This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.11 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 62,730 (2.9%) Reads that were too short: 33,273 (1.5%) Reads written (passing filters): 29,457 (1.3%) Total basepairs processed: 659,880,795 bp Total written (filtered): 6,846,242 bp (1.0%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 62730 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2096 535.2 0 2096 7 251 535.2 0 251 8 5395 535.2 0 5395 9 4635 535.2 0 4635 10 57 535.2 0 57 11 80 535.2 0 80 12 106 535.2 0 106 13 78 535.2 0 78 14 52 535.2 0 52 15 49 535.2 0 49 16 59 535.2 0 59 17 62 535.2 0 62 18 65 535.2 0 65 19 75 535.2 0 75 20 56 535.2 0 56 21 51 535.2 0 51 22 78 535.2 0 78 23 75 535.2 0 75 24 91 535.2 0 91 25 60 535.2 0 60 26 53 535.2 0 53 27 47 535.2 0 47 28 46 535.2 0 46 29 66 535.2 0 66 30 51 535.2 0 51 31 61 535.2 0 61 32 66 535.2 0 66 33 61 535.2 0 61 34 59 535.2 0 59 35 64 535.2 0 64 36 65 535.2 0 65 37 50 535.2 0 50 38 74 535.2 0 74 39 85 535.2 0 85 40 100 535.2 0 100 41 103 535.2 0 103 42 88 535.2 0 88 43 56 535.2 0 56 44 48 535.2 0 48 45 69 535.2 0 69 46 66 535.2 0 66 47 53 535.2 0 53 48 60 535.2 0 60 49 55 535.2 0 55 50 54 535.2 0 54 51 109 535.2 0 109 52 99 535.2 0 99 53 234 535.2 0 234 54 76 535.2 0 76 55 57 535.2 0 57 56 52 535.2 0 52 57 54 535.2 0 54 58 71 535.2 0 71 59 72 535.2 0 72 60 48 535.2 0 48 61 78 535.2 0 78 62 78 535.2 0 78 63 63 535.2 0 63 64 50 535.2 0 50 65 53 535.2 0 53 66 68 535.2 0 68 67 64 535.2 0 64 68 58 535.2 0 58 69 66 535.2 0 66 70 46 535.2 0 46 71 55 535.2 0 55 72 64 535.2 0 64 73 66 535.2 0 66 74 75 535.2 0 75 75 96 535.2 0 96 76 64 535.2 0 64 77 83 535.2 0 83 78 61 535.2 0 61 79 70 535.2 0 70 80 63 535.2 0 63 81 74 535.2 0 74 82 81 535.2 0 81 83 76 535.2 0 76 84 64 535.2 0 64 85 57 535.2 0 57 86 44 535.2 0 44 87 75 535.2 0 75 88 50 535.2 0 50 89 68 535.2 0 68 90 81 535.2 0 81 91 97 535.2 0 97 92 80 535.2 0 80 93 91 535.2 0 91 94 95 535.2 0 95 95 66 535.2 0 66 96 73 535.2 0 73 97 86 535.2 0 86 98 61 535.2 0 61 99 108 535.2 0 108 100 254 535.2 0 254 101 175 535.2 0 175 102 89 535.2 0 89 103 58 535.2 0 58 104 57 535.2 0 57 105 76 535.2 0 76 106 55 535.2 0 55 107 70 535.2 0 70 108 55 535.2 0 55 109 66 535.2 0 66 110 66 535.2 0 66 111 65 535.2 0 65 112 59 535.2 0 59 113 79 535.2 0 79 114 76 535.2 0 76 115 80 535.2 0 80 116 92 535.2 0 92 117 189 535.2 0 189 118 132 535.2 0 132 119 85 535.2 0 85 120 75 535.2 0 75 121 72 535.2 0 72 122 77 535.2 0 77 123 66 535.2 0 66 124 49 535.2 0 49 125 77 535.2 0 77 126 66 535.2 0 66 127 69 535.2 0 69 128 54 535.2 0 54 129 57 535.2 0 57 130 64 535.2 0 64 131 109 535.2 0 109 132 90 535.2 0 90 133 103 535.2 0 103 134 59 535.2 0 59 135 67 535.2 0 67 136 99 535.2 0 99 137 436 535.2 0 436 138 1171 535.2 0 1171 139 803 535.2 0 803 140 740 535.2 0 740 141 58 535.2 0 58 142 64 535.2 0 64 143 85 535.2 0 85 144 110 535.2 0 110 145 87 535.2 0 87 146 75 535.2 0 75 147 67 535.2 0 67 148 62 535.2 0 62 149 67 535.2 0 67 150 74 535.2 0 74 151 69 535.2 0 69 152 67 535.2 0 67 153 49 535.2 0 49 154 54 535.2 0 54 155 62 535.2 0 62 156 87 535.2 0 87 157 80 535.2 0 80 158 73 535.2 0 73 159 72 535.2 0 72 160 69 535.2 0 69 161 73 535.2 0 73 162 59 535.2 0 59 163 61 535.2 0 61 164 59 535.2 0 59 165 81 535.2 0 81 166 64 535.2 0 64 167 55 535.2 0 55 168 70 535.2 0 70 169 72 535.2 0 72 170 73 535.2 0 73 171 104 535.2 0 104 172 102 535.2 0 102 173 91 535.2 0 91 174 56 535.2 0 56 175 68 535.2 0 68 176 63 535.2 0 63 177 60 535.2 0 60 178 87 535.2 0 87 179 81 535.2 0 81 180 70 535.2 0 70 181 109 535.2 0 109 182 80 535.2 0 80 183 65 535.2 0 65 184 81 535.2 0 81 185 90 535.2 0 90 186 79 535.2 0 79 187 83 535.2 0 83 188 75 535.2 0 75 189 86 535.2 0 86 190 59 535.2 0 59 191 81 535.2 0 81 192 72 535.2 0 72 193 77 535.2 0 77 194 65 535.2 0 65 195 72 535.2 0 72 196 60 535.2 0 60 197 83 535.2 0 83 198 86 535.2 0 86 199 75 535.2 0 75 200 67 535.2 0 67 201 60 535.2 0 60 202 64 535.2 0 64 203 89 535.2 0 89 204 93 535.2 0 93 205 84 535.2 0 84 206 77 535.2 0 77 207 81 535.2 0 81 208 75 535.2 0 75 209 73 535.2 0 73 210 108 535.2 0 108 211 106 535.2 0 106 212 85 535.2 0 85 213 82 535.2 0 82 214 126 535.2 0 126 215 125 535.2 0 125 216 131 535.2 0 131 217 87 535.2 0 87 218 97 535.2 0 97 219 65 535.2 0 65 220 92 535.2 0 92 221 69 535.2 0 69 222 80 535.2 0 80 223 87 535.2 0 87 224 120 535.2 0 120 225 103 535.2 0 103 226 70 535.2 0 70 227 182 535.2 0 182 228 97 535.2 0 97 229 98 535.2 0 98 230 88 535.2 0 88 231 80 535.2 0 80 232 64 535.2 0 64 233 90 535.2 0 90 234 65 535.2 0 65 235 57 535.2 0 57 236 54 535.2 0 54 237 82 535.2 0 82 238 142 535.2 0 142 239 166 535.2 0 166 240 173 535.2 0 173 241 68 535.2 0 68 242 72 535.2 0 72 243 98 535.2 0 98 244 70 535.2 0 70 245 80 535.2 0 80 246 76 535.2 0 76 247 77 535.2 0 77 248 82 535.2 0 82 249 71 535.2 0 71 250 64 535.2 0 64 251 105 535.2 0 105 252 101 535.2 0 101 253 97 535.2 0 97 254 95 535.2 0 95 255 79 535.2 0 79 256 126 535.2 0 126 257 175 535.2 0 175 258 265 535.2 0 265 259 214 535.2 0 214 260 176 535.2 0 176 261 97 535.2 0 97 262 87 535.2 0 87 263 111 535.2 0 111 264 128 535.2 0 128 265 99 535.2 0 99 266 134 535.2 0 134 267 96 535.2 0 96 268 95 535.2 0 95 269 86 535.2 0 86 270 144 535.2 0 144 271 196 535.2 0 196 272 279 535.2 0 279 273 1845 535.2 0 1845 274 3243 535.2 0 3243 275 2669 535.2 0 2669 276 225 535.2 0 225 277 434 535.2 0 434 278 322 535.2 0 322 279 191 535.2 0 191 280 122 535.2 0 122 281 97 535.2 0 97 282 127 535.2 0 127 283 260 535.2 0 260 284 342 535.2 0 342 285 736 535.2 0 736 286 1329 535.2 0 1329 287 587 535.2 0 587 288 958 535.2 0 958 289 162 535.2 0 162 290 140 535.2 0 140 291 272 535.2 0 272 292 300 535.2 0 300 293 499 535.2 0 499 294 495 535.2 0 495 295 351 535.2 0 351 296 219 535.2 0 219 297 384 535.2 0 384 298 1432 535.2 0 1432 299 3744 535.2 0 3744 300 2722 535.2 0 2722 301 1616 535.2 0 1616 Finished in 1.45 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 29,457 Reads with adapters: 6,776 (23.0%) Reads that were too short: 19 (0.1%) Reads written (passing filters): 6,757 (22.9%) Total basepairs processed: 6,846,242 bp Total written (filtered): 1,839,346 bp (26.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6776 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 6 0.0 3 0 0 3 3 19 80 0.0 3 0 62 5 10 3 20 4706 0.0 4 4230 356 91 22 7 21 34 0.0 4 5 22 2 4 1 22 1880 0.0 4 1731 120 21 6 2 23 37 0.0 4 6 5 2 15 9 25 4 0.0 4 0 0 0 3 1 28 1 0.0 4 0 1 35 2 0.0 4 0 0 0 0 2 53 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 3 0.0 4 0 0 0 0 3 144 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 156 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 168 1 0.0 4 1 185 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 2 0.0 4 0 0 0 0 2 267 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.25 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 6,757 Reads with adapters: 1,568 (23.2%) Reads written (passing filters): 6,757 (100.0%) Total basepairs processed: 1,839,346 bp Total written (filtered): 1,821,645 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1568 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.9% C: 9.2% G: 85.4% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 105.6 0 7 4 4 26.4 0 4 8 2 0.1 0 2 9 292 0.0 0 34 258 10 731 0.0 1 56 675 11 510 0.0 1 22 488 12 9 0.0 1 0 9 13 2 0.0 1 0 2 14 1 0.0 1 0 1 31 1 0.0 1 1 151 1 0.0 1 1 208 1 0.0 1 1 223 6 0.0 1 6 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.