This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.60 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 192,694 (8.8%) Reads that were too short: 17,062 (0.8%) Reads written (passing filters): 175,632 (8.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 37,037,250 bp (5.6%) === Adapter 1 === Sequence: AAGGTC; Type: regular 5'; Length: 6; Trimmed: 192694 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1815 535.2 0 1815 7 3495 535.2 0 3495 8 156 535.2 0 156 9 2723 535.2 0 2723 10 51 535.2 0 51 11 70 535.2 0 70 12 130 535.2 0 130 13 66 535.2 0 66 14 66 535.2 0 66 15 95 535.2 0 95 16 99 535.2 0 99 17 65 535.2 0 65 18 73 535.2 0 73 19 68 535.2 0 68 20 80 535.2 0 80 21 81 535.2 0 81 22 62 535.2 0 62 23 63 535.2 0 63 24 96 535.2 0 96 25 86 535.2 0 86 26 55 535.2 0 55 27 87 535.2 0 87 28 87 535.2 0 87 29 58 535.2 0 58 30 48 535.2 0 48 31 64 535.2 0 64 32 74 535.2 0 74 33 84 535.2 0 84 34 85 535.2 0 85 35 94 535.2 0 94 36 94 535.2 0 94 37 71 535.2 0 71 38 79 535.2 0 79 39 79 535.2 0 79 40 99 535.2 0 99 41 83 535.2 0 83 42 97 535.2 0 97 43 96 535.2 0 96 44 81 535.2 0 81 45 105 535.2 0 105 46 91 535.2 0 91 47 89 535.2 0 89 48 96 535.2 0 96 49 85 535.2 0 85 50 58 535.2 0 58 51 74 535.2 0 74 52 61 535.2 0 61 53 92 535.2 0 92 54 132 535.2 0 132 55 120 535.2 0 120 56 91 535.2 0 91 57 118 535.2 0 118 58 73 535.2 0 73 59 82 535.2 0 82 60 113 535.2 0 113 61 83 535.2 0 83 62 181 535.2 0 181 63 114 535.2 0 114 64 118 535.2 0 118 65 91 535.2 0 91 66 136 535.2 0 136 67 93 535.2 0 93 68 128 535.2 0 128 69 172 535.2 0 172 70 183 535.2 0 183 71 244 535.2 0 244 72 623 535.2 0 623 73 1131 535.2 0 1131 74 386 535.2 0 386 75 392 535.2 0 392 76 710 535.2 0 710 77 358 535.2 0 358 78 321 535.2 0 321 79 703 535.2 0 703 80 737 535.2 0 737 81 830 535.2 0 830 82 832 535.2 0 832 83 1084 535.2 0 1084 84 1315 535.2 0 1315 85 2162 535.2 0 2162 86 30700 535.2 0 30700 87 53031 535.2 0 53031 88 42914 535.2 0 42914 89 201 535.2 0 201 90 72 535.2 0 72 91 44 535.2 0 44 92 70 535.2 0 70 93 58 535.2 0 58 94 66 535.2 0 66 95 67 535.2 0 67 96 74 535.2 0 74 97 65 535.2 0 65 98 58 535.2 0 58 99 73 535.2 0 73 100 77 535.2 0 77 101 72 535.2 0 72 102 81 535.2 0 81 103 215 535.2 0 215 104 232 535.2 0 232 105 175 535.2 0 175 106 47 535.2 0 47 107 45 535.2 0 45 108 72 535.2 0 72 109 44 535.2 0 44 110 74 535.2 0 74 111 73 535.2 0 73 112 62 535.2 0 62 113 68 535.2 0 68 114 64 535.2 0 64 115 89 535.2 0 89 116 71 535.2 0 71 117 62 535.2 0 62 118 78 535.2 0 78 119 83 535.2 0 83 120 75 535.2 0 75 121 78 535.2 0 78 122 99 535.2 0 99 123 249 535.2 0 249 124 455 535.2 0 455 125 928 535.2 0 928 126 572 535.2 0 572 127 282 535.2 0 282 128 132 535.2 0 132 129 108 535.2 0 108 130 141 535.2 0 141 131 160 535.2 0 160 132 110 535.2 0 110 133 100 535.2 0 100 134 135 535.2 0 135 135 108 535.2 0 108 136 159 535.2 0 159 137 106 535.2 0 106 138 112 535.2 0 112 139 122 535.2 0 122 140 104 535.2 0 104 141 219 535.2 0 219 142 202 535.2 0 202 143 211 535.2 0 211 144 1102 535.2 0 1102 145 1247 535.2 0 1247 146 3338 535.2 0 3338 147 280 535.2 0 280 148 81 535.2 0 81 149 124 535.2 0 124 150 145 535.2 0 145 151 135 535.2 0 135 152 74 535.2 0 74 153 71 535.2 0 71 154 80 535.2 0 80 155 112 535.2 0 112 156 122 535.2 0 122 157 80 535.2 0 80 158 90 535.2 0 90 159 61 535.2 0 61 160 67 535.2 0 67 161 70 535.2 0 70 162 56 535.2 0 56 163 71 535.2 0 71 164 72 535.2 0 72 165 95 535.2 0 95 166 381 535.2 0 381 167 207 535.2 0 207 168 211 535.2 0 211 169 65 535.2 0 65 170 93 535.2 0 93 171 91 535.2 0 91 172 119 535.2 0 119 173 83 535.2 0 83 174 83 535.2 0 83 175 125 535.2 0 125 176 98 535.2 0 98 177 73 535.2 0 73 178 73 535.2 0 73 179 64 535.2 0 64 180 105 535.2 0 105 181 105 535.2 0 105 182 137 535.2 0 137 183 132 535.2 0 132 184 153 535.2 0 153 185 908 535.2 0 908 186 1553 535.2 0 1553 187 1955 535.2 0 1955 188 157 535.2 0 157 189 57 535.2 0 57 190 69 535.2 0 69 191 71 535.2 0 71 192 103 535.2 0 103 193 153 535.2 0 153 194 146 535.2 0 146 195 159 535.2 0 159 196 110 535.2 0 110 197 75 535.2 0 75 198 64 535.2 0 64 199 74 535.2 0 74 200 112 535.2 0 112 201 89 535.2 0 89 202 88 535.2 0 88 203 82 535.2 0 82 204 92 535.2 0 92 205 123 535.2 0 123 206 823 535.2 0 823 207 925 535.2 0 925 208 1422 535.2 0 1422 209 104 535.2 0 104 210 193 535.2 0 193 211 458 535.2 0 458 212 649 535.2 0 649 213 369 535.2 0 369 214 84 535.2 0 84 215 56 535.2 0 56 216 70 535.2 0 70 217 77 535.2 0 77 218 69 535.2 0 69 219 75 535.2 0 75 220 100 535.2 0 100 221 93 535.2 0 93 222 107 535.2 0 107 223 52 535.2 0 52 224 85 535.2 0 85 225 72 535.2 0 72 226 129 535.2 0 129 227 148 535.2 0 148 228 201 535.2 0 201 229 99 535.2 0 99 230 65 535.2 0 65 231 100 535.2 0 100 232 74 535.2 0 74 233 68 535.2 0 68 234 84 535.2 0 84 235 61 535.2 0 61 236 53 535.2 0 53 237 68 535.2 0 68 238 70 535.2 0 70 239 70 535.2 0 70 240 78 535.2 0 78 241 106 535.2 0 106 242 131 535.2 0 131 243 131 535.2 0 131 244 79 535.2 0 79 245 97 535.2 0 97 246 165 535.2 0 165 247 91 535.2 0 91 248 88 535.2 0 88 249 66 535.2 0 66 250 86 535.2 0 86 251 72 535.2 0 72 252 78 535.2 0 78 253 61 535.2 0 61 254 70 535.2 0 70 255 175 535.2 0 175 256 186 535.2 0 186 257 207 535.2 0 207 258 119 535.2 0 119 259 667 535.2 0 667 260 1062 535.2 0 1062 261 1485 535.2 0 1485 262 246 535.2 0 246 263 77 535.2 0 77 264 77 535.2 0 77 265 101 535.2 0 101 266 102 535.2 0 102 267 212 535.2 0 212 268 230 535.2 0 230 269 145 535.2 0 145 270 80 535.2 0 80 271 56 535.2 0 56 272 77 535.2 0 77 273 66 535.2 0 66 274 65 535.2 0 65 275 124 535.2 0 124 276 142 535.2 0 142 277 103 535.2 0 103 278 85 535.2 0 85 279 97 535.2 0 97 280 130 535.2 0 130 281 160 535.2 0 160 282 135 535.2 0 135 283 123 535.2 0 123 284 51 535.2 0 51 285 78 535.2 0 78 286 46 535.2 0 46 287 73 535.2 0 73 288 70 535.2 0 70 289 95 535.2 0 95 290 66 535.2 0 66 291 70 535.2 0 70 292 67 535.2 0 67 293 69 535.2 0 69 294 138 535.2 0 138 295 110 535.2 0 110 296 117 535.2 0 117 297 73 535.2 0 73 298 66 535.2 0 66 299 61 535.2 0 61 300 93 535.2 0 93 301 228 535.2 0 228 Finished in 7.44 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 175,632 Reads with adapters: 3,849 (2.2%) Reads that were too short: 91 (0.1%) Reads written (passing filters): 3,758 (2.1%) Total basepairs processed: 37,037,250 bp Total written (filtered): 1,020,301 bp (2.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3849 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 18 2 0.0 3 0 0 2 19 73 0.0 3 0 69 4 20 2632 0.0 4 2438 150 30 7 7 21 30 0.0 4 0 21 5 3 1 22 943 0.0 4 845 69 19 7 3 23 8 0.0 4 1 1 0 2 4 25 9 0.0 4 0 0 0 5 4 29 1 0.0 4 0 0 0 0 1 48 9 0.0 4 0 0 0 0 9 50 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 62 1 0.0 4 1 73 7 0.0 4 0 0 0 0 7 74 35 0.0 4 0 0 0 0 35 86 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 93 5 0.0 4 0 0 0 0 5 94 3 0.0 4 0 0 0 0 3 95 12 0.0 4 0 0 0 0 12 128 7 0.0 4 0 0 0 0 7 129 4 0.0 4 0 0 0 0 4 131 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 142 2 0.0 4 0 0 0 0 2 145 1 0.0 4 0 0 0 0 1 146 3 0.0 4 0 0 0 0 3 148 1 0.0 4 0 0 0 0 1 149 5 0.0 4 0 0 0 0 5 150 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 154 5 0.0 4 0 0 0 0 5 155 4 0.0 4 0 0 0 0 4 156 2 0.0 4 0 0 0 0 2 158 2 0.0 4 0 0 0 0 2 161 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 175 2 0.0 4 0 0 0 0 2 177 1 0.0 4 0 0 0 0 1 178 4 0.0 4 0 0 0 0 4 179 2 0.0 4 0 0 0 0 2 180 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 204 4 0.0 4 0 0 0 0 4 206 1 0.0 4 0 0 0 0 1 209 2 0.0 4 0 0 0 0 2 210 1 0.0 4 0 0 0 0 1 211 2 0.0 4 0 0 0 0 2 212 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.13 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 3,758 Reads with adapters: 359 (9.6%) Reads written (passing filters): 3,758 (100.0%) Total basepairs processed: 1,020,301 bp Total written (filtered): 1,016,077 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 359 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.0% C: 12.5% G: 78.8% T: 3.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 14 58.7 0 14 4 2 14.7 0 2 9 40 0.0 0 4 36 10 158 0.0 1 13 145 11 88 0.0 1 6 82 12 47 0.0 1 0 47 13 5 0.0 1 0 5 15 1 0.0 1 0 1 150 2 0.0 1 2 151 1 0.0 1 1 171 1 0.0 1 1