This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.72 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 405,436 (18.5%) Reads that were too short: 47,779 (2.2%) Reads written (passing filters): 357,657 (16.3%) Total basepairs processed: 659,880,795 bp Total written (filtered): 89,729,926 bp (13.6%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 405436 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 308 535.2 0 308 7 4128 535.2 0 4128 8 3409 535.2 0 3409 9 212 535.2 0 212 10 275 535.2 0 275 11 172 535.2 0 172 12 205 535.2 0 205 13 321 535.2 0 321 14 415 535.2 0 415 15 402 535.2 0 402 16 372 535.2 0 372 17 527 535.2 0 527 18 676 535.2 0 676 19 1072 535.2 0 1072 20 16104 535.2 0 16104 21 25812 535.2 0 25812 22 28950 535.2 0 28950 23 345 535.2 0 345 24 265 535.2 0 265 25 408 535.2 0 408 26 171 535.2 0 171 27 144 535.2 0 144 28 118 535.2 0 118 29 176 535.2 0 176 30 113 535.2 0 113 31 210 535.2 0 210 32 306 535.2 0 306 33 350 535.2 0 350 34 312 535.2 0 312 35 652 535.2 0 652 36 1503 535.2 0 1503 37 426 535.2 0 426 38 482 535.2 0 482 39 1054 535.2 0 1054 40 545 535.2 0 545 41 623 535.2 0 623 42 1023 535.2 0 1023 43 1083 535.2 0 1083 44 1168 535.2 0 1168 45 1110 535.2 0 1110 46 1560 535.2 0 1560 47 1952 535.2 0 1952 48 3414 535.2 0 3414 49 54243 535.2 0 54243 50 88409 535.2 0 88409 51 84964 535.2 0 84964 52 369 535.2 0 369 53 68 535.2 0 68 54 62 535.2 0 62 55 45 535.2 0 45 56 68 535.2 0 68 57 83 535.2 0 83 58 78 535.2 0 78 59 52 535.2 0 52 60 78 535.2 0 78 61 104 535.2 0 104 62 156 535.2 0 156 63 743 535.2 0 743 64 974 535.2 0 974 65 1223 535.2 0 1223 66 38 535.2 0 38 67 49 535.2 0 49 68 53 535.2 0 53 69 31 535.2 0 31 70 58 535.2 0 58 71 57 535.2 0 57 72 61 535.2 0 61 73 66 535.2 0 66 74 84 535.2 0 84 75 63 535.2 0 63 76 59 535.2 0 59 77 44 535.2 0 44 78 35 535.2 0 35 79 52 535.2 0 52 80 38 535.2 0 38 81 54 535.2 0 54 82 49 535.2 0 49 83 67 535.2 0 67 84 62 535.2 0 62 85 41 535.2 0 41 86 63 535.2 0 63 87 63 535.2 0 63 88 89 535.2 0 89 89 72 535.2 0 72 90 91 535.2 0 91 91 45 535.2 0 45 92 60 535.2 0 60 93 41 535.2 0 41 94 59 535.2 0 59 95 47 535.2 0 47 96 40 535.2 0 40 97 61 535.2 0 61 98 59 535.2 0 59 99 35 535.2 0 35 100 32 535.2 0 32 101 50 535.2 0 50 102 38 535.2 0 38 103 33 535.2 0 33 104 34 535.2 0 34 105 55 535.2 0 55 106 74 535.2 0 74 107 56 535.2 0 56 108 37 535.2 0 37 109 45 535.2 0 45 110 45 535.2 0 45 111 47 535.2 0 47 112 50 535.2 0 50 113 49 535.2 0 49 114 45 535.2 0 45 115 67 535.2 0 67 116 64 535.2 0 64 117 324 535.2 0 324 118 426 535.2 0 426 119 632 535.2 0 632 120 133 535.2 0 133 121 85 535.2 0 85 122 45 535.2 0 45 123 68 535.2 0 68 124 103 535.2 0 103 125 103 535.2 0 103 126 157 535.2 0 157 127 97 535.2 0 97 128 79 535.2 0 79 129 48 535.2 0 48 130 51 535.2 0 51 131 74 535.2 0 74 132 56 535.2 0 56 133 66 535.2 0 66 134 76 535.2 0 76 135 61 535.2 0 61 136 43 535.2 0 43 137 40 535.2 0 40 138 75 535.2 0 75 139 82 535.2 0 82 140 101 535.2 0 101 141 79 535.2 0 79 142 88 535.2 0 88 143 73 535.2 0 73 144 127 535.2 0 127 145 98 535.2 0 98 146 102 535.2 0 102 147 70 535.2 0 70 148 69 535.2 0 69 149 76 535.2 0 76 150 59 535.2 0 59 151 64 535.2 0 64 152 99 535.2 0 99 153 119 535.2 0 119 154 105 535.2 0 105 155 75 535.2 0 75 156 55 535.2 0 55 157 90 535.2 0 90 158 145 535.2 0 145 159 174 535.2 0 174 160 71 535.2 0 71 161 56 535.2 0 56 162 54 535.2 0 54 163 50 535.2 0 50 164 62 535.2 0 62 165 82 535.2 0 82 166 51 535.2 0 51 167 71 535.2 0 71 168 76 535.2 0 76 169 62 535.2 0 62 170 65 535.2 0 65 171 62 535.2 0 62 172 98 535.2 0 98 173 91 535.2 0 91 174 79 535.2 0 79 175 75 535.2 0 75 176 104 535.2 0 104 177 152 535.2 0 152 178 244 535.2 0 244 179 210 535.2 0 210 180 201 535.2 0 201 181 166 535.2 0 166 182 1616 535.2 0 1616 183 929 535.2 0 929 184 1106 535.2 0 1106 185 97 535.2 0 97 186 135 535.2 0 135 187 89 535.2 0 89 188 140 535.2 0 140 189 196 535.2 0 196 190 244 535.2 0 244 191 187 535.2 0 187 192 178 535.2 0 178 193 1333 535.2 0 1333 194 1478 535.2 0 1478 195 1844 535.2 0 1844 196 1094 535.2 0 1094 197 1908 535.2 0 1908 198 475 535.2 0 475 199 91 535.2 0 91 200 126 535.2 0 126 201 118 535.2 0 118 202 84 535.2 0 84 203 97 535.2 0 97 204 169 535.2 0 169 205 98 535.2 0 98 206 140 535.2 0 140 207 166 535.2 0 166 208 133 535.2 0 133 209 93 535.2 0 93 210 87 535.2 0 87 211 100 535.2 0 100 212 104 535.2 0 104 213 467 535.2 0 467 214 1059 535.2 0 1059 215 1463 535.2 0 1463 216 1120 535.2 0 1120 217 273 535.2 0 273 218 329 535.2 0 329 219 608 535.2 0 608 220 723 535.2 0 723 221 789 535.2 0 789 222 372 535.2 0 372 223 251 535.2 0 251 224 101 535.2 0 101 225 100 535.2 0 100 226 140 535.2 0 140 227 153 535.2 0 153 228 92 535.2 0 92 229 128 535.2 0 128 230 90 535.2 0 90 231 142 535.2 0 142 232 195 535.2 0 195 233 201 535.2 0 201 234 167 535.2 0 167 235 144 535.2 0 144 236 420 535.2 0 420 237 273 535.2 0 273 238 518 535.2 0 518 239 929 535.2 0 929 240 2183 535.2 0 2183 241 1423 535.2 0 1423 242 405 535.2 0 405 243 58 535.2 0 58 244 77 535.2 0 77 245 118 535.2 0 118 246 166 535.2 0 166 247 188 535.2 0 188 248 153 535.2 0 153 249 190 535.2 0 190 250 559 535.2 0 559 251 1239 535.2 0 1239 252 1339 535.2 0 1339 253 531 535.2 0 531 254 106 535.2 0 106 255 127 535.2 0 127 256 163 535.2 0 163 257 216 535.2 0 216 258 234 535.2 0 234 259 1058 535.2 0 1058 260 2144 535.2 0 2144 261 1665 535.2 0 1665 262 225 535.2 0 225 263 856 535.2 0 856 264 1488 535.2 0 1488 265 1874 535.2 0 1874 266 456 535.2 0 456 267 562 535.2 0 562 268 279 535.2 0 279 269 266 535.2 0 266 270 295 535.2 0 295 271 195 535.2 0 195 272 147 535.2 0 147 273 88 535.2 0 88 274 120 535.2 0 120 275 169 535.2 0 169 276 169 535.2 0 169 277 149 535.2 0 149 278 151 535.2 0 151 279 93 535.2 0 93 280 106 535.2 0 106 281 172 535.2 0 172 282 163 535.2 0 163 283 270 535.2 0 270 284 291 535.2 0 291 285 294 535.2 0 294 286 244 535.2 0 244 287 243 535.2 0 243 288 2207 535.2 0 2207 289 3299 535.2 0 3299 290 3021 535.2 0 3021 291 418 535.2 0 418 292 166 535.2 0 166 293 322 535.2 0 322 294 255 535.2 0 255 295 275 535.2 0 275 296 154 535.2 0 154 297 163 535.2 0 163 298 261 535.2 0 261 299 325 535.2 0 325 300 460 535.2 0 460 301 228 535.2 0 228 Finished in 16.59 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 357,657 Reads with adapters: 7,486 (2.1%) Reads that were too short: 260 (0.1%) Reads written (passing filters): 7,226 (2.0%) Total basepairs processed: 89,729,926 bp Total written (filtered): 1,568,339 bp (1.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7486 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 6 0.0 3 0 0 4 2 19 47 0.0 3 0 38 4 4 1 20 3358 0.0 4 3063 233 37 19 6 21 4 0.0 4 1 3 23 13 0.0 4 1 0 0 7 5 25 1 0.0 4 0 1 39 1 0.0 4 0 0 0 0 1 49 4 0.0 4 0 0 0 0 4 50 9 0.0 4 0 0 0 0 9 64 1 0.0 4 0 0 0 1 68 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 1 76 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 78 2 0.0 4 0 0 0 0 2 79 1 0.0 4 0 0 0 0 1 81 2 0.0 4 0 0 0 0 2 83 2 0.0 4 0 0 0 0 2 84 4 0.0 4 0 0 0 0 4 85 3215 0.0 4 0 0 0 0 3215 86 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 88 11 0.0 4 0 0 0 1 10 89 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 1 1 97 6 0.0 4 0 0 0 0 6 98 4 0.0 4 0 0 0 0 4 103 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 110 4 0.0 4 0 0 0 0 4 111 5 0.0 4 0 0 0 0 5 112 2 0.0 4 0 0 0 0 2 113 1 0.0 4 0 0 0 0 1 114 489 0.0 4 0 0 0 0 489 115 2 0.0 4 1 0 0 0 1 116 1 0.0 4 0 0 0 0 1 117 3 0.0 4 0 0 0 1 2 120 1 0.0 4 0 0 0 0 1 121 2 0.0 4 0 0 0 1 1 124 3 0.0 4 0 0 0 0 3 127 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 1 132 2 0.0 4 0 1 0 0 1 139 2 0.0 4 0 0 0 0 2 140 8 0.0 4 0 0 0 0 8 142 1 0.0 4 0 0 0 0 1 146 2 0.0 4 0 0 0 0 2 151 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 1 159 1 0.0 4 0 0 0 0 1 161 2 0.0 4 0 0 0 0 2 162 1 0.0 4 0 0 0 0 1 163 5 0.0 4 0 0 0 0 5 164 1 0.0 4 0 0 0 0 1 165 2 0.0 4 0 0 0 0 2 166 4 0.0 4 0 0 0 0 4 167 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 171 2 0.0 4 0 0 0 0 2 172 8 0.0 4 0 0 0 0 8 173 11 0.0 4 0 0 0 2 9 175 2 0.0 4 0 0 0 0 2 176 1 0.0 4 0 0 0 0 1 177 2 0.0 4 0 1 0 0 1 181 1 0.0 4 0 0 0 0 1 183 5 0.0 4 0 0 0 0 5 184 2 0.0 4 0 0 0 0 2 186 20 0.0 4 0 0 0 1 19 187 10 0.0 4 0 0 0 2 8 188 5 0.0 4 0 0 0 1 4 189 1 0.0 4 0 0 0 0 1 191 6 0.0 4 0 0 0 1 5 192 5 0.0 4 0 0 0 0 5 193 5 0.0 4 0 0 0 0 5 194 3 0.0 4 0 0 0 0 3 195 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 202 2 0.0 4 0 0 0 0 2 203 2 0.0 4 0 0 0 0 2 207 1 0.0 4 0 0 0 0 1 209 2 0.0 4 0 0 0 2 210 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 215 9 0.0 4 0 0 0 0 9 216 7 0.0 4 0 0 0 0 7 217 2 0.0 4 0 0 0 1 1 218 1 0.0 4 0 0 0 0 1 220 4 0.0 4 0 0 0 0 4 221 7 0.0 4 0 0 0 0 7 222 6 0.0 4 0 0 0 0 6 223 7 0.0 4 0 0 0 0 7 224 2 0.0 4 0 0 0 0 2 228 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 233 3 0.0 4 0 0 0 1 2 234 2 0.0 4 0 0 0 0 2 238 1 0.0 4 0 0 0 0 1 240 7 0.0 4 0 0 0 1 6 241 3 0.0 4 0 0 0 0 3 242 3 0.0 4 0 0 0 0 3 243 5 0.0 4 0 0 0 1 4 244 2 0.0 4 0 0 0 0 2 246 18 0.0 4 0 0 0 0 18 247 2 0.0 4 0 0 0 0 2 248 3 0.0 4 0 0 0 1 2 250 3 0.0 4 0 0 0 0 3 251 7 0.0 4 0 0 0 1 6 252 2 0.0 4 0 0 0 0 2 254 1 0.0 4 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 269 5 0.0 4 0 0 0 0 5 272 1 0.0 4 0 0 0 0 1 275 2 0.0 4 0 0 0 0 2 276 2 0.0 4 0 0 0 0 2 277 1 0.0 4 0 0 0 0 1 278 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 7,226 Reads with adapters: 819 (11.3%) Reads written (passing filters): 7,226 (100.0%) Total basepairs processed: 1,568,339 bp Total written (filtered): 1,559,481 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 819 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.1% C: 9.5% G: 79.6% T: 2.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 28 112.9 0 28 4 21 28.2 0 21 5 1 7.1 0 1 9 6 0.0 0 0 6 10 54 0.0 1 4 50 11 702 0.0 1 26 676 12 5 0.0 1 0 5 139 1 0.0 1 1 170 1 0.0 1 1