This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.34 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 127,229 (5.8%) Reads that were too short: 64,669 (2.9%) Reads written (passing filters): 62,560 (2.9%) Total basepairs processed: 659,880,795 bp Total written (filtered): 14,885,509 bp (2.3%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 127229 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 678 535.2 0 678 7 18314 535.2 0 18314 8 206 535.2 0 206 9 15184 535.2 0 15184 10 63 535.2 0 63 11 36 535.2 0 36 12 41 535.2 0 41 13 31 535.2 0 31 14 43 535.2 0 43 15 51 535.2 0 51 16 30 535.2 0 30 17 57 535.2 0 57 18 43 535.2 0 43 19 48 535.2 0 48 20 27 535.2 0 27 21 22 535.2 0 22 22 37 535.2 0 37 23 62 535.2 0 62 24 45 535.2 0 45 25 38 535.2 0 38 26 33 535.2 0 33 27 112 535.2 0 112 28 302 535.2 0 302 29 30 535.2 0 30 30 30 535.2 0 30 31 37 535.2 0 37 32 40 535.2 0 40 33 35 535.2 0 35 34 34 535.2 0 34 35 44 535.2 0 44 36 35 535.2 0 35 37 41 535.2 0 41 38 23 535.2 0 23 39 35 535.2 0 35 40 30 535.2 0 30 41 84 535.2 0 84 42 30 535.2 0 30 43 34 535.2 0 34 44 30 535.2 0 30 45 45 535.2 0 45 46 40 535.2 0 40 47 43 535.2 0 43 48 43 535.2 0 43 49 38 535.2 0 38 50 36 535.2 0 36 51 47 535.2 0 47 52 46 535.2 0 46 53 37 535.2 0 37 54 55 535.2 0 55 55 51 535.2 0 51 56 62 535.2 0 62 57 327 535.2 0 327 58 360 535.2 0 360 59 1014 535.2 0 1014 60 30 535.2 0 30 61 39 535.2 0 39 62 44 535.2 0 44 63 39 535.2 0 39 64 40 535.2 0 40 65 38 535.2 0 38 66 40 535.2 0 40 67 37 535.2 0 37 68 40 535.2 0 40 69 48 535.2 0 48 70 26 535.2 0 26 71 43 535.2 0 43 72 35 535.2 0 35 73 44 535.2 0 44 74 43 535.2 0 43 75 23 535.2 0 23 76 37 535.2 0 37 77 56 535.2 0 56 78 42 535.2 0 42 79 53 535.2 0 53 80 66 535.2 0 66 81 60 535.2 0 60 82 44 535.2 0 44 83 70 535.2 0 70 84 49 535.2 0 49 85 58 535.2 0 58 86 69 535.2 0 69 87 63 535.2 0 63 88 150 535.2 0 150 89 89 535.2 0 89 90 157 535.2 0 157 91 97 535.2 0 97 92 80 535.2 0 80 93 67 535.2 0 67 94 39 535.2 0 39 95 39 535.2 0 39 96 44 535.2 0 44 97 53 535.2 0 53 98 55 535.2 0 55 99 57 535.2 0 57 100 52 535.2 0 52 101 56 535.2 0 56 102 69 535.2 0 69 103 56 535.2 0 56 104 79 535.2 0 79 105 100 535.2 0 100 106 94 535.2 0 94 107 127 535.2 0 127 108 81 535.2 0 81 109 95 535.2 0 95 110 68 535.2 0 68 111 56 535.2 0 56 112 42 535.2 0 42 113 58 535.2 0 58 114 72 535.2 0 72 115 74 535.2 0 74 116 52 535.2 0 52 117 88 535.2 0 88 118 51 535.2 0 51 119 68 535.2 0 68 120 72 535.2 0 72 121 88 535.2 0 88 122 593 535.2 0 593 123 352 535.2 0 352 124 477 535.2 0 477 125 53 535.2 0 53 126 55 535.2 0 55 127 74 535.2 0 74 128 43 535.2 0 43 129 69 535.2 0 69 130 165 535.2 0 165 131 77 535.2 0 77 132 73 535.2 0 73 133 125 535.2 0 125 134 121 535.2 0 121 135 103 535.2 0 103 136 57 535.2 0 57 137 118 535.2 0 118 138 60 535.2 0 60 139 73 535.2 0 73 140 119 535.2 0 119 141 249 535.2 0 249 142 269 535.2 0 269 143 180 535.2 0 180 144 85 535.2 0 85 145 74 535.2 0 74 146 119 535.2 0 119 147 425 535.2 0 425 148 237 535.2 0 237 149 526 535.2 0 526 150 369 535.2 0 369 151 3693 535.2 0 3693 152 3240 535.2 0 3240 153 2837 535.2 0 2837 154 78 535.2 0 78 155 120 535.2 0 120 156 94 535.2 0 94 157 349 535.2 0 349 158 307 535.2 0 307 159 375 535.2 0 375 160 85 535.2 0 85 161 127 535.2 0 127 162 177 535.2 0 177 163 185 535.2 0 185 164 79 535.2 0 79 165 57 535.2 0 57 166 51 535.2 0 51 167 63 535.2 0 63 168 81 535.2 0 81 169 82 535.2 0 82 170 117 535.2 0 117 171 73 535.2 0 73 172 47 535.2 0 47 173 44 535.2 0 44 174 51 535.2 0 51 175 74 535.2 0 74 176 81 535.2 0 81 177 106 535.2 0 106 178 63 535.2 0 63 179 59 535.2 0 59 180 62 535.2 0 62 181 61 535.2 0 61 182 154 535.2 0 154 183 134 535.2 0 134 184 155 535.2 0 155 185 67 535.2 0 67 186 124 535.2 0 124 187 119 535.2 0 119 188 113 535.2 0 113 189 107 535.2 0 107 190 73 535.2 0 73 191 59 535.2 0 59 192 61 535.2 0 61 193 73 535.2 0 73 194 75 535.2 0 75 195 124 535.2 0 124 196 197 535.2 0 197 197 105 535.2 0 105 198 98 535.2 0 98 199 64 535.2 0 64 200 102 535.2 0 102 201 158 535.2 0 158 202 107 535.2 0 107 203 97 535.2 0 97 204 90 535.2 0 90 205 100 535.2 0 100 206 151 535.2 0 151 207 176 535.2 0 176 208 307 535.2 0 307 209 654 535.2 0 654 210 689 535.2 0 689 211 904 535.2 0 904 212 1211 535.2 0 1211 213 11414 535.2 0 11414 214 14687 535.2 0 14687 215 19751 535.2 0 19751 216 818 535.2 0 818 217 115 535.2 0 115 218 94 535.2 0 94 219 103 535.2 0 103 220 256 535.2 0 256 221 1123 535.2 0 1123 222 1205 535.2 0 1205 223 1203 535.2 0 1203 224 607 535.2 0 607 225 86 535.2 0 86 226 75 535.2 0 75 227 129 535.2 0 129 228 73 535.2 0 73 229 60 535.2 0 60 230 57 535.2 0 57 231 111 535.2 0 111 232 111 535.2 0 111 233 105 535.2 0 105 234 94 535.2 0 94 235 123 535.2 0 123 236 97 535.2 0 97 237 163 535.2 0 163 238 92 535.2 0 92 239 70 535.2 0 70 240 76 535.2 0 76 241 70 535.2 0 70 242 97 535.2 0 97 243 131 535.2 0 131 244 121 535.2 0 121 245 97 535.2 0 97 246 94 535.2 0 94 247 52 535.2 0 52 248 59 535.2 0 59 249 60 535.2 0 60 250 62 535.2 0 62 251 79 535.2 0 79 252 62 535.2 0 62 253 57 535.2 0 57 254 81 535.2 0 81 255 92 535.2 0 92 256 78 535.2 0 78 257 241 535.2 0 241 258 250 535.2 0 250 259 327 535.2 0 327 260 133 535.2 0 133 261 93 535.2 0 93 262 127 535.2 0 127 263 147 535.2 0 147 264 182 535.2 0 182 265 183 535.2 0 183 266 94 535.2 0 94 267 64 535.2 0 64 268 88 535.2 0 88 269 117 535.2 0 117 270 153 535.2 0 153 271 151 535.2 0 151 272 121 535.2 0 121 273 165 535.2 0 165 274 101 535.2 0 101 275 126 535.2 0 126 276 119 535.2 0 119 277 131 535.2 0 131 278 117 535.2 0 117 279 113 535.2 0 113 280 86 535.2 0 86 281 169 535.2 0 169 282 331 535.2 0 331 283 295 535.2 0 295 284 118 535.2 0 118 285 100 535.2 0 100 286 92 535.2 0 92 287 84 535.2 0 84 288 64 535.2 0 64 289 110 535.2 0 110 290 143 535.2 0 143 291 165 535.2 0 165 292 95 535.2 0 95 293 63 535.2 0 63 294 98 535.2 0 98 295 90 535.2 0 90 296 124 535.2 0 124 297 118 535.2 0 118 298 103 535.2 0 103 299 87 535.2 0 87 300 82 535.2 0 82 301 33 535.2 0 33 Finished in 2.76 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 62,560 Reads with adapters: 15,830 (25.3%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 15,806 (25.3%) Total basepairs processed: 14,885,509 bp Total written (filtered): 4,340,401 bp (29.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15830 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 6 0.0 3 0 0 6 16 128 0.0 3 0 126 1 1 17 15306 0.0 3 15029 270 7 18 29 0.0 3 1 26 2 19 22 0.0 3 3 1 15 3 20 273 0.0 3 255 7 1 10 32 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 38 1 0.0 3 1 46 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 1 78 2 0.0 3 0 0 1 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 160 29 0.0 3 0 0 0 29 161 2 0.0 3 0 0 0 2 162 2 0.0 3 0 0 0 2 164 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.57 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 15,806 Reads with adapters: 1,493 (9.4%) Reads written (passing filters): 15,806 (100.0%) Total basepairs processed: 4,340,401 bp Total written (filtered): 4,335,899 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1493 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 87.3% C: 6.8% G: 5.2% T: 0.7% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1475 247.0 0 1475 4 17 61.7 0 17 9 1 0.1 0 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.