This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 59.75 s (27 us/read; 2.20 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 127,225 (5.8%) Reads that were too short: 20,879 (1.0%) Reads written (passing filters): 106,346 (4.9%) Total basepairs processed: 659,880,795 bp Total written (filtered): 28,432,365 bp (4.3%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 127225 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1076 535.2 0 1076 7 39286 535.2 0 39286 8 936 535.2 0 936 9 42197 535.2 0 42197 10 73 535.2 0 73 11 17 535.2 0 17 12 15 535.2 0 15 13 16 535.2 0 16 14 12 535.2 0 12 15 15 535.2 0 15 16 20 535.2 0 20 17 9 535.2 0 9 18 12 535.2 0 12 19 12 535.2 0 12 20 10 535.2 0 10 21 13 535.2 0 13 22 26 535.2 0 26 23 49 535.2 0 49 24 13 535.2 0 13 25 9 535.2 0 9 26 12 535.2 0 12 27 9 535.2 0 9 28 14 535.2 0 14 29 20 535.2 0 20 30 14 535.2 0 14 31 9 535.2 0 9 32 10 535.2 0 10 33 16 535.2 0 16 34 14 535.2 0 14 35 19 535.2 0 19 36 34 535.2 0 34 37 14 535.2 0 14 38 22 535.2 0 22 39 22 535.2 0 22 40 35 535.2 0 35 41 117 535.2 0 117 42 101 535.2 0 101 43 86 535.2 0 86 44 17 535.2 0 17 45 8 535.2 0 8 46 14 535.2 0 14 47 30 535.2 0 30 48 14 535.2 0 14 49 23 535.2 0 23 50 28 535.2 0 28 51 25 535.2 0 25 52 34 535.2 0 34 53 36 535.2 0 36 54 13 535.2 0 13 55 29 535.2 0 29 56 20 535.2 0 20 57 29 535.2 0 29 58 26 535.2 0 26 59 14 535.2 0 14 60 22 535.2 0 22 61 16 535.2 0 16 62 16 535.2 0 16 63 29 535.2 0 29 64 17 535.2 0 17 65 33 535.2 0 33 66 24 535.2 0 24 67 27 535.2 0 27 68 30 535.2 0 30 69 157 535.2 0 157 70 102 535.2 0 102 71 123 535.2 0 123 72 27 535.2 0 27 73 29 535.2 0 29 74 38 535.2 0 38 75 23 535.2 0 23 76 31 535.2 0 31 77 27 535.2 0 27 78 21 535.2 0 21 79 24 535.2 0 24 80 44 535.2 0 44 81 40 535.2 0 40 82 72 535.2 0 72 83 41 535.2 0 41 84 51 535.2 0 51 85 46 535.2 0 46 86 68 535.2 0 68 87 203 535.2 0 203 88 72 535.2 0 72 89 129 535.2 0 129 90 151 535.2 0 151 91 93 535.2 0 93 92 72 535.2 0 72 93 67 535.2 0 67 94 85 535.2 0 85 95 70 535.2 0 70 96 71 535.2 0 71 97 109 535.2 0 109 98 100 535.2 0 100 99 91 535.2 0 91 100 1013 535.2 0 1013 101 1765 535.2 0 1765 102 3294 535.2 0 3294 103 423 535.2 0 423 104 60 535.2 0 60 105 33 535.2 0 33 106 41 535.2 0 41 107 47 535.2 0 47 108 37 535.2 0 37 109 29 535.2 0 29 110 55 535.2 0 55 111 37 535.2 0 37 112 46 535.2 0 46 113 49 535.2 0 49 114 25 535.2 0 25 115 41 535.2 0 41 116 36 535.2 0 36 117 50 535.2 0 50 118 88 535.2 0 88 119 44 535.2 0 44 120 51 535.2 0 51 121 42 535.2 0 42 122 82 535.2 0 82 123 121 535.2 0 121 124 94 535.2 0 94 125 78 535.2 0 78 126 57 535.2 0 57 127 33 535.2 0 33 128 55 535.2 0 55 129 53 535.2 0 53 130 80 535.2 0 80 131 219 535.2 0 219 132 353 535.2 0 353 133 595 535.2 0 595 134 236 535.2 0 236 135 74 535.2 0 74 136 75 535.2 0 75 137 130 535.2 0 130 138 136 535.2 0 136 139 219 535.2 0 219 140 135 535.2 0 135 141 61 535.2 0 61 142 105 535.2 0 105 143 223 535.2 0 223 144 256 535.2 0 256 145 211 535.2 0 211 146 63 535.2 0 63 147 70 535.2 0 70 148 76 535.2 0 76 149 77 535.2 0 77 150 73 535.2 0 73 151 76 535.2 0 76 152 86 535.2 0 86 153 93 535.2 0 93 154 59 535.2 0 59 155 59 535.2 0 59 156 80 535.2 0 80 157 253 535.2 0 253 158 502 535.2 0 502 159 429 535.2 0 429 160 281 535.2 0 281 161 149 535.2 0 149 162 232 535.2 0 232 163 66 535.2 0 66 164 77 535.2 0 77 165 58 535.2 0 58 166 40 535.2 0 40 167 77 535.2 0 77 168 114 535.2 0 114 169 133 535.2 0 133 170 100 535.2 0 100 171 65 535.2 0 65 172 60 535.2 0 60 173 83 535.2 0 83 174 64 535.2 0 64 175 61 535.2 0 61 176 577 535.2 0 577 177 542 535.2 0 542 178 838 535.2 0 838 179 190 535.2 0 190 180 77 535.2 0 77 181 89 535.2 0 89 182 111 535.2 0 111 183 156 535.2 0 156 184 179 535.2 0 179 185 90 535.2 0 90 186 64 535.2 0 64 187 103 535.2 0 103 188 140 535.2 0 140 189 164 535.2 0 164 190 121 535.2 0 121 191 117 535.2 0 117 192 44 535.2 0 44 193 41 535.2 0 41 194 56 535.2 0 56 195 75 535.2 0 75 196 115 535.2 0 115 197 150 535.2 0 150 198 158 535.2 0 158 199 123 535.2 0 123 200 75 535.2 0 75 201 73 535.2 0 73 202 125 535.2 0 125 203 232 535.2 0 232 204 231 535.2 0 231 205 125 535.2 0 125 206 89 535.2 0 89 207 74 535.2 0 74 208 74 535.2 0 74 209 88 535.2 0 88 210 152 535.2 0 152 211 211 535.2 0 211 212 190 535.2 0 190 213 153 535.2 0 153 214 121 535.2 0 121 215 122 535.2 0 122 216 119 535.2 0 119 217 110 535.2 0 110 218 143 535.2 0 143 219 142 535.2 0 142 220 108 535.2 0 108 221 87 535.2 0 87 222 206 535.2 0 206 223 478 535.2 0 478 224 560 535.2 0 560 225 283 535.2 0 283 226 192 535.2 0 192 227 119 535.2 0 119 228 123 535.2 0 123 229 142 535.2 0 142 230 109 535.2 0 109 231 90 535.2 0 90 232 86 535.2 0 86 233 105 535.2 0 105 234 140 535.2 0 140 235 138 535.2 0 138 236 130 535.2 0 130 237 130 535.2 0 130 238 209 535.2 0 209 239 249 535.2 0 249 240 204 535.2 0 204 241 114 535.2 0 114 242 230 535.2 0 230 243 331 535.2 0 331 244 385 535.2 0 385 245 233 535.2 0 233 246 229 535.2 0 229 247 191 535.2 0 191 248 214 535.2 0 214 249 123 535.2 0 123 250 99 535.2 0 99 251 137 535.2 0 137 252 108 535.2 0 108 253 118 535.2 0 118 254 136 535.2 0 136 255 237 535.2 0 237 256 258 535.2 0 258 257 243 535.2 0 243 258 219 535.2 0 219 259 160 535.2 0 160 260 143 535.2 0 143 261 150 535.2 0 150 262 253 535.2 0 253 263 439 535.2 0 439 264 655 535.2 0 655 265 522 535.2 0 522 266 468 535.2 0 468 267 227 535.2 0 227 268 115 535.2 0 115 269 145 535.2 0 145 270 231 535.2 0 231 271 331 535.2 0 331 272 357 535.2 0 357 273 296 535.2 0 296 274 346 535.2 0 346 275 160 535.2 0 160 276 240 535.2 0 240 277 233 535.2 0 233 278 265 535.2 0 265 279 220 535.2 0 220 280 138 535.2 0 138 281 149 535.2 0 149 282 174 535.2 0 174 283 267 535.2 0 267 284 230 535.2 0 230 285 251 535.2 0 251 286 237 535.2 0 237 287 260 535.2 0 260 288 209 535.2 0 209 289 160 535.2 0 160 290 143 535.2 0 143 291 290 535.2 0 290 292 413 535.2 0 413 293 320 535.2 0 320 294 197 535.2 0 197 295 143 535.2 0 143 296 87 535.2 0 87 297 144 535.2 0 144 298 186 535.2 0 186 299 220 535.2 0 220 300 297 535.2 0 297 301 414 535.2 0 414 Finished in 5.44 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 106,346 Reads with adapters: 39,881 (37.5%) Reads that were too short: 92 (0.1%) Reads written (passing filters): 39,789 (37.4%) Total basepairs processed: 28,432,365 bp Total written (filtered): 10,893,405 bp (38.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 39881 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 6 0.0 3 0 0 0 4 2 18 19 0.0 3 0 0 7 8 4 19 490 0.0 3 0 386 53 39 12 20 38834 0.0 4 35648 2420 555 161 50 21 98 0.0 4 19 73 1 2 3 22 9 0.0 4 0 2 5 1 1 23 249 0.0 4 108 13 2 87 39 28 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 34 2 0.0 4 0 0 0 0 2 42 1 0.0 4 1 52 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 69 1 0.0 4 1 70 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 84 2 0.0 4 0 0 0 0 2 92 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 94 2 0.0 4 0 0 0 0 2 95 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 113 2 0.0 4 1 0 0 0 1 115 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 119 3 0.0 4 0 0 0 0 3 120 2 0.0 4 0 0 0 0 2 121 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 61 0.0 4 0 0 0 0 61 128 1 0.0 4 0 0 0 0 1 134 4 0.0 4 0 0 0 0 4 135 3 0.0 4 0 0 0 1 2 136 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 153 3 0.0 4 0 0 0 0 3 154 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 181 3 0.0 4 0 0 0 0 3 188 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 213 2 0.0 4 0 0 0 0 2 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 218 2 0.0 4 0 0 0 0 2 228 2 0.0 4 0 0 0 0 2 229 10 0.0 4 0 0 0 0 10 230 6 0.0 4 0 0 0 0 6 231 3 0.0 4 0 0 0 0 3 232 9 0.0 4 0 0 0 0 9 233 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 278 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.39 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 39,789 Reads with adapters: 3,293 (8.3%) Reads written (passing filters): 39,789 (100.0%) Total basepairs processed: 10,893,405 bp Total written (filtered): 10,852,287 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3293 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.8% C: 13.5% G: 77.2% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 156 621.7 0 156 4 45 155.4 0 45 5 1 38.9 0 1 6 1 9.7 0 1 9 20 0.2 0 3 17 10 131 0.0 1 3 128 11 2576 0.0 1 144 2432 12 285 0.0 1 11 274 13 36 0.0 1 0 36 14 3 0.0 1 0 3 15 3 0.0 1 0 3 16 1 0.0 1 0 1 145 7 0.0 1 5 2 146 1 0.0 1 1 152 4 0.0 1 2 2 191 2 0.0 1 0 2 210 17 0.0 1 13 4 211 1 0.0 1 0 1 222 1 0.0 1 0 1 225 1 0.0 1 1 263 1 0.0 1 1