This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.56 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 68,262 (3.1%) Reads that were too short: 12,168 (0.6%) Reads written (passing filters): 56,094 (2.6%) Total basepairs processed: 659,880,795 bp Total written (filtered): 13,009,922 bp (2.0%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 68262 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 346 535.2 0 346 7 17098 535.2 0 17098 8 13067 535.2 0 13067 9 59 535.2 0 59 10 35 535.2 0 35 11 31 535.2 0 31 12 58 535.2 0 58 13 34 535.2 0 34 14 39 535.2 0 39 15 35 535.2 0 35 16 62 535.2 0 62 17 41 535.2 0 41 18 49 535.2 0 49 19 52 535.2 0 52 20 61 535.2 0 61 21 51 535.2 0 51 22 84 535.2 0 84 23 45 535.2 0 45 24 35 535.2 0 35 25 37 535.2 0 37 26 47 535.2 0 47 27 77 535.2 0 77 28 66 535.2 0 66 29 68 535.2 0 68 30 214 535.2 0 214 31 71 535.2 0 71 32 68 535.2 0 68 33 32 535.2 0 32 34 41 535.2 0 41 35 35 535.2 0 35 36 41 535.2 0 41 37 51 535.2 0 51 38 41 535.2 0 41 39 40 535.2 0 40 40 60 535.2 0 60 41 43 535.2 0 43 42 36 535.2 0 36 43 34 535.2 0 34 44 58 535.2 0 58 45 39 535.2 0 39 46 56 535.2 0 56 47 38 535.2 0 38 48 35 535.2 0 35 49 42 535.2 0 42 50 37 535.2 0 37 51 34 535.2 0 34 52 66 535.2 0 66 53 48 535.2 0 48 54 93 535.2 0 93 55 144 535.2 0 144 56 179 535.2 0 179 57 34 535.2 0 34 58 37 535.2 0 37 59 47 535.2 0 47 60 66 535.2 0 66 61 379 535.2 0 379 62 384 535.2 0 384 63 653 535.2 0 653 64 49 535.2 0 49 65 40 535.2 0 40 66 31 535.2 0 31 67 96 535.2 0 96 68 109 535.2 0 109 69 146 535.2 0 146 70 44 535.2 0 44 71 60 535.2 0 60 72 110 535.2 0 110 73 117 535.2 0 117 74 54 535.2 0 54 75 41 535.2 0 41 76 52 535.2 0 52 77 38 535.2 0 38 78 83 535.2 0 83 79 87 535.2 0 87 80 142 535.2 0 142 81 52 535.2 0 52 82 42 535.2 0 42 83 64 535.2 0 64 84 63 535.2 0 63 85 55 535.2 0 55 86 75 535.2 0 75 87 81 535.2 0 81 88 70 535.2 0 70 89 40 535.2 0 40 90 43 535.2 0 43 91 40 535.2 0 40 92 35 535.2 0 35 93 34 535.2 0 34 94 42 535.2 0 42 95 37 535.2 0 37 96 53 535.2 0 53 97 47 535.2 0 47 98 66 535.2 0 66 99 33 535.2 0 33 100 44 535.2 0 44 101 42 535.2 0 42 102 31 535.2 0 31 103 34 535.2 0 34 104 22 535.2 0 22 105 35 535.2 0 35 106 53 535.2 0 53 107 41 535.2 0 41 108 39 535.2 0 39 109 38 535.2 0 38 110 36 535.2 0 36 111 55 535.2 0 55 112 63 535.2 0 63 113 61 535.2 0 61 114 74 535.2 0 74 115 71 535.2 0 71 116 53 535.2 0 53 117 44 535.2 0 44 118 31 535.2 0 31 119 55 535.2 0 55 120 37 535.2 0 37 121 54 535.2 0 54 122 61 535.2 0 61 123 113 535.2 0 113 124 117 535.2 0 117 125 169 535.2 0 169 126 162 535.2 0 162 127 185 535.2 0 185 128 301 535.2 0 301 129 502 535.2 0 502 130 453 535.2 0 453 131 256 535.2 0 256 132 74 535.2 0 74 133 60 535.2 0 60 134 160 535.2 0 160 135 101 535.2 0 101 136 107 535.2 0 107 137 56 535.2 0 56 138 63 535.2 0 63 139 78 535.2 0 78 140 74 535.2 0 74 141 58 535.2 0 58 142 51 535.2 0 51 143 60 535.2 0 60 144 191 535.2 0 191 145 410 535.2 0 410 146 307 535.2 0 307 147 198 535.2 0 198 148 62 535.2 0 62 149 161 535.2 0 161 150 50 535.2 0 50 151 54 535.2 0 54 152 63 535.2 0 63 153 35 535.2 0 35 154 39 535.2 0 39 155 54 535.2 0 54 156 153 535.2 0 153 157 175 535.2 0 175 158 60 535.2 0 60 159 60 535.2 0 60 160 48 535.2 0 48 161 35 535.2 0 35 162 46 535.2 0 46 163 34 535.2 0 34 164 41 535.2 0 41 165 69 535.2 0 69 166 249 535.2 0 249 167 164 535.2 0 164 168 195 535.2 0 195 169 39 535.2 0 39 170 37 535.2 0 37 171 53 535.2 0 53 172 51 535.2 0 51 173 35 535.2 0 35 174 66 535.2 0 66 175 70 535.2 0 70 176 71 535.2 0 71 177 48 535.2 0 48 178 60 535.2 0 60 179 45 535.2 0 45 180 55 535.2 0 55 181 42 535.2 0 42 182 81 535.2 0 81 183 70 535.2 0 70 184 92 535.2 0 92 185 122 535.2 0 122 186 76 535.2 0 76 187 80 535.2 0 80 188 67 535.2 0 67 189 77 535.2 0 77 190 77 535.2 0 77 191 82 535.2 0 82 192 132 535.2 0 132 193 151 535.2 0 151 194 263 535.2 0 263 195 2496 535.2 0 2496 196 4229 535.2 0 4229 197 2008 535.2 0 2008 198 253 535.2 0 253 199 608 535.2 0 608 200 54 535.2 0 54 201 53 535.2 0 53 202 65 535.2 0 65 203 50 535.2 0 50 204 31 535.2 0 31 205 60 535.2 0 60 206 53 535.2 0 53 207 82 535.2 0 82 208 101 535.2 0 101 209 72 535.2 0 72 210 89 535.2 0 89 211 225 535.2 0 225 212 191 535.2 0 191 213 154 535.2 0 154 214 60 535.2 0 60 215 76 535.2 0 76 216 81 535.2 0 81 217 122 535.2 0 122 218 150 535.2 0 150 219 313 535.2 0 313 220 343 535.2 0 343 221 234 535.2 0 234 222 194 535.2 0 194 223 67 535.2 0 67 224 80 535.2 0 80 225 67 535.2 0 67 226 112 535.2 0 112 227 97 535.2 0 97 228 70 535.2 0 70 229 52 535.2 0 52 230 46 535.2 0 46 231 62 535.2 0 62 232 72 535.2 0 72 233 94 535.2 0 94 234 53 535.2 0 53 235 66 535.2 0 66 236 61 535.2 0 61 237 48 535.2 0 48 238 49 535.2 0 49 239 76 535.2 0 76 240 149 535.2 0 149 241 155 535.2 0 155 242 173 535.2 0 173 243 155 535.2 0 155 244 145 535.2 0 145 245 168 535.2 0 168 246 266 535.2 0 266 247 170 535.2 0 170 248 101 535.2 0 101 249 129 535.2 0 129 250 192 535.2 0 192 251 145 535.2 0 145 252 55 535.2 0 55 253 50 535.2 0 50 254 89 535.2 0 89 255 107 535.2 0 107 256 117 535.2 0 117 257 82 535.2 0 82 258 58 535.2 0 58 259 70 535.2 0 70 260 80 535.2 0 80 261 89 535.2 0 89 262 180 535.2 0 180 263 191 535.2 0 191 264 305 535.2 0 305 265 96 535.2 0 96 266 84 535.2 0 84 267 360 535.2 0 360 268 669 535.2 0 669 269 675 535.2 0 675 270 174 535.2 0 174 271 51 535.2 0 51 272 81 535.2 0 81 273 219 535.2 0 219 274 243 535.2 0 243 275 234 535.2 0 234 276 153 535.2 0 153 277 87 535.2 0 87 278 63 535.2 0 63 279 61 535.2 0 61 280 61 535.2 0 61 281 103 535.2 0 103 282 117 535.2 0 117 283 99 535.2 0 99 284 47 535.2 0 47 285 39 535.2 0 39 286 45 535.2 0 45 287 75 535.2 0 75 288 109 535.2 0 109 289 96 535.2 0 96 290 89 535.2 0 89 291 53 535.2 0 53 292 44 535.2 0 44 293 70 535.2 0 70 294 69 535.2 0 69 295 78 535.2 0 78 296 74 535.2 0 74 297 62 535.2 0 62 298 61 535.2 0 61 299 52 535.2 0 52 300 58 535.2 0 58 301 78 535.2 0 78 Finished in 2.67 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 56,094 Reads with adapters: 14,008 (25.0%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 13,984 (24.9%) Total basepairs processed: 13,009,922 bp Total written (filtered): 3,811,137 bp (29.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14008 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 10 0.0 3 0 0 7 2 1 19 214 0.0 3 0 169 24 16 5 20 13566 0.0 4 12391 888 217 57 13 21 55 0.0 4 9 37 6 1 2 22 8 0.0 4 3 0 4 0 1 23 58 0.0 4 2 0 1 39 16 24 2 0.0 4 0 1 0 0 1 26 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 101 2 0.0 4 0 0 0 0 2 102 4 0.0 4 0 0 0 0 4 126 1 0.0 4 0 0 0 0 1 127 63 0.0 4 0 0 0 0 63 128 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.50 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 13,984 Reads with adapters: 2,553 (18.3%) Reads written (passing filters): 13,984 (100.0%) Total basepairs processed: 3,811,137 bp Total written (filtered): 3,782,500 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2553 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.2% C: 10.7% G: 83.6% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 31 218.5 0 31 4 26 54.6 0 26 5 2 13.7 0 2 7 1 0.9 0 1 9 62 0.1 0 3 59 10 420 0.0 1 33 387 11 1969 0.0 1 86 1883 12 24 0.0 1 0 24 13 1 0.0 1 0 1 14 7 0.0 1 0 7 15 1 0.0 1 0 1 122 1 0.0 1 0 1 151 4 0.0 1 4 209 2 0.0 1 1 1 224 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.