This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.81 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 105,646 (4.8%) Reads that were too short: 14,434 (0.7%) Reads written (passing filters): 91,212 (4.2%) Total basepairs processed: 659,880,795 bp Total written (filtered): 24,688,272 bp (3.7%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 105646 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1174 535.2 0 1174 7 31283 535.2 0 31283 8 1652 535.2 0 1652 9 35672 535.2 0 35672 10 168 535.2 0 168 11 84 535.2 0 84 12 80 535.2 0 80 13 75 535.2 0 75 14 71 535.2 0 71 15 86 535.2 0 86 16 78 535.2 0 78 17 67 535.2 0 67 18 67 535.2 0 67 19 69 535.2 0 69 20 91 535.2 0 91 21 91 535.2 0 91 22 159 535.2 0 159 23 367 535.2 0 367 24 354 535.2 0 354 25 396 535.2 0 396 26 57 535.2 0 57 27 78 535.2 0 78 28 77 535.2 0 77 29 80 535.2 0 80 30 61 535.2 0 61 31 60 535.2 0 60 32 51 535.2 0 51 33 83 535.2 0 83 34 103 535.2 0 103 35 114 535.2 0 114 36 83 535.2 0 83 37 69 535.2 0 69 38 65 535.2 0 65 39 82 535.2 0 82 40 58 535.2 0 58 41 60 535.2 0 60 42 57 535.2 0 57 43 72 535.2 0 72 44 62 535.2 0 62 45 77 535.2 0 77 46 55 535.2 0 55 47 71 535.2 0 71 48 69 535.2 0 69 49 55 535.2 0 55 50 71 535.2 0 71 51 55 535.2 0 55 52 60 535.2 0 60 53 49 535.2 0 49 54 58 535.2 0 58 55 80 535.2 0 80 56 77 535.2 0 77 57 67 535.2 0 67 58 58 535.2 0 58 59 51 535.2 0 51 60 81 535.2 0 81 61 71 535.2 0 71 62 37 535.2 0 37 63 72 535.2 0 72 64 68 535.2 0 68 65 71 535.2 0 71 66 65 535.2 0 65 67 50 535.2 0 50 68 69 535.2 0 69 69 94 535.2 0 94 70 79 535.2 0 79 71 83 535.2 0 83 72 35 535.2 0 35 73 93 535.2 0 93 74 107 535.2 0 107 75 56 535.2 0 56 76 58 535.2 0 58 77 42 535.2 0 42 78 71 535.2 0 71 79 47 535.2 0 47 80 49 535.2 0 49 81 58 535.2 0 58 82 66 535.2 0 66 83 64 535.2 0 64 84 79 535.2 0 79 85 73 535.2 0 73 86 96 535.2 0 96 87 93 535.2 0 93 88 719 535.2 0 719 89 594 535.2 0 594 90 546 535.2 0 546 91 39 535.2 0 39 92 52 535.2 0 52 93 47 535.2 0 47 94 64 535.2 0 64 95 70 535.2 0 70 96 236 535.2 0 236 97 263 535.2 0 263 98 174 535.2 0 174 99 95 535.2 0 95 100 142 535.2 0 142 101 646 535.2 0 646 102 686 535.2 0 686 103 642 535.2 0 642 104 86 535.2 0 86 105 72 535.2 0 72 106 66 535.2 0 66 107 43 535.2 0 43 108 44 535.2 0 44 109 50 535.2 0 50 110 81 535.2 0 81 111 82 535.2 0 82 112 66 535.2 0 66 113 84 535.2 0 84 114 82 535.2 0 82 115 87 535.2 0 87 116 56 535.2 0 56 117 84 535.2 0 84 118 67 535.2 0 67 119 69 535.2 0 69 120 69 535.2 0 69 121 74 535.2 0 74 122 73 535.2 0 73 123 69 535.2 0 69 124 71 535.2 0 71 125 94 535.2 0 94 126 179 535.2 0 179 127 1036 535.2 0 1036 128 1005 535.2 0 1005 129 877 535.2 0 877 130 84 535.2 0 84 131 158 535.2 0 158 132 100 535.2 0 100 133 86 535.2 0 86 134 69 535.2 0 69 135 102 535.2 0 102 136 73 535.2 0 73 137 51 535.2 0 51 138 66 535.2 0 66 139 53 535.2 0 53 140 70 535.2 0 70 141 86 535.2 0 86 142 56 535.2 0 56 143 57 535.2 0 57 144 46 535.2 0 46 145 81 535.2 0 81 146 71 535.2 0 71 147 86 535.2 0 86 148 138 535.2 0 138 149 95 535.2 0 95 150 53 535.2 0 53 151 61 535.2 0 61 152 53 535.2 0 53 153 64 535.2 0 64 154 50 535.2 0 50 155 50 535.2 0 50 156 67 535.2 0 67 157 53 535.2 0 53 158 60 535.2 0 60 159 54 535.2 0 54 160 53 535.2 0 53 161 66 535.2 0 66 162 57 535.2 0 57 163 53 535.2 0 53 164 42 535.2 0 42 165 54 535.2 0 54 166 55 535.2 0 55 167 73 535.2 0 73 168 78 535.2 0 78 169 86 535.2 0 86 170 63 535.2 0 63 171 56 535.2 0 56 172 51 535.2 0 51 173 41 535.2 0 41 174 34 535.2 0 34 175 37 535.2 0 37 176 88 535.2 0 88 177 73 535.2 0 73 178 67 535.2 0 67 179 38 535.2 0 38 180 39 535.2 0 39 181 52 535.2 0 52 182 114 535.2 0 114 183 96 535.2 0 96 184 63 535.2 0 63 185 54 535.2 0 54 186 55 535.2 0 55 187 52 535.2 0 52 188 47 535.2 0 47 189 75 535.2 0 75 190 54 535.2 0 54 191 34 535.2 0 34 192 49 535.2 0 49 193 80 535.2 0 80 194 80 535.2 0 80 195 232 535.2 0 232 196 154 535.2 0 154 197 98 535.2 0 98 198 78 535.2 0 78 199 71 535.2 0 71 200 121 535.2 0 121 201 139 535.2 0 139 202 174 535.2 0 174 203 145 535.2 0 145 204 86 535.2 0 86 205 89 535.2 0 89 206 75 535.2 0 75 207 92 535.2 0 92 208 113 535.2 0 113 209 164 535.2 0 164 210 123 535.2 0 123 211 60 535.2 0 60 212 59 535.2 0 59 213 69 535.2 0 69 214 107 535.2 0 107 215 100 535.2 0 100 216 84 535.2 0 84 217 73 535.2 0 73 218 57 535.2 0 57 219 55 535.2 0 55 220 47 535.2 0 47 221 75 535.2 0 75 222 76 535.2 0 76 223 94 535.2 0 94 224 84 535.2 0 84 225 65 535.2 0 65 226 66 535.2 0 66 227 67 535.2 0 67 228 46 535.2 0 46 229 76 535.2 0 76 230 82 535.2 0 82 231 95 535.2 0 95 232 66 535.2 0 66 233 89 535.2 0 89 234 117 535.2 0 117 235 172 535.2 0 172 236 192 535.2 0 192 237 98 535.2 0 98 238 80 535.2 0 80 239 155 535.2 0 155 240 207 535.2 0 207 241 234 535.2 0 234 242 168 535.2 0 168 243 101 535.2 0 101 244 51 535.2 0 51 245 69 535.2 0 69 246 58 535.2 0 58 247 63 535.2 0 63 248 84 535.2 0 84 249 79 535.2 0 79 250 86 535.2 0 86 251 74 535.2 0 74 252 67 535.2 0 67 253 91 535.2 0 91 254 211 535.2 0 211 255 186 535.2 0 186 256 216 535.2 0 216 257 136 535.2 0 136 258 99 535.2 0 99 259 667 535.2 0 667 260 1490 535.2 0 1490 261 1143 535.2 0 1143 262 464 535.2 0 464 263 102 535.2 0 102 264 37 535.2 0 37 265 54 535.2 0 54 266 71 535.2 0 71 267 63 535.2 0 63 268 78 535.2 0 78 269 63 535.2 0 63 270 53 535.2 0 53 271 54 535.2 0 54 272 57 535.2 0 57 273 66 535.2 0 66 274 64 535.2 0 64 275 69 535.2 0 69 276 85 535.2 0 85 277 68 535.2 0 68 278 88 535.2 0 88 279 570 535.2 0 570 280 732 535.2 0 732 281 626 535.2 0 626 282 58 535.2 0 58 283 71 535.2 0 71 284 81 535.2 0 81 285 55 535.2 0 55 286 69 535.2 0 69 287 51 535.2 0 51 288 68 535.2 0 68 289 59 535.2 0 59 290 73 535.2 0 73 291 90 535.2 0 90 292 133 535.2 0 133 293 152 535.2 0 152 294 189 535.2 0 189 295 135 535.2 0 135 296 119 535.2 0 119 297 99 535.2 0 99 298 78 535.2 0 78 299 90 535.2 0 90 300 69 535.2 0 69 301 84 535.2 0 84 Finished in 4.66 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 91,212 Reads with adapters: 31,850 (34.9%) Reads that were too short: 43 (0.0%) Reads written (passing filters): 31,807 (34.9%) Total basepairs processed: 24,688,272 bp Total written (filtered): 8,694,943 bp (35.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 31850 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 22 0.0 3 0 0 12 6 4 19 489 0.0 3 0 391 52 35 11 20 30700 0.0 4 28220 1914 396 128 42 21 72 0.0 4 7 58 4 1 2 22 10 0.0 4 1 2 6 1 23 323 0.0 4 216 14 4 61 28 26 2 0.0 4 0 0 0 2 28 1 0.0 4 0 0 0 0 1 29 1 0.0 4 1 38 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 175 0.0 4 0 0 0 0 175 137 2 0.0 4 0 0 0 0 2 141 1 0.0 4 0 0 0 0 1 147 3 0.0 4 0 0 0 0 3 148 3 0.0 4 0 0 0 0 3 163 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 235 3 0.0 4 0 0 0 0 3 259 1 0.0 4 0 0 0 1 263 1 0.0 4 0 0 0 0 1 273 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 282 8 0.0 4 0 0 0 1 7 283 3 0.0 4 0 0 0 0 3 284 2 0.0 4 0 0 0 0 2 289 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.11 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 31,807 Reads with adapters: 4,089 (12.9%) Reads written (passing filters): 31,807 (100.0%) Total basepairs processed: 8,694,943 bp Total written (filtered): 8,637,287 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4089 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.7% C: 8.5% G: 84.0% T: 2.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 159 497.0 0 159 4 27 124.2 0 27 5 1 31.1 0 1 6 1 7.8 0 1 8 1 0.5 0 1 9 28 0.1 0 1 27 10 85 0.0 1 3 82 11 3272 0.0 1 105 3167 12 431 0.0 1 14 417 13 8 0.0 1 0 8 14 2 0.0 1 0 2 15 3 0.0 1 0 3 20 1 0.0 1 0 1 24 1 0.0 1 1 76 1 0.0 1 0 1 152 11 0.0 1 9 2 210 6 0.0 1 4 2 223 1 0.0 1 1 225 47 0.0 1 43 4 228 1 0.0 1 1 259 1 0.0 1 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.