This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.26 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 75,067 (3.4%) Reads that were too short: 14,243 (0.6%) Reads written (passing filters): 60,824 (2.8%) Total basepairs processed: 659,880,795 bp Total written (filtered): 12,726,359 bp (1.9%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 75067 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1484 535.2 0 1484 7 10458 535.2 0 10458 8 7723 535.2 0 7723 9 127 535.2 0 127 10 78 535.2 0 78 11 71 535.2 0 71 12 67 535.2 0 67 13 65 535.2 0 65 14 92 535.2 0 92 15 81 535.2 0 81 16 83 535.2 0 83 17 91 535.2 0 91 18 105 535.2 0 105 19 113 535.2 0 113 20 148 535.2 0 148 21 171 535.2 0 171 22 85 535.2 0 85 23 104 535.2 0 104 24 61 535.2 0 61 25 61 535.2 0 61 26 124 535.2 0 124 27 71 535.2 0 71 28 75 535.2 0 75 29 86 535.2 0 86 30 111 535.2 0 111 31 106 535.2 0 106 32 84 535.2 0 84 33 71 535.2 0 71 34 109 535.2 0 109 35 111 535.2 0 111 36 95 535.2 0 95 37 81 535.2 0 81 38 253 535.2 0 253 39 301 535.2 0 301 40 208 535.2 0 208 41 87 535.2 0 87 42 144 535.2 0 144 43 83 535.2 0 83 44 85 535.2 0 85 45 88 535.2 0 88 46 96 535.2 0 96 47 110 535.2 0 110 48 93 535.2 0 93 49 90 535.2 0 90 50 97 535.2 0 97 51 75 535.2 0 75 52 92 535.2 0 92 53 71 535.2 0 71 54 88 535.2 0 88 55 94 535.2 0 94 56 92 535.2 0 92 57 115 535.2 0 115 58 96 535.2 0 96 59 94 535.2 0 94 60 88 535.2 0 88 61 79 535.2 0 79 62 93 535.2 0 93 63 76 535.2 0 76 64 87 535.2 0 87 65 80 535.2 0 80 66 77 535.2 0 77 67 94 535.2 0 94 68 98 535.2 0 98 69 84 535.2 0 84 70 87 535.2 0 87 71 106 535.2 0 106 72 104 535.2 0 104 73 121 535.2 0 121 74 87 535.2 0 87 75 81 535.2 0 81 76 110 535.2 0 110 77 269 535.2 0 269 78 236 535.2 0 236 79 107 535.2 0 107 80 94 535.2 0 94 81 96 535.2 0 96 82 94 535.2 0 94 83 90 535.2 0 90 84 90 535.2 0 90 85 73 535.2 0 73 86 96 535.2 0 96 87 86 535.2 0 86 88 90 535.2 0 90 89 102 535.2 0 102 90 109 535.2 0 109 91 134 535.2 0 134 92 154 535.2 0 154 93 521 535.2 0 521 94 990 535.2 0 990 95 893 535.2 0 893 96 518 535.2 0 518 97 433 535.2 0 433 98 375 535.2 0 375 99 100 535.2 0 100 100 72 535.2 0 72 101 69 535.2 0 69 102 55 535.2 0 55 103 65 535.2 0 65 104 58 535.2 0 58 105 66 535.2 0 66 106 69 535.2 0 69 107 104 535.2 0 104 108 83 535.2 0 83 109 82 535.2 0 82 110 129 535.2 0 129 111 63 535.2 0 63 112 83 535.2 0 83 113 99 535.2 0 99 114 137 535.2 0 137 115 85 535.2 0 85 116 99 535.2 0 99 117 78 535.2 0 78 118 71 535.2 0 71 119 64 535.2 0 64 120 57 535.2 0 57 121 58 535.2 0 58 122 69 535.2 0 69 123 90 535.2 0 90 124 53 535.2 0 53 125 86 535.2 0 86 126 79 535.2 0 79 127 73 535.2 0 73 128 135 535.2 0 135 129 143 535.2 0 143 130 278 535.2 0 278 131 769 535.2 0 769 132 793 535.2 0 793 133 67 535.2 0 67 134 86 535.2 0 86 135 86 535.2 0 86 136 103 535.2 0 103 137 114 535.2 0 114 138 155 535.2 0 155 139 61 535.2 0 61 140 65 535.2 0 65 141 74 535.2 0 74 142 97 535.2 0 97 143 99 535.2 0 99 144 138 535.2 0 138 145 203 535.2 0 203 146 102 535.2 0 102 147 72 535.2 0 72 148 61 535.2 0 61 149 73 535.2 0 73 150 80 535.2 0 80 151 85 535.2 0 85 152 114 535.2 0 114 153 92 535.2 0 92 154 122 535.2 0 122 155 73 535.2 0 73 156 92 535.2 0 92 157 130 535.2 0 130 158 125 535.2 0 125 159 153 535.2 0 153 160 79 535.2 0 79 161 84 535.2 0 84 162 118 535.2 0 118 163 113 535.2 0 113 164 135 535.2 0 135 165 188 535.2 0 188 166 208 535.2 0 208 167 322 535.2 0 322 168 742 535.2 0 742 169 7258 535.2 0 7258 170 5864 535.2 0 5864 171 3028 535.2 0 3028 172 109 535.2 0 109 173 97 535.2 0 97 174 119 535.2 0 119 175 133 535.2 0 133 176 485 535.2 0 485 177 297 535.2 0 297 178 73 535.2 0 73 179 75 535.2 0 75 180 129 535.2 0 129 181 70 535.2 0 70 182 75 535.2 0 75 183 85 535.2 0 85 184 108 535.2 0 108 185 98 535.2 0 98 186 82 535.2 0 82 187 62 535.2 0 62 188 63 535.2 0 63 189 78 535.2 0 78 190 68 535.2 0 68 191 90 535.2 0 90 192 154 535.2 0 154 193 142 535.2 0 142 194 135 535.2 0 135 195 86 535.2 0 86 196 74 535.2 0 74 197 61 535.2 0 61 198 109 535.2 0 109 199 81 535.2 0 81 200 87 535.2 0 87 201 78 535.2 0 78 202 99 535.2 0 99 203 112 535.2 0 112 204 123 535.2 0 123 205 142 535.2 0 142 206 100 535.2 0 100 207 60 535.2 0 60 208 68 535.2 0 68 209 82 535.2 0 82 210 105 535.2 0 105 211 116 535.2 0 116 212 132 535.2 0 132 213 104 535.2 0 104 214 99 535.2 0 99 215 113 535.2 0 113 216 132 535.2 0 132 217 151 535.2 0 151 218 102 535.2 0 102 219 73 535.2 0 73 220 72 535.2 0 72 221 218 535.2 0 218 222 296 535.2 0 296 223 219 535.2 0 219 224 394 535.2 0 394 225 80 535.2 0 80 226 62 535.2 0 62 227 90 535.2 0 90 228 92 535.2 0 92 229 112 535.2 0 112 230 85 535.2 0 85 231 125 535.2 0 125 232 141 535.2 0 141 233 96 535.2 0 96 234 120 535.2 0 120 235 140 535.2 0 140 236 340 535.2 0 340 237 404 535.2 0 404 238 335 535.2 0 335 239 82 535.2 0 82 240 87 535.2 0 87 241 86 535.2 0 86 242 147 535.2 0 147 243 105 535.2 0 105 244 80 535.2 0 80 245 83 535.2 0 83 246 111 535.2 0 111 247 62 535.2 0 62 248 50 535.2 0 50 249 71 535.2 0 71 250 86 535.2 0 86 251 89 535.2 0 89 252 82 535.2 0 82 253 90 535.2 0 90 254 123 535.2 0 123 255 118 535.2 0 118 256 96 535.2 0 96 257 100 535.2 0 100 258 89 535.2 0 89 259 79 535.2 0 79 260 69 535.2 0 69 261 231 535.2 0 231 262 309 535.2 0 309 263 202 535.2 0 202 264 94 535.2 0 94 265 66 535.2 0 66 266 84 535.2 0 84 267 91 535.2 0 91 268 79 535.2 0 79 269 73 535.2 0 73 270 132 535.2 0 132 271 231 535.2 0 231 272 418 535.2 0 418 273 310 535.2 0 310 274 164 535.2 0 164 275 70 535.2 0 70 276 83 535.2 0 83 277 84 535.2 0 84 278 82 535.2 0 82 279 94 535.2 0 94 280 116 535.2 0 116 281 109 535.2 0 109 282 126 535.2 0 126 283 156 535.2 0 156 284 170 535.2 0 170 285 115 535.2 0 115 286 104 535.2 0 104 287 96 535.2 0 96 288 102 535.2 0 102 289 120 535.2 0 120 290 160 535.2 0 160 291 176 535.2 0 176 292 289 535.2 0 289 293 292 535.2 0 292 294 355 535.2 0 355 295 105 535.2 0 105 296 274 535.2 0 274 297 343 535.2 0 343 298 350 535.2 0 350 299 233 535.2 0 233 300 161 535.2 0 161 301 75 535.2 0 75 Finished in 2.70 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 60,824 Reads with adapters: 8,727 (14.3%) Reads that were too short: 31 (0.1%) Reads written (passing filters): 8,696 (14.3%) Total basepairs processed: 12,726,359 bp Total written (filtered): 2,365,834 bp (18.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8727 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 7 0.0 3 0 0 5 1 1 19 149 0.0 3 0 104 24 14 7 20 8094 0.0 4 7403 513 125 39 14 21 21 0.0 4 3 14 3 1 22 10 0.0 4 1 5 2 1 1 23 330 0.0 4 257 23 6 33 11 24 1 0.0 4 1 26 3 0.0 4 0 0 0 1 2 28 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 41 38 0.0 4 0 0 0 0 38 42 6 0.0 4 0 0 0 0 6 82 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 102 4 0.0 4 0 0 0 0 4 126 2 0.0 4 0 0 0 0 2 127 29 0.0 4 0 0 0 0 29 130 4 0.0 4 0 0 0 0 4 146 1 0.0 4 0 0 0 0 1 149 2 0.0 4 0 0 0 0 2 174 1 0.0 4 0 0 0 0 1 184 3 0.0 4 0 0 0 0 3 196 1 0.0 4 0 0 0 0 1 197 2 0.0 4 0 0 0 0 2 198 2 0.0 4 0 0 0 0 2 204 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 1 1 235 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 287 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.32 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 8,696 Reads with adapters: 1,767 (20.3%) Reads written (passing filters): 8,696 (100.0%) Total basepairs processed: 2,365,834 bp Total written (filtered): 2,345,157 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1767 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.2% C: 10.6% G: 83.5% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 15 135.9 0 15 4 68 34.0 0 68 5 1 8.5 0 1 7 2 0.5 0 2 8 2 0.1 0 2 9 19 0.0 0 2 17 10 130 0.0 1 7 123 11 1503 0.0 1 55 1448 12 12 0.0 1 0 12 13 2 0.0 1 0 2 15 1 0.0 1 0 1 29 1 0.0 1 1 151 2 0.0 1 2 153 3 0.0 1 3 209 3 0.0 1 2 1 224 2 0.0 1 2 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.