This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 59.17 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 476,681 (21.7%) Reads that were too short: 11,442 (0.5%) Reads written (passing filters): 465,239 (21.2%) Total basepairs processed: 659,880,795 bp Total written (filtered): 91,560,894 bp (13.9%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 476681 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1234 535.2 0 1234 7 63664 535.2 0 63664 8 51314 535.2 0 51314 9 126 535.2 0 126 10 108 535.2 0 108 11 95 535.2 0 95 12 101 535.2 0 101 13 76 535.2 0 76 14 79 535.2 0 79 15 96 535.2 0 96 16 68 535.2 0 68 17 81 535.2 0 81 18 59 535.2 0 59 19 74 535.2 0 74 20 67 535.2 0 67 21 75 535.2 0 75 22 97 535.2 0 97 23 103 535.2 0 103 24 90 535.2 0 90 25 165 535.2 0 165 26 211 535.2 0 211 27 248 535.2 0 248 28 84 535.2 0 84 29 92 535.2 0 92 30 94 535.2 0 94 31 58 535.2 0 58 32 79 535.2 0 79 33 58 535.2 0 58 34 171 535.2 0 171 35 108 535.2 0 108 36 102 535.2 0 102 37 103 535.2 0 103 38 71 535.2 0 71 39 54 535.2 0 54 40 68 535.2 0 68 41 65 535.2 0 65 42 86 535.2 0 86 43 102 535.2 0 102 44 57 535.2 0 57 45 65 535.2 0 65 46 89 535.2 0 89 47 56 535.2 0 56 48 74 535.2 0 74 49 80 535.2 0 80 50 132 535.2 0 132 51 81 535.2 0 81 52 72 535.2 0 72 53 80 535.2 0 80 54 93 535.2 0 93 55 75 535.2 0 75 56 91 535.2 0 91 57 90 535.2 0 90 58 89 535.2 0 89 59 77 535.2 0 77 60 98 535.2 0 98 61 106 535.2 0 106 62 112 535.2 0 112 63 103 535.2 0 103 64 118 535.2 0 118 65 81 535.2 0 81 66 106 535.2 0 106 67 114 535.2 0 114 68 151 535.2 0 151 69 141 535.2 0 141 70 133 535.2 0 133 71 103 535.2 0 103 72 210 535.2 0 210 73 233 535.2 0 233 74 303 535.2 0 303 75 425 535.2 0 425 76 217 535.2 0 217 77 242 535.2 0 242 78 332 535.2 0 332 79 1659 535.2 0 1659 80 1066 535.2 0 1066 81 1761 535.2 0 1761 82 794 535.2 0 794 83 1120 535.2 0 1120 84 276 535.2 0 276 85 331 535.2 0 331 86 342 535.2 0 342 87 111 535.2 0 111 88 120 535.2 0 120 89 121 535.2 0 121 90 104 535.2 0 104 91 105 535.2 0 105 92 107 535.2 0 107 93 151 535.2 0 151 94 185 535.2 0 185 95 180 535.2 0 180 96 165 535.2 0 165 97 139 535.2 0 139 98 148 535.2 0 148 99 117 535.2 0 117 100 179 535.2 0 179 101 240 535.2 0 240 102 204 535.2 0 204 103 225 535.2 0 225 104 256 535.2 0 256 105 504 535.2 0 504 106 221 535.2 0 221 107 306 535.2 0 306 108 497 535.2 0 497 109 298 535.2 0 298 110 310 535.2 0 310 111 506 535.2 0 506 112 423 535.2 0 423 113 510 535.2 0 510 114 475 535.2 0 475 115 761 535.2 0 761 116 910 535.2 0 910 117 1365 535.2 0 1365 118 14471 535.2 0 14471 119 22168 535.2 0 22168 120 22042 535.2 0 22042 121 4780 535.2 0 4780 122 5522 535.2 0 5522 123 3605 535.2 0 3605 124 612 535.2 0 612 125 391 535.2 0 391 126 613 535.2 0 613 127 493 535.2 0 493 128 401 535.2 0 401 129 392 535.2 0 392 130 500 535.2 0 500 131 831 535.2 0 831 132 1343 535.2 0 1343 133 728 535.2 0 728 134 772 535.2 0 772 135 1192 535.2 0 1192 136 837 535.2 0 837 137 1219 535.2 0 1219 138 1714 535.2 0 1714 139 3903 535.2 0 3903 140 8644 535.2 0 8644 141 26671 535.2 0 26671 142 15177 535.2 0 15177 143 2498 535.2 0 2498 144 3184 535.2 0 3184 145 26957 535.2 0 26957 146 53621 535.2 0 53621 147 58999 535.2 0 58999 148 7942 535.2 0 7942 149 2826 535.2 0 2826 150 831 535.2 0 831 151 462 535.2 0 462 152 407 535.2 0 407 153 272 535.2 0 272 154 324 535.2 0 324 155 347 535.2 0 347 156 286 535.2 0 286 157 299 535.2 0 299 158 492 535.2 0 492 159 552 535.2 0 552 160 1601 535.2 0 1601 161 324 535.2 0 324 162 474 535.2 0 474 163 690 535.2 0 690 164 753 535.2 0 753 165 4273 535.2 0 4273 166 7136 535.2 0 7136 167 4231 535.2 0 4231 168 422 535.2 0 422 169 286 535.2 0 286 170 153 535.2 0 153 171 103 535.2 0 103 172 114 535.2 0 114 173 125 535.2 0 125 174 132 535.2 0 132 175 125 535.2 0 125 176 88 535.2 0 88 177 110 535.2 0 110 178 100 535.2 0 100 179 99 535.2 0 99 180 94 535.2 0 94 181 157 535.2 0 157 182 129 535.2 0 129 183 108 535.2 0 108 184 139 535.2 0 139 185 102 535.2 0 102 186 115 535.2 0 115 187 110 535.2 0 110 188 94 535.2 0 94 189 104 535.2 0 104 190 106 535.2 0 106 191 119 535.2 0 119 192 174 535.2 0 174 193 291 535.2 0 291 194 317 535.2 0 317 195 137 535.2 0 137 196 93 535.2 0 93 197 124 535.2 0 124 198 104 535.2 0 104 199 132 535.2 0 132 200 153 535.2 0 153 201 131 535.2 0 131 202 111 535.2 0 111 203 95 535.2 0 95 204 95 535.2 0 95 205 109 535.2 0 109 206 144 535.2 0 144 207 126 535.2 0 126 208 112 535.2 0 112 209 138 535.2 0 138 210 128 535.2 0 128 211 128 535.2 0 128 212 65 535.2 0 65 213 97 535.2 0 97 214 87 535.2 0 87 215 98 535.2 0 98 216 268 535.2 0 268 217 423 535.2 0 423 218 344 535.2 0 344 219 260 535.2 0 260 220 109 535.2 0 109 221 101 535.2 0 101 222 79 535.2 0 79 223 108 535.2 0 108 224 101 535.2 0 101 225 129 535.2 0 129 226 149 535.2 0 149 227 127 535.2 0 127 228 104 535.2 0 104 229 69 535.2 0 69 230 88 535.2 0 88 231 85 535.2 0 85 232 73 535.2 0 73 233 78 535.2 0 78 234 102 535.2 0 102 235 80 535.2 0 80 236 87 535.2 0 87 237 109 535.2 0 109 238 88 535.2 0 88 239 85 535.2 0 85 240 87 535.2 0 87 241 99 535.2 0 99 242 84 535.2 0 84 243 99 535.2 0 99 244 111 535.2 0 111 245 93 535.2 0 93 246 118 535.2 0 118 247 152 535.2 0 152 248 140 535.2 0 140 249 86 535.2 0 86 250 92 535.2 0 92 251 72 535.2 0 72 252 87 535.2 0 87 253 101 535.2 0 101 254 112 535.2 0 112 255 110 535.2 0 110 256 108 535.2 0 108 257 89 535.2 0 89 258 91 535.2 0 91 259 93 535.2 0 93 260 99 535.2 0 99 261 83 535.2 0 83 262 100 535.2 0 100 263 89 535.2 0 89 264 107 535.2 0 107 265 145 535.2 0 145 266 122 535.2 0 122 267 134 535.2 0 134 268 82 535.2 0 82 269 64 535.2 0 64 270 119 535.2 0 119 271 91 535.2 0 91 272 95 535.2 0 95 273 139 535.2 0 139 274 128 535.2 0 128 275 128 535.2 0 128 276 91 535.2 0 91 277 117 535.2 0 117 278 110 535.2 0 110 279 117 535.2 0 117 280 95 535.2 0 95 281 100 535.2 0 100 282 88 535.2 0 88 283 82 535.2 0 82 284 73 535.2 0 73 285 93 535.2 0 93 286 90 535.2 0 90 287 93 535.2 0 93 288 119 535.2 0 119 289 98 535.2 0 98 290 109 535.2 0 109 291 116 535.2 0 116 292 93 535.2 0 93 293 86 535.2 0 86 294 99 535.2 0 99 295 105 535.2 0 105 296 108 535.2 0 108 297 127 535.2 0 127 298 121 535.2 0 121 299 144 535.2 0 144 300 157 535.2 0 157 301 283 535.2 0 283 Finished in 18.58 s (40 us/read; 1.50 M reads/minute). === Summary === Total reads processed: 465,239 Reads with adapters: 54,328 (11.7%) Reads that were too short: 224 (0.0%) Reads written (passing filters): 54,104 (11.6%) Total basepairs processed: 91,560,894 bp Total written (filtered): 14,613,841 bp (16.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 54328 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 12 0.0 3 0 0 0 1 11 18 29 0.0 3 0 0 18 6 5 19 810 0.0 3 0 599 110 67 34 20 51385 0.0 4 47039 3352 727 209 58 21 139 0.0 4 24 98 10 4 3 22 36 0.0 4 7 2 15 7 5 23 203 0.0 4 2 1 1 130 69 24 1 0.0 4 0 0 0 0 1 25 4 0.0 4 1 0 0 1 2 26 3 0.0 4 3 27 1 0.0 4 0 0 0 0 1 39 2 0.0 4 0 0 0 1 1 41 8 0.0 4 0 0 0 0 8 43 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 50 7 0.0 4 0 0 0 0 7 52 1 0.0 4 1 53 13 0.0 4 0 0 0 0 13 55 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 67 6 0.0 4 0 0 0 0 6 70 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 73 2 0.0 4 0 0 0 0 2 75 9 0.0 4 0 0 0 0 9 76 8 0.0 4 0 0 0 0 8 77 7 0.0 4 0 0 0 0 7 79 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 85 3 0.0 4 0 0 0 0 3 86 1 0.0 4 0 0 0 0 1 87 2 0.0 4 0 0 0 0 2 89 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 97 5 0.0 4 0 0 0 0 5 98 2 0.0 4 0 0 0 0 2 100 7 0.0 4 0 0 0 0 7 102 3 0.0 4 0 0 0 0 3 107 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 0 2 109 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 113 2 0.0 4 0 0 0 1 1 114 1 0.0 4 0 0 0 0 1 116 3 0.0 4 0 0 0 0 3 117 1 0.0 4 0 0 1 118 2 0.0 4 0 0 0 0 2 119 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 2 0.0 4 0 0 0 0 2 123 5 0.0 4 0 0 0 0 5 124 15 0.0 4 0 0 0 0 15 125 7 0.0 4 0 0 0 1 6 126 19 0.0 4 0 0 0 0 19 127 1402 0.0 4 0 0 0 1 1401 128 5 0.0 4 0 0 0 0 5 129 10 0.0 4 0 0 0 0 10 130 4 0.0 4 0 0 0 0 4 131 1 0.0 4 0 0 0 0 1 133 2 0.0 4 0 0 0 1 1 134 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 137 2 0.0 4 0 0 0 1 1 140 1 0.0 4 0 0 0 0 1 143 2 0.0 4 0 0 0 0 2 145 1 0.0 4 0 0 0 0 1 146 4 0.0 4 0 0 0 0 4 147 2 0.0 4 0 0 0 0 2 148 2 0.0 4 0 0 0 0 2 149 1 0.0 4 0 0 0 0 1 152 3 0.0 4 0 0 0 0 3 154 1 0.0 4 0 0 0 0 1 155 11 0.0 4 0 0 0 0 11 156 4 0.0 4 0 0 0 0 4 157 3 0.0 4 0 0 0 0 3 158 2 0.0 4 0 0 0 0 2 159 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 169 2 0.0 4 0 0 0 0 2 172 1 0.0 4 0 0 0 0 1 177 8 0.0 4 0 0 0 0 8 178 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 180 1 0.0 4 1 181 2 0.0 4 0 0 0 0 2 182 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 202 2 0.0 4 0 0 0 0 2 204 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 1 225 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 1 5 229 3 0.0 4 0 0 0 0 3 230 2 0.0 4 0 0 0 0 2 233 2 0.0 4 0 0 0 0 2 234 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 236 2 0.0 4 0 0 0 0 2 238 1 0.0 4 0 0 0 0 1 241 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 5 0.0 4 0 0 0 0 5 283 3 0.0 4 0 0 0 0 3 285 1 0.0 4 0 0 0 0 1 288 3 0.0 4 0 0 0 0 3 289 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.95 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 54,104 Reads with adapters: 12,047 (22.3%) Reads written (passing filters): 54,104 (100.0%) Total basepairs processed: 14,613,841 bp Total written (filtered): 14,471,248 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 12047 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 8.5% G: 86.5% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 107 845.4 0 107 4 183 211.3 0 183 5 5 52.8 0 5 6 49 13.2 0 49 7 5 3.3 0 5 8 1 0.8 0 1 9 136 0.2 0 11 125 10 2939 0.1 1 228 2711 11 8421 0.0 1 349 8072 12 73 0.0 1 3 70 13 17 0.0 1 0 17 14 7 0.0 1 0 7 148 2 0.0 1 2 151 80 0.0 1 47 33 152 2 0.0 1 2 170 1 0.0 1 1 178 3 0.0 1 0 3 184 1 0.0 1 1 209 11 0.0 1 8 3 210 2 0.0 1 2 224 1 0.0 1 1 227 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.