This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 56.47 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 80,621 (3.7%) Reads that were too short: 16,956 (0.8%) Reads written (passing filters): 63,665 (2.9%) Total basepairs processed: 659,880,795 bp Total written (filtered): 13,945,053 bp (2.1%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 80621 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3067 535.2 0 3067 7 2563 535.2 0 2563 8 6953 535.2 0 6953 9 7130 535.2 0 7130 10 94 535.2 0 94 11 94 535.2 0 94 12 76 535.2 0 76 13 87 535.2 0 87 14 88 535.2 0 88 15 102 535.2 0 102 16 97 535.2 0 97 17 93 535.2 0 93 18 88 535.2 0 88 19 103 535.2 0 103 20 93 535.2 0 93 21 86 535.2 0 86 22 93 535.2 0 93 23 92 535.2 0 92 24 101 535.2 0 101 25 78 535.2 0 78 26 121 535.2 0 121 27 141 535.2 0 141 28 109 535.2 0 109 29 74 535.2 0 74 30 103 535.2 0 103 31 108 535.2 0 108 32 95 535.2 0 95 33 147 535.2 0 147 34 133 535.2 0 133 35 131 535.2 0 131 36 118 535.2 0 118 37 1091 535.2 0 1091 38 1002 535.2 0 1002 39 1722 535.2 0 1722 40 97 535.2 0 97 41 105 535.2 0 105 42 123 535.2 0 123 43 110 535.2 0 110 44 103 535.2 0 103 45 110 535.2 0 110 46 85 535.2 0 85 47 115 535.2 0 115 48 102 535.2 0 102 49 112 535.2 0 112 50 96 535.2 0 96 51 84 535.2 0 84 52 105 535.2 0 105 53 95 535.2 0 95 54 87 535.2 0 87 55 90 535.2 0 90 56 103 535.2 0 103 57 91 535.2 0 91 58 98 535.2 0 98 59 98 535.2 0 98 60 74 535.2 0 74 61 78 535.2 0 78 62 101 535.2 0 101 63 94 535.2 0 94 64 83 535.2 0 83 65 94 535.2 0 94 66 89 535.2 0 89 67 95 535.2 0 95 68 73 535.2 0 73 69 86 535.2 0 86 70 139 535.2 0 139 71 116 535.2 0 116 72 123 535.2 0 123 73 82 535.2 0 82 74 98 535.2 0 98 75 82 535.2 0 82 76 132 535.2 0 132 77 93 535.2 0 93 78 96 535.2 0 96 79 120 535.2 0 120 80 89 535.2 0 89 81 75 535.2 0 75 82 92 535.2 0 92 83 109 535.2 0 109 84 74 535.2 0 74 85 67 535.2 0 67 86 84 535.2 0 84 87 95 535.2 0 95 88 92 535.2 0 92 89 104 535.2 0 104 90 89 535.2 0 89 91 241 535.2 0 241 92 245 535.2 0 245 93 995 535.2 0 995 94 310 535.2 0 310 95 91 535.2 0 91 96 101 535.2 0 101 97 130 535.2 0 130 98 80 535.2 0 80 99 71 535.2 0 71 100 82 535.2 0 82 101 90 535.2 0 90 102 94 535.2 0 94 103 87 535.2 0 87 104 216 535.2 0 216 105 204 535.2 0 204 106 98 535.2 0 98 107 109 535.2 0 109 108 88 535.2 0 88 109 114 535.2 0 114 110 102 535.2 0 102 111 94 535.2 0 94 112 159 535.2 0 159 113 102 535.2 0 102 114 193 535.2 0 193 115 179 535.2 0 179 116 142 535.2 0 142 117 115 535.2 0 115 118 118 535.2 0 118 119 94 535.2 0 94 120 85 535.2 0 85 121 94 535.2 0 94 122 120 535.2 0 120 123 100 535.2 0 100 124 119 535.2 0 119 125 98 535.2 0 98 126 100 535.2 0 100 127 185 535.2 0 185 128 214 535.2 0 214 129 565 535.2 0 565 130 918 535.2 0 918 131 895 535.2 0 895 132 234 535.2 0 234 133 168 535.2 0 168 134 142 535.2 0 142 135 165 535.2 0 165 136 216 535.2 0 216 137 245 535.2 0 245 138 226 535.2 0 226 139 206 535.2 0 206 140 266 535.2 0 266 141 304 535.2 0 304 142 3633 535.2 0 3633 143 4653 535.2 0 4653 144 8623 535.2 0 8623 145 151 535.2 0 151 146 88 535.2 0 88 147 74 535.2 0 74 148 74 535.2 0 74 149 106 535.2 0 106 150 121 535.2 0 121 151 107 535.2 0 107 152 88 535.2 0 88 153 119 535.2 0 119 154 97 535.2 0 97 155 96 535.2 0 96 156 259 535.2 0 259 157 96 535.2 0 96 158 81 535.2 0 81 159 95 535.2 0 95 160 70 535.2 0 70 161 70 535.2 0 70 162 72 535.2 0 72 163 86 535.2 0 86 164 92 535.2 0 92 165 76 535.2 0 76 166 104 535.2 0 104 167 93 535.2 0 93 168 90 535.2 0 90 169 120 535.2 0 120 170 126 535.2 0 126 171 70 535.2 0 70 172 66 535.2 0 66 173 105 535.2 0 105 174 72 535.2 0 72 175 65 535.2 0 65 176 86 535.2 0 86 177 118 535.2 0 118 178 113 535.2 0 113 179 103 535.2 0 103 180 76 535.2 0 76 181 68 535.2 0 68 182 96 535.2 0 96 183 91 535.2 0 91 184 71 535.2 0 71 185 72 535.2 0 72 186 70 535.2 0 70 187 71 535.2 0 71 188 80 535.2 0 80 189 71 535.2 0 71 190 94 535.2 0 94 191 117 535.2 0 117 192 106 535.2 0 106 193 58 535.2 0 58 194 58 535.2 0 58 195 62 535.2 0 62 196 57 535.2 0 57 197 70 535.2 0 70 198 72 535.2 0 72 199 89 535.2 0 89 200 62 535.2 0 62 201 87 535.2 0 87 202 85 535.2 0 85 203 97 535.2 0 97 204 99 535.2 0 99 205 82 535.2 0 82 206 88 535.2 0 88 207 85 535.2 0 85 208 74 535.2 0 74 209 143 535.2 0 143 210 126 535.2 0 126 211 145 535.2 0 145 212 176 535.2 0 176 213 119 535.2 0 119 214 596 535.2 0 596 215 864 535.2 0 864 216 908 535.2 0 908 217 126 535.2 0 126 218 124 535.2 0 124 219 79 535.2 0 79 220 85 535.2 0 85 221 91 535.2 0 91 222 92 535.2 0 92 223 85 535.2 0 85 224 99 535.2 0 99 225 85 535.2 0 85 226 78 535.2 0 78 227 116 535.2 0 116 228 162 535.2 0 162 229 282 535.2 0 282 230 256 535.2 0 256 231 297 535.2 0 297 232 118 535.2 0 118 233 125 535.2 0 125 234 629 535.2 0 629 235 1231 535.2 0 1231 236 1396 535.2 0 1396 237 494 535.2 0 494 238 78 535.2 0 78 239 67 535.2 0 67 240 63 535.2 0 63 241 80 535.2 0 80 242 47 535.2 0 47 243 67 535.2 0 67 244 91 535.2 0 91 245 92 535.2 0 92 246 84 535.2 0 84 247 73 535.2 0 73 248 49 535.2 0 49 249 66 535.2 0 66 250 77 535.2 0 77 251 63 535.2 0 63 252 65 535.2 0 65 253 70 535.2 0 70 254 86 535.2 0 86 255 72 535.2 0 72 256 64 535.2 0 64 257 59 535.2 0 59 258 89 535.2 0 89 259 77 535.2 0 77 260 90 535.2 0 90 261 70 535.2 0 70 262 110 535.2 0 110 263 97 535.2 0 97 264 101 535.2 0 101 265 89 535.2 0 89 266 72 535.2 0 72 267 57 535.2 0 57 268 84 535.2 0 84 269 56 535.2 0 56 270 96 535.2 0 96 271 109 535.2 0 109 272 91 535.2 0 91 273 61 535.2 0 61 274 74 535.2 0 74 275 66 535.2 0 66 276 58 535.2 0 58 277 68 535.2 0 68 278 87 535.2 0 87 279 80 535.2 0 80 280 67 535.2 0 67 281 73 535.2 0 73 282 83 535.2 0 83 283 98 535.2 0 98 284 83 535.2 0 83 285 78 535.2 0 78 286 58 535.2 0 58 287 55 535.2 0 55 288 88 535.2 0 88 289 69 535.2 0 69 290 69 535.2 0 69 291 153 535.2 0 153 292 177 535.2 0 177 293 176 535.2 0 176 294 81 535.2 0 81 295 129 535.2 0 129 296 233 535.2 0 233 297 359 535.2 0 359 298 510 535.2 0 510 299 274 535.2 0 274 300 114 535.2 0 114 301 997 535.2 0 997 Finished in 2.61 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 63,665 Reads with adapters: 9,578 (15.0%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 9,562 (15.0%) Total basepairs processed: 13,945,053 bp Total written (filtered): 2,636,275 bp (18.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9578 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 43 0.0 3 0 42 1 17 6864 0.0 3 6690 172 2 18 2591 0.0 3 2527 61 3 19 16 0.0 3 1 8 4 3 20 5 0.0 3 1 0 0 4 21 3 0.0 3 0 0 0 3 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 1 39 1 0.0 3 0 0 0 1 42 4 0.0 3 0 0 0 4 48 2 0.0 3 0 0 0 2 50 9 0.0 3 0 0 0 9 56 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 3 0.0 3 0 0 0 3 79 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 143 1 0.0 3 1 148 1 0.0 3 0 0 0 1 154 4 0.0 3 0 0 0 4 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.34 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 9,562 Reads with adapters: 55 (0.6%) Reads written (passing filters): 9,562 (100.0%) Total basepairs processed: 2,636,275 bp Total written (filtered): 2,636,094 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 55 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 54.5% C: 9.1% G: 21.8% T: 14.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 46 149.4 0 46 4 8 37.4 0 8 11 1 0.0 1 0 1