This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.11 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 293,655 (13.4%) Reads that were too short: 8,018 (0.4%) Reads written (passing filters): 285,637 (13.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 82,623,961 bp (12.5%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 293655 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1342 535.2 0 1342 7 2910 535.2 0 2910 8 138214 535.2 0 138214 9 134147 535.2 0 134147 10 207 535.2 0 207 11 27 535.2 0 27 12 11 535.2 0 11 13 15 535.2 0 15 14 17 535.2 0 17 15 7 535.2 0 7 16 11 535.2 0 11 17 16 535.2 0 16 18 32 535.2 0 32 19 26 535.2 0 26 20 23 535.2 0 23 21 16 535.2 0 16 22 11 535.2 0 11 23 32 535.2 0 32 24 17 535.2 0 17 25 13 535.2 0 13 26 12 535.2 0 12 27 12 535.2 0 12 28 10 535.2 0 10 29 29 535.2 0 29 30 20 535.2 0 20 31 25 535.2 0 25 32 18 535.2 0 18 33 18 535.2 0 18 34 21 535.2 0 21 35 27 535.2 0 27 36 33 535.2 0 33 37 20 535.2 0 20 38 18 535.2 0 18 39 16 535.2 0 16 40 12 535.2 0 12 41 16 535.2 0 16 42 12 535.2 0 12 43 35 535.2 0 35 44 45 535.2 0 45 45 176 535.2 0 176 46 237 535.2 0 237 47 210 535.2 0 210 48 22 535.2 0 22 49 17 535.2 0 17 50 12 535.2 0 12 51 13 535.2 0 13 52 8 535.2 0 8 53 16 535.2 0 16 54 19 535.2 0 19 55 17 535.2 0 17 56 22 535.2 0 22 57 12 535.2 0 12 58 17 535.2 0 17 59 12 535.2 0 12 60 11 535.2 0 11 61 10 535.2 0 10 62 14 535.2 0 14 63 11 535.2 0 11 64 13 535.2 0 13 65 20 535.2 0 20 66 16 535.2 0 16 67 23 535.2 0 23 68 60 535.2 0 60 69 10 535.2 0 10 70 17 535.2 0 17 71 27 535.2 0 27 72 65 535.2 0 65 73 86 535.2 0 86 74 19 535.2 0 19 75 18 535.2 0 18 76 14 535.2 0 14 77 27 535.2 0 27 78 35 535.2 0 35 79 24 535.2 0 24 80 30 535.2 0 30 81 28 535.2 0 28 82 24 535.2 0 24 83 16 535.2 0 16 84 37 535.2 0 37 85 21 535.2 0 21 86 18 535.2 0 18 87 17 535.2 0 17 88 23 535.2 0 23 89 25 535.2 0 25 90 30 535.2 0 30 91 20 535.2 0 20 92 35 535.2 0 35 93 39 535.2 0 39 94 49 535.2 0 49 95 446 535.2 0 446 96 257 535.2 0 257 97 263 535.2 0 263 98 23 535.2 0 23 99 17 535.2 0 17 100 17 535.2 0 17 101 31 535.2 0 31 102 19 535.2 0 19 103 15 535.2 0 15 104 22 535.2 0 22 105 19 535.2 0 19 106 27 535.2 0 27 107 18 535.2 0 18 108 88 535.2 0 88 109 12 535.2 0 12 110 14 535.2 0 14 111 23 535.2 0 23 112 23 535.2 0 23 113 130 535.2 0 130 114 219 535.2 0 219 115 157 535.2 0 157 116 193 535.2 0 193 117 30 535.2 0 30 118 15 535.2 0 15 119 14 535.2 0 14 120 37 535.2 0 37 121 12 535.2 0 12 122 31 535.2 0 31 123 35 535.2 0 35 124 50 535.2 0 50 125 104 535.2 0 104 126 23 535.2 0 23 127 23 535.2 0 23 128 49 535.2 0 49 129 31 535.2 0 31 130 21 535.2 0 21 131 28 535.2 0 28 132 32 535.2 0 32 133 41 535.2 0 41 134 67 535.2 0 67 135 131 535.2 0 131 136 92 535.2 0 92 137 314 535.2 0 314 138 285 535.2 0 285 139 762 535.2 0 762 140 83 535.2 0 83 141 23 535.2 0 23 142 40 535.2 0 40 143 43 535.2 0 43 144 39 535.2 0 39 145 30 535.2 0 30 146 19 535.2 0 19 147 19 535.2 0 19 148 22 535.2 0 22 149 24 535.2 0 24 150 19 535.2 0 19 151 24 535.2 0 24 152 22 535.2 0 22 153 16 535.2 0 16 154 16 535.2 0 16 155 17 535.2 0 17 156 25 535.2 0 25 157 21 535.2 0 21 158 30 535.2 0 30 159 16 535.2 0 16 160 14 535.2 0 14 161 18 535.2 0 18 162 30 535.2 0 30 163 34 535.2 0 34 164 60 535.2 0 60 165 20 535.2 0 20 166 18 535.2 0 18 167 74 535.2 0 74 168 76 535.2 0 76 169 151 535.2 0 151 170 38 535.2 0 38 171 13 535.2 0 13 172 22 535.2 0 22 173 23 535.2 0 23 174 27 535.2 0 27 175 23 535.2 0 23 176 23 535.2 0 23 177 20 535.2 0 20 178 20 535.2 0 20 179 34 535.2 0 34 180 32 535.2 0 32 181 44 535.2 0 44 182 19 535.2 0 19 183 35 535.2 0 35 184 118 535.2 0 118 185 93 535.2 0 93 186 106 535.2 0 106 187 46 535.2 0 46 188 29 535.2 0 29 189 25 535.2 0 25 190 38 535.2 0 38 191 26 535.2 0 26 192 21 535.2 0 21 193 21 535.2 0 21 194 23 535.2 0 23 195 21 535.2 0 21 196 13 535.2 0 13 197 23 535.2 0 23 198 34 535.2 0 34 199 43 535.2 0 43 200 16 535.2 0 16 201 30 535.2 0 30 202 29 535.2 0 29 203 22 535.2 0 22 204 72 535.2 0 72 205 89 535.2 0 89 206 123 535.2 0 123 207 40 535.2 0 40 208 30 535.2 0 30 209 28 535.2 0 28 210 39 535.2 0 39 211 45 535.2 0 45 212 41 535.2 0 41 213 26 535.2 0 26 214 34 535.2 0 34 215 46 535.2 0 46 216 50 535.2 0 50 217 32 535.2 0 32 218 41 535.2 0 41 219 39 535.2 0 39 220 44 535.2 0 44 221 41 535.2 0 41 222 50 535.2 0 50 223 82 535.2 0 82 224 155 535.2 0 155 225 1885 535.2 0 1885 226 1362 535.2 0 1362 227 1310 535.2 0 1310 228 267 535.2 0 267 229 19 535.2 0 19 230 31 535.2 0 31 231 27 535.2 0 27 232 28 535.2 0 28 233 23 535.2 0 23 234 25 535.2 0 25 235 39 535.2 0 39 236 49 535.2 0 49 237 33 535.2 0 33 238 16 535.2 0 16 239 22 535.2 0 22 240 23 535.2 0 23 241 26 535.2 0 26 242 20 535.2 0 20 243 24 535.2 0 24 244 34 535.2 0 34 245 22 535.2 0 22 246 33 535.2 0 33 247 21 535.2 0 21 248 22 535.2 0 22 249 32 535.2 0 32 250 30 535.2 0 30 251 22 535.2 0 22 252 23 535.2 0 23 253 26 535.2 0 26 254 34 535.2 0 34 255 23 535.2 0 23 256 29 535.2 0 29 257 33 535.2 0 33 258 29 535.2 0 29 259 28 535.2 0 28 260 26 535.2 0 26 261 17 535.2 0 17 262 27 535.2 0 27 263 18 535.2 0 18 264 28 535.2 0 28 265 32 535.2 0 32 266 25 535.2 0 25 267 20 535.2 0 20 268 21 535.2 0 21 269 42 535.2 0 42 270 24 535.2 0 24 271 17 535.2 0 17 272 26 535.2 0 26 273 20 535.2 0 20 274 25 535.2 0 25 275 23 535.2 0 23 276 20 535.2 0 20 277 22 535.2 0 22 278 38 535.2 0 38 279 30 535.2 0 30 280 43 535.2 0 43 281 28 535.2 0 28 282 22 535.2 0 22 283 24 535.2 0 24 284 23 535.2 0 23 285 32 535.2 0 32 286 24 535.2 0 24 287 16 535.2 0 16 288 25 535.2 0 25 289 22 535.2 0 22 290 25 535.2 0 25 291 30 535.2 0 30 292 20 535.2 0 20 293 28 535.2 0 28 294 31 535.2 0 31 295 29 535.2 0 29 296 29 535.2 0 29 297 33 535.2 0 33 298 34 535.2 0 34 299 36 535.2 0 36 300 17 535.2 0 17 301 78 535.2 0 78 Finished in 12.44 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 285,637 Reads with adapters: 133,378 (46.7%) Reads that were too short: 27 (0.0%) Reads written (passing filters): 133,351 (46.7%) Total basepairs processed: 82,623,961 bp Total written (filtered): 36,802,591 bp (44.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 133378 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 23 0.0 3 0 0 22 1 16 732 0.0 3 0 718 14 17 132173 0.0 3 129712 2371 81 9 18 301 0.0 3 61 233 7 19 59 0.0 3 0 1 52 6 20 24 0.0 3 1 0 1 22 21 1 0.0 3 0 0 0 1 22 1 0.0 3 1 24 3 0.0 3 0 0 0 3 27 1 0.0 3 1 29 1 0.0 3 1 35 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 56 6 0.0 3 0 0 0 6 58 1 0.0 3 0 0 1 68 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 4 0.0 3 0 0 0 4 118 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 4 0.0 3 0 0 0 4 127 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 162 1 0.0 3 1 174 1 0.0 3 1 179 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 191 1 0.0 3 0 0 0 1 238 3 0.0 3 0 0 0 3 247 1 0.0 3 1 254 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 5 0.0 3 0 0 1 4 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.64 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 133,351 Reads with adapters: 340 (0.3%) Reads written (passing filters): 133,351 (100.0%) Total basepairs processed: 36,802,591 bp Total written (filtered): 36,801,461 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 340 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.3% C: 10.0% G: 28.8% T: 5.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 248 2083.6 0 248 4 75 520.9 0 75 5 16 130.2 0 16 6 1 32.6 0 1