This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 59.69 s (27 us/read; 2.20 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 406,459 (18.5%) Reads that were too short: 10,324 (0.5%) Reads written (passing filters): 396,135 (18.1%) Total basepairs processed: 659,880,795 bp Total written (filtered): 88,631,282 bp (13.4%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 406459 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 289 535.2 0 289 7 478 535.2 0 478 8 8234 535.2 0 8234 9 8782 535.2 0 8782 10 83 535.2 0 83 11 83 535.2 0 83 12 78 535.2 0 78 13 72 535.2 0 72 14 77 535.2 0 77 15 60 535.2 0 60 16 58 535.2 0 58 17 68 535.2 0 68 18 87 535.2 0 87 19 108 535.2 0 108 20 104 535.2 0 104 21 84 535.2 0 84 22 97 535.2 0 97 23 100 535.2 0 100 24 138 535.2 0 138 25 61 535.2 0 61 26 82 535.2 0 82 27 102 535.2 0 102 28 64 535.2 0 64 29 96 535.2 0 96 30 135 535.2 0 135 31 84 535.2 0 84 32 123 535.2 0 123 33 82 535.2 0 82 34 115 535.2 0 115 35 107 535.2 0 107 36 226 535.2 0 226 37 202 535.2 0 202 38 479 535.2 0 479 39 211 535.2 0 211 40 135 535.2 0 135 41 91 535.2 0 91 42 208 535.2 0 208 43 111 535.2 0 111 44 190 535.2 0 190 45 263 535.2 0 263 46 250 535.2 0 250 47 346 535.2 0 346 48 760 535.2 0 760 49 1614 535.2 0 1614 50 420 535.2 0 420 51 515 535.2 0 515 52 987 535.2 0 987 53 488 535.2 0 488 54 494 535.2 0 494 55 1059 535.2 0 1059 56 1147 535.2 0 1147 57 1263 535.2 0 1263 58 1370 535.2 0 1370 59 2433 535.2 0 2433 60 2206 535.2 0 2206 61 3118 535.2 0 3118 62 61624 535.2 0 61624 63 116552 535.2 0 116552 64 97426 535.2 0 97426 65 578 535.2 0 578 66 76 535.2 0 76 67 51 535.2 0 51 68 49 535.2 0 49 69 68 535.2 0 68 70 85 535.2 0 85 71 273 535.2 0 273 72 309 535.2 0 309 73 424 535.2 0 424 74 129 535.2 0 129 75 157 535.2 0 157 76 2547 535.2 0 2547 77 1641 535.2 0 1641 78 1192 535.2 0 1192 79 184 535.2 0 184 80 73 535.2 0 73 81 52 535.2 0 52 82 67 535.2 0 67 83 73 535.2 0 73 84 61 535.2 0 61 85 70 535.2 0 70 86 74 535.2 0 74 87 67 535.2 0 67 88 44 535.2 0 44 89 57 535.2 0 57 90 48 535.2 0 48 91 62 535.2 0 62 92 53 535.2 0 53 93 86 535.2 0 86 94 80 535.2 0 80 95 61 535.2 0 61 96 54 535.2 0 54 97 61 535.2 0 61 98 50 535.2 0 50 99 89 535.2 0 89 100 110 535.2 0 110 101 59 535.2 0 59 102 53 535.2 0 53 103 50 535.2 0 50 104 110 535.2 0 110 105 62 535.2 0 62 106 137 535.2 0 137 107 291 535.2 0 291 108 187 535.2 0 187 109 310 535.2 0 310 110 91 535.2 0 91 111 92 535.2 0 92 112 99 535.2 0 99 113 84 535.2 0 84 114 64 535.2 0 64 115 73 535.2 0 73 116 51 535.2 0 51 117 91 535.2 0 91 118 66 535.2 0 66 119 74 535.2 0 74 120 68 535.2 0 68 121 61 535.2 0 61 122 58 535.2 0 58 123 58 535.2 0 58 124 102 535.2 0 102 125 78 535.2 0 78 126 115 535.2 0 115 127 179 535.2 0 179 128 97 535.2 0 97 129 136 535.2 0 136 130 73 535.2 0 73 131 63 535.2 0 63 132 107 535.2 0 107 133 79 535.2 0 79 134 113 535.2 0 113 135 66 535.2 0 66 136 84 535.2 0 84 137 133 535.2 0 133 138 107 535.2 0 107 139 130 535.2 0 130 140 209 535.2 0 209 141 176 535.2 0 176 142 351 535.2 0 351 143 488 535.2 0 488 144 6690 535.2 0 6690 145 4314 535.2 0 4314 146 5471 535.2 0 5471 147 219 535.2 0 219 148 107 535.2 0 107 149 106 535.2 0 106 150 106 535.2 0 106 151 105 535.2 0 105 152 157 535.2 0 157 153 195 535.2 0 195 154 175 535.2 0 175 155 128 535.2 0 128 156 557 535.2 0 557 157 604 535.2 0 604 158 1349 535.2 0 1349 159 217 535.2 0 217 160 224 535.2 0 224 161 306 535.2 0 306 162 551 535.2 0 551 163 1285 535.2 0 1285 164 15014 535.2 0 15014 165 11630 535.2 0 11630 166 8547 535.2 0 8547 167 132 535.2 0 132 168 94 535.2 0 94 169 257 535.2 0 257 170 402 535.2 0 402 171 1445 535.2 0 1445 172 694 535.2 0 694 173 203 535.2 0 203 174 234 535.2 0 234 175 200 535.2 0 200 176 55 535.2 0 55 177 64 535.2 0 64 178 85 535.2 0 85 179 339 535.2 0 339 180 240 535.2 0 240 181 276 535.2 0 276 182 147 535.2 0 147 183 134 535.2 0 134 184 93 535.2 0 93 185 118 535.2 0 118 186 125 535.2 0 125 187 61 535.2 0 61 188 66 535.2 0 66 189 214 535.2 0 214 190 493 535.2 0 493 191 410 535.2 0 410 192 209 535.2 0 209 193 67 535.2 0 67 194 51 535.2 0 51 195 58 535.2 0 58 196 74 535.2 0 74 197 101 535.2 0 101 198 66 535.2 0 66 199 92 535.2 0 92 200 77 535.2 0 77 201 53 535.2 0 53 202 79 535.2 0 79 203 85 535.2 0 85 204 93 535.2 0 93 205 78 535.2 0 78 206 83 535.2 0 83 207 52 535.2 0 52 208 62 535.2 0 62 209 69 535.2 0 69 210 64 535.2 0 64 211 52 535.2 0 52 212 62 535.2 0 62 213 67 535.2 0 67 214 88 535.2 0 88 215 81 535.2 0 81 216 111 535.2 0 111 217 124 535.2 0 124 218 130 535.2 0 130 219 84 535.2 0 84 220 66 535.2 0 66 221 58 535.2 0 58 222 53 535.2 0 53 223 62 535.2 0 62 224 68 535.2 0 68 225 66 535.2 0 66 226 113 535.2 0 113 227 115 535.2 0 115 228 127 535.2 0 127 229 327 535.2 0 327 230 146 535.2 0 146 231 171 535.2 0 171 232 67 535.2 0 67 233 66 535.2 0 66 234 39 535.2 0 39 235 71 535.2 0 71 236 82 535.2 0 82 237 80 535.2 0 80 238 104 535.2 0 104 239 75 535.2 0 75 240 92 535.2 0 92 241 75 535.2 0 75 242 79 535.2 0 79 243 82 535.2 0 82 244 67 535.2 0 67 245 70 535.2 0 70 246 69 535.2 0 69 247 76 535.2 0 76 248 71 535.2 0 71 249 74 535.2 0 74 250 74 535.2 0 74 251 62 535.2 0 62 252 69 535.2 0 69 253 61 535.2 0 61 254 67 535.2 0 67 255 60 535.2 0 60 256 56 535.2 0 56 257 46 535.2 0 46 258 53 535.2 0 53 259 71 535.2 0 71 260 136 535.2 0 136 261 131 535.2 0 131 262 152 535.2 0 152 263 75 535.2 0 75 264 71 535.2 0 71 265 99 535.2 0 99 266 93 535.2 0 93 267 147 535.2 0 147 268 108 535.2 0 108 269 75 535.2 0 75 270 76 535.2 0 76 271 86 535.2 0 86 272 90 535.2 0 90 273 92 535.2 0 92 274 93 535.2 0 93 275 80 535.2 0 80 276 123 535.2 0 123 277 601 535.2 0 601 278 322 535.2 0 322 279 312 535.2 0 312 280 106 535.2 0 106 281 99 535.2 0 99 282 88 535.2 0 88 283 156 535.2 0 156 284 69 535.2 0 69 285 87 535.2 0 87 286 130 535.2 0 130 287 301 535.2 0 301 288 239 535.2 0 239 289 228 535.2 0 228 290 133 535.2 0 133 291 93 535.2 0 93 292 78 535.2 0 78 293 99 535.2 0 99 294 78 535.2 0 78 295 84 535.2 0 84 296 67 535.2 0 67 297 72 535.2 0 72 298 127 535.2 0 127 299 79 535.2 0 79 300 75 535.2 0 75 301 80 535.2 0 80 Finished in 14.40 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 396,135 Reads with adapters: 9,046 (2.3%) Reads that were too short: 198 (0.0%) Reads written (passing filters): 8,848 (2.2%) Total basepairs processed: 88,631,282 bp Total written (filtered): 2,310,807 bp (2.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9046 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 68 0.0 3 0 68 17 7943 0.0 3 7792 148 2 1 18 19 0.0 3 0 17 2 19 3 0.0 3 0 0 1 2 20 13 0.0 3 0 0 0 13 21 3 0.0 3 0 0 0 3 25 1 0.0 3 1 27 2 0.0 3 1 0 0 1 28 5 0.0 3 0 0 0 5 29 1 0.0 3 0 0 0 1 33 11 0.0 3 0 0 0 11 38 11 0.0 3 0 0 0 11 51 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 62 3 0.0 3 0 0 0 3 72 1 0.0 3 1 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 15 0.0 3 0 0 0 15 105 2 0.0 3 0 0 0 2 107 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 109 7 0.0 3 0 0 0 7 112 1 0.0 3 0 0 0 1 114 5 0.0 3 0 0 0 5 115 2 0.0 3 1 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 3 0.0 3 0 0 0 3 124 5 0.0 3 0 0 0 5 125 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 128 3 0.0 3 0 0 0 3 129 636 0.0 3 0 0 2 634 130 13 0.0 3 0 0 0 13 131 3 0.0 3 0 0 0 3 132 73 0.0 3 0 0 2 71 133 1 0.0 3 0 0 0 1 138 1 0.0 3 0 1 139 1 0.0 3 0 0 0 1 144 3 0.0 3 0 0 0 3 145 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 153 3 0.0 3 0 0 0 3 155 9 0.0 3 0 0 0 9 156 2 0.0 3 0 0 0 2 159 1 0.0 3 1 164 4 0.0 3 0 0 0 4 173 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 179 2 0.0 3 0 0 1 1 180 21 0.0 3 0 0 0 21 183 1 0.0 3 0 0 0 1 187 4 0.0 3 0 0 0 4 189 19 0.0 3 0 0 0 19 190 1 0.0 3 0 0 1 191 1 0.0 3 0 0 0 1 201 1 0.0 3 1 207 1 0.0 3 0 0 1 210 1 0.0 3 1 216 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 230 12 0.0 3 0 0 0 12 231 68 0.0 3 0 0 6 62 232 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 239 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.30 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 8,848 Reads with adapters: 91 (1.0%) Reads written (passing filters): 8,848 (100.0%) Total basepairs processed: 2,310,807 bp Total written (filtered): 2,310,486 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 91 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 48.4% C: 2.2% G: 44.0% T: 5.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 43 138.2 0 43 4 48 34.6 0 48