This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.21 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 110,103 (5.0%) Reads that were too short: 36,940 (1.7%) Reads written (passing filters): 73,163 (3.3%) Total basepairs processed: 659,880,795 bp Total written (filtered): 16,057,359 bp (2.4%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 110103 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3393 535.2 0 3393 7 2146 535.2 0 2146 8 7115 535.2 0 7115 9 6151 535.2 0 6151 10 110 535.2 0 110 11 116 535.2 0 116 12 103 535.2 0 103 13 112 535.2 0 112 14 116 535.2 0 116 15 163 535.2 0 163 16 146 535.2 0 146 17 131 535.2 0 131 18 117 535.2 0 117 19 139 535.2 0 139 20 133 535.2 0 133 21 121 535.2 0 121 22 105 535.2 0 105 23 106 535.2 0 106 24 125 535.2 0 125 25 104 535.2 0 104 26 138 535.2 0 138 27 157 535.2 0 157 28 135 535.2 0 135 29 124 535.2 0 124 30 135 535.2 0 135 31 141 535.2 0 141 32 164 535.2 0 164 33 194 535.2 0 194 34 142 535.2 0 142 35 194 535.2 0 194 36 171 535.2 0 171 37 1570 535.2 0 1570 38 2521 535.2 0 2521 39 3571 535.2 0 3571 40 160 535.2 0 160 41 180 535.2 0 180 42 150 535.2 0 150 43 152 535.2 0 152 44 146 535.2 0 146 45 126 535.2 0 126 46 103 535.2 0 103 47 117 535.2 0 117 48 128 535.2 0 128 49 163 535.2 0 163 50 116 535.2 0 116 51 131 535.2 0 131 52 119 535.2 0 119 53 120 535.2 0 120 54 144 535.2 0 144 55 116 535.2 0 116 56 123 535.2 0 123 57 129 535.2 0 129 58 119 535.2 0 119 59 124 535.2 0 124 60 131 535.2 0 131 61 112 535.2 0 112 62 128 535.2 0 128 63 150 535.2 0 150 64 113 535.2 0 113 65 108 535.2 0 108 66 135 535.2 0 135 67 93 535.2 0 93 68 112 535.2 0 112 69 135 535.2 0 135 70 158 535.2 0 158 71 153 535.2 0 153 72 187 535.2 0 187 73 128 535.2 0 128 74 141 535.2 0 141 75 116 535.2 0 116 76 184 535.2 0 184 77 154 535.2 0 154 78 152 535.2 0 152 79 181 535.2 0 181 80 160 535.2 0 160 81 105 535.2 0 105 82 153 535.2 0 153 83 108 535.2 0 108 84 116 535.2 0 116 85 103 535.2 0 103 86 101 535.2 0 101 87 103 535.2 0 103 88 136 535.2 0 136 89 168 535.2 0 168 90 170 535.2 0 170 91 392 535.2 0 392 92 687 535.2 0 687 93 1653 535.2 0 1653 94 894 535.2 0 894 95 190 535.2 0 190 96 146 535.2 0 146 97 161 535.2 0 161 98 113 535.2 0 113 99 110 535.2 0 110 100 120 535.2 0 120 101 131 535.2 0 131 102 100 535.2 0 100 103 132 535.2 0 132 104 196 535.2 0 196 105 202 535.2 0 202 106 150 535.2 0 150 107 122 535.2 0 122 108 133 535.2 0 133 109 160 535.2 0 160 110 147 535.2 0 147 111 137 535.2 0 137 112 217 535.2 0 217 113 227 535.2 0 227 114 333 535.2 0 333 115 289 535.2 0 289 116 172 535.2 0 172 117 164 535.2 0 164 118 183 535.2 0 183 119 115 535.2 0 115 120 130 535.2 0 130 121 140 535.2 0 140 122 179 535.2 0 179 123 153 535.2 0 153 124 148 535.2 0 148 125 145 535.2 0 145 126 135 535.2 0 135 127 226 535.2 0 226 128 272 535.2 0 272 129 604 535.2 0 604 130 770 535.2 0 770 131 840 535.2 0 840 132 238 535.2 0 238 133 192 535.2 0 192 134 221 535.2 0 221 135 203 535.2 0 203 136 311 535.2 0 311 137 275 535.2 0 275 138 276 535.2 0 276 139 274 535.2 0 274 140 291 535.2 0 291 141 315 535.2 0 315 142 3230 535.2 0 3230 143 3950 535.2 0 3950 144 7513 535.2 0 7513 145 187 535.2 0 187 146 118 535.2 0 118 147 136 535.2 0 136 148 130 535.2 0 130 149 182 535.2 0 182 150 216 535.2 0 216 151 165 535.2 0 165 152 112 535.2 0 112 153 148 535.2 0 148 154 137 535.2 0 137 155 124 535.2 0 124 156 230 535.2 0 230 157 115 535.2 0 115 158 124 535.2 0 124 159 122 535.2 0 122 160 119 535.2 0 119 161 122 535.2 0 122 162 127 535.2 0 127 163 127 535.2 0 127 164 137 535.2 0 137 165 101 535.2 0 101 166 108 535.2 0 108 167 96 535.2 0 96 168 150 535.2 0 150 169 165 535.2 0 165 170 191 535.2 0 191 171 93 535.2 0 93 172 90 535.2 0 90 173 119 535.2 0 119 174 129 535.2 0 129 175 93 535.2 0 93 176 124 535.2 0 124 177 171 535.2 0 171 178 162 535.2 0 162 179 173 535.2 0 173 180 108 535.2 0 108 181 80 535.2 0 80 182 144 535.2 0 144 183 97 535.2 0 97 184 91 535.2 0 91 185 134 535.2 0 134 186 132 535.2 0 132 187 116 535.2 0 116 188 102 535.2 0 102 189 117 535.2 0 117 190 131 535.2 0 131 191 148 535.2 0 148 192 138 535.2 0 138 193 108 535.2 0 108 194 109 535.2 0 109 195 102 535.2 0 102 196 86 535.2 0 86 197 110 535.2 0 110 198 108 535.2 0 108 199 114 535.2 0 114 200 103 535.2 0 103 201 166 535.2 0 166 202 152 535.2 0 152 203 160 535.2 0 160 204 175 535.2 0 175 205 156 535.2 0 156 206 139 535.2 0 139 207 118 535.2 0 118 208 153 535.2 0 153 209 317 535.2 0 317 210 350 535.2 0 350 211 456 535.2 0 456 212 377 535.2 0 377 213 335 535.2 0 335 214 2434 535.2 0 2434 215 3212 535.2 0 3212 216 3907 535.2 0 3907 217 310 535.2 0 310 218 214 535.2 0 214 219 113 535.2 0 113 220 133 535.2 0 133 221 160 535.2 0 160 222 135 535.2 0 135 223 143 535.2 0 143 224 160 535.2 0 160 225 155 535.2 0 155 226 162 535.2 0 162 227 180 535.2 0 180 228 294 535.2 0 294 229 546 535.2 0 546 230 522 535.2 0 522 231 623 535.2 0 623 232 222 535.2 0 222 233 292 535.2 0 292 234 1699 535.2 0 1699 235 3216 535.2 0 3216 236 3520 535.2 0 3520 237 963 535.2 0 963 238 111 535.2 0 111 239 97 535.2 0 97 240 82 535.2 0 82 241 87 535.2 0 87 242 76 535.2 0 76 243 119 535.2 0 119 244 121 535.2 0 121 245 103 535.2 0 103 246 88 535.2 0 88 247 109 535.2 0 109 248 90 535.2 0 90 249 107 535.2 0 107 250 102 535.2 0 102 251 86 535.2 0 86 252 84 535.2 0 84 253 115 535.2 0 115 254 136 535.2 0 136 255 137 535.2 0 137 256 143 535.2 0 143 257 82 535.2 0 82 258 90 535.2 0 90 259 98 535.2 0 98 260 121 535.2 0 121 261 118 535.2 0 118 262 134 535.2 0 134 263 171 535.2 0 171 264 134 535.2 0 134 265 121 535.2 0 121 266 118 535.2 0 118 267 100 535.2 0 100 268 83 535.2 0 83 269 80 535.2 0 80 270 124 535.2 0 124 271 176 535.2 0 176 272 179 535.2 0 179 273 82 535.2 0 82 274 96 535.2 0 96 275 129 535.2 0 129 276 101 535.2 0 101 277 79 535.2 0 79 278 78 535.2 0 78 279 91 535.2 0 91 280 84 535.2 0 84 281 114 535.2 0 114 282 123 535.2 0 123 283 160 535.2 0 160 284 208 535.2 0 208 285 127 535.2 0 127 286 94 535.2 0 94 287 84 535.2 0 84 288 78 535.2 0 78 289 103 535.2 0 103 290 90 535.2 0 90 291 213 535.2 0 213 292 248 535.2 0 248 293 322 535.2 0 322 294 100 535.2 0 100 295 186 535.2 0 186 296 442 535.2 0 442 297 719 535.2 0 719 298 997 535.2 0 997 299 658 535.2 0 658 300 179 535.2 0 179 301 830 535.2 0 830 Finished in 3.39 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 73,163 Reads with adapters: 8,461 (11.6%) Reads that were too short: 42 (0.1%) Reads written (passing filters): 8,419 (11.5%) Total basepairs processed: 16,057,359 bp Total written (filtered): 2,271,662 bp (14.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8461 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 2 2 18 6 0.0 3 0 0 2 1 3 19 74 0.0 3 0 51 10 10 3 20 6275 0.0 4 5698 434 91 40 12 21 1726 0.0 4 1548 134 31 11 2 22 11 0.0 4 1 5 3 0 2 23 114 0.0 4 65 8 0 35 6 24 11 0.0 4 1 0 0 6 4 29 1 0.0 4 0 0 0 0 1 38 1 0.0 4 1 42 40 0.0 4 1 0 0 0 39 45 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 1 61 1 0.0 4 0 0 0 0 1 62 2 0.0 4 0 0 0 0 2 63 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 97 74 0.0 4 0 0 0 0 74 99 3 0.0 4 0 0 0 1 2 100 2 0.0 4 0 0 0 0 2 103 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 53 0.0 4 0 0 0 0 53 128 14 0.0 4 0 0 0 0 14 143 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 150 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 162 2 0.0 4 0 0 0 0 2 166 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 198 4 0.0 4 0 0 0 0 4 199 4 0.0 4 0 0 0 0 4 205 2 0.0 4 0 0 0 0 2 206 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 1 0.0 4 0 0 0 0 1 230 3 0.0 4 0 0 0 0 3 249 1 0.0 4 0 0 0 0 1 252 5 0.0 4 0 0 0 0 5 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.30 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 8,419 Reads with adapters: 1,987 (23.6%) Reads written (passing filters): 8,419 (100.0%) Total basepairs processed: 2,271,662 bp Total written (filtered): 2,250,278 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1987 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.0% C: 9.1% G: 85.4% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 12 131.5 0 12 4 22 32.9 0 22 7 1 0.5 0 1 8 7 0.1 0 7 9 390 0.0 0 53 337 10 954 0.0 1 82 872 11 578 0.0 1 23 555 12 10 0.0 1 0 10 13 5 0.0 1 0 5 150 4 0.0 1 4 208 1 0.0 1 1 258 1 0.0 1 0 1 269 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.