This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 59.10 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 105,477 (4.8%) Reads that were too short: 7,993 (0.4%) Reads written (passing filters): 97,484 (4.4%) Total basepairs processed: 659,880,795 bp Total written (filtered): 27,450,804 bp (4.2%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 105477 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 881 535.2 0 881 7 47311 535.2 0 47311 8 685 535.2 0 685 9 39259 535.2 0 39259 10 46 535.2 0 46 11 23 535.2 0 23 12 16 535.2 0 16 13 12 535.2 0 12 14 12 535.2 0 12 15 61 535.2 0 61 16 103 535.2 0 103 17 10 535.2 0 10 18 10 535.2 0 10 19 11 535.2 0 11 20 22 535.2 0 22 21 14 535.2 0 14 22 19 535.2 0 19 23 21 535.2 0 21 24 17 535.2 0 17 25 19 535.2 0 19 26 10 535.2 0 10 27 13 535.2 0 13 28 16 535.2 0 16 29 21 535.2 0 21 30 16 535.2 0 16 31 10 535.2 0 10 32 26 535.2 0 26 33 21 535.2 0 21 34 16 535.2 0 16 35 26 535.2 0 26 36 18 535.2 0 18 37 14 535.2 0 14 38 21 535.2 0 21 39 21 535.2 0 21 40 18 535.2 0 18 41 34 535.2 0 34 42 24 535.2 0 24 43 12 535.2 0 12 44 16 535.2 0 16 45 19 535.2 0 19 46 12 535.2 0 12 47 29 535.2 0 29 48 12 535.2 0 12 49 17 535.2 0 17 50 19 535.2 0 19 51 30 535.2 0 30 52 18 535.2 0 18 53 21 535.2 0 21 54 17 535.2 0 17 55 25 535.2 0 25 56 12 535.2 0 12 57 15 535.2 0 15 58 26 535.2 0 26 59 18 535.2 0 18 60 15 535.2 0 15 61 18 535.2 0 18 62 17 535.2 0 17 63 29 535.2 0 29 64 22 535.2 0 22 65 19 535.2 0 19 66 16 535.2 0 16 67 16 535.2 0 16 68 24 535.2 0 24 69 61 535.2 0 61 70 22 535.2 0 22 71 52 535.2 0 52 72 16 535.2 0 16 73 18 535.2 0 18 74 16 535.2 0 16 75 11 535.2 0 11 76 19 535.2 0 19 77 28 535.2 0 28 78 22 535.2 0 22 79 25 535.2 0 25 80 28 535.2 0 28 81 23 535.2 0 23 82 36 535.2 0 36 83 25 535.2 0 25 84 33 535.2 0 33 85 38 535.2 0 38 86 32 535.2 0 32 87 78 535.2 0 78 88 52 535.2 0 52 89 39 535.2 0 39 90 44 535.2 0 44 91 43 535.2 0 43 92 42 535.2 0 42 93 31 535.2 0 31 94 32 535.2 0 32 95 21 535.2 0 21 96 24 535.2 0 24 97 29 535.2 0 29 98 25 535.2 0 25 99 30 535.2 0 30 100 151 535.2 0 151 101 277 535.2 0 277 102 589 535.2 0 589 103 117 535.2 0 117 104 28 535.2 0 28 105 21 535.2 0 21 106 21 535.2 0 21 107 36 535.2 0 36 108 27 535.2 0 27 109 26 535.2 0 26 110 38 535.2 0 38 111 18 535.2 0 18 112 34 535.2 0 34 113 26 535.2 0 26 114 27 535.2 0 27 115 23 535.2 0 23 116 32 535.2 0 32 117 29 535.2 0 29 118 39 535.2 0 39 119 25 535.2 0 25 120 30 535.2 0 30 121 21 535.2 0 21 122 50 535.2 0 50 123 46 535.2 0 46 124 21 535.2 0 21 125 42 535.2 0 42 126 35 535.2 0 35 127 22 535.2 0 22 128 19 535.2 0 19 129 33 535.2 0 33 130 37 535.2 0 37 131 74 535.2 0 74 132 128 535.2 0 128 133 235 535.2 0 235 134 97 535.2 0 97 135 34 535.2 0 34 136 34 535.2 0 34 137 44 535.2 0 44 138 44 535.2 0 44 139 88 535.2 0 88 140 76 535.2 0 76 141 19 535.2 0 19 142 40 535.2 0 40 143 58 535.2 0 58 144 78 535.2 0 78 145 71 535.2 0 71 146 27 535.2 0 27 147 39 535.2 0 39 148 24 535.2 0 24 149 27 535.2 0 27 150 40 535.2 0 40 151 29 535.2 0 29 152 48 535.2 0 48 153 48 535.2 0 48 154 38 535.2 0 38 155 32 535.2 0 32 156 36 535.2 0 36 157 87 535.2 0 87 158 187 535.2 0 187 159 200 535.2 0 200 160 145 535.2 0 145 161 70 535.2 0 70 162 89 535.2 0 89 163 42 535.2 0 42 164 34 535.2 0 34 165 19 535.2 0 19 166 18 535.2 0 18 167 33 535.2 0 33 168 49 535.2 0 49 169 57 535.2 0 57 170 42 535.2 0 42 171 30 535.2 0 30 172 44 535.2 0 44 173 28 535.2 0 28 174 51 535.2 0 51 175 34 535.2 0 34 176 437 535.2 0 437 177 364 535.2 0 364 178 583 535.2 0 583 179 52 535.2 0 52 180 37 535.2 0 37 181 34 535.2 0 34 182 44 535.2 0 44 183 42 535.2 0 42 184 46 535.2 0 46 185 51 535.2 0 51 186 33 535.2 0 33 187 48 535.2 0 48 188 80 535.2 0 80 189 96 535.2 0 96 190 62 535.2 0 62 191 58 535.2 0 58 192 35 535.2 0 35 193 30 535.2 0 30 194 28 535.2 0 28 195 38 535.2 0 38 196 28 535.2 0 28 197 53 535.2 0 53 198 48 535.2 0 48 199 42 535.2 0 42 200 21 535.2 0 21 201 21 535.2 0 21 202 31 535.2 0 31 203 46 535.2 0 46 204 69 535.2 0 69 205 34 535.2 0 34 206 38 535.2 0 38 207 53 535.2 0 53 208 37 535.2 0 37 209 34 535.2 0 34 210 64 535.2 0 64 211 99 535.2 0 99 212 80 535.2 0 80 213 103 535.2 0 103 214 37 535.2 0 37 215 48 535.2 0 48 216 42 535.2 0 42 217 52 535.2 0 52 218 33 535.2 0 33 219 47 535.2 0 47 220 40 535.2 0 40 221 31 535.2 0 31 222 79 535.2 0 79 223 96 535.2 0 96 224 120 535.2 0 120 225 84 535.2 0 84 226 134 535.2 0 134 227 68 535.2 0 68 228 50 535.2 0 50 229 68 535.2 0 68 230 46 535.2 0 46 231 50 535.2 0 50 232 49 535.2 0 49 233 54 535.2 0 54 234 51 535.2 0 51 235 58 535.2 0 58 236 59 535.2 0 59 237 54 535.2 0 54 238 128 535.2 0 128 239 156 535.2 0 156 240 103 535.2 0 103 241 62 535.2 0 62 242 75 535.2 0 75 243 81 535.2 0 81 244 88 535.2 0 88 245 86 535.2 0 86 246 70 535.2 0 70 247 76 535.2 0 76 248 83 535.2 0 83 249 75 535.2 0 75 250 51 535.2 0 51 251 68 535.2 0 68 252 55 535.2 0 55 253 75 535.2 0 75 254 55 535.2 0 55 255 55 535.2 0 55 256 94 535.2 0 94 257 80 535.2 0 80 258 71 535.2 0 71 259 50 535.2 0 50 260 55 535.2 0 55 261 45 535.2 0 45 262 104 535.2 0 104 263 162 535.2 0 162 264 252 535.2 0 252 265 141 535.2 0 141 266 146 535.2 0 146 267 60 535.2 0 60 268 48 535.2 0 48 269 63 535.2 0 63 270 68 535.2 0 68 271 90 535.2 0 90 272 106 535.2 0 106 273 81 535.2 0 81 274 274 535.2 0 274 275 90 535.2 0 90 276 123 535.2 0 123 277 84 535.2 0 84 278 77 535.2 0 77 279 61 535.2 0 61 280 66 535.2 0 66 281 58 535.2 0 58 282 80 535.2 0 80 283 83 535.2 0 83 284 89 535.2 0 89 285 91 535.2 0 91 286 90 535.2 0 90 287 99 535.2 0 99 288 90 535.2 0 90 289 73 535.2 0 73 290 77 535.2 0 77 291 84 535.2 0 84 292 114 535.2 0 114 293 93 535.2 0 93 294 82 535.2 0 82 295 57 535.2 0 57 296 38 535.2 0 38 297 76 535.2 0 76 298 92 535.2 0 92 299 109 535.2 0 109 300 152 535.2 0 152 301 195 535.2 0 195 Finished in 4.57 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 97,484 Reads with adapters: 40,180 (41.2%) Reads that were too short: 21 (0.0%) Reads written (passing filters): 40,159 (41.2%) Total basepairs processed: 27,450,804 bp Total written (filtered): 11,038,523 bp (40.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 40180 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 9 0.0 3 0 0 9 16 319 0.0 3 0 315 4 17 39649 0.0 3 38854 762 32 1 18 78 0.0 3 4 71 3 19 18 0.0 3 4 1 12 1 20 37 0.0 3 21 0 0 16 22 1 0.0 3 0 0 0 1 25 1 0.0 3 1 28 1 0.0 3 1 30 1 0.0 3 1 35 1 0.0 3 1 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 1 41 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 59 1 0.0 3 1 69 2 0.0 3 0 0 0 2 71 11 0.0 3 0 0 0 11 72 2 0.0 3 0 0 0 2 74 6 0.0 3 0 0 0 6 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 178 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 185 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 3 0.0 3 0 0 0 3 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.46 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 40,159 Reads with adapters: 3,781 (9.4%) Reads written (passing filters): 40,159 (100.0%) Total basepairs processed: 11,038,523 bp Total written (filtered): 11,027,112 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3781 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 81.6% C: 7.5% G: 10.2% T: 0.7% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3718 627.5 0 3718 4 62 156.9 0 62 9 1 0.2 0 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.