This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.04 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 80,093 (3.7%) Reads that were too short: 11,821 (0.5%) Reads written (passing filters): 68,272 (3.1%) Total basepairs processed: 659,880,795 bp Total written (filtered): 13,681,189 bp (2.1%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 80093 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1453 535.2 0 1453 7 9420 535.2 0 9420 8 10019 535.2 0 10019 9 101 535.2 0 101 10 107 535.2 0 107 11 81 535.2 0 81 12 89 535.2 0 89 13 66 535.2 0 66 14 80 535.2 0 80 15 85 535.2 0 85 16 67 535.2 0 67 17 87 535.2 0 87 18 110 535.2 0 110 19 131 535.2 0 131 20 124 535.2 0 124 21 120 535.2 0 120 22 64 535.2 0 64 23 109 535.2 0 109 24 52 535.2 0 52 25 81 535.2 0 81 26 104 535.2 0 104 27 92 535.2 0 92 28 80 535.2 0 80 29 89 535.2 0 89 30 97 535.2 0 97 31 94 535.2 0 94 32 81 535.2 0 81 33 77 535.2 0 77 34 95 535.2 0 95 35 104 535.2 0 104 36 101 535.2 0 101 37 94 535.2 0 94 38 183 535.2 0 183 39 225 535.2 0 225 40 103 535.2 0 103 41 98 535.2 0 98 42 103 535.2 0 103 43 97 535.2 0 97 44 105 535.2 0 105 45 108 535.2 0 108 46 95 535.2 0 95 47 98 535.2 0 98 48 96 535.2 0 96 49 113 535.2 0 113 50 95 535.2 0 95 51 96 535.2 0 96 52 79 535.2 0 79 53 86 535.2 0 86 54 107 535.2 0 107 55 74 535.2 0 74 56 97 535.2 0 97 57 125 535.2 0 125 58 109 535.2 0 109 59 93 535.2 0 93 60 94 535.2 0 94 61 81 535.2 0 81 62 92 535.2 0 92 63 72 535.2 0 72 64 90 535.2 0 90 65 75 535.2 0 75 66 97 535.2 0 97 67 75 535.2 0 75 68 91 535.2 0 91 69 79 535.2 0 79 70 83 535.2 0 83 71 106 535.2 0 106 72 61 535.2 0 61 73 106 535.2 0 106 74 103 535.2 0 103 75 88 535.2 0 88 76 106 535.2 0 106 77 220 535.2 0 220 78 225 535.2 0 225 79 84 535.2 0 84 80 99 535.2 0 99 81 93 535.2 0 93 82 91 535.2 0 91 83 61 535.2 0 61 84 100 535.2 0 100 85 73 535.2 0 73 86 93 535.2 0 93 87 75 535.2 0 75 88 95 535.2 0 95 89 75 535.2 0 75 90 102 535.2 0 102 91 100 535.2 0 100 92 104 535.2 0 104 93 197 535.2 0 197 94 392 535.2 0 392 95 331 535.2 0 331 96 190 535.2 0 190 97 160 535.2 0 160 98 172 535.2 0 172 99 65 535.2 0 65 100 80 535.2 0 80 101 69 535.2 0 69 102 80 535.2 0 80 103 67 535.2 0 67 104 47 535.2 0 47 105 58 535.2 0 58 106 83 535.2 0 83 107 78 535.2 0 78 108 91 535.2 0 91 109 71 535.2 0 71 110 71 535.2 0 71 111 89 535.2 0 89 112 73 535.2 0 73 113 97 535.2 0 97 114 120 535.2 0 120 115 84 535.2 0 84 116 84 535.2 0 84 117 130 535.2 0 130 118 68 535.2 0 68 119 58 535.2 0 58 120 50 535.2 0 50 121 58 535.2 0 58 122 72 535.2 0 72 123 83 535.2 0 83 124 61 535.2 0 61 125 91 535.2 0 91 126 84 535.2 0 84 127 74 535.2 0 74 128 138 535.2 0 138 129 108 535.2 0 108 130 322 535.2 0 322 131 859 535.2 0 859 132 830 535.2 0 830 133 98 535.2 0 98 134 106 535.2 0 106 135 111 535.2 0 111 136 106 535.2 0 106 137 85 535.2 0 85 138 141 535.2 0 141 139 72 535.2 0 72 140 81 535.2 0 81 141 96 535.2 0 96 142 102 535.2 0 102 143 103 535.2 0 103 144 118 535.2 0 118 145 178 535.2 0 178 146 113 535.2 0 113 147 72 535.2 0 72 148 64 535.2 0 64 149 65 535.2 0 65 150 92 535.2 0 92 151 107 535.2 0 107 152 175 535.2 0 175 153 98 535.2 0 98 154 121 535.2 0 121 155 71 535.2 0 71 156 76 535.2 0 76 157 121 535.2 0 121 158 185 535.2 0 185 159 155 535.2 0 155 160 85 535.2 0 85 161 77 535.2 0 77 162 115 535.2 0 115 163 109 535.2 0 109 164 160 535.2 0 160 165 176 535.2 0 176 166 199 535.2 0 199 167 419 535.2 0 419 168 978 535.2 0 978 169 11129 535.2 0 11129 170 8719 535.2 0 8719 171 4595 535.2 0 4595 172 104 535.2 0 104 173 78 535.2 0 78 174 129 535.2 0 129 175 153 535.2 0 153 176 675 535.2 0 675 177 398 535.2 0 398 178 83 535.2 0 83 179 73 535.2 0 73 180 89 535.2 0 89 181 72 535.2 0 72 182 78 535.2 0 78 183 77 535.2 0 77 184 97 535.2 0 97 185 84 535.2 0 84 186 57 535.2 0 57 187 68 535.2 0 68 188 62 535.2 0 62 189 69 535.2 0 69 190 81 535.2 0 81 191 82 535.2 0 82 192 167 535.2 0 167 193 118 535.2 0 118 194 94 535.2 0 94 195 76 535.2 0 76 196 64 535.2 0 64 197 66 535.2 0 66 198 83 535.2 0 83 199 67 535.2 0 67 200 97 535.2 0 97 201 95 535.2 0 95 202 95 535.2 0 95 203 117 535.2 0 117 204 112 535.2 0 112 205 139 535.2 0 139 206 85 535.2 0 85 207 77 535.2 0 77 208 82 535.2 0 82 209 72 535.2 0 72 210 81 535.2 0 81 211 83 535.2 0 83 212 69 535.2 0 69 213 88 535.2 0 88 214 66 535.2 0 66 215 88 535.2 0 88 216 65 535.2 0 65 217 87 535.2 0 87 218 80 535.2 0 80 219 56 535.2 0 56 220 65 535.2 0 65 221 289 535.2 0 289 222 365 535.2 0 365 223 268 535.2 0 268 224 483 535.2 0 483 225 80 535.2 0 80 226 66 535.2 0 66 227 72 535.2 0 72 228 81 535.2 0 81 229 75 535.2 0 75 230 60 535.2 0 60 231 115 535.2 0 115 232 115 535.2 0 115 233 75 535.2 0 75 234 88 535.2 0 88 235 96 535.2 0 96 236 139 535.2 0 139 237 186 535.2 0 186 238 195 535.2 0 195 239 101 535.2 0 101 240 85 535.2 0 85 241 82 535.2 0 82 242 100 535.2 0 100 243 78 535.2 0 78 244 68 535.2 0 68 245 69 535.2 0 69 246 124 535.2 0 124 247 75 535.2 0 75 248 60 535.2 0 60 249 57 535.2 0 57 250 66 535.2 0 66 251 71 535.2 0 71 252 67 535.2 0 67 253 69 535.2 0 69 254 82 535.2 0 82 255 93 535.2 0 93 256 70 535.2 0 70 257 73 535.2 0 73 258 68 535.2 0 68 259 81 535.2 0 81 260 76 535.2 0 76 261 156 535.2 0 156 262 187 535.2 0 187 263 92 535.2 0 92 264 80 535.2 0 80 265 76 535.2 0 76 266 76 535.2 0 76 267 83 535.2 0 83 268 92 535.2 0 92 269 86 535.2 0 86 270 144 535.2 0 144 271 291 535.2 0 291 272 570 535.2 0 570 273 463 535.2 0 463 274 260 535.2 0 260 275 74 535.2 0 74 276 65 535.2 0 65 277 93 535.2 0 93 278 72 535.2 0 72 279 107 535.2 0 107 280 88 535.2 0 88 281 91 535.2 0 91 282 83 535.2 0 83 283 144 535.2 0 144 284 134 535.2 0 134 285 77 535.2 0 77 286 91 535.2 0 91 287 65 535.2 0 65 288 70 535.2 0 70 289 100 535.2 0 100 290 91 535.2 0 91 291 126 535.2 0 126 292 183 535.2 0 183 293 186 535.2 0 186 294 179 535.2 0 179 295 95 535.2 0 95 296 148 535.2 0 148 297 208 535.2 0 208 298 166 535.2 0 166 299 103 535.2 0 103 300 83 535.2 0 83 301 73 535.2 0 73 Finished in 2.60 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 68,272 Reads with adapters: 9,499 (13.9%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 9,483 (13.9%) Total basepairs processed: 13,681,189 bp Total written (filtered): 2,624,014 bp (19.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9499 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 5 16 79 0.0 3 0 78 1 17 8673 0.0 3 8512 156 5 18 23 0.0 3 0 22 1 19 15 0.0 3 1 8 5 1 20 671 0.0 3 646 11 0 14 21 4 0.0 3 2 2 22 3 0.0 3 1 0 2 39 1 0.0 3 1 47 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 67 1 0.0 3 1 76 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 142 1 0.0 3 1 143 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 200 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.33 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 9,483 Reads with adapters: 29 (0.3%) Reads written (passing filters): 9,483 (100.0%) Total basepairs processed: 2,624,014 bp Total written (filtered): 2,623,917 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 29 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 31.0% C: 34.5% G: 27.6% T: 6.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 26 148.2 0 26 4 2 37.0 0 2 11 1 0.0 1 0 1