This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.97 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 319,358 (14.6%) Reads that were too short: 16,564 (0.8%) Reads written (passing filters): 302,794 (13.8%) Total basepairs processed: 659,880,795 bp Total written (filtered): 75,758,094 bp (11.5%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 319358 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 274 535.2 0 274 7 3984 535.2 0 3984 8 4122 535.2 0 4122 9 201 535.2 0 201 10 237 535.2 0 237 11 124 535.2 0 124 12 171 535.2 0 171 13 280 535.2 0 280 14 340 535.2 0 340 15 278 535.2 0 278 16 252 535.2 0 252 17 436 535.2 0 436 18 449 535.2 0 449 19 776 535.2 0 776 20 14031 535.2 0 14031 21 23281 535.2 0 23281 22 24998 535.2 0 24998 23 290 535.2 0 290 24 188 535.2 0 188 25 312 535.2 0 312 26 171 535.2 0 171 27 160 535.2 0 160 28 120 535.2 0 120 29 173 535.2 0 173 30 92 535.2 0 92 31 153 535.2 0 153 32 231 535.2 0 231 33 275 535.2 0 275 34 318 535.2 0 318 35 597 535.2 0 597 36 1359 535.2 0 1359 37 361 535.2 0 361 38 413 535.2 0 413 39 875 535.2 0 875 40 401 535.2 0 401 41 490 535.2 0 490 42 833 535.2 0 833 43 924 535.2 0 924 44 930 535.2 0 930 45 834 535.2 0 834 46 1303 535.2 0 1303 47 1352 535.2 0 1352 48 2243 535.2 0 2243 49 43150 535.2 0 43150 50 75549 535.2 0 75549 51 71047 535.2 0 71047 52 258 535.2 0 258 53 60 535.2 0 60 54 60 535.2 0 60 55 37 535.2 0 37 56 56 535.2 0 56 57 64 535.2 0 64 58 68 535.2 0 68 59 67 535.2 0 67 60 74 535.2 0 74 61 78 535.2 0 78 62 80 535.2 0 80 63 155 535.2 0 155 64 172 535.2 0 172 65 265 535.2 0 265 66 53 535.2 0 53 67 50 535.2 0 50 68 49 535.2 0 49 69 42 535.2 0 42 70 53 535.2 0 53 71 53 535.2 0 53 72 48 535.2 0 48 73 59 535.2 0 59 74 53 535.2 0 53 75 51 535.2 0 51 76 45 535.2 0 45 77 42 535.2 0 42 78 63 535.2 0 63 79 69 535.2 0 69 80 50 535.2 0 50 81 53 535.2 0 53 82 58 535.2 0 58 83 53 535.2 0 53 84 60 535.2 0 60 85 46 535.2 0 46 86 70 535.2 0 70 87 53 535.2 0 53 88 59 535.2 0 59 89 56 535.2 0 56 90 71 535.2 0 71 91 50 535.2 0 50 92 39 535.2 0 39 93 75 535.2 0 75 94 36 535.2 0 36 95 45 535.2 0 45 96 38 535.2 0 38 97 35 535.2 0 35 98 89 535.2 0 89 99 37 535.2 0 37 100 38 535.2 0 38 101 44 535.2 0 44 102 45 535.2 0 45 103 33 535.2 0 33 104 44 535.2 0 44 105 35 535.2 0 35 106 76 535.2 0 76 107 44 535.2 0 44 108 35 535.2 0 35 109 47 535.2 0 47 110 48 535.2 0 48 111 54 535.2 0 54 112 64 535.2 0 64 113 70 535.2 0 70 114 39 535.2 0 39 115 71 535.2 0 71 116 50 535.2 0 50 117 105 535.2 0 105 118 95 535.2 0 95 119 155 535.2 0 155 120 60 535.2 0 60 121 45 535.2 0 45 122 55 535.2 0 55 123 52 535.2 0 52 124 82 535.2 0 82 125 70 535.2 0 70 126 132 535.2 0 132 127 64 535.2 0 64 128 65 535.2 0 65 129 52 535.2 0 52 130 75 535.2 0 75 131 90 535.2 0 90 132 58 535.2 0 58 133 43 535.2 0 43 134 56 535.2 0 56 135 56 535.2 0 56 136 53 535.2 0 53 137 52 535.2 0 52 138 79 535.2 0 79 139 62 535.2 0 62 140 51 535.2 0 51 141 78 535.2 0 78 142 73 535.2 0 73 143 71 535.2 0 71 144 80 535.2 0 80 145 71 535.2 0 71 146 70 535.2 0 70 147 77 535.2 0 77 148 73 535.2 0 73 149 81 535.2 0 81 150 61 535.2 0 61 151 62 535.2 0 62 152 69 535.2 0 69 153 72 535.2 0 72 154 69 535.2 0 69 155 65 535.2 0 65 156 77 535.2 0 77 157 63 535.2 0 63 158 65 535.2 0 65 159 61 535.2 0 61 160 80 535.2 0 80 161 63 535.2 0 63 162 68 535.2 0 68 163 39 535.2 0 39 164 60 535.2 0 60 165 74 535.2 0 74 166 66 535.2 0 66 167 61 535.2 0 61 168 59 535.2 0 59 169 60 535.2 0 60 170 81 535.2 0 81 171 77 535.2 0 77 172 55 535.2 0 55 173 77 535.2 0 77 174 65 535.2 0 65 175 100 535.2 0 100 176 97 535.2 0 97 177 80 535.2 0 80 178 101 535.2 0 101 179 90 535.2 0 90 180 171 535.2 0 171 181 163 535.2 0 163 182 2292 535.2 0 2292 183 1279 535.2 0 1279 184 1641 535.2 0 1641 185 123 535.2 0 123 186 83 535.2 0 83 187 69 535.2 0 69 188 129 535.2 0 129 189 107 535.2 0 107 190 161 535.2 0 161 191 126 535.2 0 126 192 139 535.2 0 139 193 1193 535.2 0 1193 194 1286 535.2 0 1286 195 1867 535.2 0 1867 196 1093 535.2 0 1093 197 2139 535.2 0 2139 198 511 535.2 0 511 199 60 535.2 0 60 200 92 535.2 0 92 201 60 535.2 0 60 202 70 535.2 0 70 203 79 535.2 0 79 204 114 535.2 0 114 205 71 535.2 0 71 206 97 535.2 0 97 207 129 535.2 0 129 208 105 535.2 0 105 209 47 535.2 0 47 210 61 535.2 0 61 211 77 535.2 0 77 212 58 535.2 0 58 213 152 535.2 0 152 214 277 535.2 0 277 215 421 535.2 0 421 216 364 535.2 0 364 217 121 535.2 0 121 218 126 535.2 0 126 219 174 535.2 0 174 220 177 535.2 0 177 221 164 535.2 0 164 222 169 535.2 0 169 223 109 535.2 0 109 224 70 535.2 0 70 225 70 535.2 0 70 226 52 535.2 0 52 227 85 535.2 0 85 228 82 535.2 0 82 229 66 535.2 0 66 230 85 535.2 0 85 231 68 535.2 0 68 232 90 535.2 0 90 233 80 535.2 0 80 234 108 535.2 0 108 235 65 535.2 0 65 236 206 535.2 0 206 237 93 535.2 0 93 238 118 535.2 0 118 239 242 535.2 0 242 240 648 535.2 0 648 241 407 535.2 0 407 242 130 535.2 0 130 243 55 535.2 0 55 244 57 535.2 0 57 245 66 535.2 0 66 246 64 535.2 0 64 247 86 535.2 0 86 248 77 535.2 0 77 249 111 535.2 0 111 250 243 535.2 0 243 251 445 535.2 0 445 252 570 535.2 0 570 253 225 535.2 0 225 254 56 535.2 0 56 255 98 535.2 0 98 256 79 535.2 0 79 257 98 535.2 0 98 258 110 535.2 0 110 259 228 535.2 0 228 260 385 535.2 0 385 261 390 535.2 0 390 262 87 535.2 0 87 263 260 535.2 0 260 264 410 535.2 0 410 265 457 535.2 0 457 266 142 535.2 0 142 267 183 535.2 0 183 268 100 535.2 0 100 269 113 535.2 0 113 270 106 535.2 0 106 271 83 535.2 0 83 272 103 535.2 0 103 273 69 535.2 0 69 274 72 535.2 0 72 275 80 535.2 0 80 276 79 535.2 0 79 277 73 535.2 0 73 278 81 535.2 0 81 279 67 535.2 0 67 280 68 535.2 0 68 281 75 535.2 0 75 282 85 535.2 0 85 283 98 535.2 0 98 284 114 535.2 0 114 285 118 535.2 0 118 286 82 535.2 0 82 287 96 535.2 0 96 288 696 535.2 0 696 289 999 535.2 0 999 290 924 535.2 0 924 291 156 535.2 0 156 292 57 535.2 0 57 293 103 535.2 0 103 294 99 535.2 0 99 295 80 535.2 0 80 296 96 535.2 0 96 297 64 535.2 0 64 298 86 535.2 0 86 299 98 535.2 0 98 300 265 535.2 0 265 301 170 535.2 0 170 Finished in 11.94 s (39 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 302,794 Reads with adapters: 6,233 (2.1%) Reads that were too short: 461 (0.2%) Reads written (passing filters): 5,772 (1.9%) Total basepairs processed: 75,758,094 bp Total written (filtered): 1,274,655 bp (1.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6233 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 2 0.0 3 0 0 2 16 22 0.0 3 0 21 1 17 3599 0.0 3 3517 79 3 18 12 0.0 3 2 10 19 3 0.0 3 0 0 2 1 20 4 0.0 3 1 0 0 3 22 1 0.0 3 1 24 1 0.0 3 1 28 1 0.0 3 1 29 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 38 2 0.0 3 0 0 0 2 41 1 0.0 3 1 46 3 0.0 3 0 0 0 3 47 2 0.0 3 0 0 0 2 48 2 0.0 3 0 0 1 1 51 8 0.0 3 0 0 0 8 52 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 104 0.0 3 0 0 0 104 74 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 80 4 0.0 3 0 0 0 4 85 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 0 3 92 2 0.0 3 0 0 0 2 93 7 0.0 3 0 0 4 3 97 41 0.0 3 0 0 0 41 98 5 0.0 3 0 0 0 5 99 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 10 0.0 3 0 0 0 10 103 2 0.0 3 0 0 0 2 107 1 0.0 3 1 116 2 0.0 3 0 0 0 2 117 11 0.0 3 0 0 0 11 118 8 0.0 3 0 0 0 8 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 8 0.0 3 0 0 0 8 122 557 0.0 3 0 0 4 553 123 161 0.0 3 0 0 1 160 124 32 0.0 3 0 0 0 32 125 82 0.0 3 0 0 0 82 126 41 0.0 3 0 0 0 41 127 17 0.0 3 0 0 1 16 128 11 0.0 3 0 0 0 11 129 4 0.0 3 0 0 0 4 130 5 0.0 3 0 0 0 5 131 4 0.0 3 0 0 0 4 132 1 0.0 3 0 0 0 1 133 7 0.0 3 0 0 0 7 134 9 0.0 3 0 0 0 9 135 3 0.0 3 0 0 0 3 136 4 0.0 3 0 0 0 4 137 10 0.0 3 0 0 0 10 138 18 0.0 3 0 0 0 18 139 8 0.0 3 0 0 0 8 140 2 0.0 3 0 0 0 2 141 3 0.0 3 0 0 0 3 142 442 0.0 3 0 0 0 442 143 3 0.0 3 0 0 0 3 144 4 0.0 3 0 0 0 4 145 48 0.0 3 0 0 2 46 146 21 0.0 3 0 0 0 21 147 4 0.0 3 0 0 0 4 148 10 0.0 3 0 0 0 10 149 10 0.0 3 0 0 0 10 150 3 0.0 3 0 0 0 3 151 97 0.0 3 0 0 1 96 152 18 0.0 3 0 0 0 18 153 6 0.0 3 0 0 0 6 154 16 0.0 3 0 0 0 16 155 3 0.0 3 0 0 0 3 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 165 4 0.0 3 0 0 0 4 167 1 0.0 3 0 0 0 1 168 7 0.0 3 0 0 0 7 169 2 0.0 3 0 0 0 2 170 11 0.0 3 0 0 0 11 171 101 0.0 3 0 0 0 101 172 121 0.0 3 0 0 1 120 173 3 0.0 3 0 0 0 3 174 10 0.0 3 0 0 2 8 175 9 0.0 3 0 0 0 9 177 3 0.0 3 0 0 0 3 178 1 0.0 3 0 0 0 1 180 3 0.0 3 0 1 0 2 183 1 0.0 3 0 0 0 1 186 1 0.0 3 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 190 3 0.0 3 0 0 0 3 192 1 0.0 3 0 0 0 1 193 6 0.0 3 0 0 0 6 197 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 202 15 0.0 3 0 0 0 15 203 3 0.0 3 0 0 0 3 205 3 0.0 3 0 0 0 3 206 2 0.0 3 0 0 0 2 207 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 215 4 0.0 3 0 0 0 4 216 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 222 3 0.0 3 0 0 0 3 224 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 231 2 0.0 3 0 0 0 2 232 1 0.0 3 0 0 0 1 233 2 0.0 3 0 0 0 2 234 66 0.0 3 0 0 0 66 235 18 0.0 3 0 0 0 18 236 19 0.0 3 0 0 0 19 237 22 0.0 3 0 0 1 21 238 26 0.0 3 0 0 1 25 239 10 0.0 3 0 0 0 10 240 2 0.0 3 0 0 0 2 241 2 0.0 3 0 0 0 2 242 7 0.0 3 0 0 0 7 243 11 0.0 3 1 0 0 10 244 41 0.0 3 0 0 4 37 245 1 0.0 3 0 0 0 1 247 2 0.0 3 0 0 0 2 248 2 0.0 3 0 0 0 2 250 28 0.0 3 0 0 0 28 251 3 0.0 3 0 1 0 2 252 26 0.0 3 0 0 0 26 261 2 0.0 3 0 0 0 2 262 5 0.0 3 0 0 0 5 263 9 0.0 3 0 0 0 9 264 4 0.0 3 0 0 0 4 265 2 0.0 3 0 0 0 2 266 4 0.0 3 0 0 0 4 267 4 0.0 3 0 0 1 3 272 2 0.0 3 0 0 0 2 273 9 0.0 3 0 0 1 8 274 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.19 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 5,772 Reads with adapters: 108 (1.9%) Reads written (passing filters): 5,772 (100.0%) Total basepairs processed: 1,274,655 bp Total written (filtered): 1,274,325 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 108 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 75.9% C: 14.8% G: 5.6% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 105 90.2 0 105 4 1 22.5 0 1 5 1 5.6 0 1 6 1 1.4 0 1