This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 60.14 s (27 us/read; 2.19 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 436,755 (19.9%) Reads that were too short: 19,729 (0.9%) Reads written (passing filters): 417,026 (19.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 92,157,182 bp (14.0%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 436755 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 312 535.2 0 312 7 658 535.2 0 658 8 8909 535.2 0 8909 9 6876 535.2 0 6876 10 94 535.2 0 94 11 101 535.2 0 101 12 119 535.2 0 119 13 106 535.2 0 106 14 96 535.2 0 96 15 121 535.2 0 121 16 78 535.2 0 78 17 94 535.2 0 94 18 132 535.2 0 132 19 148 535.2 0 148 20 137 535.2 0 137 21 101 535.2 0 101 22 166 535.2 0 166 23 314 535.2 0 314 24 275 535.2 0 275 25 126 535.2 0 126 26 118 535.2 0 118 27 149 535.2 0 149 28 102 535.2 0 102 29 123 535.2 0 123 30 160 535.2 0 160 31 119 535.2 0 119 32 147 535.2 0 147 33 132 535.2 0 132 34 152 535.2 0 152 35 144 535.2 0 144 36 269 535.2 0 269 37 218 535.2 0 218 38 493 535.2 0 493 39 246 535.2 0 246 40 195 535.2 0 195 41 117 535.2 0 117 42 244 535.2 0 244 43 158 535.2 0 158 44 208 535.2 0 208 45 337 535.2 0 337 46 313 535.2 0 313 47 430 535.2 0 430 48 767 535.2 0 767 49 1734 535.2 0 1734 50 454 535.2 0 454 51 625 535.2 0 625 52 1088 535.2 0 1088 53 739 535.2 0 739 54 675 535.2 0 675 55 1052 535.2 0 1052 56 1183 535.2 0 1183 57 1434 535.2 0 1434 58 1718 535.2 0 1718 59 2766 535.2 0 2766 60 2681 535.2 0 2681 61 3967 535.2 0 3967 62 63867 535.2 0 63867 63 113385 535.2 0 113385 64 97943 535.2 0 97943 65 686 535.2 0 686 66 137 535.2 0 137 67 95 535.2 0 95 68 103 535.2 0 103 69 122 535.2 0 122 70 164 535.2 0 164 71 717 535.2 0 717 72 835 535.2 0 835 73 848 535.2 0 848 74 237 535.2 0 237 75 211 535.2 0 211 76 2192 535.2 0 2192 77 1401 535.2 0 1401 78 1073 535.2 0 1073 79 177 535.2 0 177 80 90 535.2 0 90 81 92 535.2 0 92 82 104 535.2 0 104 83 108 535.2 0 108 84 93 535.2 0 93 85 93 535.2 0 93 86 99 535.2 0 99 87 106 535.2 0 106 88 85 535.2 0 85 89 69 535.2 0 69 90 96 535.2 0 96 91 114 535.2 0 114 92 96 535.2 0 96 93 164 535.2 0 164 94 122 535.2 0 122 95 106 535.2 0 106 96 58 535.2 0 58 97 76 535.2 0 76 98 87 535.2 0 87 99 110 535.2 0 110 100 118 535.2 0 118 101 98 535.2 0 98 102 139 535.2 0 139 103 181 535.2 0 181 104 184 535.2 0 184 105 171 535.2 0 171 106 229 535.2 0 229 107 1150 535.2 0 1150 108 788 535.2 0 788 109 1500 535.2 0 1500 110 152 535.2 0 152 111 226 535.2 0 226 112 214 535.2 0 214 113 89 535.2 0 89 114 91 535.2 0 91 115 82 535.2 0 82 116 106 535.2 0 106 117 114 535.2 0 114 118 109 535.2 0 109 119 87 535.2 0 87 120 87 535.2 0 87 121 97 535.2 0 97 122 69 535.2 0 69 123 67 535.2 0 67 124 93 535.2 0 93 125 127 535.2 0 127 126 167 535.2 0 167 127 210 535.2 0 210 128 225 535.2 0 225 129 144 535.2 0 144 130 126 535.2 0 126 131 143 535.2 0 143 132 168 535.2 0 168 133 146 535.2 0 146 134 146 535.2 0 146 135 110 535.2 0 110 136 127 535.2 0 127 137 190 535.2 0 190 138 127 535.2 0 127 139 170 535.2 0 170 140 253 535.2 0 253 141 237 535.2 0 237 142 497 535.2 0 497 143 720 535.2 0 720 144 7672 535.2 0 7672 145 5071 535.2 0 5071 146 5618 535.2 0 5618 147 178 535.2 0 178 148 129 535.2 0 129 149 167 535.2 0 167 150 161 535.2 0 161 151 166 535.2 0 166 152 187 535.2 0 187 153 176 535.2 0 176 154 276 535.2 0 276 155 190 535.2 0 190 156 1696 535.2 0 1696 157 821 535.2 0 821 158 1342 535.2 0 1342 159 290 535.2 0 290 160 316 535.2 0 316 161 378 535.2 0 378 162 615 535.2 0 615 163 1297 535.2 0 1297 164 13081 535.2 0 13081 165 10988 535.2 0 10988 166 8713 535.2 0 8713 167 224 535.2 0 224 168 250 535.2 0 250 169 732 535.2 0 732 170 1384 535.2 0 1384 171 2347 535.2 0 2347 172 1598 535.2 0 1598 173 528 535.2 0 528 174 692 535.2 0 692 175 233 535.2 0 233 176 89 535.2 0 89 177 104 535.2 0 104 178 97 535.2 0 97 179 334 535.2 0 334 180 282 535.2 0 282 181 359 535.2 0 359 182 164 535.2 0 164 183 175 535.2 0 175 184 362 535.2 0 362 185 328 535.2 0 328 186 335 535.2 0 335 187 170 535.2 0 170 188 161 535.2 0 161 189 641 535.2 0 641 190 1775 535.2 0 1775 191 1440 535.2 0 1440 192 587 535.2 0 587 193 111 535.2 0 111 194 101 535.2 0 101 195 88 535.2 0 88 196 104 535.2 0 104 197 221 535.2 0 221 198 244 535.2 0 244 199 178 535.2 0 178 200 114 535.2 0 114 201 91 535.2 0 91 202 99 535.2 0 99 203 177 535.2 0 177 204 159 535.2 0 159 205 140 535.2 0 140 206 129 535.2 0 129 207 130 535.2 0 130 208 161 535.2 0 161 209 99 535.2 0 99 210 95 535.2 0 95 211 91 535.2 0 91 212 127 535.2 0 127 213 155 535.2 0 155 214 179 535.2 0 179 215 202 535.2 0 202 216 309 535.2 0 309 217 346 535.2 0 346 218 296 535.2 0 296 219 121 535.2 0 121 220 89 535.2 0 89 221 74 535.2 0 74 222 89 535.2 0 89 223 89 535.2 0 89 224 128 535.2 0 128 225 154 535.2 0 154 226 219 535.2 0 219 227 298 535.2 0 298 228 265 535.2 0 265 229 309 535.2 0 309 230 188 535.2 0 188 231 197 535.2 0 197 232 105 535.2 0 105 233 106 535.2 0 106 234 102 535.2 0 102 235 113 535.2 0 113 236 157 535.2 0 157 237 173 535.2 0 173 238 233 535.2 0 233 239 137 535.2 0 137 240 166 535.2 0 166 241 126 535.2 0 126 242 125 535.2 0 125 243 127 535.2 0 127 244 148 535.2 0 148 245 184 535.2 0 184 246 141 535.2 0 141 247 196 535.2 0 196 248 189 535.2 0 189 249 151 535.2 0 151 250 91 535.2 0 91 251 120 535.2 0 120 252 148 535.2 0 148 253 117 535.2 0 117 254 104 535.2 0 104 255 76 535.2 0 76 256 95 535.2 0 95 257 87 535.2 0 87 258 123 535.2 0 123 259 170 535.2 0 170 260 350 535.2 0 350 261 386 535.2 0 386 262 379 535.2 0 379 263 164 535.2 0 164 264 153 535.2 0 153 265 202 535.2 0 202 266 252 535.2 0 252 267 252 535.2 0 252 268 201 535.2 0 201 269 111 535.2 0 111 270 141 535.2 0 141 271 176 535.2 0 176 272 233 535.2 0 233 273 184 535.2 0 184 274 131 535.2 0 131 275 134 535.2 0 134 276 132 535.2 0 132 277 584 535.2 0 584 278 357 535.2 0 357 279 326 535.2 0 326 280 198 535.2 0 198 281 160 535.2 0 160 282 134 535.2 0 134 283 186 535.2 0 186 284 146 535.2 0 146 285 193 535.2 0 193 286 307 535.2 0 307 287 898 535.2 0 898 288 782 535.2 0 782 289 771 535.2 0 771 290 317 535.2 0 317 291 174 535.2 0 174 292 165 535.2 0 165 293 201 535.2 0 201 294 162 535.2 0 162 295 139 535.2 0 139 296 157 535.2 0 157 297 199 535.2 0 199 298 233 535.2 0 233 299 176 535.2 0 176 300 109 535.2 0 109 301 150 535.2 0 150 Finished in 17.72 s (42 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 417,026 Reads with adapters: 14,469 (3.5%) Reads that were too short: 347 (0.1%) Reads written (passing filters): 14,122 (3.4%) Total basepairs processed: 92,157,182 bp Total written (filtered): 3,142,994 bp (3.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14469 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 7 0.0 3 0 0 4 2 1 19 121 0.0 3 0 99 10 9 3 20 7296 0.0 4 6692 451 113 31 9 21 21 0.0 4 1 13 3 3 1 22 4 0.0 4 0 1 2 0 1 23 40 0.0 4 0 0 0 27 13 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 0 1 34 8 0.0 4 0 0 0 0 8 35 1 0.0 4 0 0 0 1 36 1 0.0 4 0 0 0 0 1 37 8 0.0 4 0 0 0 0 8 38 2 0.0 4 0 0 0 0 2 46 1 0.0 4 0 0 0 1 51 1 0.0 4 0 0 0 0 1 52 3 0.0 4 0 0 0 0 3 53 1 0.0 4 0 0 0 1 59 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 1 64 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 70 2 0.0 4 0 0 0 0 2 71 5 0.0 4 0 0 0 0 5 72 6525 0.0 4 0 0 0 1 6524 73 1 0.0 4 0 0 0 0 1 74 7 0.0 4 0 0 0 0 7 75 19 0.0 4 0 0 0 1 18 78 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 1 83 1 0.0 4 0 0 0 0 1 85 11 0.0 4 1 0 0 0 10 95 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 1 127 26 0.0 4 0 0 0 1 25 140 3 0.0 4 0 1 0 0 2 141 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 1 148 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 153 6 0.0 4 0 0 0 0 6 154 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 1 160 1 0.0 4 0 0 0 0 1 161 2 0.0 4 0 0 0 0 2 163 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 1 166 4 0.0 4 0 0 0 0 4 167 2 0.0 4 0 0 0 0 2 168 4 0.0 4 0 0 0 0 4 170 8 0.0 4 0 0 0 0 8 171 1 0.0 4 0 0 0 0 1 172 2 0.0 4 0 0 0 0 2 173 60 0.0 4 0 0 0 1 59 174 17 0.0 4 0 0 0 0 17 175 4 0.0 4 0 0 0 1 3 176 2 0.0 4 0 0 0 0 2 177 2 0.0 4 0 0 0 1 1 178 10 0.0 4 0 0 0 0 10 179 18 0.0 4 0 0 0 1 17 180 16 0.0 4 0 0 0 0 16 181 10 0.0 4 0 0 0 0 10 182 2 0.0 4 0 0 0 0 2 183 1 0.0 4 0 0 0 0 1 187 2 0.0 4 0 0 0 0 2 190 2 0.0 4 0 0 0 0 2 198 1 0.0 4 0 0 0 0 1 199 2 0.0 4 0 0 0 0 2 200 1 0.0 4 0 0 0 0 1 201 3 0.0 4 0 0 0 0 3 202 6 0.0 4 0 0 0 0 6 203 3 0.0 4 0 0 0 0 3 204 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 1 1 220 2 0.0 4 0 0 0 1 1 226 1 0.0 4 0 0 0 0 1 227 50 0.0 4 0 0 0 2 48 228 22 0.0 4 0 0 0 2 20 229 5 0.0 4 0 0 0 0 5 230 7 0.0 4 0 0 0 1 6 233 20 0.0 4 0 0 0 0 20 234 3 0.0 4 0 0 0 0 3 235 3 0.0 4 0 0 0 1 2 236 1 0.0 4 0 0 0 0 1 237 4 0.0 4 0 0 0 0 4 240 2 0.0 4 0 0 0 1 1 241 3 0.0 4 0 0 0 0 3 281 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.45 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 14,122 Reads with adapters: 2,133 (15.1%) Reads written (passing filters): 14,122 (100.0%) Total basepairs processed: 3,142,994 bp Total written (filtered): 3,121,131 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2133 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.8% C: 8.7% G: 82.6% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 49 220.7 0 49 4 56 55.2 0 56 5 1 13.8 0 1 7 1 0.9 0 1 8 2 0.2 0 2 9 711 0.1 0 81 630 10 1019 0.0 1 65 954 11 268 0.0 1 10 258 12 10 0.0 1 0 10 13 6 0.0 1 0 6 14 1 0.0 1 0 1 150 3 0.0 1 2 1 208 4 0.0 1 3 1 209 1 0.0 1 0 1 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.