This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.16 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 81,590 (3.7%) Reads that were too short: 10,449 (0.5%) Reads written (passing filters): 71,141 (3.2%) Total basepairs processed: 659,880,795 bp Total written (filtered): 18,361,463 bp (2.8%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 81590 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 337 535.2 0 337 7 1439 535.2 0 1439 8 25893 535.2 0 25893 9 22309 535.2 0 22309 10 79 535.2 0 79 11 49 535.2 0 49 12 19 535.2 0 19 13 24 535.2 0 24 14 28 535.2 0 28 15 19 535.2 0 19 16 19 535.2 0 19 17 26 535.2 0 26 18 8 535.2 0 8 19 24 535.2 0 24 20 10 535.2 0 10 21 13 535.2 0 13 22 15 535.2 0 15 23 11 535.2 0 11 24 15 535.2 0 15 25 17 535.2 0 17 26 14 535.2 0 14 27 8 535.2 0 8 28 11 535.2 0 11 29 13 535.2 0 13 30 14 535.2 0 14 31 14 535.2 0 14 32 15 535.2 0 15 33 10 535.2 0 10 34 14 535.2 0 14 35 16 535.2 0 16 36 23 535.2 0 23 37 14 535.2 0 14 38 7 535.2 0 7 39 15 535.2 0 15 40 34 535.2 0 34 41 17 535.2 0 17 42 36 535.2 0 36 43 16 535.2 0 16 44 22 535.2 0 22 45 31 535.2 0 31 46 23 535.2 0 23 47 63 535.2 0 63 48 33 535.2 0 33 49 102 535.2 0 102 50 367 535.2 0 367 51 23 535.2 0 23 52 26 535.2 0 26 53 41 535.2 0 41 54 17 535.2 0 17 55 35 535.2 0 35 56 11 535.2 0 11 57 19 535.2 0 19 58 13 535.2 0 13 59 27 535.2 0 27 60 48 535.2 0 48 61 21 535.2 0 21 62 25 535.2 0 25 63 18 535.2 0 18 64 16 535.2 0 16 65 20 535.2 0 20 66 152 535.2 0 152 67 158 535.2 0 158 68 211 535.2 0 211 69 17 535.2 0 17 70 34 535.2 0 34 71 31 535.2 0 31 72 25 535.2 0 25 73 19 535.2 0 19 74 19 535.2 0 19 75 21 535.2 0 21 76 23 535.2 0 23 77 15 535.2 0 15 78 12 535.2 0 12 79 14 535.2 0 14 80 26 535.2 0 26 81 29 535.2 0 29 82 28 535.2 0 28 83 58 535.2 0 58 84 66 535.2 0 66 85 88 535.2 0 88 86 38 535.2 0 38 87 30 535.2 0 30 88 24 535.2 0 24 89 33 535.2 0 33 90 39 535.2 0 39 91 62 535.2 0 62 92 55 535.2 0 55 93 74 535.2 0 74 94 43 535.2 0 43 95 29 535.2 0 29 96 31 535.2 0 31 97 51 535.2 0 51 98 47 535.2 0 47 99 28 535.2 0 28 100 26 535.2 0 26 101 30 535.2 0 30 102 33 535.2 0 33 103 43 535.2 0 43 104 65 535.2 0 65 105 46 535.2 0 46 106 39 535.2 0 39 107 47 535.2 0 47 108 54 535.2 0 54 109 41 535.2 0 41 110 85 535.2 0 85 111 458 535.2 0 458 112 645 535.2 0 645 113 577 535.2 0 577 114 91 535.2 0 91 115 579 535.2 0 579 116 623 535.2 0 623 117 1840 535.2 0 1840 118 1715 535.2 0 1715 119 1949 535.2 0 1949 120 46 535.2 0 46 121 50 535.2 0 50 122 32 535.2 0 32 123 20 535.2 0 20 124 28 535.2 0 28 125 46 535.2 0 46 126 33 535.2 0 33 127 96 535.2 0 96 128 186 535.2 0 186 129 48 535.2 0 48 130 41 535.2 0 41 131 84 535.2 0 84 132 91 535.2 0 91 133 101 535.2 0 101 134 48 535.2 0 48 135 28 535.2 0 28 136 39 535.2 0 39 137 51 535.2 0 51 138 95 535.2 0 95 139 51 535.2 0 51 140 46 535.2 0 46 141 38 535.2 0 38 142 62 535.2 0 62 143 44 535.2 0 44 144 58 535.2 0 58 145 75 535.2 0 75 146 192 535.2 0 192 147 94 535.2 0 94 148 83 535.2 0 83 149 78 535.2 0 78 150 116 535.2 0 116 151 828 535.2 0 828 152 1510 535.2 0 1510 153 1378 535.2 0 1378 154 250 535.2 0 250 155 39 535.2 0 39 156 37 535.2 0 37 157 42 535.2 0 42 158 28 535.2 0 28 159 46 535.2 0 46 160 41 535.2 0 41 161 31 535.2 0 31 162 90 535.2 0 90 163 91 535.2 0 91 164 126 535.2 0 126 165 46 535.2 0 46 166 45 535.2 0 45 167 29 535.2 0 29 168 27 535.2 0 27 169 48 535.2 0 48 170 31 535.2 0 31 171 27 535.2 0 27 172 33 535.2 0 33 173 56 535.2 0 56 174 34 535.2 0 34 175 39 535.2 0 39 176 37 535.2 0 37 177 37 535.2 0 37 178 39 535.2 0 39 179 152 535.2 0 152 180 234 535.2 0 234 181 162 535.2 0 162 182 193 535.2 0 193 183 43 535.2 0 43 184 176 535.2 0 176 185 75 535.2 0 75 186 28 535.2 0 28 187 43 535.2 0 43 188 35 535.2 0 35 189 87 535.2 0 87 190 43 535.2 0 43 191 54 535.2 0 54 192 72 535.2 0 72 193 49 535.2 0 49 194 37 535.2 0 37 195 35 535.2 0 35 196 30 535.2 0 30 197 41 535.2 0 41 198 32 535.2 0 32 199 31 535.2 0 31 200 32 535.2 0 32 201 30 535.2 0 30 202 25 535.2 0 25 203 48 535.2 0 48 204 48 535.2 0 48 205 50 535.2 0 50 206 48 535.2 0 48 207 41 535.2 0 41 208 45 535.2 0 45 209 39 535.2 0 39 210 47 535.2 0 47 211 55 535.2 0 55 212 80 535.2 0 80 213 82 535.2 0 82 214 84 535.2 0 84 215 50 535.2 0 50 216 65 535.2 0 65 217 127 535.2 0 127 218 437 535.2 0 437 219 288 535.2 0 288 220 193 535.2 0 193 221 107 535.2 0 107 222 30 535.2 0 30 223 45 535.2 0 45 224 64 535.2 0 64 225 90 535.2 0 90 226 72 535.2 0 72 227 87 535.2 0 87 228 89 535.2 0 89 229 108 535.2 0 108 230 123 535.2 0 123 231 250 535.2 0 250 232 277 535.2 0 277 233 423 535.2 0 423 234 202 535.2 0 202 235 148 535.2 0 148 236 123 535.2 0 123 237 92 535.2 0 92 238 125 535.2 0 125 239 109 535.2 0 109 240 54 535.2 0 54 241 33 535.2 0 33 242 68 535.2 0 68 243 90 535.2 0 90 244 83 535.2 0 83 245 143 535.2 0 143 246 211 535.2 0 211 247 269 535.2 0 269 248 135 535.2 0 135 249 91 535.2 0 91 250 81 535.2 0 81 251 108 535.2 0 108 252 168 535.2 0 168 253 130 535.2 0 130 254 97 535.2 0 97 255 80 535.2 0 80 256 103 535.2 0 103 257 66 535.2 0 66 258 58 535.2 0 58 259 91 535.2 0 91 260 49 535.2 0 49 261 37 535.2 0 37 262 38 535.2 0 38 263 36 535.2 0 36 264 51 535.2 0 51 265 73 535.2 0 73 266 95 535.2 0 95 267 91 535.2 0 91 268 66 535.2 0 66 269 38 535.2 0 38 270 58 535.2 0 58 271 94 535.2 0 94 272 129 535.2 0 129 273 133 535.2 0 133 274 52 535.2 0 52 275 65 535.2 0 65 276 50 535.2 0 50 277 61 535.2 0 61 278 56 535.2 0 56 279 82 535.2 0 82 280 86 535.2 0 86 281 57 535.2 0 57 282 135 535.2 0 135 283 231 535.2 0 231 284 211 535.2 0 211 285 93 535.2 0 93 286 103 535.2 0 103 287 82 535.2 0 82 288 83 535.2 0 83 289 76 535.2 0 76 290 56 535.2 0 56 291 113 535.2 0 113 292 124 535.2 0 124 293 223 535.2 0 223 294 137 535.2 0 137 295 102 535.2 0 102 296 103 535.2 0 103 297 65 535.2 0 65 298 72 535.2 0 72 299 120 535.2 0 120 300 117 535.2 0 117 301 131 535.2 0 131 Finished in 3.57 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 71,141 Reads with adapters: 23,521 (33.1%) Reads that were too short: 41 (0.1%) Reads written (passing filters): 23,480 (33.0%) Total basepairs processed: 18,361,463 bp Total written (filtered): 6,385,535 bp (34.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 23521 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 4 18 21 0.0 3 0 0 11 6 4 19 345 0.0 3 0 268 36 29 12 20 22863 0.0 4 20878 1503 346 99 37 21 84 0.0 4 22 52 3 4 3 22 16 0.0 4 2 0 5 4 5 23 123 0.0 4 5 0 0 71 47 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 0 0 1 28 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 1 51 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 91 3 0.0 4 0 0 0 0 3 92 3 0.0 4 0 0 0 0 3 95 1 0.0 4 1 102 1 0.0 4 0 0 0 1 121 1 0.0 4 0 0 0 1 127 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 132 2 0.0 4 0 0 0 0 2 134 3 0.0 4 0 0 0 0 3 137 1 0.0 4 0 0 1 138 3 0.0 4 0 0 0 0 3 139 1 0.0 4 0 0 0 0 1 140 3 0.0 4 0 0 0 0 3 147 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 156 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 184 2 0.0 4 0 0 0 0 2 186 3 0.0 4 0 0 0 0 3 194 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 1 221 1 0.0 4 0 0 1 228 3 0.0 4 0 0 0 0 3 230 1 0.0 4 0 0 0 0 1 233 3 0.0 4 0 0 0 0 3 254 1 0.0 4 0 0 0 0 1 257 3 0.0 4 0 0 0 0 3 259 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 1 284 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.87 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 23,480 Reads with adapters: 5,556 (23.7%) Reads written (passing filters): 23,480 (100.0%) Total basepairs processed: 6,385,535 bp Total written (filtered): 6,328,414 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5556 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 9.6% G: 84.9% T: 2.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 16 366.9 0 16 4 9 91.7 0 9 5 2 22.9 0 2 6 7 5.7 0 7 7 2 1.4 0 2 8 12 0.4 0 12 9 797 0.1 0 82 715 10 4090 0.0 1 327 3763 11 586 0.0 1 37 549 12 10 0.0 1 0 10 13 13 0.0 1 0 13 15 1 0.0 1 0 1 145 2 0.0 1 2 150 2 0.0 1 1 1 171 1 0.0 1 1 208 3 0.0 1 0 3 221 1 0.0 1 0 1 223 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.