This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.70 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 56,293 (2.6%) Reads that were too short: 32,133 (1.5%) Reads written (passing filters): 24,160 (1.1%) Total basepairs processed: 659,880,795 bp Total written (filtered): 5,948,217 bp (0.9%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 56293 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2398 535.2 0 2398 7 168 535.2 0 168 8 5073 535.2 0 5073 9 5475 535.2 0 5475 10 47 535.2 0 47 11 39 535.2 0 39 12 65 535.2 0 65 13 48 535.2 0 48 14 56 535.2 0 56 15 40 535.2 0 40 16 46 535.2 0 46 17 37 535.2 0 37 18 47 535.2 0 47 19 66 535.2 0 66 20 53 535.2 0 53 21 62 535.2 0 62 22 44 535.2 0 44 23 48 535.2 0 48 24 39 535.2 0 39 25 49 535.2 0 49 26 44 535.2 0 44 27 52 535.2 0 52 28 38 535.2 0 38 29 52 535.2 0 52 30 47 535.2 0 47 31 62 535.2 0 62 32 39 535.2 0 39 33 37 535.2 0 37 34 41 535.2 0 41 35 60 535.2 0 60 36 55 535.2 0 55 37 46 535.2 0 46 38 60 535.2 0 60 39 45 535.2 0 45 40 46 535.2 0 46 41 42 535.2 0 42 42 49 535.2 0 49 43 49 535.2 0 49 44 50 535.2 0 50 45 52 535.2 0 52 46 63 535.2 0 63 47 57 535.2 0 57 48 47 535.2 0 47 49 44 535.2 0 44 50 44 535.2 0 44 51 41 535.2 0 41 52 54 535.2 0 54 53 58 535.2 0 58 54 54 535.2 0 54 55 53 535.2 0 53 56 44 535.2 0 44 57 46 535.2 0 46 58 48 535.2 0 48 59 57 535.2 0 57 60 53 535.2 0 53 61 52 535.2 0 52 62 50 535.2 0 50 63 43 535.2 0 43 64 47 535.2 0 47 65 45 535.2 0 45 66 68 535.2 0 68 67 58 535.2 0 58 68 56 535.2 0 56 69 57 535.2 0 57 70 48 535.2 0 48 71 53 535.2 0 53 72 64 535.2 0 64 73 55 535.2 0 55 74 53 535.2 0 53 75 78 535.2 0 78 76 39 535.2 0 39 77 50 535.2 0 50 78 70 535.2 0 70 79 46 535.2 0 46 80 48 535.2 0 48 81 49 535.2 0 49 82 74 535.2 0 74 83 40 535.2 0 40 84 35 535.2 0 35 85 42 535.2 0 42 86 39 535.2 0 39 87 36 535.2 0 36 88 42 535.2 0 42 89 44 535.2 0 44 90 47 535.2 0 47 91 53 535.2 0 53 92 57 535.2 0 57 93 69 535.2 0 69 94 49 535.2 0 49 95 40 535.2 0 40 96 60 535.2 0 60 97 54 535.2 0 54 98 68 535.2 0 68 99 54 535.2 0 54 100 65 535.2 0 65 101 68 535.2 0 68 102 67 535.2 0 67 103 47 535.2 0 47 104 50 535.2 0 50 105 48 535.2 0 48 106 52 535.2 0 52 107 65 535.2 0 65 108 49 535.2 0 49 109 64 535.2 0 64 110 62 535.2 0 62 111 40 535.2 0 40 112 50 535.2 0 50 113 48 535.2 0 48 114 51 535.2 0 51 115 46 535.2 0 46 116 55 535.2 0 55 117 139 535.2 0 139 118 77 535.2 0 77 119 48 535.2 0 48 120 59 535.2 0 59 121 68 535.2 0 68 122 56 535.2 0 56 123 41 535.2 0 41 124 36 535.2 0 36 125 50 535.2 0 50 126 38 535.2 0 38 127 36 535.2 0 36 128 41 535.2 0 41 129 55 535.2 0 55 130 51 535.2 0 51 131 75 535.2 0 75 132 54 535.2 0 54 133 60 535.2 0 60 134 52 535.2 0 52 135 40 535.2 0 40 136 51 535.2 0 51 137 160 535.2 0 160 138 401 535.2 0 401 139 265 535.2 0 265 140 283 535.2 0 283 141 44 535.2 0 44 142 42 535.2 0 42 143 52 535.2 0 52 144 75 535.2 0 75 145 51 535.2 0 51 146 52 535.2 0 52 147 40 535.2 0 40 148 58 535.2 0 58 149 57 535.2 0 57 150 54 535.2 0 54 151 50 535.2 0 50 152 43 535.2 0 43 153 56 535.2 0 56 154 48 535.2 0 48 155 46 535.2 0 46 156 50 535.2 0 50 157 56 535.2 0 56 158 47 535.2 0 47 159 42 535.2 0 42 160 48 535.2 0 48 161 61 535.2 0 61 162 66 535.2 0 66 163 46 535.2 0 46 164 49 535.2 0 49 165 55 535.2 0 55 166 55 535.2 0 55 167 45 535.2 0 45 168 44 535.2 0 44 169 50 535.2 0 50 170 42 535.2 0 42 171 72 535.2 0 72 172 67 535.2 0 67 173 75 535.2 0 75 174 59 535.2 0 59 175 44 535.2 0 44 176 60 535.2 0 60 177 47 535.2 0 47 178 82 535.2 0 82 179 81 535.2 0 81 180 63 535.2 0 63 181 67 535.2 0 67 182 44 535.2 0 44 183 61 535.2 0 61 184 56 535.2 0 56 185 62 535.2 0 62 186 58 535.2 0 58 187 62 535.2 0 62 188 54 535.2 0 54 189 62 535.2 0 62 190 43 535.2 0 43 191 41 535.2 0 41 192 69 535.2 0 69 193 51 535.2 0 51 194 54 535.2 0 54 195 57 535.2 0 57 196 54 535.2 0 54 197 50 535.2 0 50 198 59 535.2 0 59 199 58 535.2 0 58 200 52 535.2 0 52 201 48 535.2 0 48 202 71 535.2 0 71 203 44 535.2 0 44 204 71 535.2 0 71 205 50 535.2 0 50 206 47 535.2 0 47 207 53 535.2 0 53 208 69 535.2 0 69 209 67 535.2 0 67 210 59 535.2 0 59 211 51 535.2 0 51 212 75 535.2 0 75 213 54 535.2 0 54 214 79 535.2 0 79 215 52 535.2 0 52 216 69 535.2 0 69 217 69 535.2 0 69 218 66 535.2 0 66 219 61 535.2 0 61 220 61 535.2 0 61 221 47 535.2 0 47 222 64 535.2 0 64 223 63 535.2 0 63 224 102 535.2 0 102 225 77 535.2 0 77 226 78 535.2 0 78 227 212 535.2 0 212 228 72 535.2 0 72 229 42 535.2 0 42 230 45 535.2 0 45 231 58 535.2 0 58 232 31 535.2 0 31 233 76 535.2 0 76 234 57 535.2 0 57 235 67 535.2 0 67 236 55 535.2 0 55 237 49 535.2 0 49 238 71 535.2 0 71 239 104 535.2 0 104 240 103 535.2 0 103 241 59 535.2 0 59 242 76 535.2 0 76 243 56 535.2 0 56 244 55 535.2 0 55 245 60 535.2 0 60 246 52 535.2 0 52 247 52 535.2 0 52 248 83 535.2 0 83 249 62 535.2 0 62 250 58 535.2 0 58 251 81 535.2 0 81 252 71 535.2 0 71 253 54 535.2 0 54 254 44 535.2 0 44 255 51 535.2 0 51 256 62 535.2 0 62 257 73 535.2 0 73 258 93 535.2 0 93 259 100 535.2 0 100 260 124 535.2 0 124 261 73 535.2 0 73 262 64 535.2 0 64 263 85 535.2 0 85 264 65 535.2 0 65 265 78 535.2 0 78 266 91 535.2 0 91 267 97 535.2 0 97 268 102 535.2 0 102 269 96 535.2 0 96 270 140 535.2 0 140 271 146 535.2 0 146 272 207 535.2 0 207 273 2132 535.2 0 2132 274 3789 535.2 0 3789 275 3203 535.2 0 3203 276 96 535.2 0 96 277 113 535.2 0 113 278 131 535.2 0 131 279 98 535.2 0 98 280 84 535.2 0 84 281 81 535.2 0 81 282 82 535.2 0 82 283 136 535.2 0 136 284 218 535.2 0 218 285 509 535.2 0 509 286 1068 535.2 0 1068 287 305 535.2 0 305 288 364 535.2 0 364 289 85 535.2 0 85 290 106 535.2 0 106 291 121 535.2 0 121 292 190 535.2 0 190 293 655 535.2 0 655 294 783 535.2 0 783 295 442 535.2 0 442 296 152 535.2 0 152 297 355 535.2 0 355 298 1231 535.2 0 1231 299 3970 535.2 0 3970 300 3572 535.2 0 3572 301 2811 535.2 0 2811 Finished in 1.08 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 24,160 Reads with adapters: 5,002 (20.7%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 4,995 (20.7%) Total basepairs processed: 5,948,217 bp Total written (filtered): 1,377,339 bp (23.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5002 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 30 0.0 3 0 29 1 17 4934 0.0 3 4856 78 18 11 0.0 3 2 9 19 5 0.0 3 5 20 5 0.0 3 1 0 0 4 35 2 0.0 3 0 0 2 38 1 0.0 3 0 0 0 1 55 3 0.0 3 0 0 0 3 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 206 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.16 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 4,995 Reads with adapters: 16 (0.3%) Reads written (passing filters): 4,995 (100.0%) Total basepairs processed: 1,377,339 bp Total written (filtered): 1,377,291 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 16 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 87.5% C: 6.2% G: 6.2% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 16 78.0 0 16