This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.77 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 472,609 (21.6%) Reads that were too short: 11,677 (0.5%) Reads written (passing filters): 460,932 (21.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 91,746,513 bp (13.9%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 472609 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 697 535.2 0 697 7 57286 535.2 0 57286 8 65345 535.2 0 65345 9 116 535.2 0 116 10 100 535.2 0 100 11 75 535.2 0 75 12 93 535.2 0 93 13 73 535.2 0 73 14 97 535.2 0 97 15 85 535.2 0 85 16 73 535.2 0 73 17 56 535.2 0 56 18 81 535.2 0 81 19 67 535.2 0 67 20 77 535.2 0 77 21 74 535.2 0 74 22 61 535.2 0 61 23 73 535.2 0 73 24 80 535.2 0 80 25 73 535.2 0 73 26 125 535.2 0 125 27 96 535.2 0 96 28 71 535.2 0 71 29 96 535.2 0 96 30 128 535.2 0 128 31 84 535.2 0 84 32 67 535.2 0 67 33 63 535.2 0 63 34 148 535.2 0 148 35 98 535.2 0 98 36 88 535.2 0 88 37 137 535.2 0 137 38 102 535.2 0 102 39 92 535.2 0 92 40 109 535.2 0 109 41 78 535.2 0 78 42 107 535.2 0 107 43 80 535.2 0 80 44 74 535.2 0 74 45 80 535.2 0 80 46 91 535.2 0 91 47 77 535.2 0 77 48 77 535.2 0 77 49 98 535.2 0 98 50 167 535.2 0 167 51 94 535.2 0 94 52 106 535.2 0 106 53 88 535.2 0 88 54 102 535.2 0 102 55 97 535.2 0 97 56 84 535.2 0 84 57 75 535.2 0 75 58 100 535.2 0 100 59 73 535.2 0 73 60 95 535.2 0 95 61 121 535.2 0 121 62 123 535.2 0 123 63 117 535.2 0 117 64 114 535.2 0 114 65 89 535.2 0 89 66 114 535.2 0 114 67 93 535.2 0 93 68 162 535.2 0 162 69 126 535.2 0 126 70 186 535.2 0 186 71 90 535.2 0 90 72 108 535.2 0 108 73 95 535.2 0 95 74 126 535.2 0 126 75 258 535.2 0 258 76 132 535.2 0 132 77 181 535.2 0 181 78 260 535.2 0 260 79 1677 535.2 0 1677 80 1029 535.2 0 1029 81 1435 535.2 0 1435 82 449 535.2 0 449 83 586 535.2 0 586 84 209 535.2 0 209 85 314 535.2 0 314 86 330 535.2 0 330 87 138 535.2 0 138 88 120 535.2 0 120 89 167 535.2 0 167 90 137 535.2 0 137 91 115 535.2 0 115 92 135 535.2 0 135 93 169 535.2 0 169 94 202 535.2 0 202 95 211 535.2 0 211 96 200 535.2 0 200 97 134 535.2 0 134 98 168 535.2 0 168 99 159 535.2 0 159 100 155 535.2 0 155 101 245 535.2 0 245 102 263 535.2 0 263 103 261 535.2 0 261 104 307 535.2 0 307 105 576 535.2 0 576 106 262 535.2 0 262 107 315 535.2 0 315 108 559 535.2 0 559 109 303 535.2 0 303 110 330 535.2 0 330 111 558 535.2 0 558 112 476 535.2 0 476 113 465 535.2 0 465 114 429 535.2 0 429 115 704 535.2 0 704 116 765 535.2 0 765 117 1148 535.2 0 1148 118 15123 535.2 0 15123 119 23528 535.2 0 23528 120 22022 535.2 0 22022 121 4628 535.2 0 4628 122 4930 535.2 0 4930 123 2827 535.2 0 2827 124 658 535.2 0 658 125 434 535.2 0 434 126 614 535.2 0 614 127 535 535.2 0 535 128 428 535.2 0 428 129 422 535.2 0 422 130 519 535.2 0 519 131 778 535.2 0 778 132 1221 535.2 0 1221 133 758 535.2 0 758 134 751 535.2 0 751 135 1061 535.2 0 1061 136 747 535.2 0 747 137 1084 535.2 0 1084 138 1505 535.2 0 1505 139 3892 535.2 0 3892 140 8527 535.2 0 8527 141 30793 535.2 0 30793 142 16826 535.2 0 16826 143 2131 535.2 0 2131 144 2423 535.2 0 2423 145 23090 535.2 0 23090 146 47622 535.2 0 47622 147 52060 535.2 0 52060 148 8865 535.2 0 8865 149 3196 535.2 0 3196 150 540 535.2 0 540 151 428 535.2 0 428 152 463 535.2 0 463 153 326 535.2 0 326 154 376 535.2 0 376 155 535 535.2 0 535 156 290 535.2 0 290 157 348 535.2 0 348 158 694 535.2 0 694 159 750 535.2 0 750 160 2210 535.2 0 2210 161 375 535.2 0 375 162 371 535.2 0 371 163 398 535.2 0 398 164 561 535.2 0 561 165 4665 535.2 0 4665 166 7999 535.2 0 7999 167 4364 535.2 0 4364 168 297 535.2 0 297 169 155 535.2 0 155 170 103 535.2 0 103 171 115 535.2 0 115 172 123 535.2 0 123 173 115 535.2 0 115 174 137 535.2 0 137 175 146 535.2 0 146 176 113 535.2 0 113 177 123 535.2 0 123 178 111 535.2 0 111 179 116 535.2 0 116 180 109 535.2 0 109 181 128 535.2 0 128 182 144 535.2 0 144 183 133 535.2 0 133 184 120 535.2 0 120 185 122 535.2 0 122 186 121 535.2 0 121 187 91 535.2 0 91 188 119 535.2 0 119 189 102 535.2 0 102 190 109 535.2 0 109 191 107 535.2 0 107 192 123 535.2 0 123 193 153 535.2 0 153 194 200 535.2 0 200 195 124 535.2 0 124 196 121 535.2 0 121 197 147 535.2 0 147 198 112 535.2 0 112 199 114 535.2 0 114 200 175 535.2 0 175 201 184 535.2 0 184 202 113 535.2 0 113 203 107 535.2 0 107 204 145 535.2 0 145 205 133 535.2 0 133 206 128 535.2 0 128 207 128 535.2 0 128 208 135 535.2 0 135 209 138 535.2 0 138 210 131 535.2 0 131 211 114 535.2 0 114 212 104 535.2 0 104 213 95 535.2 0 95 214 111 535.2 0 111 215 115 535.2 0 115 216 264 535.2 0 264 217 413 535.2 0 413 218 327 535.2 0 327 219 269 535.2 0 269 220 100 535.2 0 100 221 141 535.2 0 141 222 90 535.2 0 90 223 97 535.2 0 97 224 99 535.2 0 99 225 119 535.2 0 119 226 143 535.2 0 143 227 95 535.2 0 95 228 82 535.2 0 82 229 80 535.2 0 80 230 128 535.2 0 128 231 102 535.2 0 102 232 74 535.2 0 74 233 93 535.2 0 93 234 111 535.2 0 111 235 90 535.2 0 90 236 101 535.2 0 101 237 99 535.2 0 99 238 98 535.2 0 98 239 106 535.2 0 106 240 96 535.2 0 96 241 118 535.2 0 118 242 93 535.2 0 93 243 90 535.2 0 90 244 97 535.2 0 97 245 88 535.2 0 88 246 133 535.2 0 133 247 108 535.2 0 108 248 115 535.2 0 115 249 86 535.2 0 86 250 79 535.2 0 79 251 72 535.2 0 72 252 81 535.2 0 81 253 91 535.2 0 91 254 98 535.2 0 98 255 122 535.2 0 122 256 115 535.2 0 115 257 113 535.2 0 113 258 105 535.2 0 105 259 97 535.2 0 97 260 93 535.2 0 93 261 121 535.2 0 121 262 130 535.2 0 130 263 112 535.2 0 112 264 92 535.2 0 92 265 118 535.2 0 118 266 117 535.2 0 117 267 118 535.2 0 118 268 78 535.2 0 78 269 109 535.2 0 109 270 97 535.2 0 97 271 116 535.2 0 116 272 85 535.2 0 85 273 93 535.2 0 93 274 103 535.2 0 103 275 104 535.2 0 104 276 96 535.2 0 96 277 91 535.2 0 91 278 110 535.2 0 110 279 92 535.2 0 92 280 89 535.2 0 89 281 114 535.2 0 114 282 103 535.2 0 103 283 88 535.2 0 88 284 107 535.2 0 107 285 105 535.2 0 105 286 93 535.2 0 93 287 104 535.2 0 104 288 132 535.2 0 132 289 97 535.2 0 97 290 104 535.2 0 104 291 116 535.2 0 116 292 109 535.2 0 109 293 119 535.2 0 119 294 130 535.2 0 130 295 112 535.2 0 112 296 106 535.2 0 106 297 132 535.2 0 132 298 100 535.2 0 100 299 105 535.2 0 105 300 126 535.2 0 126 301 296 535.2 0 296 Finished in 16.26 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 460,932 Reads with adapters: 59,743 (13.0%) Reads that were too short: 275 (0.1%) Reads written (passing filters): 59,468 (12.9%) Total basepairs processed: 91,746,513 bp Total written (filtered): 16,030,456 bp (17.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 59743 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 8 0.0 3 0 0 8 16 300 0.0 3 0 290 10 17 55266 0.0 3 54193 1046 24 3 18 121 0.0 3 8 111 1 1 19 994 0.0 3 965 11 16 2 20 16 0.0 3 1 2 0 13 21 1 0.0 3 0 0 1 23 11 0.0 3 0 0 0 11 25 1 0.0 3 0 0 0 1 26 1085 0.0 3 0 0 5 1080 27 9 0.0 3 0 0 1 8 29 133 0.0 3 0 0 2 131 30 2 0.0 3 0 0 0 2 32 2 0.0 3 0 0 0 2 37 1 0.0 3 1 39 1 0.0 3 0 0 1 40 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 4 0.0 3 0 0 0 4 48 5 0.0 3 0 0 0 5 49 2 0.0 3 0 0 0 2 50 31 0.0 3 0 0 1 30 51 2 0.0 3 0 0 0 2 52 15 0.0 3 0 0 0 15 53 50 0.0 3 0 0 1 49 56 1 0.0 3 1 57 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 1 1 68 597 0.0 3 0 0 5 592 69 1 0.0 3 0 0 1 71 2 0.0 3 0 0 0 2 72 144 0.0 3 0 0 0 144 73 53 0.0 3 0 0 0 53 74 542 0.0 3 0 0 2 540 76 6 0.0 3 0 0 0 6 77 36 0.0 3 0 0 0 36 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 2 0.0 3 1 0 0 1 85 3 0.0 3 0 0 0 3 86 6 0.0 3 0 0 0 6 88 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 1 0.0 3 1 92 1 0.0 3 0 0 0 1 95 4 0.0 3 0 0 0 4 96 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 5 0.0 3 0 0 0 5 111 6 0.0 3 0 0 0 6 112 2 0.0 3 0 0 0 2 113 2 0.0 3 0 0 0 2 114 3 0.0 3 0 0 0 3 115 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 3 0.0 3 0 0 0 3 127 7 0.0 3 1 0 0 6 128 9 0.0 3 0 0 3 6 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 5 0.0 3 0 0 0 5 134 3 0.0 3 1 0 0 2 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 138 57 0.0 3 0 0 0 57 139 3 0.0 3 0 0 0 3 140 7 0.0 3 0 0 0 7 141 47 0.0 3 0 0 2 45 142 1 0.0 3 0 0 0 1 146 4 0.0 3 0 0 0 4 147 3 0.0 3 0 0 0 3 149 3 0.0 3 0 0 0 3 151 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 155 1 0.0 3 0 1 156 22 0.0 3 0 0 0 22 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 11 0.0 3 0 0 0 11 169 2 0.0 3 0 0 0 2 170 2 0.0 3 0 0 0 2 171 3 0.0 3 0 0 0 3 172 3 0.0 3 0 0 0 3 174 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 196 2 0.0 3 0 0 0 2 207 2 0.0 3 0 0 0 2 213 1 0.0 3 0 0 0 1 229 1 0.0 3 1 254 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.06 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 59,468 Reads with adapters: 125 (0.2%) Reads written (passing filters): 59,468 (100.0%) Total basepairs processed: 16,030,456 bp Total written (filtered): 16,030,074 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 125 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 32.8% C: 28.8% G: 18.4% T: 20.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 120 929.2 0 120 4 4 232.3 0 4 6 1 14.5 0 1