This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.84 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 247,564 (11.3%) Reads that were too short: 9,410 (0.4%) Reads written (passing filters): 238,154 (10.9%) Total basepairs processed: 659,880,795 bp Total written (filtered): 64,334,526 bp (9.7%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 247564 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 8752 535.2 0 8752 7 104434 535.2 0 104434 8 4413 535.2 0 4413 9 82160 535.2 0 82160 10 67 535.2 0 67 11 36 535.2 0 36 12 41 535.2 0 41 13 44 535.2 0 44 14 35 535.2 0 35 15 33 535.2 0 33 16 42 535.2 0 42 17 41 535.2 0 41 18 39 535.2 0 39 19 41 535.2 0 41 20 48 535.2 0 48 21 42 535.2 0 42 22 53 535.2 0 53 23 39 535.2 0 39 24 38 535.2 0 38 25 27 535.2 0 27 26 33 535.2 0 33 27 42 535.2 0 42 28 49 535.2 0 49 29 26 535.2 0 26 30 80 535.2 0 80 31 136 535.2 0 136 32 46 535.2 0 46 33 235 535.2 0 235 34 223 535.2 0 223 35 362 535.2 0 362 36 33 535.2 0 33 37 41 535.2 0 41 38 36 535.2 0 36 39 57 535.2 0 57 40 34 535.2 0 34 41 41 535.2 0 41 42 27 535.2 0 27 43 40 535.2 0 40 44 49 535.2 0 49 45 52 535.2 0 52 46 57 535.2 0 57 47 47 535.2 0 47 48 53 535.2 0 53 49 54 535.2 0 54 50 39 535.2 0 39 51 29 535.2 0 29 52 30 535.2 0 30 53 42 535.2 0 42 54 29 535.2 0 29 55 38 535.2 0 38 56 50 535.2 0 50 57 82 535.2 0 82 58 84 535.2 0 84 59 58 535.2 0 58 60 58 535.2 0 58 61 41 535.2 0 41 62 35 535.2 0 35 63 21 535.2 0 21 64 51 535.2 0 51 65 57 535.2 0 57 66 28 535.2 0 28 67 24 535.2 0 24 68 42 535.2 0 42 69 29 535.2 0 29 70 40 535.2 0 40 71 42 535.2 0 42 72 107 535.2 0 107 73 434 535.2 0 434 74 122 535.2 0 122 75 32 535.2 0 32 76 41 535.2 0 41 77 39 535.2 0 39 78 42 535.2 0 42 79 38 535.2 0 38 80 31 535.2 0 31 81 26 535.2 0 26 82 35 535.2 0 35 83 41 535.2 0 41 84 133 535.2 0 133 85 83 535.2 0 83 86 164 535.2 0 164 87 106 535.2 0 106 88 43 535.2 0 43 89 37 535.2 0 37 90 53 535.2 0 53 91 42 535.2 0 42 92 35 535.2 0 35 93 50 535.2 0 50 94 66 535.2 0 66 95 42 535.2 0 42 96 46 535.2 0 46 97 53 535.2 0 53 98 60 535.2 0 60 99 320 535.2 0 320 100 51 535.2 0 51 101 53 535.2 0 53 102 95 535.2 0 95 103 51 535.2 0 51 104 52 535.2 0 52 105 51 535.2 0 51 106 203 535.2 0 203 107 660 535.2 0 660 108 1581 535.2 0 1581 109 692 535.2 0 692 110 41 535.2 0 41 111 42 535.2 0 42 112 35 535.2 0 35 113 59 535.2 0 59 114 48 535.2 0 48 115 37 535.2 0 37 116 36 535.2 0 36 117 45 535.2 0 45 118 32 535.2 0 32 119 37 535.2 0 37 120 51 535.2 0 51 121 44 535.2 0 44 122 44 535.2 0 44 123 60 535.2 0 60 124 57 535.2 0 57 125 52 535.2 0 52 126 37 535.2 0 37 127 505 535.2 0 505 128 792 535.2 0 792 129 534 535.2 0 534 130 190 535.2 0 190 131 40 535.2 0 40 132 47 535.2 0 47 133 36 535.2 0 36 134 53 535.2 0 53 135 43 535.2 0 43 136 85 535.2 0 85 137 72 535.2 0 72 138 78 535.2 0 78 139 40 535.2 0 40 140 47 535.2 0 47 141 79 535.2 0 79 142 130 535.2 0 130 143 446 535.2 0 446 144 536 535.2 0 536 145 334 535.2 0 334 146 53 535.2 0 53 147 112 535.2 0 112 148 72 535.2 0 72 149 82 535.2 0 82 150 58 535.2 0 58 151 75 535.2 0 75 152 92 535.2 0 92 153 219 535.2 0 219 154 193 535.2 0 193 155 292 535.2 0 292 156 91 535.2 0 91 157 69 535.2 0 69 158 310 535.2 0 310 159 429 535.2 0 429 160 63 535.2 0 63 161 74 535.2 0 74 162 74 535.2 0 74 163 75 535.2 0 75 164 68 535.2 0 68 165 45 535.2 0 45 166 51 535.2 0 51 167 49 535.2 0 49 168 82 535.2 0 82 169 153 535.2 0 153 170 54 535.2 0 54 171 90 535.2 0 90 172 62 535.2 0 62 173 70 535.2 0 70 174 103 535.2 0 103 175 167 535.2 0 167 176 140 535.2 0 140 177 64 535.2 0 64 178 74 535.2 0 74 179 72 535.2 0 72 180 92 535.2 0 92 181 130 535.2 0 130 182 135 535.2 0 135 183 215 535.2 0 215 184 419 535.2 0 419 185 344 535.2 0 344 186 4655 535.2 0 4655 187 9084 535.2 0 9084 188 4536 535.2 0 4536 189 49 535.2 0 49 190 45 535.2 0 45 191 37 535.2 0 37 192 39 535.2 0 39 193 35 535.2 0 35 194 352 535.2 0 352 195 36 535.2 0 36 196 73 535.2 0 73 197 46 535.2 0 46 198 39 535.2 0 39 199 43 535.2 0 43 200 46 535.2 0 46 201 46 535.2 0 46 202 38 535.2 0 38 203 59 535.2 0 59 204 92 535.2 0 92 205 37 535.2 0 37 206 51 535.2 0 51 207 50 535.2 0 50 208 55 535.2 0 55 209 63 535.2 0 63 210 75 535.2 0 75 211 52 535.2 0 52 212 48 535.2 0 48 213 52 535.2 0 52 214 51 535.2 0 51 215 49 535.2 0 49 216 70 535.2 0 70 217 250 535.2 0 250 218 301 535.2 0 301 219 508 535.2 0 508 220 30 535.2 0 30 221 47 535.2 0 47 222 59 535.2 0 59 223 43 535.2 0 43 224 41 535.2 0 41 225 30 535.2 0 30 226 53 535.2 0 53 227 50 535.2 0 50 228 62 535.2 0 62 229 38 535.2 0 38 230 32 535.2 0 32 231 37 535.2 0 37 232 50 535.2 0 50 233 58 535.2 0 58 234 43 535.2 0 43 235 43 535.2 0 43 236 55 535.2 0 55 237 99 535.2 0 99 238 43 535.2 0 43 239 34 535.2 0 34 240 53 535.2 0 53 241 82 535.2 0 82 242 81 535.2 0 81 243 187 535.2 0 187 244 50 535.2 0 50 245 54 535.2 0 54 246 58 535.2 0 58 247 69 535.2 0 69 248 47 535.2 0 47 249 45 535.2 0 45 250 259 535.2 0 259 251 484 535.2 0 484 252 388 535.2 0 388 253 69 535.2 0 69 254 75 535.2 0 75 255 67 535.2 0 67 256 45 535.2 0 45 257 76 535.2 0 76 258 48 535.2 0 48 259 48 535.2 0 48 260 59 535.2 0 59 261 59 535.2 0 59 262 85 535.2 0 85 263 88 535.2 0 88 264 94 535.2 0 94 265 45 535.2 0 45 266 48 535.2 0 48 267 67 535.2 0 67 268 42 535.2 0 42 269 52 535.2 0 52 270 78 535.2 0 78 271 192 535.2 0 192 272 103 535.2 0 103 273 79 535.2 0 79 274 58 535.2 0 58 275 98 535.2 0 98 276 34 535.2 0 34 277 51 535.2 0 51 278 104 535.2 0 104 279 93 535.2 0 93 280 231 535.2 0 231 281 234 535.2 0 234 282 300 535.2 0 300 283 128 535.2 0 128 284 51 535.2 0 51 285 80 535.2 0 80 286 91 535.2 0 91 287 63 535.2 0 63 288 69 535.2 0 69 289 74 535.2 0 74 290 87 535.2 0 87 291 86 535.2 0 86 292 64 535.2 0 64 293 53 535.2 0 53 294 111 535.2 0 111 295 95 535.2 0 95 296 137 535.2 0 137 297 273 535.2 0 273 298 173 535.2 0 173 299 151 535.2 0 151 300 134 535.2 0 134 301 63 535.2 0 63 Finished in 10.13 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 238,154 Reads with adapters: 87,634 (36.8%) Reads that were too short: 58 (0.0%) Reads written (passing filters): 87,576 (36.8%) Total basepairs processed: 64,334,526 bp Total written (filtered): 24,079,433 bp (37.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 87634 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 18 0.0 3 0 0 18 16 524 0.0 3 0 515 8 1 17 86751 0.0 3 84933 1772 42 4 18 156 0.0 3 17 134 4 1 19 42 0.0 3 0 0 37 5 20 13 0.0 3 0 0 1 12 22 1 0.0 3 1 23 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 48 13 0.0 3 0 0 0 13 50 1 0.0 3 1 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 5 0.0 3 0 0 0 5 60 5 0.0 3 0 0 0 5 61 3 0.0 3 0 0 0 3 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 66 3 0.0 3 0 0 0 3 67 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 3 0.0 3 0 0 0 3 111 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 132 44 0.0 3 0 0 1 43 133 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 159 1 0.0 3 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 5 0.0 3 0 0 0 5 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.07 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 87,576 Reads with adapters: 477 (0.5%) Reads written (passing filters): 87,576 (100.0%) Total basepairs processed: 24,079,433 bp Total written (filtered): 24,077,945 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 477 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 62.1% C: 5.7% G: 29.8% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 432 1368.4 0 432 4 42 342.1 0 42 6 1 21.4 0 1 9 2 0.3 0 0 2