This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.14 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 144,865 (6.6%) Reads that were too short: 75,502 (3.4%) Reads written (passing filters): 69,363 (3.2%) Total basepairs processed: 659,880,795 bp Total written (filtered): 15,755,248 bp (2.4%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 144865 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 776 535.2 0 776 7 15305 535.2 0 15305 8 461 535.2 0 461 9 16037 535.2 0 16037 10 81 535.2 0 81 11 56 535.2 0 56 12 52 535.2 0 52 13 37 535.2 0 37 14 64 535.2 0 64 15 111 535.2 0 111 16 98 535.2 0 98 17 62 535.2 0 62 18 72 535.2 0 72 19 46 535.2 0 46 20 35 535.2 0 35 21 31 535.2 0 31 22 61 535.2 0 61 23 51 535.2 0 51 24 36 535.2 0 36 25 44 535.2 0 44 26 40 535.2 0 40 27 59 535.2 0 59 28 43 535.2 0 43 29 47 535.2 0 47 30 49 535.2 0 49 31 48 535.2 0 48 32 32 535.2 0 32 33 43 535.2 0 43 34 58 535.2 0 58 35 62 535.2 0 62 36 42 535.2 0 42 37 42 535.2 0 42 38 57 535.2 0 57 39 71 535.2 0 71 40 72 535.2 0 72 41 73 535.2 0 73 42 49 535.2 0 49 43 50 535.2 0 50 44 59 535.2 0 59 45 52 535.2 0 52 46 49 535.2 0 49 47 51 535.2 0 51 48 63 535.2 0 63 49 54 535.2 0 54 50 49 535.2 0 49 51 72 535.2 0 72 52 51 535.2 0 51 53 48 535.2 0 48 54 54 535.2 0 54 55 60 535.2 0 60 56 74 535.2 0 74 57 311 535.2 0 311 58 349 535.2 0 349 59 730 535.2 0 730 60 59 535.2 0 59 61 62 535.2 0 62 62 45 535.2 0 45 63 62 535.2 0 62 64 53 535.2 0 53 65 55 535.2 0 55 66 43 535.2 0 43 67 34 535.2 0 34 68 69 535.2 0 69 69 56 535.2 0 56 70 53 535.2 0 53 71 49 535.2 0 49 72 88 535.2 0 88 73 71 535.2 0 71 74 83 535.2 0 83 75 63 535.2 0 63 76 69 535.2 0 69 77 49 535.2 0 49 78 48 535.2 0 48 79 53 535.2 0 53 80 81 535.2 0 81 81 56 535.2 0 56 82 64 535.2 0 64 83 79 535.2 0 79 84 63 535.2 0 63 85 64 535.2 0 64 86 68 535.2 0 68 87 66 535.2 0 66 88 97 535.2 0 97 89 81 535.2 0 81 90 119 535.2 0 119 91 90 535.2 0 90 92 103 535.2 0 103 93 58 535.2 0 58 94 43 535.2 0 43 95 50 535.2 0 50 96 44 535.2 0 44 97 53 535.2 0 53 98 77 535.2 0 77 99 83 535.2 0 83 100 68 535.2 0 68 101 67 535.2 0 67 102 67 535.2 0 67 103 83 535.2 0 83 104 132 535.2 0 132 105 177 535.2 0 177 106 155 535.2 0 155 107 234 535.2 0 234 108 253 535.2 0 253 109 310 535.2 0 310 110 110 535.2 0 110 111 54 535.2 0 54 112 62 535.2 0 62 113 79 535.2 0 79 114 77 535.2 0 77 115 89 535.2 0 89 116 92 535.2 0 92 117 113 535.2 0 113 118 69 535.2 0 69 119 114 535.2 0 114 120 207 535.2 0 207 121 220 535.2 0 220 122 1948 535.2 0 1948 123 749 535.2 0 749 124 1109 535.2 0 1109 125 104 535.2 0 104 126 50 535.2 0 50 127 71 535.2 0 71 128 83 535.2 0 83 129 110 535.2 0 110 130 208 535.2 0 208 131 93 535.2 0 93 132 105 535.2 0 105 133 160 535.2 0 160 134 147 535.2 0 147 135 145 535.2 0 145 136 109 535.2 0 109 137 140 535.2 0 140 138 84 535.2 0 84 139 104 535.2 0 104 140 166 535.2 0 166 141 400 535.2 0 400 142 597 535.2 0 597 143 540 535.2 0 540 144 186 535.2 0 186 145 144 535.2 0 144 146 150 535.2 0 150 147 718 535.2 0 718 148 497 535.2 0 497 149 942 535.2 0 942 150 401 535.2 0 401 151 3262 535.2 0 3262 152 3050 535.2 0 3050 153 2397 535.2 0 2397 154 113 535.2 0 113 155 155 535.2 0 155 156 161 535.2 0 161 157 456 535.2 0 456 158 438 535.2 0 438 159 515 535.2 0 515 160 152 535.2 0 152 161 257 535.2 0 257 162 409 535.2 0 409 163 376 535.2 0 376 164 163 535.2 0 163 165 85 535.2 0 85 166 99 535.2 0 99 167 124 535.2 0 124 168 108 535.2 0 108 169 135 535.2 0 135 170 153 535.2 0 153 171 93 535.2 0 93 172 73 535.2 0 73 173 44 535.2 0 44 174 50 535.2 0 50 175 104 535.2 0 104 176 162 535.2 0 162 177 183 535.2 0 183 178 128 535.2 0 128 179 96 535.2 0 96 180 65 535.2 0 65 181 107 535.2 0 107 182 267 535.2 0 267 183 298 535.2 0 298 184 240 535.2 0 240 185 90 535.2 0 90 186 160 535.2 0 160 187 254 535.2 0 254 188 196 535.2 0 196 189 245 535.2 0 245 190 109 535.2 0 109 191 70 535.2 0 70 192 89 535.2 0 89 193 96 535.2 0 96 194 91 535.2 0 91 195 210 535.2 0 210 196 268 535.2 0 268 197 197 535.2 0 197 198 151 535.2 0 151 199 77 535.2 0 77 200 100 535.2 0 100 201 168 535.2 0 168 202 146 535.2 0 146 203 132 535.2 0 132 204 143 535.2 0 143 205 162 535.2 0 162 206 216 535.2 0 216 207 303 535.2 0 303 208 474 535.2 0 474 209 1784 535.2 0 1784 210 1522 535.2 0 1522 211 1622 535.2 0 1622 212 1402 535.2 0 1402 213 9341 535.2 0 9341 214 12418 535.2 0 12418 215 15314 535.2 0 15314 216 751 535.2 0 751 217 183 535.2 0 183 218 176 535.2 0 176 219 244 535.2 0 244 220 491 535.2 0 491 221 3130 535.2 0 3130 222 3685 535.2 0 3685 223 3642 535.2 0 3642 224 1725 535.2 0 1725 225 112 535.2 0 112 226 96 535.2 0 96 227 153 535.2 0 153 228 115 535.2 0 115 229 70 535.2 0 70 230 93 535.2 0 93 231 186 535.2 0 186 232 292 535.2 0 292 233 262 535.2 0 262 234 147 535.2 0 147 235 127 535.2 0 127 236 125 535.2 0 125 237 173 535.2 0 173 238 140 535.2 0 140 239 128 535.2 0 128 240 113 535.2 0 113 241 107 535.2 0 107 242 139 535.2 0 139 243 268 535.2 0 268 244 322 535.2 0 322 245 346 535.2 0 346 246 188 535.2 0 188 247 71 535.2 0 71 248 65 535.2 0 65 249 83 535.2 0 83 250 107 535.2 0 107 251 88 535.2 0 88 252 105 535.2 0 105 253 118 535.2 0 118 254 145 535.2 0 145 255 177 535.2 0 177 256 219 535.2 0 219 257 604 535.2 0 604 258 689 535.2 0 689 259 806 535.2 0 806 260 385 535.2 0 385 261 157 535.2 0 157 262 225 535.2 0 225 263 407 535.2 0 407 264 421 535.2 0 421 265 452 535.2 0 452 266 209 535.2 0 209 267 113 535.2 0 113 268 157 535.2 0 157 269 274 535.2 0 274 270 385 535.2 0 385 271 329 535.2 0 329 272 233 535.2 0 233 273 155 535.2 0 155 274 121 535.2 0 121 275 157 535.2 0 157 276 185 535.2 0 185 277 199 535.2 0 199 278 145 535.2 0 145 279 135 535.2 0 135 280 133 535.2 0 133 281 311 535.2 0 311 282 507 535.2 0 507 283 477 535.2 0 477 284 318 535.2 0 318 285 270 535.2 0 270 286 268 535.2 0 268 287 116 535.2 0 116 288 86 535.2 0 86 289 189 535.2 0 189 290 332 535.2 0 332 291 314 535.2 0 314 292 132 535.2 0 132 293 95 535.2 0 95 294 112 535.2 0 112 295 127 535.2 0 127 296 197 535.2 0 197 297 201 535.2 0 201 298 188 535.2 0 188 299 124 535.2 0 124 300 110 535.2 0 110 301 66 535.2 0 66 Finished in 3.27 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 69,363 Reads with adapters: 15,485 (22.3%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 15,451 (22.3%) Total basepairs processed: 15,755,248 bp Total written (filtered): 4,217,379 bp (26.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15485 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 10 0.0 3 0 0 10 19 194 0.0 3 0 151 26 14 3 20 15010 0.0 4 13991 802 153 48 16 21 46 0.0 4 6 35 4 0 1 22 4 0.0 4 3 0 1 23 39 0.0 4 2 0 0 26 11 26 3 0.0 4 2 0 0 0 1 27 17 0.0 4 0 0 0 0 17 28 4 0.0 4 0 0 0 0 4 30 2 0.0 4 0 0 0 0 2 33 1 0.0 4 1 54 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 126 1 0.0 4 0 0 0 0 1 127 112 0.0 4 0 0 0 0 112 129 1 0.0 4 0 0 0 0 1 130 3 0.0 4 0 0 0 0 3 148 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 0 2 212 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 1 228 4 0.0 4 0 0 0 0 4 229 7 0.0 4 0 0 0 0 7 234 1 0.0 4 0 0 0 1 236 1 0.0 4 0 0 0 0 1 259 2 0.0 4 0 0 0 2 265 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.53 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 15,451 Reads with adapters: 1,172 (7.6%) Reads written (passing filters): 15,451 (100.0%) Total basepairs processed: 4,217,379 bp Total written (filtered): 4,201,365 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1172 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.0% C: 13.4% G: 76.9% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 78 241.4 0 78 4 33 60.4 0 33 7 1 0.9 0 1 8 1 0.2 0 1 9 4 0.1 0 0 4 10 18 0.0 1 1 17 11 780 0.0 1 34 746 12 222 0.0 1 1 221 13 12 0.0 1 0 12 14 1 0.0 1 0 1 15 1 0.0 1 0 1 16 1 0.0 1 0 1 123 2 0.0 1 2 152 5 0.0 1 2 3 191 1 0.0 1 0 1 210 7 0.0 1 4 3 261 5 0.0 1 4 1