This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 56.20 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 64,240 (2.9%) Reads that were too short: 8,097 (0.4%) Reads written (passing filters): 56,143 (2.6%) Total basepairs processed: 659,880,795 bp Total written (filtered): 12,983,653 bp (2.0%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 64240 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 238 535.2 0 238 7 15245 535.2 0 15245 8 16590 535.2 0 16590 9 57 535.2 0 57 10 37 535.2 0 37 11 28 535.2 0 28 12 48 535.2 0 48 13 42 535.2 0 42 14 29 535.2 0 29 15 43 535.2 0 43 16 63 535.2 0 63 17 57 535.2 0 57 18 55 535.2 0 55 19 41 535.2 0 41 20 41 535.2 0 41 21 51 535.2 0 51 22 40 535.2 0 40 23 31 535.2 0 31 24 37 535.2 0 37 25 31 535.2 0 31 26 48 535.2 0 48 27 38 535.2 0 38 28 24 535.2 0 24 29 35 535.2 0 35 30 50 535.2 0 50 31 40 535.2 0 40 32 27 535.2 0 27 33 37 535.2 0 37 34 43 535.2 0 43 35 31 535.2 0 31 36 47 535.2 0 47 37 54 535.2 0 54 38 35 535.2 0 35 39 31 535.2 0 31 40 62 535.2 0 62 41 43 535.2 0 43 42 36 535.2 0 36 43 36 535.2 0 36 44 47 535.2 0 47 45 44 535.2 0 44 46 72 535.2 0 72 47 44 535.2 0 44 48 33 535.2 0 33 49 38 535.2 0 38 50 36 535.2 0 36 51 44 535.2 0 44 52 111 535.2 0 111 53 45 535.2 0 45 54 62 535.2 0 62 55 145 535.2 0 145 56 159 535.2 0 159 57 34 535.2 0 34 58 45 535.2 0 45 59 51 535.2 0 51 60 67 535.2 0 67 61 205 535.2 0 205 62 199 535.2 0 199 63 250 535.2 0 250 64 47 535.2 0 47 65 48 535.2 0 48 66 37 535.2 0 37 67 77 535.2 0 77 68 61 535.2 0 61 69 77 535.2 0 77 70 40 535.2 0 40 71 42 535.2 0 42 72 52 535.2 0 52 73 108 535.2 0 108 74 40 535.2 0 40 75 37 535.2 0 37 76 59 535.2 0 59 77 61 535.2 0 61 78 88 535.2 0 88 79 44 535.2 0 44 80 79 535.2 0 79 81 42 535.2 0 42 82 44 535.2 0 44 83 37 535.2 0 37 84 42 535.2 0 42 85 53 535.2 0 53 86 40 535.2 0 40 87 55 535.2 0 55 88 56 535.2 0 56 89 32 535.2 0 32 90 42 535.2 0 42 91 49 535.2 0 49 92 37 535.2 0 37 93 43 535.2 0 43 94 50 535.2 0 50 95 51 535.2 0 51 96 79 535.2 0 79 97 56 535.2 0 56 98 43 535.2 0 43 99 53 535.2 0 53 100 49 535.2 0 49 101 62 535.2 0 62 102 50 535.2 0 50 103 35 535.2 0 35 104 41 535.2 0 41 105 41 535.2 0 41 106 40 535.2 0 40 107 43 535.2 0 43 108 54 535.2 0 54 109 36 535.2 0 36 110 50 535.2 0 50 111 52 535.2 0 52 112 48 535.2 0 48 113 46 535.2 0 46 114 50 535.2 0 50 115 64 535.2 0 64 116 43 535.2 0 43 117 43 535.2 0 43 118 44 535.2 0 44 119 38 535.2 0 38 120 36 535.2 0 36 121 51 535.2 0 51 122 72 535.2 0 72 123 112 535.2 0 112 124 75 535.2 0 75 125 136 535.2 0 136 126 100 535.2 0 100 127 116 535.2 0 116 128 93 535.2 0 93 129 183 535.2 0 183 130 185 535.2 0 185 131 109 535.2 0 109 132 52 535.2 0 52 133 44 535.2 0 44 134 98 535.2 0 98 135 76 535.2 0 76 136 73 535.2 0 73 137 46 535.2 0 46 138 43 535.2 0 43 139 48 535.2 0 48 140 31 535.2 0 31 141 46 535.2 0 46 142 57 535.2 0 57 143 50 535.2 0 50 144 164 535.2 0 164 145 413 535.2 0 413 146 265 535.2 0 265 147 163 535.2 0 163 148 53 535.2 0 53 149 113 535.2 0 113 150 47 535.2 0 47 151 48 535.2 0 48 152 52 535.2 0 52 153 39 535.2 0 39 154 51 535.2 0 51 155 45 535.2 0 45 156 205 535.2 0 205 157 226 535.2 0 226 158 49 535.2 0 49 159 55 535.2 0 55 160 38 535.2 0 38 161 33 535.2 0 33 162 53 535.2 0 53 163 41 535.2 0 41 164 25 535.2 0 25 165 52 535.2 0 52 166 126 535.2 0 126 167 90 535.2 0 90 168 81 535.2 0 81 169 38 535.2 0 38 170 44 535.2 0 44 171 50 535.2 0 50 172 57 535.2 0 57 173 42 535.2 0 42 174 58 535.2 0 58 175 60 535.2 0 60 176 61 535.2 0 61 177 47 535.2 0 47 178 52 535.2 0 52 179 66 535.2 0 66 180 66 535.2 0 66 181 52 535.2 0 52 182 120 535.2 0 120 183 70 535.2 0 70 184 72 535.2 0 72 185 105 535.2 0 105 186 78 535.2 0 78 187 59 535.2 0 59 188 63 535.2 0 63 189 78 535.2 0 78 190 85 535.2 0 85 191 70 535.2 0 70 192 105 535.2 0 105 193 142 535.2 0 142 194 238 535.2 0 238 195 2828 535.2 0 2828 196 4971 535.2 0 4971 197 2491 535.2 0 2491 198 336 535.2 0 336 199 847 535.2 0 847 200 81 535.2 0 81 201 70 535.2 0 70 202 47 535.2 0 47 203 45 535.2 0 45 204 58 535.2 0 58 205 60 535.2 0 60 206 43 535.2 0 43 207 55 535.2 0 55 208 87 535.2 0 87 209 37 535.2 0 37 210 79 535.2 0 79 211 112 535.2 0 112 212 73 535.2 0 73 213 51 535.2 0 51 214 51 535.2 0 51 215 58 535.2 0 58 216 52 535.2 0 52 217 121 535.2 0 121 218 147 535.2 0 147 219 295 535.2 0 295 220 194 535.2 0 194 221 139 535.2 0 139 222 119 535.2 0 119 223 64 535.2 0 64 224 59 535.2 0 59 225 67 535.2 0 67 226 138 535.2 0 138 227 67 535.2 0 67 228 58 535.2 0 58 229 55 535.2 0 55 230 43 535.2 0 43 231 57 535.2 0 57 232 54 535.2 0 54 233 50 535.2 0 50 234 48 535.2 0 48 235 48 535.2 0 48 236 60 535.2 0 60 237 47 535.2 0 47 238 43 535.2 0 43 239 80 535.2 0 80 240 85 535.2 0 85 241 89 535.2 0 89 242 129 535.2 0 129 243 140 535.2 0 140 244 129 535.2 0 129 245 156 535.2 0 156 246 237 535.2 0 237 247 94 535.2 0 94 248 81 535.2 0 81 249 84 535.2 0 84 250 103 535.2 0 103 251 68 535.2 0 68 252 61 535.2 0 61 253 38 535.2 0 38 254 62 535.2 0 62 255 90 535.2 0 90 256 76 535.2 0 76 257 48 535.2 0 48 258 55 535.2 0 55 259 49 535.2 0 49 260 41 535.2 0 41 261 45 535.2 0 45 262 198 535.2 0 198 263 268 535.2 0 268 264 453 535.2 0 453 265 60 535.2 0 60 266 56 535.2 0 56 267 146 535.2 0 146 268 236 535.2 0 236 269 172 535.2 0 172 270 80 535.2 0 80 271 41 535.2 0 41 272 42 535.2 0 42 273 79 535.2 0 79 274 86 535.2 0 86 275 92 535.2 0 92 276 66 535.2 0 66 277 38 535.2 0 38 278 58 535.2 0 58 279 43 535.2 0 43 280 58 535.2 0 58 281 56 535.2 0 56 282 38 535.2 0 38 283 49 535.2 0 49 284 36 535.2 0 36 285 23 535.2 0 23 286 46 535.2 0 46 287 47 535.2 0 47 288 58 535.2 0 58 289 63 535.2 0 63 290 54 535.2 0 54 291 28 535.2 0 28 292 31 535.2 0 31 293 37 535.2 0 37 294 46 535.2 0 46 295 40 535.2 0 40 296 62 535.2 0 62 297 39 535.2 0 39 298 38 535.2 0 38 299 38 535.2 0 38 300 32 535.2 0 32 301 43 535.2 0 43 Finished in 2.43 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 56,143 Reads with adapters: 14,619 (26.0%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 14,601 (26.0%) Total basepairs processed: 12,983,653 bp Total written (filtered): 4,041,890 bp (31.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14619 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 5 0.0 3 0 0 5 16 105 0.0 3 0 103 2 17 14421 0.0 3 14170 244 6 1 18 26 0.0 3 2 23 1 19 7 0.0 3 0 0 7 20 19 0.0 3 0 0 0 19 34 1 0.0 3 1 37 1 0.0 3 0 0 0 1 42 7 0.0 3 0 0 0 7 44 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 75 8 0.0 3 0 0 0 8 76 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.51 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 14,601 Reads with adapters: 70 (0.5%) Reads written (passing filters): 14,601 (100.0%) Total basepairs processed: 4,041,890 bp Total written (filtered): 4,041,676 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 70 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 10.0% C: 2.9% G: 15.7% T: 71.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 67 228.1 0 67 4 2 57.0 0 2 5 1 14.3 0 1