This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.80 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 71,024 (3.2%) Reads that were too short: 4,120 (0.2%) Reads written (passing filters): 66,904 (3.1%) Total basepairs processed: 659,880,795 bp Total written (filtered): 18,028,825 bp (2.7%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 71024 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 293 535.2 0 293 7 985 535.2 0 985 8 25042 535.2 0 25042 9 26826 535.2 0 26826 10 35 535.2 0 35 11 17 535.2 0 17 12 26 535.2 0 26 13 16 535.2 0 16 14 18 535.2 0 18 15 16 535.2 0 16 16 29 535.2 0 29 17 23 535.2 0 23 18 12 535.2 0 12 19 29 535.2 0 29 20 12 535.2 0 12 21 14 535.2 0 14 22 27 535.2 0 27 23 15 535.2 0 15 24 9 535.2 0 9 25 14 535.2 0 14 26 15 535.2 0 15 27 10 535.2 0 10 28 15 535.2 0 15 29 11 535.2 0 11 30 25 535.2 0 25 31 16 535.2 0 16 32 12 535.2 0 12 33 14 535.2 0 14 34 29 535.2 0 29 35 18 535.2 0 18 36 24 535.2 0 24 37 17 535.2 0 17 38 21 535.2 0 21 39 17 535.2 0 17 40 21 535.2 0 21 41 16 535.2 0 16 42 16 535.2 0 16 43 8 535.2 0 8 44 24 535.2 0 24 45 36 535.2 0 36 46 26 535.2 0 26 47 40 535.2 0 40 48 10 535.2 0 10 49 29 535.2 0 29 50 12 535.2 0 12 51 14 535.2 0 14 52 22 535.2 0 22 53 32 535.2 0 32 54 14 535.2 0 14 55 46 535.2 0 46 56 21 535.2 0 21 57 12 535.2 0 12 58 24 535.2 0 24 59 17 535.2 0 17 60 21 535.2 0 21 61 17 535.2 0 17 62 28 535.2 0 28 63 19 535.2 0 19 64 11 535.2 0 11 65 13 535.2 0 13 66 62 535.2 0 62 67 52 535.2 0 52 68 48 535.2 0 48 69 16 535.2 0 16 70 21 535.2 0 21 71 23 535.2 0 23 72 28 535.2 0 28 73 11 535.2 0 11 74 15 535.2 0 15 75 20 535.2 0 20 76 23 535.2 0 23 77 16 535.2 0 16 78 13 535.2 0 13 79 29 535.2 0 29 80 22 535.2 0 22 81 17 535.2 0 17 82 29 535.2 0 29 83 22 535.2 0 22 84 23 535.2 0 23 85 22 535.2 0 22 86 23 535.2 0 23 87 21 535.2 0 21 88 41 535.2 0 41 89 25 535.2 0 25 90 12 535.2 0 12 91 55 535.2 0 55 92 40 535.2 0 40 93 45 535.2 0 45 94 32 535.2 0 32 95 27 535.2 0 27 96 23 535.2 0 23 97 43 535.2 0 43 98 54 535.2 0 54 99 25 535.2 0 25 100 26 535.2 0 26 101 27 535.2 0 27 102 36 535.2 0 36 103 31 535.2 0 31 104 62 535.2 0 62 105 41 535.2 0 41 106 43 535.2 0 43 107 48 535.2 0 48 108 41 535.2 0 41 109 47 535.2 0 47 110 79 535.2 0 79 111 460 535.2 0 460 112 701 535.2 0 701 113 567 535.2 0 567 114 41 535.2 0 41 115 169 535.2 0 169 116 164 535.2 0 164 117 1127 535.2 0 1127 118 1741 535.2 0 1741 119 2064 535.2 0 2064 120 30 535.2 0 30 121 20 535.2 0 20 122 15 535.2 0 15 123 19 535.2 0 19 124 29 535.2 0 29 125 25 535.2 0 25 126 29 535.2 0 29 127 47 535.2 0 47 128 150 535.2 0 150 129 23 535.2 0 23 130 21 535.2 0 21 131 34 535.2 0 34 132 45 535.2 0 45 133 66 535.2 0 66 134 26 535.2 0 26 135 27 535.2 0 27 136 21 535.2 0 21 137 24 535.2 0 24 138 58 535.2 0 58 139 35 535.2 0 35 140 25 535.2 0 25 141 23 535.2 0 23 142 20 535.2 0 20 143 18 535.2 0 18 144 26 535.2 0 26 145 21 535.2 0 21 146 44 535.2 0 44 147 39 535.2 0 39 148 36 535.2 0 36 149 33 535.2 0 33 150 28 535.2 0 28 151 294 535.2 0 294 152 467 535.2 0 467 153 415 535.2 0 415 154 72 535.2 0 72 155 33 535.2 0 33 156 20 535.2 0 20 157 31 535.2 0 31 158 13 535.2 0 13 159 29 535.2 0 29 160 29 535.2 0 29 161 25 535.2 0 25 162 21 535.2 0 21 163 31 535.2 0 31 164 33 535.2 0 33 165 13 535.2 0 13 166 14 535.2 0 14 167 12 535.2 0 12 168 15 535.2 0 15 169 41 535.2 0 41 170 9 535.2 0 9 171 10 535.2 0 10 172 15 535.2 0 15 173 25 535.2 0 25 174 19 535.2 0 19 175 17 535.2 0 17 176 29 535.2 0 29 177 21 535.2 0 21 178 19 535.2 0 19 179 78 535.2 0 78 180 245 535.2 0 245 181 149 535.2 0 149 182 210 535.2 0 210 183 29 535.2 0 29 184 183 535.2 0 183 185 68 535.2 0 68 186 9 535.2 0 9 187 19 535.2 0 19 188 10 535.2 0 10 189 60 535.2 0 60 190 35 535.2 0 35 191 25 535.2 0 25 192 48 535.2 0 48 193 17 535.2 0 17 194 21 535.2 0 21 195 8 535.2 0 8 196 18 535.2 0 18 197 17 535.2 0 17 198 17 535.2 0 17 199 17 535.2 0 17 200 22 535.2 0 22 201 26 535.2 0 26 202 21 535.2 0 21 203 23 535.2 0 23 204 28 535.2 0 28 205 16 535.2 0 16 206 22 535.2 0 22 207 19 535.2 0 19 208 22 535.2 0 22 209 16 535.2 0 16 210 55 535.2 0 55 211 34 535.2 0 34 212 65 535.2 0 65 213 34 535.2 0 34 214 22 535.2 0 22 215 31 535.2 0 31 216 42 535.2 0 42 217 32 535.2 0 32 218 538 535.2 0 538 219 270 535.2 0 270 220 203 535.2 0 203 221 106 535.2 0 106 222 19 535.2 0 19 223 35 535.2 0 35 224 19 535.2 0 19 225 33 535.2 0 33 226 17 535.2 0 17 227 30 535.2 0 30 228 37 535.2 0 37 229 47 535.2 0 47 230 32 535.2 0 32 231 52 535.2 0 52 232 53 535.2 0 53 233 73 535.2 0 73 234 57 535.2 0 57 235 36 535.2 0 36 236 40 535.2 0 40 237 46 535.2 0 46 238 28 535.2 0 28 239 23 535.2 0 23 240 20 535.2 0 20 241 17 535.2 0 17 242 24 535.2 0 24 243 22 535.2 0 22 244 21 535.2 0 21 245 23 535.2 0 23 246 35 535.2 0 35 247 59 535.2 0 59 248 37 535.2 0 37 249 19 535.2 0 19 250 16 535.2 0 16 251 39 535.2 0 39 252 41 535.2 0 41 253 24 535.2 0 24 254 39 535.2 0 39 255 28 535.2 0 28 256 27 535.2 0 27 257 27 535.2 0 27 258 18 535.2 0 18 259 25 535.2 0 25 260 19 535.2 0 19 261 11 535.2 0 11 262 14 535.2 0 14 263 18 535.2 0 18 264 33 535.2 0 33 265 25 535.2 0 25 266 29 535.2 0 29 267 28 535.2 0 28 268 13 535.2 0 13 269 27 535.2 0 27 270 28 535.2 0 28 271 29 535.2 0 29 272 27 535.2 0 27 273 35 535.2 0 35 274 38 535.2 0 38 275 22 535.2 0 22 276 40 535.2 0 40 277 17 535.2 0 17 278 34 535.2 0 34 279 33 535.2 0 33 280 41 535.2 0 41 281 24 535.2 0 24 282 24 535.2 0 24 283 51 535.2 0 51 284 38 535.2 0 38 285 29 535.2 0 29 286 26 535.2 0 26 287 30 535.2 0 30 288 33 535.2 0 33 289 28 535.2 0 28 290 27 535.2 0 27 291 40 535.2 0 40 292 46 535.2 0 46 293 56 535.2 0 56 294 27 535.2 0 27 295 37 535.2 0 37 296 41 535.2 0 41 297 33 535.2 0 33 298 23 535.2 0 23 299 34 535.2 0 34 300 33 535.2 0 33 301 42 535.2 0 42 Finished in 3.20 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 66,904 Reads with adapters: 25,012 (37.4%) Reads that were too short: 30 (0.0%) Reads written (passing filters): 24,982 (37.3%) Total basepairs processed: 18,028,825 bp Total written (filtered): 6,885,285 bp (38.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 25012 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 11 0.0 3 0 0 10 1 16 221 0.0 3 0 216 5 17 24593 0.0 3 24135 443 13 2 18 65 0.0 3 6 56 2 1 19 8 0.0 3 0 0 6 2 20 14 0.0 3 0 0 0 14 21 1 0.0 3 0 0 1 22 1 0.0 3 0 0 0 1 40 12 0.0 3 0 0 0 12 43 2 0.0 3 0 0 0 2 54 19 0.0 3 0 0 0 19 55 1 0.0 3 0 0 0 1 56 3 0.0 3 0 0 0 3 57 3 0.0 3 0 0 0 3 58 12 0.0 3 0 0 0 12 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 66 4 0.0 3 0 0 0 4 86 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 166 3 0.0 3 0 0 0 3 168 1 0.0 3 0 0 0 1 169 7 0.0 3 0 0 0 7 170 2 0.0 3 0 0 0 2 173 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 208 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.88 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 24,982 Reads with adapters: 38 (0.2%) Reads written (passing filters): 24,982 (100.0%) Total basepairs processed: 6,885,285 bp Total written (filtered): 6,885,166 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 38 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 73.7% C: 10.5% G: 7.9% T: 7.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 34 390.3 0 34 4 3 97.6 0 3 5 1 24.4 0 1