6 0 94 236 63 621.6 0 63 237 73 621.6 0 73 238 97 621.6 0 97 239 89 621.6 0 89 240 79 621.6 0 79 241 77 621.6 0 77 242 162 621.6 0 162 243 194 621.6 0 194 244 186 621.6 0 186 245 122 621.6 0 122 246 129 621.6 0 129 247 82 621.6 0 82 248 71 621.6 0 71 249 76 621.6 0 76 250 128 621.6 0 128 251 121 621.6 0 121 252 105 621.6 0 105 253 105 621.6 0 105 254 105 621.6 0 105 255 129 621.6 0 129 256 165 621.6 0 165 257 147 621.6 0 147 258 109 621.6 0 109 259 103 621.6 0 103 260 101 621.6 0 101 261 86 621.6 0 86 262 98 621.6 0 98 263 123 621.6 0 123 264 121 621.6 0 121 265 168 621.6 0 168 266 163 621.6 0 163 267 160 621.6 0 160 268 158 621.6 0 158 269 1634 621.6 0 1634 270 426 621.6 0 426 271 1106 621.6 0 1106 272 164 621.6 0 164 273 160 621.6 0 160 274 138 621.6 0 138 275 119 621.6 0 119 276 172 621.6 0 172 277 220 621.6 0 220 278 168 621.6 0 168 279 130 621.6 0 130 280 87 621.6 0 87 281 115 621.6 0 115 282 85 621.6 0 85 283 109 621.6 0 109 284 129 621.6 0 129 285 117 621.6 0 117 286 116 621.6 0 116 287 113 621.6 0 113 288 117 621.6 0 117 289 87 621.6 0 87 290 107 621.6 0 107 291 205 621.6 0 205 292 179 621.6 0 179 293 165 621.6 0 165 294 123 621.6 0 123 295 85 621.6 0 85 296 102 621.6 0 102 297 98 621.6 0 98 298 111 621.6 0 111 299 170 621.6 0 170 300 150 621.6 0 150 301 128 621.6 0 128 Finished in 10.38 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 238,285 Reads with adapters: 87,154 (36.6%) Reads that were too short: 29 (0.0%) Reads written (passing filters): 87,125 (36.6%) Total basepairs processed: 65,414,197 bp Total written (filtered): 24,042,972 bp (36.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 87154 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 29 0.0 3 0 0 25 4 16 532 0.0 3 0 518 13 1 17 86182 0.0 3 84648 1480 52 2 18 209 0.0 3 19 186 3 1 19 40 0.0 3 0 1 32 7 20 96 0.0 3 80 3 0 13 22 1 0.0 3 1 25 1 0.0 3 0 1 27 1 0.0 3 0 0 0 1 28 1 0.0 3 1 29 1 0.0 3 0 0 0 1 34 4 0.0 3 0 0 0 4 37 1 0.0 3 1 40 1 0.0 3 1 49 1 0.0 3 0 0 0 1 55 4 0.0 3 0 0 0 4 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 7 0.0 3 0 0 0 7 94 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 130 3 0.0 3 0 0 0 3 146 4 0.0 3 0 0 0 4 156 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 171 1 0.0 3 1 183 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 219 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.05 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 87,125 Reads with adapters: 105 (0.1%) Reads written (passing filters): 87,125 (100.0%) Total basepairs processed: 24,042,972 bp Total written (filtered): 24,042,598 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 105 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 41.9% C: 10.5% G: 42.9% T: 4.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 57 1361.3 0 57 4 42 340.3 0 42 5 5 85.1 0 5 10 1 0.1 1 0 1