This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.34 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 288,260 (13.1%) Reads that were too short: 12,751 (0.6%) Reads written (passing filters): 275,509 (12.6%) Total basepairs processed: 659,880,795 bp Total written (filtered): 79,192,779 bp (12.0%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 288260 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1774 535.2 0 1774 7 5494 535.2 0 5494 8 143308 535.2 0 143308 9 110941 535.2 0 110941 10 310 535.2 0 310 11 39 535.2 0 39 12 13 535.2 0 13 13 22 535.2 0 22 14 23 535.2 0 23 15 80 535.2 0 80 16 38 535.2 0 38 17 55 535.2 0 55 18 71 535.2 0 71 19 86 535.2 0 86 20 63 535.2 0 63 21 22 535.2 0 22 22 31 535.2 0 31 23 38 535.2 0 38 24 30 535.2 0 30 25 30 535.2 0 30 26 29 535.2 0 29 27 31 535.2 0 31 28 69 535.2 0 69 29 165 535.2 0 165 30 86 535.2 0 86 31 23 535.2 0 23 32 31 535.2 0 31 33 22 535.2 0 22 34 43 535.2 0 43 35 34 535.2 0 34 36 24 535.2 0 24 37 51 535.2 0 51 38 21 535.2 0 21 39 19 535.2 0 19 40 31 535.2 0 31 41 25 535.2 0 25 42 39 535.2 0 39 43 83 535.2 0 83 44 101 535.2 0 101 45 453 535.2 0 453 46 432 535.2 0 432 47 406 535.2 0 406 48 26 535.2 0 26 49 25 535.2 0 25 50 20 535.2 0 20 51 13 535.2 0 13 52 22 535.2 0 22 53 17 535.2 0 17 54 19 535.2 0 19 55 11 535.2 0 11 56 19 535.2 0 19 57 20 535.2 0 20 58 15 535.2 0 15 59 17 535.2 0 17 60 18 535.2 0 18 61 17 535.2 0 17 62 19 535.2 0 19 63 16 535.2 0 16 64 23 535.2 0 23 65 17 535.2 0 17 66 20 535.2 0 20 67 20 535.2 0 20 68 81 535.2 0 81 69 32 535.2 0 32 70 48 535.2 0 48 71 99 535.2 0 99 72 109 535.2 0 109 73 127 535.2 0 127 74 25 535.2 0 25 75 45 535.2 0 45 76 19 535.2 0 19 77 32 535.2 0 32 78 23 535.2 0 23 79 43 535.2 0 43 80 87 535.2 0 87 81 75 535.2 0 75 82 54 535.2 0 54 83 54 535.2 0 54 84 71 535.2 0 71 85 68 535.2 0 68 86 29 535.2 0 29 87 15 535.2 0 15 88 29 535.2 0 29 89 22 535.2 0 22 90 25 535.2 0 25 91 21 535.2 0 21 92 35 535.2 0 35 93 31 535.2 0 31 94 39 535.2 0 39 95 426 535.2 0 426 96 209 535.2 0 209 97 228 535.2 0 228 98 19 535.2 0 19 99 20 535.2 0 20 100 20 535.2 0 20 101 34 535.2 0 34 102 21 535.2 0 21 103 33 535.2 0 33 104 28 535.2 0 28 105 14 535.2 0 14 106 21 535.2 0 21 107 29 535.2 0 29 108 57 535.2 0 57 109 17 535.2 0 17 110 25 535.2 0 25 111 34 535.2 0 34 112 45 535.2 0 45 113 305 535.2 0 305 114 370 535.2 0 370 115 306 535.2 0 306 116 199 535.2 0 199 117 37 535.2 0 37 118 13 535.2 0 13 119 27 535.2 0 27 120 34 535.2 0 34 121 38 535.2 0 38 122 53 535.2 0 53 123 46 535.2 0 46 124 61 535.2 0 61 125 115 535.2 0 115 126 29 535.2 0 29 127 30 535.2 0 30 128 45 535.2 0 45 129 43 535.2 0 43 130 38 535.2 0 38 131 33 535.2 0 33 132 32 535.2 0 32 133 49 535.2 0 49 134 93 535.2 0 93 135 222 535.2 0 222 136 168 535.2 0 168 137 338 535.2 0 338 138 342 535.2 0 342 139 744 535.2 0 744 140 90 535.2 0 90 141 22 535.2 0 22 142 66 535.2 0 66 143 76 535.2 0 76 144 105 535.2 0 105 145 54 535.2 0 54 146 51 535.2 0 51 147 25 535.2 0 25 148 24 535.2 0 24 149 40 535.2 0 40 150 45 535.2 0 45 151 35 535.2 0 35 152 20 535.2 0 20 153 29 535.2 0 29 154 28 535.2 0 28 155 29 535.2 0 29 156 30 535.2 0 30 157 25 535.2 0 25 158 37 535.2 0 37 159 24 535.2 0 24 160 21 535.2 0 21 161 12 535.2 0 12 162 53 535.2 0 53 163 64 535.2 0 64 164 110 535.2 0 110 165 45 535.2 0 45 166 26 535.2 0 26 167 133 535.2 0 133 168 198 535.2 0 198 169 326 535.2 0 326 170 100 535.2 0 100 171 26 535.2 0 26 172 50 535.2 0 50 173 30 535.2 0 30 174 38 535.2 0 38 175 30 535.2 0 30 176 13 535.2 0 13 177 38 535.2 0 38 178 32 535.2 0 32 179 73 535.2 0 73 180 102 535.2 0 102 181 97 535.2 0 97 182 45 535.2 0 45 183 64 535.2 0 64 184 221 535.2 0 221 185 250 535.2 0 250 186 307 535.2 0 307 187 94 535.2 0 94 188 47 535.2 0 47 189 40 535.2 0 40 190 51 535.2 0 51 191 43 535.2 0 43 192 26 535.2 0 26 193 33 535.2 0 33 194 19 535.2 0 19 195 21 535.2 0 21 196 23 535.2 0 23 197 31 535.2 0 31 198 52 535.2 0 52 199 65 535.2 0 65 200 45 535.2 0 45 201 72 535.2 0 72 202 55 535.2 0 55 203 60 535.2 0 60 204 184 535.2 0 184 205 270 535.2 0 270 206 377 535.2 0 377 207 85 535.2 0 85 208 75 535.2 0 75 209 76 535.2 0 76 210 94 535.2 0 94 211 98 535.2 0 98 212 71 535.2 0 71 213 54 535.2 0 54 214 70 535.2 0 70 215 86 535.2 0 86 216 114 535.2 0 114 217 70 535.2 0 70 218 57 535.2 0 57 219 63 535.2 0 63 220 67 535.2 0 67 221 76 535.2 0 76 222 82 535.2 0 82 223 156 535.2 0 156 224 217 535.2 0 217 225 2315 535.2 0 2315 226 1932 535.2 0 1932 227 2181 535.2 0 2181 228 447 535.2 0 447 229 46 535.2 0 46 230 34 535.2 0 34 231 39 535.2 0 39 232 46 535.2 0 46 233 43 535.2 0 43 234 50 535.2 0 50 235 76 535.2 0 76 236 102 535.2 0 102 237 60 535.2 0 60 238 37 535.2 0 37 239 34 535.2 0 34 240 37 535.2 0 37 241 80 535.2 0 80 242 31 535.2 0 31 243 21 535.2 0 21 244 61 535.2 0 61 245 45 535.2 0 45 246 54 535.2 0 54 247 58 535.2 0 58 248 52 535.2 0 52 249 39 535.2 0 39 250 41 535.2 0 41 251 23 535.2 0 23 252 18 535.2 0 18 253 30 535.2 0 30 254 45 535.2 0 45 255 40 535.2 0 40 256 22 535.2 0 22 257 56 535.2 0 56 258 39 535.2 0 39 259 47 535.2 0 47 260 43 535.2 0 43 261 35 535.2 0 35 262 19 535.2 0 19 263 28 535.2 0 28 264 59 535.2 0 59 265 83 535.2 0 83 266 84 535.2 0 84 267 28 535.2 0 28 268 27 535.2 0 27 269 34 535.2 0 34 270 46 535.2 0 46 271 44 535.2 0 44 272 38 535.2 0 38 273 26 535.2 0 26 274 42 535.2 0 42 275 63 535.2 0 63 276 50 535.2 0 50 277 28 535.2 0 28 278 53 535.2 0 53 279 44 535.2 0 44 280 61 535.2 0 61 281 39 535.2 0 39 282 43 535.2 0 43 283 31 535.2 0 31 284 30 535.2 0 30 285 36 535.2 0 36 286 22 535.2 0 22 287 34 535.2 0 34 288 29 535.2 0 29 289 43 535.2 0 43 290 38 535.2 0 38 291 54 535.2 0 54 292 37 535.2 0 37 293 51 535.2 0 51 294 40 535.2 0 40 295 57 535.2 0 57 296 48 535.2 0 48 297 81 535.2 0 81 298 75 535.2 0 75 299 62 535.2 0 62 300 32 535.2 0 32 301 96 535.2 0 96 Finished in 13.74 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 275,509 Reads with adapters: 121,010 (43.9%) Reads that were too short: 123 (0.0%) Reads written (passing filters): 120,887 (43.9%) Total basepairs processed: 79,192,779 bp Total written (filtered): 32,831,702 bp (41.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 121010 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 34 0.0 3 0 0 0 15 19 18 110 0.0 3 0 0 52 34 24 19 1355 0.0 3 0 1008 135 140 72 20 117675 0.0 4 106911 8096 1926 541 201 21 362 0.0 4 110 200 31 12 9 22 38 0.0 4 6 2 19 4 7 23 728 0.0 4 2 0 0 483 243 24 5 0.0 4 1 0 0 0 4 26 1 0.0 4 1 27 2 0.0 4 0 0 0 0 2 28 1 0.0 4 0 0 0 0 1 33 1 0.0 4 1 34 1 0.0 4 1 35 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 47 2 0.0 4 0 0 0 0 2 50 3 0.0 4 0 0 0 0 3 56 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 89 8 0.0 4 0 0 0 0 8 91 1 0.0 4 0 0 0 0 1 92 3 0.0 4 0 0 0 0 3 103 2 0.0 4 0 0 0 0 2 106 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 1 126 7 0.0 4 0 0 0 0 7 127 520 0.0 4 0 0 0 0 520 128 3 0.0 4 0 0 0 0 3 129 3 0.0 4 0 0 0 0 3 130 2 0.0 4 0 0 0 0 2 132 2 0.0 4 0 0 0 0 2 133 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 1 135 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 1 152 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 166 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 1 190 1 0.0 4 1 196 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 1 204 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 207 4 0.0 4 0 0 0 0 4 208 5 0.0 4 0 1 0 0 4 214 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 0 2 218 1 0.0 4 0 0 0 0 1 225 7 0.0 4 0 0 0 0 7 228 11 0.0 4 0 0 0 0 11 229 2 0.0 4 0 0 0 0 2 230 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 6 0.0 4 0 0 0 1 5 235 7 0.0 4 0 0 0 0 7 237 1 0.0 4 0 0 0 0 1 248 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 1 252 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 257 7 0.0 4 0 0 0 0 7 258 3 0.0 4 0 0 0 0 3 260 2 0.0 4 0 0 0 0 2 262 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 281 2 0.0 4 0 0 0 0 2 282 13 0.0 4 0 0 0 1 12 283 5 0.0 4 0 0 0 1 4 285 3 0.0 4 0 0 0 1 2 288 5 0.0 4 0 0 0 0 5 289 1 0.0 4 0 0 0 0 1 290 4 0.0 4 0 0 0 1 3 292 3 0.0 4 0 0 0 0 3 293 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.47 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 120,887 Reads with adapters: 36,509 (30.2%) Reads written (passing filters): 120,887 (100.0%) Total basepairs processed: 32,831,702 bp Total written (filtered): 32,452,308 bp (98.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 36509 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.1% C: 7.8% G: 87.9% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 59 1888.9 0 59 4 44 472.2 0 44 5 28 118.1 0 28 6 7 29.5 0 7 7 7 7.4 0 7 8 14 1.8 0 14 9 18189 0.5 0 1663 16526 10 15712 0.1 1 1277 14435 11 2038 0.0 1 69 1969 12 152 0.0 1 2 150 13 59 0.0 1 0 59 14 1 0.0 1 0 1 18 1 0.0 1 1 23 1 0.0 1 1 84 1 0.0 1 1 85 5 0.0 1 5 91 1 0.0 1 1 97 1 0.0 1 1 114 1 0.0 1 1 147 1 0.0 1 1 148 2 0.0 1 2 150 100 0.0 1 75 25 151 7 0.0 1 5 2 152 7 0.0 1 5 2 153 3 0.0 1 1 2 155 1 0.0 1 1 158 1 0.0 1 0 1 161 2 0.0 1 1 1 169 1 0.0 1 1 170 1 0.0 1 1 178 2 0.0 1 1 1 189 3 0.0 1 0 3 190 1 0.0 1 0 1 207 1 0.0 1 1 208 35 0.0 1 30 5 209 4 0.0 1 4 210 2 0.0 1 2 211 1 0.0 1 1 220 2 0.0 1 0 2 223 2 0.0 1 2 226 5 0.0 1 4 1 228 3 0.0 1 3 238 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.