This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 56.19 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 95,560 (4.4%) Reads that were too short: 8,546 (0.4%) Reads written (passing filters): 87,014 (4.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 24,324,564 bp (3.7%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 95560 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1109 535.2 0 1109 7 39469 535.2 0 39469 8 911 535.2 0 911 9 32959 535.2 0 32959 10 84 535.2 0 84 11 53 535.2 0 53 12 28 535.2 0 28 13 38 535.2 0 38 14 42 535.2 0 42 15 39 535.2 0 39 16 31 535.2 0 31 17 53 535.2 0 53 18 54 535.2 0 54 19 44 535.2 0 44 20 51 535.2 0 51 21 45 535.2 0 45 22 52 535.2 0 52 23 375 535.2 0 375 24 267 535.2 0 267 25 442 535.2 0 442 26 38 535.2 0 38 27 17 535.2 0 17 28 34 535.2 0 34 29 23 535.2 0 23 30 31 535.2 0 31 31 27 535.2 0 27 32 34 535.2 0 34 33 42 535.2 0 42 34 29 535.2 0 29 35 32 535.2 0 32 36 29 535.2 0 29 37 26 535.2 0 26 38 30 535.2 0 30 39 36 535.2 0 36 40 24 535.2 0 24 41 28 535.2 0 28 42 24 535.2 0 24 43 43 535.2 0 43 44 36 535.2 0 36 45 27 535.2 0 27 46 36 535.2 0 36 47 34 535.2 0 34 48 20 535.2 0 20 49 25 535.2 0 25 50 28 535.2 0 28 51 36 535.2 0 36 52 21 535.2 0 21 53 20 535.2 0 20 54 25 535.2 0 25 55 30 535.2 0 30 56 29 535.2 0 29 57 26 535.2 0 26 58 21 535.2 0 21 59 18 535.2 0 18 60 33 535.2 0 33 61 28 535.2 0 28 62 20 535.2 0 20 63 28 535.2 0 28 64 32 535.2 0 32 65 13 535.2 0 13 66 23 535.2 0 23 67 15 535.2 0 15 68 24 535.2 0 24 69 30 535.2 0 30 70 31 535.2 0 31 71 31 535.2 0 31 72 17 535.2 0 17 73 44 535.2 0 44 74 62 535.2 0 62 75 27 535.2 0 27 76 31 535.2 0 31 77 26 535.2 0 26 78 37 535.2 0 37 79 17 535.2 0 17 80 17 535.2 0 17 81 25 535.2 0 25 82 30 535.2 0 30 83 29 535.2 0 29 84 51 535.2 0 51 85 23 535.2 0 23 86 48 535.2 0 48 87 53 535.2 0 53 88 453 535.2 0 453 89 314 535.2 0 314 90 317 535.2 0 317 91 23 535.2 0 23 92 26 535.2 0 26 93 20 535.2 0 20 94 29 535.2 0 29 95 26 535.2 0 26 96 47 535.2 0 47 97 49 535.2 0 49 98 51 535.2 0 51 99 54 535.2 0 54 100 45 535.2 0 45 101 278 535.2 0 278 102 526 535.2 0 526 103 530 535.2 0 530 104 45 535.2 0 45 105 36 535.2 0 36 106 32 535.2 0 32 107 15 535.2 0 15 108 20 535.2 0 20 109 24 535.2 0 24 110 32 535.2 0 32 111 31 535.2 0 31 112 31 535.2 0 31 113 41 535.2 0 41 114 48 535.2 0 48 115 19 535.2 0 19 116 29 535.2 0 29 117 44 535.2 0 44 118 17 535.2 0 17 119 39 535.2 0 39 120 41 535.2 0 41 121 40 535.2 0 40 122 49 535.2 0 49 123 32 535.2 0 32 124 53 535.2 0 53 125 60 535.2 0 60 126 181 535.2 0 181 127 1013 535.2 0 1013 128 1063 535.2 0 1063 129 972 535.2 0 972 130 35 535.2 0 35 131 112 535.2 0 112 132 45 535.2 0 45 133 56 535.2 0 56 134 18 535.2 0 18 135 54 535.2 0 54 136 27 535.2 0 27 137 17 535.2 0 17 138 20 535.2 0 20 139 25 535.2 0 25 140 26 535.2 0 26 141 34 535.2 0 34 142 24 535.2 0 24 143 32 535.2 0 32 144 22 535.2 0 22 145 42 535.2 0 42 146 22 535.2 0 22 147 24 535.2 0 24 148 104 535.2 0 104 149 48 535.2 0 48 150 23 535.2 0 23 151 26 535.2 0 26 152 23 535.2 0 23 153 25 535.2 0 25 154 27 535.2 0 27 155 34 535.2 0 34 156 30 535.2 0 30 157 25 535.2 0 25 158 31 535.2 0 31 159 29 535.2 0 29 160 27 535.2 0 27 161 33 535.2 0 33 162 29 535.2 0 29 163 24 535.2 0 24 164 15 535.2 0 15 165 26 535.2 0 26 166 15 535.2 0 15 167 29 535.2 0 29 168 25 535.2 0 25 169 33 535.2 0 33 170 24 535.2 0 24 171 28 535.2 0 28 172 21 535.2 0 21 173 14 535.2 0 14 174 15 535.2 0 15 175 18 535.2 0 18 176 25 535.2 0 25 177 32 535.2 0 32 178 34 535.2 0 34 179 16 535.2 0 16 180 23 535.2 0 23 181 25 535.2 0 25 182 27 535.2 0 27 183 27 535.2 0 27 184 24 535.2 0 24 185 29 535.2 0 29 186 22 535.2 0 22 187 34 535.2 0 34 188 34 535.2 0 34 189 31 535.2 0 31 190 29 535.2 0 29 191 20 535.2 0 20 192 19 535.2 0 19 193 44 535.2 0 44 194 29 535.2 0 29 195 42 535.2 0 42 196 39 535.2 0 39 197 37 535.2 0 37 198 28 535.2 0 28 199 22 535.2 0 22 200 52 535.2 0 52 201 69 535.2 0 69 202 56 535.2 0 56 203 39 535.2 0 39 204 34 535.2 0 34 205 43 535.2 0 43 206 24 535.2 0 24 207 29 535.2 0 29 208 52 535.2 0 52 209 88 535.2 0 88 210 32 535.2 0 32 211 27 535.2 0 27 212 25 535.2 0 25 213 40 535.2 0 40 214 38 535.2 0 38 215 41 535.2 0 41 216 43 535.2 0 43 217 27 535.2 0 27 218 27 535.2 0 27 219 36 535.2 0 36 220 29 535.2 0 29 221 29 535.2 0 29 222 25 535.2 0 25 223 25 535.2 0 25 224 33 535.2 0 33 225 36 535.2 0 36 226 27 535.2 0 27 227 23 535.2 0 23 228 27 535.2 0 27 229 41 535.2 0 41 230 43 535.2 0 43 231 33 535.2 0 33 232 34 535.2 0 34 233 23 535.2 0 23 234 49 535.2 0 49 235 69 535.2 0 69 236 96 535.2 0 96 237 43 535.2 0 43 238 47 535.2 0 47 239 56 535.2 0 56 240 71 535.2 0 71 241 58 535.2 0 58 242 45 535.2 0 45 243 43 535.2 0 43 244 46 535.2 0 46 245 49 535.2 0 49 246 43 535.2 0 43 247 34 535.2 0 34 248 50 535.2 0 50 249 63 535.2 0 63 250 47 535.2 0 47 251 47 535.2 0 47 252 30 535.2 0 30 253 54 535.2 0 54 254 228 535.2 0 228 255 218 535.2 0 218 256 174 535.2 0 174 257 81 535.2 0 81 258 55 535.2 0 55 259 404 535.2 0 404 260 1068 535.2 0 1068 261 625 535.2 0 625 262 384 535.2 0 384 263 39 535.2 0 39 264 29 535.2 0 29 265 26 535.2 0 26 266 32 535.2 0 32 267 21 535.2 0 21 268 31 535.2 0 31 269 27 535.2 0 27 270 32 535.2 0 32 271 20 535.2 0 20 272 33 535.2 0 33 273 40 535.2 0 40 274 32 535.2 0 32 275 37 535.2 0 37 276 51 535.2 0 51 277 51 535.2 0 51 278 48 535.2 0 48 279 587 535.2 0 587 280 619 535.2 0 619 281 409 535.2 0 409 282 49 535.2 0 49 283 35 535.2 0 35 284 32 535.2 0 32 285 29 535.2 0 29 286 37 535.2 0 37 287 30 535.2 0 30 288 41 535.2 0 41 289 23 535.2 0 23 290 34 535.2 0 34 291 38 535.2 0 38 292 61 535.2 0 61 293 59 535.2 0 59 294 121 535.2 0 121 295 77 535.2 0 77 296 89 535.2 0 89 297 75 535.2 0 75 298 42 535.2 0 42 299 26 535.2 0 26 300 46 535.2 0 46 301 32 535.2 0 32 Finished in 4.07 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 87,014 Reads with adapters: 34,233 (39.3%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 34,225 (39.3%) Total basepairs processed: 24,324,564 bp Total written (filtered): 9,405,792 bp (38.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 34233 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 10 0.0 3 0 0 9 1 16 294 0.0 3 0 285 9 17 33766 0.0 3 33133 605 25 3 18 66 0.0 3 2 60 3 1 19 21 0.0 3 0 0 20 1 20 13 0.0 3 4 0 0 9 40 2 0.0 3 0 0 2 42 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 64 24 0.0 3 0 0 0 24 66 2 0.0 3 0 0 0 2 67 3 0.0 3 0 0 0 3 75 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 104 3 0.0 3 0 0 0 3 111 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 1 127 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 155 1 0.0 3 1 159 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 217 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.19 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 34,225 Reads with adapters: 215 (0.6%) Reads written (passing filters): 34,225 (100.0%) Total basepairs processed: 9,405,792 bp Total written (filtered): 9,405,122 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 215 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 67.0% C: 6.0% G: 22.8% T: 4.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 191 534.8 0 191 4 23 133.7 0 23 5 1 33.4 0 1