This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 56.89 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 167,181 (7.6%) Reads that were too short: 13,150 (0.6%) Reads written (passing filters): 154,031 (7.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 32,400,189 bp (4.9%) === Adapter 1 === Sequence: AAGGTC; Type: regular 5'; Length: 6; Trimmed: 167181 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1431 535.2 0 1431 7 4127 535.2 0 4127 8 116 535.2 0 116 9 2757 535.2 0 2757 10 74 535.2 0 74 11 70 535.2 0 70 12 86 535.2 0 86 13 73 535.2 0 73 14 67 535.2 0 67 15 105 535.2 0 105 16 94 535.2 0 94 17 76 535.2 0 76 18 96 535.2 0 96 19 84 535.2 0 84 20 72 535.2 0 72 21 93 535.2 0 93 22 79 535.2 0 79 23 66 535.2 0 66 24 93 535.2 0 93 25 79 535.2 0 79 26 72 535.2 0 72 27 98 535.2 0 98 28 64 535.2 0 64 29 67 535.2 0 67 30 77 535.2 0 77 31 77 535.2 0 77 32 73 535.2 0 73 33 58 535.2 0 58 34 74 535.2 0 74 35 82 535.2 0 82 36 81 535.2 0 81 37 77 535.2 0 77 38 52 535.2 0 52 39 69 535.2 0 69 40 101 535.2 0 101 41 64 535.2 0 64 42 108 535.2 0 108 43 73 535.2 0 73 44 77 535.2 0 77 45 99 535.2 0 99 46 85 535.2 0 85 47 92 535.2 0 92 48 76 535.2 0 76 49 69 535.2 0 69 50 64 535.2 0 64 51 84 535.2 0 84 52 68 535.2 0 68 53 94 535.2 0 94 54 117 535.2 0 117 55 107 535.2 0 107 56 78 535.2 0 78 57 87 535.2 0 87 58 92 535.2 0 92 59 86 535.2 0 86 60 90 535.2 0 90 61 75 535.2 0 75 62 188 535.2 0 188 63 98 535.2 0 98 64 89 535.2 0 89 65 81 535.2 0 81 66 120 535.2 0 120 67 86 535.2 0 86 68 89 535.2 0 89 69 154 535.2 0 154 70 186 535.2 0 186 71 207 535.2 0 207 72 524 535.2 0 524 73 942 535.2 0 942 74 355 535.2 0 355 75 291 535.2 0 291 76 644 535.2 0 644 77 275 535.2 0 275 78 278 535.2 0 278 79 643 535.2 0 643 80 612 535.2 0 612 81 677 535.2 0 677 82 621 535.2 0 621 83 857 535.2 0 857 84 941 535.2 0 941 85 1520 535.2 0 1520 86 25403 535.2 0 25403 87 46471 535.2 0 46471 88 35859 535.2 0 35859 89 174 535.2 0 174 90 70 535.2 0 70 91 43 535.2 0 43 92 63 535.2 0 63 93 68 535.2 0 68 94 48 535.2 0 48 95 75 535.2 0 75 96 77 535.2 0 77 97 61 535.2 0 61 98 74 535.2 0 74 99 89 535.2 0 89 100 82 535.2 0 82 101 77 535.2 0 77 102 76 535.2 0 76 103 98 535.2 0 98 104 136 535.2 0 136 105 121 535.2 0 121 106 60 535.2 0 60 107 46 535.2 0 46 108 74 535.2 0 74 109 72 535.2 0 72 110 63 535.2 0 63 111 75 535.2 0 75 112 84 535.2 0 84 113 59 535.2 0 59 114 60 535.2 0 60 115 71 535.2 0 71 116 65 535.2 0 65 117 62 535.2 0 62 118 57 535.2 0 57 119 75 535.2 0 75 120 67 535.2 0 67 121 59 535.2 0 59 122 74 535.2 0 74 123 234 535.2 0 234 124 452 535.2 0 452 125 1007 535.2 0 1007 126 690 535.2 0 690 127 299 535.2 0 299 128 123 535.2 0 123 129 105 535.2 0 105 130 90 535.2 0 90 131 139 535.2 0 139 132 85 535.2 0 85 133 78 535.2 0 78 134 97 535.2 0 97 135 77 535.2 0 77 136 83 535.2 0 83 137 90 535.2 0 90 138 105 535.2 0 105 139 109 535.2 0 109 140 83 535.2 0 83 141 108 535.2 0 108 142 121 535.2 0 121 143 154 535.2 0 154 144 1172 535.2 0 1172 145 1287 535.2 0 1287 146 3411 535.2 0 3411 147 240 535.2 0 240 148 76 535.2 0 76 149 77 535.2 0 77 150 75 535.2 0 75 151 104 535.2 0 104 152 77 535.2 0 77 153 100 535.2 0 100 154 71 535.2 0 71 155 74 535.2 0 74 156 82 535.2 0 82 157 79 535.2 0 79 158 91 535.2 0 91 159 83 535.2 0 83 160 70 535.2 0 70 161 79 535.2 0 79 162 63 535.2 0 63 163 73 535.2 0 73 164 80 535.2 0 80 165 57 535.2 0 57 166 463 535.2 0 463 167 213 535.2 0 213 168 221 535.2 0 221 169 90 535.2 0 90 170 100 535.2 0 100 171 73 535.2 0 73 172 147 535.2 0 147 173 89 535.2 0 89 174 93 535.2 0 93 175 103 535.2 0 103 176 90 535.2 0 90 177 78 535.2 0 78 178 87 535.2 0 87 179 86 535.2 0 86 180 104 535.2 0 104 181 114 535.2 0 114 182 150 535.2 0 150 183 116 535.2 0 116 184 145 535.2 0 145 185 1039 535.2 0 1039 186 1756 535.2 0 1756 187 2098 535.2 0 2098 188 124 535.2 0 124 189 78 535.2 0 78 190 65 535.2 0 65 191 62 535.2 0 62 192 93 535.2 0 93 193 105 535.2 0 105 194 99 535.2 0 99 195 108 535.2 0 108 196 109 535.2 0 109 197 72 535.2 0 72 198 92 535.2 0 92 199 97 535.2 0 97 200 100 535.2 0 100 201 111 535.2 0 111 202 108 535.2 0 108 203 98 535.2 0 98 204 104 535.2 0 104 205 133 535.2 0 133 206 975 535.2 0 975 207 1206 535.2 0 1206 208 1849 535.2 0 1849 209 102 535.2 0 102 210 147 535.2 0 147 211 226 535.2 0 226 212 301 535.2 0 301 213 197 535.2 0 197 214 77 535.2 0 77 215 87 535.2 0 87 216 76 535.2 0 76 217 58 535.2 0 58 218 58 535.2 0 58 219 75 535.2 0 75 220 75 535.2 0 75 221 71 535.2 0 71 222 87 535.2 0 87 223 65 535.2 0 65 224 57 535.2 0 57 225 60 535.2 0 60 226 70 535.2 0 70 227 84 535.2 0 84 228 117 535.2 0 117 229 73 535.2 0 73 230 58 535.2 0 58 231 88 535.2 0 88 232 63 535.2 0 63 233 59 535.2 0 59 234 68 535.2 0 68 235 60 535.2 0 60 236 67 535.2 0 67 237 64 535.2 0 64 238 59 535.2 0 59 239 64 535.2 0 64 240 59 535.2 0 59 241 74 535.2 0 74 242 75 535.2 0 75 243 93 535.2 0 93 244 56 535.2 0 56 245 89 535.2 0 89 246 109 535.2 0 109 247 62 535.2 0 62 248 74 535.2 0 74 249 64 535.2 0 64 250 71 535.2 0 71 251 57 535.2 0 57 252 76 535.2 0 76 253 70 535.2 0 70 254 72 535.2 0 72 255 127 535.2 0 127 256 171 535.2 0 171 257 194 535.2 0 194 258 90 535.2 0 90 259 182 535.2 0 182 260 321 535.2 0 321 261 370 535.2 0 370 262 102 535.2 0 102 263 52 535.2 0 52 264 55 535.2 0 55 265 80 535.2 0 80 266 68 535.2 0 68 267 88 535.2 0 88 268 104 535.2 0 104 269 88 535.2 0 88 270 85 535.2 0 85 271 58 535.2 0 58 272 55 535.2 0 55 273 73 535.2 0 73 274 71 535.2 0 71 275 89 535.2 0 89 276 75 535.2 0 75 277 77 535.2 0 77 278 72 535.2 0 72 279 90 535.2 0 90 280 69 535.2 0 69 281 85 535.2 0 85 282 73 535.2 0 73 283 222 535.2 0 222 284 55 535.2 0 55 285 53 535.2 0 53 286 58 535.2 0 58 287 54 535.2 0 54 288 73 535.2 0 73 289 61 535.2 0 61 290 48 535.2 0 48 291 74 535.2 0 74 292 60 535.2 0 60 293 77 535.2 0 77 294 98 535.2 0 98 295 95 535.2 0 95 296 85 535.2 0 85 297 80 535.2 0 80 298 50 535.2 0 50 299 71 535.2 0 71 300 96 535.2 0 96 301 289 535.2 0 289 Finished in 5.71 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 154,031 Reads with adapters: 5,289 (3.4%) Reads that were too short: 81 (0.1%) Reads written (passing filters): 5,208 (3.4%) Total basepairs processed: 32,400,189 bp Total written (filtered): 1,222,841 bp (3.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5289 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 21 0.0 3 0 20 1 17 2711 0.0 3 2664 44 3 18 21 0.0 3 1 19 1 19 1172 0.0 3 1149 17 5 1 20 8 0.0 3 2 2 2 2 21 7 0.0 3 0 0 2 5 23 3 0.0 3 0 0 0 3 31 1 0.0 3 0 0 0 1 37 3 0.0 3 0 0 0 3 40 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 66 10 0.0 3 0 0 0 10 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 22 0.0 3 0 0 0 22 83 6 0.0 3 0 0 0 6 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 1 92 3 0.0 3 0 0 0 3 101 14 0.0 3 0 0 0 14 104 16 0.0 3 0 0 0 16 105 547 0.0 3 0 0 2 545 106 578 0.0 3 0 0 5 573 107 2 0.0 3 0 0 1 1 108 53 0.0 3 0 0 3 50 109 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 7 0.0 3 0 0 0 7 133 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 5 0.0 3 0 0 0 5 158 7 0.0 3 0 0 0 7 164 3 0.0 3 0 0 0 3 165 5 0.0 3 0 0 0 5 166 7 0.0 3 0 0 0 7 169 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 197 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 206 12 0.0 3 0 0 0 12 207 6 0.0 3 0 0 0 6 245 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 5,208 Reads with adapters: 24 (0.5%) Reads written (passing filters): 5,208 (100.0%) Total basepairs processed: 1,222,841 bp Total written (filtered): 1,222,765 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 24 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 45.8% C: 4.2% G: 45.8% T: 4.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 20 81.4 0 20 4 4 20.3 0 4