This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.33 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 60,081 (2.7%) Reads that were too short: 11,504 (0.5%) Reads written (passing filters): 48,577 (2.2%) Total basepairs processed: 659,880,795 bp Total written (filtered): 11,812,715 bp (1.8%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 60081 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 943 535.2 0 943 7 11703 535.2 0 11703 8 13484 535.2 0 13484 9 48 535.2 0 48 10 50 535.2 0 50 11 72 535.2 0 72 12 40 535.2 0 40 13 48 535.2 0 48 14 66 535.2 0 66 15 42 535.2 0 42 16 27 535.2 0 27 17 37 535.2 0 37 18 32 535.2 0 32 19 39 535.2 0 39 20 36 535.2 0 36 21 37 535.2 0 37 22 46 535.2 0 46 23 27 535.2 0 27 24 42 535.2 0 42 25 44 535.2 0 44 26 46 535.2 0 46 27 32 535.2 0 32 28 40 535.2 0 40 29 33 535.2 0 33 30 86 535.2 0 86 31 98 535.2 0 98 32 27 535.2 0 27 33 50 535.2 0 50 34 50 535.2 0 50 35 50 535.2 0 50 36 41 535.2 0 41 37 54 535.2 0 54 38 45 535.2 0 45 39 47 535.2 0 47 40 59 535.2 0 59 41 41 535.2 0 41 42 45 535.2 0 45 43 61 535.2 0 61 44 51 535.2 0 51 45 47 535.2 0 47 46 41 535.2 0 41 47 59 535.2 0 59 48 66 535.2 0 66 49 69 535.2 0 69 50 1125 535.2 0 1125 51 1216 535.2 0 1216 52 1796 535.2 0 1796 53 27 535.2 0 27 54 60 535.2 0 60 55 36 535.2 0 36 56 32 535.2 0 32 57 42 535.2 0 42 58 43 535.2 0 43 59 38 535.2 0 38 60 41 535.2 0 41 61 42 535.2 0 42 62 20 535.2 0 20 63 40 535.2 0 40 64 47 535.2 0 47 65 70 535.2 0 70 66 47 535.2 0 47 67 45 535.2 0 45 68 48 535.2 0 48 69 34 535.2 0 34 70 26 535.2 0 26 71 33 535.2 0 33 72 35 535.2 0 35 73 45 535.2 0 45 74 147 535.2 0 147 75 269 535.2 0 269 76 365 535.2 0 365 77 38 535.2 0 38 78 80 535.2 0 80 79 31 535.2 0 31 80 58 535.2 0 58 81 44 535.2 0 44 82 44 535.2 0 44 83 40 535.2 0 40 84 43 535.2 0 43 85 47 535.2 0 47 86 41 535.2 0 41 87 25 535.2 0 25 88 33 535.2 0 33 89 26 535.2 0 26 90 40 535.2 0 40 91 35 535.2 0 35 92 37 535.2 0 37 93 49 535.2 0 49 94 33 535.2 0 33 95 31 535.2 0 31 96 42 535.2 0 42 97 45 535.2 0 45 98 70 535.2 0 70 99 52 535.2 0 52 100 48 535.2 0 48 101 56 535.2 0 56 102 33 535.2 0 33 103 62 535.2 0 62 104 51 535.2 0 51 105 47 535.2 0 47 106 38 535.2 0 38 107 42 535.2 0 42 108 29 535.2 0 29 109 40 535.2 0 40 110 42 535.2 0 42 111 53 535.2 0 53 112 63 535.2 0 63 113 38 535.2 0 38 114 80 535.2 0 80 115 119 535.2 0 119 116 163 535.2 0 163 117 192 535.2 0 192 118 91 535.2 0 91 119 77 535.2 0 77 120 79 535.2 0 79 121 66 535.2 0 66 122 62 535.2 0 62 123 68 535.2 0 68 124 90 535.2 0 90 125 521 535.2 0 521 126 850 535.2 0 850 127 899 535.2 0 899 128 44 535.2 0 44 129 55 535.2 0 55 130 150 535.2 0 150 131 76 535.2 0 76 132 118 535.2 0 118 133 44 535.2 0 44 134 37 535.2 0 37 135 77 535.2 0 77 136 42 535.2 0 42 137 71 535.2 0 71 138 56 535.2 0 56 139 39 535.2 0 39 140 39 535.2 0 39 141 57 535.2 0 57 142 43 535.2 0 43 143 26 535.2 0 26 144 48 535.2 0 48 145 39 535.2 0 39 146 24 535.2 0 24 147 54 535.2 0 54 148 28 535.2 0 28 149 46 535.2 0 46 150 51 535.2 0 51 151 61 535.2 0 61 152 49 535.2 0 49 153 57 535.2 0 57 154 62 535.2 0 62 155 51 535.2 0 51 156 37 535.2 0 37 157 61 535.2 0 61 158 90 535.2 0 90 159 72 535.2 0 72 160 78 535.2 0 78 161 51 535.2 0 51 162 71 535.2 0 71 163 70 535.2 0 70 164 632 535.2 0 632 165 1556 535.2 0 1556 166 1703 535.2 0 1703 167 49 535.2 0 49 168 107 535.2 0 107 169 26 535.2 0 26 170 90 535.2 0 90 171 32 535.2 0 32 172 32 535.2 0 32 173 39 535.2 0 39 174 41 535.2 0 41 175 42 535.2 0 42 176 33 535.2 0 33 177 48 535.2 0 48 178 48 535.2 0 48 179 55 535.2 0 55 180 48 535.2 0 48 181 61 535.2 0 61 182 46 535.2 0 46 183 64 535.2 0 64 184 53 535.2 0 53 185 39 535.2 0 39 186 51 535.2 0 51 187 42 535.2 0 42 188 40 535.2 0 40 189 58 535.2 0 58 190 69 535.2 0 69 191 87 535.2 0 87 192 104 535.2 0 104 193 173 535.2 0 173 194 111 535.2 0 111 195 84 535.2 0 84 196 481 535.2 0 481 197 586 535.2 0 586 198 587 535.2 0 587 199 202 535.2 0 202 200 65 535.2 0 65 201 66 535.2 0 66 202 66 535.2 0 66 203 65 535.2 0 65 204 125 535.2 0 125 205 57 535.2 0 57 206 134 535.2 0 134 207 66 535.2 0 66 208 65 535.2 0 65 209 70 535.2 0 70 210 94 535.2 0 94 211 107 535.2 0 107 212 58 535.2 0 58 213 100 535.2 0 100 214 58 535.2 0 58 215 86 535.2 0 86 216 429 535.2 0 429 217 776 535.2 0 776 218 560 535.2 0 560 219 160 535.2 0 160 220 60 535.2 0 60 221 62 535.2 0 62 222 82 535.2 0 82 223 93 535.2 0 93 224 55 535.2 0 55 225 68 535.2 0 68 226 105 535.2 0 105 227 128 535.2 0 128 228 60 535.2 0 60 229 56 535.2 0 56 230 75 535.2 0 75 231 57 535.2 0 57 232 55 535.2 0 55 233 57 535.2 0 57 234 51 535.2 0 51 235 63 535.2 0 63 236 58 535.2 0 58 237 42 535.2 0 42 238 84 535.2 0 84 239 91 535.2 0 91 240 78 535.2 0 78 241 108 535.2 0 108 242 68 535.2 0 68 243 59 535.2 0 59 244 51 535.2 0 51 245 61 535.2 0 61 246 89 535.2 0 89 247 327 535.2 0 327 248 749 535.2 0 749 249 982 535.2 0 982 250 376 535.2 0 376 251 95 535.2 0 95 252 75 535.2 0 75 253 64 535.2 0 64 254 65 535.2 0 65 255 70 535.2 0 70 256 62 535.2 0 62 257 53 535.2 0 53 258 68 535.2 0 68 259 80 535.2 0 80 260 62 535.2 0 62 261 43 535.2 0 43 262 58 535.2 0 58 263 49 535.2 0 49 264 44 535.2 0 44 265 46 535.2 0 46 266 53 535.2 0 53 267 56 535.2 0 56 268 65 535.2 0 65 269 50 535.2 0 50 270 77 535.2 0 77 271 109 535.2 0 109 272 55 535.2 0 55 273 50 535.2 0 50 274 52 535.2 0 52 275 62 535.2 0 62 276 78 535.2 0 78 277 65 535.2 0 65 278 68 535.2 0 68 279 53 535.2 0 53 280 66 535.2 0 66 281 70 535.2 0 70 282 54 535.2 0 54 283 48 535.2 0 48 284 60 535.2 0 60 285 48 535.2 0 48 286 58 535.2 0 58 287 62 535.2 0 62 288 46 535.2 0 46 289 81 535.2 0 81 290 173 535.2 0 173 291 322 535.2 0 322 292 192 535.2 0 192 293 117 535.2 0 117 294 61 535.2 0 61 295 49 535.2 0 49 296 66 535.2 0 66 297 145 535.2 0 145 298 132 535.2 0 132 299 148 535.2 0 148 300 120 535.2 0 120 301 133 535.2 0 133 Finished in 2.16 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 48,577 Reads with adapters: 12,242 (25.2%) Reads that were too short: 30 (0.1%) Reads written (passing filters): 12,212 (25.1%) Total basepairs processed: 11,812,715 bp Total written (filtered): 3,368,277 bp (28.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12242 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 3 0.0 3 0 0 2 1 16 86 0.0 3 0 86 17 11994 0.0 3 11752 238 4 18 22 0.0 3 1 19 1 1 19 4 0.0 3 0 0 4 20 5 0.0 3 1 0 0 4 24 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 27 36 0.0 3 0 0 0 36 28 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 1 1 46 24 0.0 3 0 0 0 24 49 7 0.0 3 0 0 0 7 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 87 4 0.0 3 0 0 0 4 90 4 0.0 3 0 0 1 3 92 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 7 0.0 3 0 0 0 7 100 3 0.0 3 0 0 0 3 103 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 117 3 0.0 3 0 0 0 3 118 1 0.0 3 0 0 0 1 120 3 0.0 3 0 0 0 3 141 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.42 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 12,212 Reads with adapters: 80 (0.7%) Reads written (passing filters): 12,212 (100.0%) Total basepairs processed: 3,368,277 bp Total written (filtered): 3,368,034 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 80 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 57.5% C: 11.2% G: 30.0% T: 1.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 77 190.8 0 77 4 3 47.7 0 3